共查询到20条相似文献,搜索用时 31 毫秒
1.
Ming Chang Qingzhe Jin Yuanfa Liu Ruijie Liu Xingguo Wang 《International Journal of Food Science & Technology》2013,48(12):2474-2479
A photodegradation study of Aflatoxin B1 (AFB1) on peanut surface was performed under UV irradiation at different UV irradiation intensities. With an initial quantity of 10 ng of AFB1, it can be degraded thoroughly within 80 min under the intensity of 800 μw cm?2. The photodegradation pathway of AFB1 on the peanut surface was proposed. Mutagenesis and cytotoxicity were assessed after exposure to different concentrations of AFB1 and the mixtures of photodegradation products on the peanut surface. The results of the Ames and in vitro cytotoxicity assay, providing clues to the assessment of safety issues of UV method applied in AFB1 decontamination, indicate that photodegradation products on the peanut surface has no toxicity, which can be explained by the differences in the chemical nature of the test compounds before and after UV irradiation. 相似文献
2.
Safety evaluation of aflatoxin B1 in peanut oil after ultraviolet irradiation detoxification in a photodegradation reactor
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Enjie Diao Xiangzhen Shen Zheng Zhang Ning Ji Wenwen Ma Haizhou Dong 《International Journal of Food Science & Technology》2015,50(1):41-47
The high incidence of aflatoxin B1 (AFB1) in peanut oil has caused wide public concern in the world. Many studies have verified that ultraviolet (UV) irradiation can degrade AFB1 in foods. A new photodegradation reactor has been developed to study the photodegradation efficiency of AFB1 in peanut oil, and the safety of peanut oil was evaluated after UV irradiation detoxification based on the mutagenicity of Salmonella typhimurium tester strains and cytotoxicity of HepG2 cells. The results showed that AFB1 in peanut oil could be decomposed efficiently using the photodegradation reactor. AFB1 was decreased from 51.96 ± 4.24 to 7.23 ± 0.59 μg kg?1 in 10 min and reduced by 86.08% compared with that of the negative control. The residual AFB1 in peanut oil was far less than the limit level set by Chinese government (20 μg kg?1). The Ames test and cell viability assay revealed that 10 min of UV irradiation reduced significantly the toxicity of AFB1 in peanut oil. All the results suggest that the deleterious effects of AFB1 can be highly reduced by UV irradiation in the photodegradation reactor, and the reactor can be applied in a large scale in detoxification of AFB1 in peanut oil in the oil industry. 相似文献
3.
Ruijie Liu Ming Chang Qingzhe Jin Jianhua Huang Yuanfa Liu Xingguo Wang 《European Food Research and Technology》2011,233(6):1007-1012
Mutagenesis and cytotoxicity were assessed after exposure to different concentrations of AFB1 and the mixtures of photodegradation products in water (Pw). The Ames test, employing Salmonella typhimurium tester strains TA98 and TA100, was employed to evaluate the residual toxicity of Pw, and the results indicated that the mutagenic
activity of UV-treated samples was lower compared with that of untreated samples, but not eliminate absolutely. After exposure
of the HepG2 cells to these compounds for different times and concentrations, the cytotoxicity of Pw decreased approximately
40% compared to AFB1, and a small amount of cells were observed dead by the inverted phase-contrast microscope. The results of the Ames and in
vitro cytotoxicity assay, providing clues to the assessment of safety issues of UV method applied in AFB1 decontamination, indicate that Pw are less toxic than AFB1, which can be explained by the differences in the chemical nature of the test compounds. 相似文献
4.
Cho Yong-Jin Kim Namsoo Kim Chong-Tai Maeng Jin-Soo Pyee Jaeho 《Food science and biotechnology》2012,21(2):597-601
A mathematical model was proposed to quantitatively describe resveratrol induction in harvested grapes. In the model, k1 and k2 were defined, which were the reaction rate constants for induction during direct UV irradiation and for the time-delayed
induction after removing UV irradiation, respectively. During storage after UV irradiation, k2 decreased with time, whereas k1 remained constant. The portion induced by the direct irradiation effect was much more than that induced by the time-delayed
effect. When UV energy of 610.2 mJ/cm2 was applied to ‘Gerbong’ grapes with an initial resveratrol content of 1.15 μg/g, their contents were 8.99 and 9.20 μg/g
at day 1 and 6 during storage at 0°C, respectively. In the same situation, resveratrol content of 8.99 μg/g improved to 10.56
μg/g during storage at 20°C. This approach which enriched a health-functional compound through the modulation of metabolism
after harvest might be a valueadding method for fresh food industry. 相似文献
5.
Shihong Chen Liping Ma Ruo Yuan Yaqin Chai Yun Xiang Chengyan Wang 《European Food Research and Technology》2011,232(1):87-95
In this paper, a novel electrochemical sensor for the detection of H2O2 was proposed based on gold nanochains and prussian blue nanorods (PB@MWCNTs), which were synthesized with multiwall carbon
nanotubes (MWCNTs) as a template. With the introduction of MWCNTs and the gold nanochains, the proposed system shows synergy
among the Prussian blue (PB), MWCNTs, and the gold nanochains, which amplifies the H2O2 sensitivity greatly. A linear range from 1.75 × 10−6 to 1.14 × 10−3 M with a detection limit of 0.5 × 10−6 M (S/N = 3) and a high sensitivity 300 μA mM−1 cm−2 for H2O2 detection is obtained. Moreover, the sensor exhibits good repeatability and stability. 相似文献
6.
Ruijie Liu Mengyao Lu Ruiqi Wang Shanshan Wang Qingzhe Jin 《International Journal of Food Properties》2018,21(1):891-900
The effects and safety of electron beam irradiation (EBI) treatment on the detoxification of aflatoxin B1 (AFB1) in the peanut meal were evaluated in this article. The AFB1 degradation was predominantly affected by both initial AFB1 and water concentrations. The degradation of AFB1 in the selected concentrations (0.5–5 ppm) was proven to follow pseudo first-order reaction kinetics (R2 > 0.95). The AFB1 degradation was faster when the initial concentration was 5 ppm and the moisture content was 21.47%, in comparison with the initial concentration of 1 ppm and 0.5 ppm and the moisture content of 14.32% and 8.74%, respectively. The Ames and cytotoxicity tests were employed to evaluate the residual toxicity of EBI-treated peanut meal. The mutagenic activity of EB-treated samples was completely lost compared with that of untreated samples and the degradation products in peanut meal has almost no cell toxicity. 相似文献
7.
Paula C. Alvito Eric A. Sizoo Cristina M. M. Almeida Hans P. van Egmond 《Food Analytical Methods》2010,3(1):22-30
Infants have a more restricted diet and they generally consume more food on a body weight basis than adults. Therefore, the
significance and potential health risk of any contaminant in foods consumed by infants is increased and diligent attention
must be paid to this particular area. The present study aims to determine the occurrence of aflatoxin M1 (AFM1), aflatoxin B1 (AFB1) and ochratoxin A (OTA) in processed cereal-based foods (flours) and infant formulae (milk powder) available in the Portuguese
market, both sold as conventional and organic origin. Mycotoxin determination was carried out using a method previously applied
to duplicate diet samples. This method employed chloroform extraction, liquid–liquid extraction, immunoaffinity column (IAC)
cleanup and HPLC analysis with fluorescence detection after post-column derivatisation. Quantification limits were 0.014,
0.004 and 0.028 μg kg−1 for AFM1, AFB1 and OTA, respectively. These toxins could only be quantified in 12 of 27 analysed samples (15 positive results): two samples
with AFM1, two samples with AFM1 and OTA, one sample with AFB1 and OTA and seven samples with OTA. Positive results concerned four for AFM1 (26%), one for AFB1 (7%) and ten for OTA (67%). For these samples, contents ranged between 0.017–0.041 μg AFM1 kg−1, 0.034–0.212 μg OTA kg−1, and one sample had a value of 0.009 μg AFB1 kg−1. Considering the presented results, we could provisionally conclude that the presence of these mycotoxins in baby foods does
not constitute a public health problem. These are the first results concerning the occurrence of mycotoxins in marketed baby
foods in Portugal and this is the first study using the HPLC method, proposed for duplicate diets, in baby food sample analysis. 相似文献
8.
近年来,近红外光谱、高光谱等快速无损检测技术,被广泛应用于农产品中黄曲霉毒素B1(Aflatoxin B1, AFB1)的检测。但这些方法主要是根据黄曲霉改变样品本身的结构结合化学计量学方法间接检测样品中AFB1,无特异性。因此,本研究拟利用研发的激光诱导荧光(laser induce fluorescence, LIF)技术获取污染AFB1花生油的荧光光谱,建立精确无损检测花生油中AFB1的定性、定量方法。首先,人工制备7种不同AFB1污染水平的花生油样品,同时设置对照组。其次利用研发的LIF系统从不同角度检测样品的荧光光谱信号。最后,对获取的光谱进行分析。结果显示,三个检测角度的荧光强度在400-800 nm内均随着AFB1污染水平的增加而增加,定性和定量的结果均是当检测荧光角度为90度时表现最优。表明当检测角度为90度时,LIF技术可以作为一种快速、精确的方法来判定花生油中AFB1污染水平。 相似文献
9.
Jennifer C. Hayes John G. Laffey Brian McNeil Neil J. Rowan 《International Journal of Food Science & Technology》2012,47(9):1925-1934
The relationship between prior growth of food‐spoilage yeast in high‐sugar environments and their subsequent survival postpulsed UV (PUV) irradiation was investigated. Test yeast were separately grown to early stationary phase in YPD broth containing increasing concentrations of glucose (1–50% w/v) and were flashed with ≤40 pulses of broad‐spectrum light at lamp discharge energy settings of 3.2, 7.2 and 12.8 J (equivalent to UV doses of 0.53, 1.09 and 3.36 μJ cm?2, respectively) and their inactivation measured. Findings showed that prior growth in high‐sugar conditions (≥30% glucose w/v) enhanced the sensitivity of all nine representative strains of Zygosaccharomyces bailii, Z. rouxii and Saccharomyces cerevisiae yeast to PUV irradiation. Significant differences in inactivation amongst different yeast types also occurred depending on amount of UV dose applied, where the order of increasing sensitivity of osmotically stressed yeast to PUV irradiation was shown to be Z. rouxii, Z. bailii and >S. cerevisiae. For example, a 1.2‐log order difference in CFU mL?1 reduction occurred between Z. bailii 11 486 and S. cerevisiae 834 when grown in 50% w/v sugar samples and treated with the uppermost test UV dosage of 3.36 μJ cm?2, where these two yeast strains were reduced by 3.8 and 5.0 log orders, respectively, after this PUV treatment regime compared to untreated controls. The higher the UV dose applied the greater the reduction in yeast numbers. For example, a 1.0‐, 1.4‐ and 4.0‐log order differences in CFU mL?1 numbers occurred for S. cerevisiae 834 grown in 15% w/v sugar samples and then treated with PUV dose of 0.53, 1.09 and 3.36 μJ cm?2, respectively. These findings support the development of PUV for the treatment of high‐sugar foods that are prone to spoilage by osmotolerant yeast. 相似文献
10.
Analysis of aflatoxin B1 (AFB1), B2 (AFB2), G1 (AFG1) and G2 (AFG2) in 76 edible oil samples (peanut oil, soybean oil, corn embryo oil and blended oil) was performed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The oils were sampled from three areas (Shijiazhuang, Baoding and Tangshan) of Hebei Province of China. AFB1 was detected in 22 samples representing 28.9%, followed by AFB2 (7.89%) and AFG1 (3.95%), while no AFG2 contamination was detected in any samples. AFB1 levels in oil samples ranged 0.14–2.72?µg?kg?1 and AFB2 ranged 0.15–0.36?µg?kg?1, while lower levels of 0.01–0.02?µg?kg?1 for AFG1 were recorded. The paper is part of an on-going investigation of aflatoxin contamination in Chinese edible oils. 相似文献
11.
Ningli Qi Hailong Yu Yangyang Liu Yiting Zhu 《Food Additives and Contaminants: Part B: Surveillance Communications》2019,12(1):45-51
Aflatoxin B1 (AFB1) occurrence in peanut oil samples randomly collected from family workshops in western Guangdong during 2016–2017 (n = 427) was surveyed. AFB1 content was screened by enzyme-linked immunosorbent assay (ELISA) protocol and analytically confirmed with an UPLC-MS/MS method. The limit of detection of the ELISA method was 1.08 μg kg?1. The recovery values ranged from 84.4 to 92.6% with relative standard deviations of 2.2 to 4.8%. AFB1 was quantified in 47 samples (22.5%) with a range of 15.4–49.9 μg kg?1 in 2016 and in 33 samples (15.1%) with 8.8–22.2 μg kg?1 in 2017, respectively. The AFB1 contamination in peanut oil was season-depended in western Guangdong with the worst case in spring (24.2–37.9%). Overall, a significant reduction of AFB1 occurrence was observed in western Guangdong after 2016. It is advisable to control AFB1 in bulk and self-pressed oil from family workshops and regulate it mandatorily if necessary. 相似文献
12.
调查了浙江省食用植物油中黄曲霉毒素B_1(AFB_1)的污染情况。根据浙江省食用植物油生产消费的实际情况,2016年9—12月,采用统计学方法采集151家食用植物油经销企业的花生油、玉米油、大豆油、菜籽油、油茶籽油、葵花籽油、芝麻油、调和油8大类散装油和定型包装油,共1 208个样品,高效液相色谱法测定AFB_1含量。结果表明:22个样品检出AFB_1,检出率为1.8%,超标率为0.0%;其中花生油、葵花籽油、玉米油和调和油检出AFB_1,浙江省特产的油茶籽油和菜籽油未检出AFB_1,散装油中AFB_1检出率(3.8%)是定型包装油(0.9%)的4.22倍,食用植物油样品中AFB_1含量均低于国家限量标准。 相似文献
13.
Aspergillus subolivaceus dextranase is immobilized on several carriers by entrapment and covalent binding with cross-linking. Dextranase immobilized
on BSA with a cross-linking agent shows the highest activity and considerable immobilization yield (66.7%). The optimum pH
of the immobilized enzyme is shifted to pH 6.0 as compared with the free enzyme (pH 5.5). The optimum temperature of the reaction
is resulted at 60 °C for both free and immobilized enzyme. Thermal and pH stability are significantly improved by the immobilization
process. The calculated K
m
of the immobilized dextranase (14.24 mg mL−1) is higher than that of the free dextranase (11.47 mg mL−1), while V
max of the immobilized enzyme (2.80 U μg protein−1) is lower than that of the free dextranase (11.75 U μg protein−1). The immobilized enzyme was able to retain 76% of the initial catalytic activity after 5.0 cycles. 相似文献
14.
The effect of yeast concentration on ultraviolet (UV) inactivation of five strains of Escherichia coli O157:H7 from different sources, inoculated both individually and simultaneously in orange juice, was analyzed and mathematically
modeled. The presence of yeast cells in orange juice decreases the performance of UV radiation on E. coli inactivation. UV absorption coefficients in the juice increased with increasing yeast concentration, and higher UV doses
were necessary to inactivate bacterial strains. UV intensities of I = 3.00 ± 0.3 mW/cm2 and exposure times (t) between 0 and 10 min were applied; radiation doses (energy, E = I × t) ranging between 0 and 2 J/cm2 were measured using a UV digital radiometer. All the tested individual strains showed higher resistance to the treatment
when UV radiation was applied at 4 °C in comparison to 20 °C. UV inactivation of E. coli O157:H7 individual strain was satisfactory fitted with a first order kinetic model. A linear relationship was found between
UV absorptivities and D values (radiation doses required to decrease microbial population by 90%) for each strain. The dose required to reach 5-log
reduction for the most unfavorable conditions that is the most UV resistant strain, and maximum background yeast concentration
was 2.19 J/cm2 at 4 °C (corresponding to 11 min of UV treatment) and 2.09 J/cm2 at 20 °C (corresponding to 10.55 min of UV treatment). When a cocktail of strains was inoculated in orange juice, the logistic
equation was the best model that fits the experimental results due to the deviation from the log-linear kinetics. The UV resistance
between strain cocktail and single strain were mathematically compared. Slopes of the decline curves for strain cocktail at
high UV doses were lower than the slopes of the log-linear equation calculated for the individual strains, even for the most
resistant one. Therefore, microbial inactivation tests using a cocktail of strains are particularly important to determine
the performance of the UV inactivation treatment. 相似文献
15.
Junfeng Li Xiangyi Fang Yucong Yang Xiaoli Cheng Peng Tang 《Food Analytical Methods》2016,9(11):3080-3086
An indirect competitive chemiluminescence (CL) immunoassay based on Fe3O4@SiO2@Au magnetic nanoparticles (Au-SCMPs) has been optimized and developed for the detection of aflatoxin B1 (AFB1). To improve the detection sensitivity and efficiency, this method combines 2′,6′-dimethylcarbonylphenyl-10-sulfopropyl acridinium-9-carboxylate 4′-NHS ester (NSP-DMAE-NHS) as a new kind of high efficient luminescence reagent with a simplified separation procedure based on the optimized Au-SCMPs. Superparamagnetic nanoparticles were coated with different sizes of Au colloids (18, 30, and 50 nm), and their capacities in immobilization of bovine serum albumin (BSA) were investigated. The BSA-AFB1 conjugate (BSA-AFB1) was immobilized on the optimized Au-SCMPs and competed with the free AFB1 for specially binding the NSP-DAME-NHS-labeled anti-AFB1 antibody (mAb-AFB1). After the indirect competitive immunoreactions and magnetic separation, the CL of the nanoparticles was measured by a homemade luminescent measurement system. Under the optimum conditions, the IC50 value was 0.07 ng mL?1 and the limit of detection (LOD) of AFB1 was 0.01 ng mL?1, respectively. The result shows a much lower IC50 and LOD than that typically achieved by ELISA. This proposed immunoassay method was rapid, low-cost, and suitable for the detection of AFB1. 相似文献
16.
David Makarapong Supawan Tantayanon Chupun Gowanit Chaidate Inchaisri 《International Journal of Dairy Technology》2020,73(1):301-305
This study was conducted to investigate the effect of UV‐C irradiation on the reduction of microorganisms in raw milk. The experiments were carried out by varying the flow rates of 2, 4 and 7 L/min with 39W and 48W UV‐C. The number of microorganisms was reduced by 4.70 (39W) and 4.60 log10 CFU/mL (48W) at 120 min. With the residence time of 4.95 s and the UV‐C doses of 98 and 109.9 mJ/cm2 at 253.74 nm, the reduction of microorganism was clearly observed with no significant influence of different UV‐C power settings on the oxidation of milk fat. 相似文献
17.
Response surface methodology was used to predict optimum conditions for hot air roasting of barley grains (temperature, time,
and amount). Antioxidant capacity in the grains was highest under optimum conditions of 250 °C, 63.5 min and 42 g (one and
a half layers). A correlation of R
2 = 0.74 (p < 0.05) was found between 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and total phenolic contents. Ethanol
and aqueous extracts were prepared from grains roasted under optimum conditions and assessed for antioxidant capacity. Antioxidative
compounds in the extracts were then identified using GC–MS. The IC50 value of ethanol extract was significantly lower (11.45 μg mL–1) than that of aqueous extract (33.54 μg mL–1) and α-tocopherol (12.6 μg mL–1) but higher than BHT (9.59 μg mL–1). The same trend was observed in linoleic acid assay. In reducing power, the ethanol extract and α-tocopherol were not significantly
different. Phenolic acids p-hydroxybenzaldehyde, vallinic and gallic acids were identified as the major compounds in the extracts. The results obtained
from this study show that it is possible to optimize antioxidant capacity in barley grains during roasting. 相似文献
18.
Mayeli P. Contreras Ramesh Y. Avula Rakesh K. Singh 《Food and Bioprocess Technology》2010,3(4):629-635
The effect of nano zinc (ZnO) particles in surface enhancement of attenuated total reflection–Fourier transform mid-infrared
spectroscopy (ATR–FTIR) has been studied in butter and spread. Due to the health implications associated with consumption
of trans fats, the studies also included the determination of band corresponding to trans fats of butter/spread in the nano-zinc-treated samples. The FTIR spectra of nano-zinc-treated butter showed enhancement of
bands related to (―C―O, ―CH2―) at wave number 1,238 cm−1 and (O―C―C) band of esters at wave number 1,100 cm−1. Shifting in wave number to 1,150 cm−1 and reduction in its peak intensity was observed with the band corresponding to 1,162 cm−1 (―C―O stretch). Reduction in peak intensity of the bands at about 2,915 and 2,850 cm−1 (C―H groups) was also observed. In the case of spread, nano zinc reduced the peak intensities of FTIR bands at 2,915 cm−1, 2,850 cm−1 (―C―H― (CH2) stretches), 1,746 cm−1 (―C═O ester), 1,465 cm−1 (―C―H (CH2, CH3)), 1,375 cm−1 (―C―H (CH3)) and 1,156 cm−1 (C―O, ―CH2). Trans fats band corresponding to ═C―H stretch (trans bonds, 966 cm−1), was observed in pure butter and spread. Trans fatty acids in butter and spread were quantified with the aid of calibration/validation standards using trielaidin and triolein
and by using partial least squares regression analysis. However, the band at 966 cm−1 was hindered in Zn-treated butter and reduced in Zn-treated spread. 相似文献
19.
Improving significantly the acylation reaction of l-carnitine with conjugated linoleic acid catalyzed by lipase needs to select an efficient and suitable reaction medium that
is not only polar but also hydrophobic. [Bmim]PF6 which is satisfied with the above two requirements was applied as the reaction medium. The optimal reaction conditions were:
Novozyme 435 as the catalyst, 5:1 of molar ratio of fatty acid to l-carnitine, 40 g L−1 lipase, 0.22 aW of initial water activity, 125 g L−1 molecular sieves (they were added within 0–3 h of reaction time), 60 °C of reaction temperature, 200 rpm of rotation speed,
32 h of reaction time. The overall conversion could reach 98.27%. The conversion in [Bmim]PF6 was 2.13 and 1.56 times higher than that in acetonitrile and in solvent-free system, respectively. The lipase in the present
work could be used eight times. [Bmim]PF6 as the reaction medium was better than acetonitrile and solvent-free system. 相似文献
20.
Armando Burgos‐Hernndez Ralph L Price Karen Jorgensen‐Kornman Rebeca Lpez‐García Henry Njapau Douglas L Park 《Journal of the science of food and agriculture》2002,82(5):546-552
The decontamination of aflatoxin B1(AFB1)‐contaminated corn, which is required if the corn is to be suitable for alternative use, by an ammoniation–fermentation integrated process was studied. This process could be used for the production of fuel ethanol from aflatoxin‐containing corn. Different concentrations (0.25, 0.50, 1.00, 1.5 and 2.0% w/w) of ammonium persulphate were tested in the detoxification of AFB1‐contaminated corn during fermentation. In order to increase the decontamination of corn, 0.5 and 1.0% (w/w) azodicarbonamide, benzoyl peroxide and hydrogen peroxide were tested. Peroxides were added at three different stages of the fermentation process: liquefaction, saccharification and fermentation. Levels of AFB1 and ethanol were determined after each fermentation process. Treated corn was tested for mutagenic potential using the Ames test with TA100 tester strain and pure AFB1 as positive control. Addition of 2.0% (w/w) ammonium persulphate caused the highest level of decontamination without affecting ethanol production. Addition of peroxides did not significantly (P < 0.05) increase ethanol production or significantly (P < 0.05) improve the decontamination process. The best processes for decontamination of corn and for ethanol production included the addition of 2.0% (w/w) ammonium persulphate for both and of 1.0 and 0.5% (w/w) benzoyl peroxide respectively. All treated corn samples showed no mutagenic potential. Possible industrial use of these processes is discussed. © 2002 Society of Chemical Industry 相似文献