Nitrogen-15 balance as affected by rice straw management in a rice-wheat rotation in northwest India

The sustainability of the productive rice-wheat systems of Northwest India is being questioned due to the complete removal of straw for animal consumption and fuel, or the burning of straw which has reduced the soil organic matter contents. However, straw incorporation at planting can temporarily reduce the availability of fertilizer-N and reduce crop yields. In a field study on a loamy sand soil, the effect of 6 mg ha⁻¹ rice straw incorporated into the soil 20 or 40 days before sowing (DBS) the wheat was compared with removal or burning of rice straw on the fate and balance of 120 kg ha⁻¹ of 5 atom% ¹⁵N-urea applied to wheat and to a following crop of rice. Wheat grain yield and agronomic efficiency (AE) of applied N (kg grain/kg N applied) were not influenced by rice straw management. However, N uptake (NU), and recovery efficiency (RE) of N by the difference method were lower with rice straw incorporation than with burning. Nitrogen-15 recovery by wheat was highest (41%) when the rice straw was removed or burned and lowest (30.4%) when 30 of the 120 kg N ha⁻¹ was applied at the time of straw incorporation at 20 DBS of wheat. However, this strategy of adding 25% of the urea-N dose at the time of straw incorporation resulted in the highest ¹⁵N losses (45.2%). Inorganic N remaining at harvest in the 0 to 60 cm soil profile, mostly NO₃ ⁻, was 5.5% after wheat and 4.2% after rice. Rice grain yields, NU, and RE were not influenced by rice straw management. Nitrogen-15 losses were similar in rice and wheat (31% with straw removed) despite total irrigation and rainfall inputs of 340 and 32 cm to rice and wheat, respectively. These results suggest to the farmers of northwest India that straw incorporation does not necessarily hurt grain yields, and indicates to researchers that work is still needed to improve N use efficiency in rice and wheat. This revised version was published online in June 2006 with corrections to the Cover Date.     

Influence of nitrogen nutrition and metabolism on ammonia volatilization in plants

Barley (Hordeum vulgare L. cv. Golf) was grown in solution culture with controlled nitrogen availability in order to study the influence of nitrogen nutrition on ammonia emission from the leaves. Ammonia emission measured in cuvettes connected to an automatic NH₃ monitor was close to zero for nitrate grown plants but increased to 0.9–1.3 nmol NH₃ m^-2 leaf area s^-1 after 3–5 days of ammonium nutrition. Increasing concentrations from 0.5 to 10 mM NH₄ ⁺ in the root medium increased NH₃ emission from the shoots, root glutamine synthetase activity and NH₄ ⁺ concentrations in apoplast, xylem sap and bulk tissue, while apoplastic pH values decreased.Inhibition of glutamine synthetase in nitrate grown barley plants by addition of 1 mM methionine sulfoximine (MSO) to the root medium caused ammonia emission to increase 5 to 10-fold after 2–3 hours. At the same time shoot tissue ammonium concentrations started to increase. Addition of an inhibitor of photorespiration, 1 mM pyrid-2-yl hydroxymethane sulfonate (HPMS) reduced this increase in ammonia emission showing a relation between NH₃ emission and photorespiration.Oil seed rape (Brassica napus L. cv. Global) plants grown at 3 different nitogen levels (2N, 4N and 7N) in a sand/soil mixture showed increasing NH₃ compensation points with increasing N level. This increase was highly correlated with increasing NH₄ ⁺ concentrations in the leaf apoplast and total leaf tissue. The NH₃ compensation points could be succesfully predicted on basis of the pH and NH₄ ⁺ concentration in the leaf apoplast.     

Accelerated soil mineralization,nitrification, and revegetation of abandoned fields due to the removal of crop-soil phytotoxicity

In an abandoned corn field, clear-cutting of crop vegetation increased the productivity, species richness, and nonannuals in the following years after abandonment, as compared to the control plots from which crop vegetation was not removed. The increase in plant growth was apparently due to the elimination of allelopathic chemicals from the soil, which normally are released from the standing crop. Removal of vegetation also increased the soil mineralization of Ca²⁺, Mg²⁺, K⁺, NH₄ ⁺ and NO₃ ^–-N. This situation encouraged species having higher mineral requirements to rapidly invade the fields in the successive years. Clear-cutting also increased the nitrification process by removing the inhibitors of nitrification. The number ofNitrosomonas was always significantly higher in the harvested plots as compared to unharvested plots. Phenolic phytotoxins were isolated from the crop residue and soil. Further, these phytotoxins were significantly higher in the unharvested crops as compared to clear-cut plots, in most samples. Whatever the direct or indirect additional explanation for increased biomass, nonannuals and richness in successive years, it is clear that the removal of standing crop has a definite influence.     

Nitrogen dynamics in paddy field as influenced by free-air CO2 enrichment (FACE) at three levels of nitrogen fertilization

As part of a FACE (free-air CO₂ enrichment) experiment in a rice paddy field in Shizukuishi (Iwate Prefecture, Japan), studies were conducted to determine the effects of elevated CO₂ on N dynamics at three levels of N application. Rice plants were grown under ambient CO₂ or ambient + 200 ppmV CO₂ conditions throughout the growing season in an Andosol soil with each treatment having 4 replicated plots. Three levels of N fertilizer (high, standard and low; 15, 9 and 4 gN m^–2, respectively) were applied to examine different N availability under both CO₂ conditions. Soil samples were collected at 4 different times from upper and lower soil layers (0–1 cm and 1–10 cm soil depths, respectively) and analyzed for microbial biomass N (B_N), mineralizable N (Min. N) and NH₄ ⁺-N in soil. Plant sampling was also done at 3 different times during the growing season to determine the N uptake by plant. Elevated CO₂ significantly increased B_N and Min. N in the upper soil layer at harvest by 25–42% and 18–24%, respectively, compared to ambient CO₂, regardless of N application rate. In low N soil, these significant increases were also observed at the ripening stage. In addition, elevated CO₂ only significantly increased the NH₄ ⁺-N in the upper soil layer at harvest in low N soil compared to ambient CO₂. The N uptake was not significantly affected by CO₂ treatment. These results indicate that elevated CO₂ had significant positive influence on B_N and Min. N in the upper soil layer in paddy soil at the later period of the cropping season at all levels of N application rates, but only at low levels of application rate on NH₄ ⁺-N.     

Impact of organic residue management on nitrogen use efficiency in an annual rice cropping sequence of lowland Central Thailand

Field experiments were conducted in Central Thailand under a rice–fallow–rice cropping sequence during consecutive dry and wet seasons of 1998 to determine the impact of residue management on fertilizer nitrogen (N) use. Treatments consisted of a combination of broadcast urea (70 kg N ha^–1) with rice straw (C/N 67) and rice hull ash (C/N 76), which were incorporated into the puddled soil 1 week before transplanting at a rate of 5 Mg ha^–1. Nitrogen-15 balance data showed that the dry season rice recovered 10 to 20% of fertilizer N at maturity. Of the applied N, 27 to 36% remained in the soil. Loss of N (unaccounted for) from the soil–plant system ranged from 47 to 54% of applied N. The availability of the residue fertilizer N to a subsequent rice crop was only less than 3% of the initial applied N. During both season fallows NO₃-N remained the dominant form of mineral-N (NO₃+NH₄) in the aerobic soil. In the dry season grain yield response to N application was significant (P=0.05). Organic material sources did not significantly change grain yield and N accumulation in rice. In terms of grain yields and N uptake at maturity, there was no significant residual effect of fertilizer N on the subsequent rice crop. The combined use of organic residues with urea did not improve N use efficiency, reduced N losses nor produced higher yields compared to urea alone. These results suggested that mechanisms such as N loss through gaseous N emissions may account for the low fertilizer N use efficiency from this rice cropping system. Splitting fertilizer N application should be considered on the fertilizer N use from the organic residue amendment.     

The fate of algal nitrogen in a flooded soil system

Algal N labelled with ¹⁵N added to a flooded soil in laboratory columns without plants was studied to determine the changes over time in the fate of N assimilated by algae and to study how its fate is affected by (a) exclusion of light simulating complete closure of the rice canopy, and (b) addition of fertilizer-NH₄ ^*. In the light but with no added fertilizer-N there was little net mineralization of the added algal N during the first 4 weeks, but after 8 weeks 42% had been mineralized, of which 95% was denitrified. Exclusion of light caused net mineralization to proceed more rapidly in the first 4 weeks due to the death of algal cells and lowered reassimilation. After 8 weeks 51% had been mineralized, of which 54% was denitrified, 16% volatilized and 30% was present as KCl exchangeable NH₄ ⁺-N. Application of fertilizer-NH₄ ⁺ apparently caused mineralization of 25% of the algal N within one week but the results were probably affected by pool substitution in which labelled N mineralized to NH₄ ⁺-N was diluted with fertilizer – NH⁺ ₄ and then immobilized leaving more labelled NH₄–N in the mineral pool. After 8 weeks, 42% of algal N had been mineralized, of which 69% was estimated to have been denitrified, 19% lost through NH₃ volatilization and 12% remained as extracted NH₄ ⁺+NO^- ₃. Uptake of N by a rice crop would reduce the gaseous losses. Algal N was mineralized quickly enough to be available during the growing season of a rice crop and, depending on field conditions, algae may have a role in assimilating N and protecting it from loss as well as being a major driving force for NH₃ volatilization through diurnal increases in pH.     

Effect of soil clay mineralogy on the efficiency of ammonium sulfate in flooded rice

The effect of soil clay mineralogy on the efficiency of (NH₄)₂SO₄ in flooded rice was investigated in a greenhouse pot trial with four clayey soils of diverse clay mineralogies (x-ray amorphous, montmorillonite, vermiculite, beidellite). KCl (75 kg K ha^–1) and triple superphosphate (25 kg P ha^–1) were incorporated in the soil with and without (NH₄)₂SO₄ (100 kg N ha^–1) before transplanting 1-week-old IR-36 rice seedlings which were then grown to maturity under flooded conditions. Efficiency of (NH₄)₂SO₄ was inferred from the response of agronomic characteristics such as tiller number, height, grain and straw yields to NH₄ fertilization.The results showed greatest efficiency of (NH₄)₂SO₄ on the x-ray amorphous soil, followed by montmorillonitic soil; efficiency was much lower on the vermiculitic and negligible on the beidellitic soil.Soil clay mineralogy may be an important factor in the reduced efficiency of NH₄ (or NH₄-forming) fertilizers in certain rice soils.     

Impact of rice straw management practices on yield,nitrogen uptake and soil properties in a wheat-rice rotation in northern India

To study the long term effects of rice straw management practices in a wheat-rice rotation, experiments were started from the dry season (Nov–May) of 1984 to wet season (July–Nov) of 1989. Each year, six straw management practices, viz. control (C), straw incorporation (SI), straw mulch (SM), straw burning (SB), animal manure incorporation (AM), and straw and animal manure incorporation together (SI+AM) were imposed to wheat crop and their subsequent residual effect was studied on the following rice crop under three levels of N, viz. 0, 60 and 120 kg N ha^–1. The rate of straw and animal manure used was 5 t ha^–1 on dry weight basis.The wheat yield and N uptake did not vary significantly under control and SB throughout the experimental period. But, the production level of wheat and N uptake were consistently higher under AM and SM over these two treatments. The SI+AM which had significantly lower wheat yields and N uptake over the AM during the first crop, became equal to that of AM and SM during the second and third crops, and out yielded these two treatments from the fourth crop onward. Straw incorporation which produced wheat yield and N uptake even less than control and SB during the first two crops, resulted in wheat yield and N uptake equivalent to AM and SM from the fourth crop onward.None of the straw management practices had residual effects on the yields and N uptake during the first rice crop, except SM which reduced the rice yields and N uptake in the first two crops. However, AM and SI+AM in the second crop; AM, SI+AM and SI in the third crop; and AM, SI+AM, SI and SM from the fourth crop onward had significant and positive residual effects on rice yields and N uptake. Among these four treatments, SI+AM produced residual effects which were significantly higher than the remaining three treatments. Considering the production levels of wheat and rice, SI+AM treatment resulted in savings of 60 kg N ha^–1 each for wheat and rice.After five years of experimentation, the maximum soil build-up of organic carbon; available N, P and K; and DTPA-extractable Zn, Cu, Fe and Mn was observed under SI+AM, followed by AM and SM and it was minimal under SB and control treatments. The treatments of AM and SI+AM also resulted in a high percentage of water-stable aggregates of 70.25 mm in diameter (80.9%), larger mean weight diameter (0.82 mm), higher porosity (54.2%) and lower bulk density (1.19 Mg m^–3).     

Nitrogen utilization by lowland rice as affected by fertilization with urea and green manure

Field studies were conducted during two consecutive wet seasons in flooded rice (Oryza sativa L.) to determine the effect of green manure on urea utilization in a rice-fallow-rice cropping sequence. Replicated plots were fertilized with 60 to 120 kg of urea N ha^–1 in three split applications (50, 25 and 25%) with or without incorporation of dhaincha (Sesbania aculeata L.) (100 kg N ha^–1). During the first crop only 31 to 44% of the urea added was used by the rice. Incorporatingin situ grown dhaincha (GM) into the soil at transplanting had little effect on urea utilization. Forty-four to 54% of the N added was not recovered in the soil, rice crop, or as nitrate leachate during the first cropping season. Incorporation of GM had no effect on fertilizer N recovery. Only about 2% of the urea N added to the first rice crop was taken up by the second rice crop and, as in the first crop, the GM had little effect on residual N, either in amount or utilization.     

Effects of soil fumigation and N source on soil inorganic N and tomato growth

Soil fumigation, commonly used in vegetable production, may alter the rate of nitrification, affecting availability of N for crop use. The objective of this research was to examine effects of soil fumigation and N fertilizer source on tomato growth and soil NO₃–N and NH₄–N in field production. Experiments 1 and 2 included application of methyl bromide at 420 kg ha^-1 to a Norfolk sandy loam (fine loamy siliceous thermic Typic Kandiudult) in combination with preplant applications of calcium nitrate, ammonium nitrate, and ammonium sulfate at 144 kg N ha^-1. An additional fumigant, metam-sodium, was included in the second experiment at 703 L ha^-1 (268 kg sodium methyldithiocarbamate ha^-1). Experiment 3 included methyl bromide and metam-sodium, with ammonium sulfate as the sole source of N applied at 144 kg N ha^-1. In the first two studies, fumigants had little or no effect on soil NH₄–N or NO₃–N concentration. Tomato plants were larger and fruit yield was greater in fumigated plots, but there were few growth or yield responses to N source. In the third experiment, fumigants increased concentration of soil NO₃–N and NH₄–N at 16 days after fumigation (DAF), however, there was no effect on nitrification owing to fumigants. It appears that N source selection to overcome inhibition of nitrification is not necessary in plant production systems that involve fumigation     

Autointoxication mechanism ofOryza sativa

The phytotoxicity produced during decomposition of rice straw in soil was evaluated under both constant and changing temperature conditions. Bioassay tests showed that the aqueous extract from a soilstraw mixture after incubation at constant temperature was more than twice as phytotoxic as the extract from soil incubated alone. The phytotoxicity was highest at 20–25 ° C. Temperatures above 25 ° C enhanced rice straw decomposition and also degraded the phytotoxic substances more rapidly. After incubation of soil mixtures under changing temperature regimes in a phytotron, the phytotoxicy of the soil aqueous extracts increased in the following order: soil alone < soil + fertilizer < soil + straw < soil + straw + fertilizer. Growth inhibition of lettuce or rice seedlings was also at the highest at the temperature range of 25–30 ° C irrespective of the direction of temperature changes from either low to high or vice versa. Five phytotoxic phenolics,p-hydroxybenzoic, vanillic,p-coumaric, syringic, and ferulic acids, were obtained from both the aqueous extract and residue of the incubated soil samples and were quantitatively estimated by chromatography. The amount of phytotoxins found in various soil mixtures followed the same increasing order as that found by the seed bioassay test. Although no definite distribution pattern of the phenolics in the incubated soil samples can be attributed to temperature variations, the amount of the phenolics was likely higher in the samples incubated at 25 ° C than at either 15 ° C or 35 ° C. The quantity of toxins released during decomposition of rice straw in soil reached highest levels six weeks after incubation and gradually disappeared after twelve weeks.Paper No. 242 of the Scientific Journal Series of the Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China. This study was supported by a project under the R.O.C.-U.S.A. Cooperative Science Program.     

Process rates of nitrogen cycle in uppermost topsoil after harvesting in no-tilled and ploughed agricultural clay soil

No-till is considered an agricultural practice beneficial for the environment as soil erosion is decreased compared to ploughed soils. For on overall evaluation of the benefits and disadvantages of this crop production method, understanding the soil nutrient cycle is also of importance. The study was designed to obtain information about gross soil nitrogen (N) process rates in boreal no-tilled and mouldboard ploughed spring barley (Hordeum vulgare L.) fields after autumn harvesting. In situ soil gross N transformation process rates were quantified for the 5 cm topsoil in 9 days’ incubation experiment using ¹⁵N pool dilution and tracing techniques and a numerical ¹⁵N tracing model. Gross N mineralization into ammonium (NH₄ ⁺) and NH₄ ⁺ immobilization were the most important N transformation processes in the soils. The gross mineralization rate was 14% and NH₄ ⁺ immobilization rate 64% higher in no-till than in ploughing. Regardless of the faster mineralization, the gross rate of NH₄ ⁺ oxidation into nitrate (NO₃ ^?) in no-till was one order of magnitude lower compared the ploughing. The results indicate that the no-tilled soils have the potential to decrease the risk for NO₃ ^? leaching due to slower NH₄ ⁺ oxidation.     

Quantitative dependence of methane emission on soil properties

To identify the key soil parameters influencing CH₄ emission from rice paddies, an outdoor pot experiment with a total of 18 paddy soils was conducted in Nanjing Agricultural University during the 2000 rice growing season. The seasonal average rate of CH₄ emission for all 18 soils was 6.42±2.70 mg m^–2 h^–1, with a range of 1.96 to 11.06 mg m^–2 h^–1. Correlation analysis indicated that the seasonal average of CH₄ emission was positively dependent on soil sand content and negatively on total N as well as NH₄ ⁺-N determined before rice transplanting. Copper content of soils had a significant negative impact on CH₄ emission. No clear relationship existed between CH₄ emission and soil carbon content. In addition, soil type cannot explain the variability in CH₄ emission. Soil parameters influencing CH₄ emission were different as rice growth and development proceeded. A further investigation suggested that the seasonal average rate of CH₄ emission could be quantitatively determined by a linear combination of soil NH₄ ⁺-N, available copper, the ratio of available to total sulphur, and the ratio of available to total iron. Moreover, the average rates of CH₄ emission in the vegetative, reproductive and ripening stages could be also respectively described by a linear combination of different soil variables.     

Crop production,nitrogen recovery and water use efficiency in rice–wheat rotation as affected by non-flooded mulching cultivation (NFMC)

Non-flooded mulching cultivation (NFMC) for lowland rice, as a novel water-saving technique, has been practiced in many areas of China since the 1990s. However, the information on NFMC effects on crop production, nitrogen and water use in rice–wheat rotations is still limited. A field experiment using ¹⁵N-labeled urea was conducted to evaluate the impacts of NFMC on crop yield, fertilizer N recovery and water use efficiency in rice–wheat rotations. Plastic film mulching (PM), and wheat straw and plastic film double mulching (SPM) resulted in the same rice grain yield (7.2 t ha^–1) while wheat straw mulching (SM) and no mulching (NM) led to 5 and 10% yield reduction, compared with rice under traditional flooding (TF). In the rice–wheat rotation, crop productivity in PM, SM or SPM was comparable to that in TF but greater than in NM. Weed growth and its competition with rice for nitrogen were considered the main reason that led to yield decline in NM. Compared with TF, NFMC treatments did not obviously affect fertilizer N recoveries in plant and soil in both rice and wheat seasons. The total fertilizer N recoveries in crop, weed and soil in all treatments were only 39–44% in R–W rotations, suggesting that large N losses occurred following one basal N application for each growing season. Water use efficiency, however, was 56–75% greater in NFMC treatments than in TF treatment in the R–W rotation. The results revealed that NFMC (except NM) can produce comparable rice and wheat yields and obtain similar fertilizer N recovery as TF with much less water consumption.     

Effect of green manure on the yield,N uptake and floodwater properties of a flooded rice,wheat rotation receiving15N urea on a highly permeable soil

Field studies were conducted for two years on a rapidly percolating loamy sand (Typic Ustochrept) to evaluate the effect of green manure (GM) on the yield,¹⁵N recovery from urea applied to flooded rice, the potential for ammonia loss and uptake of residual fertilizer N by succeeding crops. The GM crop ofSesbania aculeata was grownin situ and incorporated one day before transplanting rice. Urea was broadcast in 0.05 m deep floodwater, and incorporated with a harrow. Green manure significantly increased the yield and N uptake by rice and substituted for a minimum of 60 kg fertilizer N ha^–1. The recovery of fertilizer N as indicated by¹⁵N recovery was higher in the GM + urea treatments. The grain yield and N uptake by succeeding wheat in the rotation was slightly higher with GM. The recovery of residual fertilizer N as indicated by the¹⁵N recovery in the second, third and fourth crops of wheat, rice and wheat was only 3, 1 and 1 per cent of the urea fertilizer applied to the preceding rice crop. Floodwater chemistry parameters showed that the combined use of the GM and 40 kg N ha^–1 as urea applied at transplanting resulted in a comparatively higher potential for NH₃ loss immediately after fertilizer application. The actual ammonia loss as suggested by the¹⁵N recoveries in the rice crop, however, did not appear to be appreciably larger in the GM treatment. It appeared the ammonia loss was restricted by low ammoniacal-N concentration maintained in the floodwater after 2 to 3 days of fertilizer application.     

Nitrogen fertigation of greenhouse-grown strawberries

Two greenhouse experiments were conducted with strawberries (Fragaria ananassa) grown in plastic pots filled with 12 kg of soil, and irrigated by drip to evaluate the effect of 3 N levels and 3 N sources. The N levels were 3.6, 7.2 or 10.8 mmol Nl^–1 and the N sources were urea, ammonium nitrate and potassium nitrate for supplying NH₄/NO₃ in mmol Nl^–1 ratios of 7/0, 3.5/3.5 or 0/7, respectively. Both experiments were uniformly supplied with micronutrients and 1.7 and 5.0 mmoll^–1 of P and K, respectively. The fertilizers were supplied through the irrigation stream with every irrigation. The highest yield was obtained with the 7.2 mmol Nl^–1 due to increase in both weight and number of fruits per plant. With this N concentration soil ECe and NO₃-N concentration were kept at low levels. Total N and NO₃-N in laminae and petioles increased with increasing N level. With the N sources the highest yield was obtained with urea due to better fruit setting. The N source had no effect on soil salinity and residual soil NO₃-N; residual NH₄-N in the soils receiving urea and ammonium nitrate were at low levels.     

Simulating water and nitrogen behaviour in soils cropped with winter wheat

The SWATNIT model [26], predicting water and nitrogen transport in cropped soils, was evaluated on experimental data of winter wheat for different N treatments. The experiments were monitored at three different locations on different soil types in the Netherlands. Crop growth was simulated using the SUCROS model [11] which was integrated in the SWATNIT model. Both water and nitrogen stress were incorporated. Except for the soil hydraulic properties, all model parameters were taken from literature. The model performance was evaluated on its capability to predict soil moisture profiles, nitrate and ammonia profiles, the time course of simulated total dry matter production and LAI; and crop N-uptake. Results for the simulations of the soil moisture profile indicate that the soil hydraulic properties did not reflect the actual physical behaviour of the soil with respect to soil moisture. Good agreement is found between the measured and simulated nitrate and ammonia profiles. The simulation of the nitrate content of the top layer at Bouwing was improved by increasing the NH ₄ ⁺ -N-distribution coefficient thereby improving the simulation of the NH ₄ ⁺ -N-content in this layer. Deviations between simulated and measured nitrate concentrations also occurred in the bottom layers (60–100 cm) of the soil profile. The phreatic ground water might influence the nitrate concentrations in the bottom layers. Concerning crop growth modelling, improvements are needed with respect to the partitioning of total dry matter production over the different plant organs in function of the stress, the calculation of the nitrogen stress and the total nitrogen uptake of the crop through a better estimate of the N-demand of the different plant organs.     

Effects of different levels of chemical Nitrogen (urea) onAzolla and Blue-green algae intercropping with rice

Application of higher levels (60 and 90 kg N ha^–1) of nitrogen fertilizer (Urea) inhibited the growth ofAzolla pinnata (Bangkok) and blue-green algae (BGA) though the reduction was more in BGA thanAzolla. Inoculation of 500 kg ha^–1 of freshAzolla 10 days after transplanting (DAT) in the rice fields receiving 30, 60 and 90 kg N ha^–1 as urea produced an average of 16.5, 15.0 and 13.0 t ha^–1 fresh biomass ofAzolla at 30 DAT, which contained 31, 31 and 27 kg N ha^–1, respectively. The dry mixture of BGA (60%Aulosira, 35%Gloeotrichia and 5% other BGA on fresh weight basis) inoculated in rice field 3 DAT at a rate of 10 kg ha^–1 showed a mat formation at 80 DAT with an average fresh biomass of 8.0, 5.8 and 4.2 t ha^–1 containing 22, 17 and 12 kg N ha^–1, respectively with those N fertilizer doses.Application ofAzolla showed positive responses to rice crop by increasing the panicle number and weight, grain and straw yields and nitrogen uptake in rice significantly at all the levels of chemical nitrogen. But, the BGA inoculation had a significant effect on the grain and straw yields only during the dry season in the treatment where 30 kg N was applied. During the wet season and in the other treatments performed during the dry season no significant increase in yields, yield components and N uptake were observed with BGA.The intercropping ofAzolla and rice in combination with 30, 60 and 90 kg N ha^–1 as urea showed the yields, yield attributes and nitrogen uptake in rice at par with those obtained by applying 60, 90 and 120 kg N ha^–1 as urea, respectively but, the BGA did not. The analysis of soil from rice field after harvest showed thatAzolla and BGA intercropping with rice in combination with chemical fertilizer significantly increased the organic carbon, available phosphorus and total nitrogen of soil.     

Determination of the nitrogen compound balance between the atmosphere and a Norway spruce forest ecosystem

Wet, throughfall and stemflow deposition measurements of nitrogen compounds (NH₄ ⁺- and NO₃ ^–-ions) were carried out in a Norway spruce forest in Hungary, together with direct dry deposition and emission estimates (NH₃, NO, NO₂, HNO₃, N₂O gases, NH₄ ⁺ and NO₃ ^– particles). The total deposition of nitrogen compounds from the atmosphere to the forest ecosystem, estimated as the sum of the wet deposition and the measured dry flux, was 1.9 g N m^–2 yr^–1 for the examined period (1996–1998). The net deposition (difference of the deposition and the emission) was 1.8 g N m^–2 yr^–1. About 61% of the deposition is due to dry deposition processes. Ammonia gas dominates in the dry deposition (48%). Soil emission of nitric oxide (NO) and nitrous oxide (N₂O) constitutes only 5% of the total (wet and dry) deposition. From the comparison of directly measured dry deposition figures and the dry deposition calculated as the difference of the throughfall plus stemflow and wet deposition, it is probable that around 60% of the dry deposited nitrogen compounds (36% of the total, dry and wet deposition) are taken up by stomata, mostly in gaseous form. The remaining part (64%) of the deposited nitrogen compounds is leached to the ground where it is partly taken up by the root system, takes part in the soil mineralisation processes, or leaves the ecosystem by ground or surface water run-off.