Thermal and hydrologic suitability of Lake Erie and its major tributaries for spawning of Asian carps

Bighead carp Hypophthalmichthys nobilis, silver carp H. molitrix, and grass carp Ctenopharyngodon idella (hereafter Asian carps) have expanded throughout the Mississippi River basin and threaten to invade Lakes Michigan and Erie. Adult bighead carp and grass carp have been captured in Lake Erie, but self-sustaining populations probably do not exist. We examined thermal conditions within Lake Erie to determine if Asian carps would mature, and to estimate time of year when fish would reach spawning condition. We also examined whether thermal and hydrologic conditions in the largest tributaries to western and central Lake Erie were suitable for spawning of Asian carps. We used length of undammed river, predicted summer temperatures, and predicted water velocity during flood events to determine whether sufficient lengths of river are available for spawning of Asian carps. Most rivers we examined have at least 100 km of passable river and summer temperatures suitable (> 21 C) for rapid incubation of eggs of Asian carps. Predicted water velocity and temperature were sufficient to ensure that incubating eggs, which drift in the water column, would hatch before reaching Lake Erie for most flood events in most rivers if spawned far enough upstream. The Maumee, Sandusky, and Grand Rivers were predicted to be the most likely to support spawning of Asian carps. The Black, Huron, Portage, and Vermilion Rivers were predicted to be less suitable. The weight of the evidence suggests that the largest western and central Lake Erie tributaries are thermally and hydrologically suitable to support spawning of Asian carps.     

Status of the major aquaculture carps of China in the Laurentian Great Lakes Basin

There is concern of economic and environmental damage occuring if any of the four major aquacultured carp species of China, black carp Mylopharyngodon piceus, bighead carp Hypophthalmichthys nobilis, silver carp H. molitrix, or grass carp Ctenopharyngodon idella, were to establish in the Laurentian Great Lakes. All four are reproducing in the Mississippi River Basin. We review the status of these fishes in relation to the Great Lakes and their proximity to pathways into the Great Lakes, based on captures and collections of eggs and larvae. No black carp have been captured in the Great Lakes Basin. One silver carp and one bighead carp were captured within the Chicago Area Waterway System, on the Great Lakes side of electric barriers designed to keep carp from entering the Great Lakes from the greater Mississippi River Basin. Three bighead carp were captured in Lake Erie, none later than the year 2000. By December 2019, at least 650 grass carps had been captured in the Great Lakes Basin, most in western Lake Erie, but none in Lake Superior. Grass carp reproduction has been documented in the Sandusky and Maumee rivers in Ohio, tributaries of Lake Erie. We also discuss environmental DNA (eDNA) results as an early detection and monitoring tool for bighead and silver carps. Detection of eDNA does not necessarily indicate presence of live fish, but bigheaded carp eDNA has been detected on the Great Lakes side of the barriers and in a small proportion of samples from the western basin of Lake Erie.     

An evaluation of statistical methods for estimating abundances of migrating adult sea lamprey

We evaluated statistical methods for estimating abundances of adult sea lamprey (Petromyzon marinus) migrating in Great Lakes tributaries. The sea lamprey is the target of a basin-wide, bi-national control program. Abundance estimates from mark-recapture data are used to evaluate program success and the efficiency of sea lamprey trapping. Recent tracking studies suggested the mark-recapture estimates of abundance could be biased. We compared four estimators of abundance using stratified (weekly) mark-recapture data for 19?years of trapping in the St. Marys River. Abundances from the pooled Petersen estimator were similar to abundances from the stratified Schaefer and stratified Petersen estimators, but substantially lower than abundances from a stratified Bayesian P-spline estimator. In simulations of virtual populations, pooled Petersen and Bayesian P-spline estimates were similar across a range of conditions where catchability differed between marked and unmarked lamprey and changed over the trapping season, with one exception. Abundances from the Bayesian P-spline estimator were strongly positively biased when catchability of marked lamprey increased over the season, while catchability of unmarked lamprey did not. Estimates from both estimators were negatively biased when lamprey displayed consistent individual differences in catchability and positively biased when a proportion of marked lamprey fell back. Discrepancies between mark-recapture and tracking studies cannot be reconciled by the choice of abundance estimator, but could be an outcome of bias due to individual differences in catchability. Sea lamprey managers could also switch from the stratified Schaefer to the pooled Petersen estimator to simplify field operations without losing accuracy and precision.     

Contribution of manipulable and non-manipulable environmental factors to trapping efficiency of invasive sea lamprey

We identified aspects of the trapping process that afforded opportunities for improving trap efficiency of invasive sea lamprey (Petromyzon marinus) in a Great Lake's tributary. Capturing a sea lamprey requires it to encounter the trap, enter, and be retained until removed. Probabilities of these events depend on the interplay between sea lamprey behavior, environmental conditions, and trap design. We first tested how strongly seasonal patterns in daily trap catches (a measure of trapping success) were related to nightly rates of trap encounter, entry, and retention (outcomes of sea lamprey behavior). We then tested the degree to which variation in rates of trap encounter, entry, and retention were related to environmental features that control agents can manipulate (attractant pheromone addition, discharge) and features agents cannot manipulate (water temperature, season), but could be used as indicators for when to increase trapping effort. Daily trap catch was most strongly associated with rate of encounter. Relative and absolute measures of predictive strength for environmental factors that managers could potentially manipulate were low, suggesting that opportunities to improve trapping success by manipulating factors that affect rates of encounter, entry, and retention are limited. According to results at this trap, more sea lamprey would be captured by increasing trapping effort early in the season when sea lamprey encounter rates with traps are high. The approach used in this study could be applied to trapping of other invasive or valued species.     

Salinity tolerance of the invasive round goby: Experimental implications for seawater ballast exchange and spread to North American estuaries

The Eurasian round goby (Neogobius melanostomus) invaded the freshwater North American Great Lakes in ~ 1990 via accidental introduction from ballast water discharge. Its genotypes in the Great Lakes traced to estuaries in the northern Black Sea, where the round goby flourishes in a variety of salinities to 22 parts per thousand (ppt). To prevent further introductions, U.S. and Canadian Coast Guard regulations now require that vessels exchange ballast water at sea before entering the Great Lakes. Since salinity tolerance of the invasive round goby population is poorly understood, we tested 230 laboratory-acclimated fish in three experimental scenarios: (1) rapid salinity increases (0–40 ppt), simulating ballast water exchange, (2) step-wise salinity increases, as during estuarine tidal fluxes or migration from fresh to saltwater, and (3) long-term survivorship and growth (to 4 months) at acclimated salinities. Almost all gobies survived experiments at 0–20 ppt, whereas none survived ≥ 30 ppt, and at 25 ppt only 15% withstood rapid changes and 30% survived step-wise increases. Ventilation frequencies were lowest at 10–15 ppt in step-wise experiments, in conditions that were near isotonic with fish internal plasma concentrations, reflecting lower energy expenditure for osmoregulation. Growth rates appeared greatest at 5–10 ppt, congruent with the larger sizes reached by gobies in Eurasian brackish waters. Thus, we predict that the Great Lakes round goby would thrive in brackish water estuaries along North American coasts, if introduced. However, oceanic salinities appear fatal to the invasive round goby, which likely cannot withstand complete seawater ballast exchanges or oceanic habitats.     

Effectiveness of Piscicides for Controlling Round Gobies (Neogobius melanostomus)

Round gobies (Neogobius melanostomus) were introduced to the Great Lakes presumably as a result of ballast water releases from seagoing freighters returning from European water bodies. These unwelcome fish have become established in the Great Lakes region and are expanding their range to suitable portions of other interior drainage basins including the Mississippi River traversing the central United States and the Trent-Severn waterway spanning south-central Ontario. If the invasion continues, use of chemical toxicants as a control measure may be necessary. Toxicity tests of the currently registered piscicides antimycin, rotenone, 3-trifluoromethyl-4-nitrophenol (TFM), and Bayluscide^® were conducted with three fish species native to the Great Lakes and round gobies collected from the Illinois Waterway. Tests indicated that round gobies are sensitive to all of the piscicides, however, the level of sensitivity is similar to that of the native fish species tested. Therefore, currently registered piscicides have limited potential to selectively remove round gobies. Bottom-release formulations of Bayluscide^® and antimycin were also evaluated as control agents for the normally bottom-dwelling round goby. Avoidance behavior tests demonstrated that the round goby did not react to the presence of either chemical. Therefore, the bottom-release formulations may have some application for the selective removal of round gobies, and may be one of the few tools presently available to fishery managers to help limit the range expansion of this invasive fish.     

Tributary use and large-scale movements of grass carp in Lake Erie

Infrequent captures of invasive, non-native grass carp (Ctenopharyngodon idella) have occurred in Lake Erie over the last 30+ years, with recent evidence suggesting wild reproduction in the lake’s western basin (WB) is occurring. Information on grass carp movements in the Laurentian Great Lakes is lacking, but an improved understanding of large-scale movements and potential areas of aggregation will help inform control strategies and risk assessment if grass carp spread to other parts of Lake Erie and other Great Lakes. Twenty-three grass carp captured in Lake Erie’s WB were implanted with acoustic transmitters and released. Movements were monitored with acoustic receivers deployed throughout Lake Erie and elsewhere in the Great Lakes. Grass carp dispersed up to 236 km, with approximately 25% of fish dispersing greater than 100 km from their release location. Mean daily movements ranged from <0.01 to 2.49 km/day, with the highest daily averages occurring in the spring and summer. The Sandusky, Detroit, and Maumee Rivers, and Plum Creek were the most heavily used WB tributaries. Seventeen percent of grass carp moved into Lake Erie’s central or eastern basins, although all fish eventually returned to the WB. One fish emigrated from Lake Erie through the Huron-Erie Corridor and into Lake Huron. Based on our results, past assessments may have underestimated the potential for grass carp to spread in the Great Lakes. We recommend focusing grass carp control efforts on Sandusky River and Plum Creek given their high use by tagged fish, and secondarily on Maumee and Detroit Rivers.     

Laboratory evaluation of spawning substrate type on potential egg predation by round goby (Neogobius melanostomus)

Spawning habitat structure may protect demersal eggs of broadcast spawning species from depredation. Egg predation by round goby (Neogobius melanostomus) is commonly referenced as a concern associated with their invasion of the Laurentian Great Lakes. Whereas nest-building species have received some attention in egg predation studies, broadcast spawning species may be particularly vulnerable as they do not guard their eggs. This study used a 2?×?7 factorial experiment to investigate how substrate characteristics influenced the rate that fertilized eggs are lost when exposed to round goby. Eggs for two recreationally important broadcast spawning species, northern pike (Esox lucius) and muskellunge (Esox masquinongy), were placed within spawning habitat treatments (bare, silt, sand, rubble, gravel, filamentous algae, and submerged aquatic vegetation) representing a range (least to most complex) of habitat complexity. Regardless of substrate type, egg loss was similar between the two esocid species. Across all substrates and species, the number of eggs lost varied two-fold over the 24-hour experiment, with the lowest rate of egg loss observed in the most complex substrate (submerged aquatic vegetation) and the highest over the least complex substrates (bare and silt). Both northern pike and muskellunge are known to spawn over structurally complex submerged aquatic vegetation and filamentous algae. Although eggs spawned over these substrates likely offer some protection from predation, eggs that settle over less complex ancillary habitats may face higher predation risk.     

Implications of tagging effects for interpreting the performance of sea lamprey traps in a large river

Abundance estimates can be crucial for managing species of economic concern. The accuracy of these estimates can depend on the methods used to track animals and to estimate abundance from tracking data. We tested experimentally if disparate estimates of trapping efficiency calculated for sea lamprey (Petromyzon marinus) in the St. Marys River near Sault Ste. Marie, Canada could be explained by effects related to the invasiveness and handling involved in tagging or the tag size used in the marking procedures. Trapping is used to gauge adult abundance, trapping efficiency, and success of a binational sea lamprey control program in the Laurentian Great Lakes, North America. Our experiment compared nightly catches of sea lamprey marked with external fin clips, surgically-implanted passive integrated transponder tags (PIT-only), and surgically-implanted PIT and acoustic tags (PIT+acoustic). We found no evidence that the probability of being trapped was affected by the added invasiveness and handling of internal tagging. Nightly recaptures of PIT-only tagged sea lamprey, relative to fin-clipped sea lamprey, were not different from expectations based on the numbers of individuals released from each treatment group. Conversely, there was evidence of effects related to tag size. Nightly recaptures of PIT+acoustic tagged sea lamprey, relative to PIT-only tagged sea lamprey, were lower than expected based on numbers of individuals released from each treatment group. Effects related to tag size partially explain the disparate estimates in trapping efficiency observed for sea lamprey.     

Assessing ballast water treatments: Evaluation of viability methods for ambient freshwater microplankton assemblages

For decades the Great Lakes have been subject to invasive species introductions through the discharge of ships' ballast water. Several treatment technologies involving physical, chemical, and biological processes have been developed to remove or inactivate organisms in this discharge. Assessing the efficacy of these technologies involves estimating the number of viable propagules in treated discharge relative to untreated controls. For organisms in the 10–50 µm size range, for example, the International Maritime Organization (IMO) mandates that fewer than 10 viable organisms per milliliter may be discharged. To date, however, there is no standard method to assess viability of natural assemblages of organisms in this size group (largely phytoplankton and protozoans) in freshwater environments. We report here on a process of assemblage concentration, staining with fluorescein diacetate (FDA), and microscopic observation as a reliable and efficient method to assess densities of viable freshwater organisms in this size category in ballast discharge. A number of other methods, including digestion with enzymes, flow cytometry, and a variety of vital and mortal stains, were tested and discarded during this vetting process due to inconsistent or ambiguous results.     

Efficacy of NaCl brine for treatment of ballast water against freshwater invasions

Currently, all transoceanic vessels entering the Great Lakes must perform ballast water exchange or saltwater flushing, procedures designed to reduce the risk of new biological invasions from ballast water. Vessels not in compliance with these regulations presently have limited, often costly, and/or time-consuming alternatives available. Treatment with sodium chloride brine at an initial concentration of 230‰ has been proposed as an emergency ballast water management option and is examined here. Six shipboard trials were conducted under operational conditions to determine the efficacy of brine ballast water treatment. Trials were conducted on three vessels with full ballast tanks and on three vessels with only residual ballast water. Brine distribution in tanks was adequate, noting that vessel movement was essential to ensure mixing into ballast water or ballast residuals. Once mixing has occurred, approximately 25 hour exposure to 45‰ brine or 1 hour exposure to 115‰ brine is required to effectively exterminate freshwater zooplankton. Brine appears to be a cost-effective and relatively safe procedure that could be implemented immediately for emergency treatment of non-compliant ballast water to reduce risk of new invasions in the Great Lakes.     

Dividing the waters: The case for hydrologic separation of the North American Great Lakes and Mississippi River Basins

Legislation has been introduced this year in the U.S. Congress, but not yet enacted, that would direct the U.S. Army Corps of Engineers to complete a study of the options that would prevent the spread of aquatic nuisance species between the Great Lakes and Mississippi River Basins. Hydrologic separation is the only option which closes the aquatic connection between the two basins and does not require continuous operation and maintenance of various technologies that have some risk of failure. The one-time, capital cost to separate the two basins is widely acknowledged to be high, and the outstanding question is whether the costs are justified given the significant risk of future ecological damages and long-term economic losses. Interests opposing separation have mounted a public campaign that the news media have picked up to deny that hydrologic separation should be considered or that a problem even exists. The campaign rests on four assertions: (1) existing electric barriers in the Chicago canals are effective; (2) it is too late--the carps are already in the Great Lakes or soon will be; (3) Asian carps will not thrive in the Great Lakes due to inadequate food and spawning habitat; and (4) Asian carps are unlikely to cause serious harm. Our review of these assertions and the ecological and socio-economic threats to both basins supports our recommendation that the pending legislation be passed and that it include analysis of hydrologic separation of the two basins.     

Long-term evaluation of a sequential batch reactor (SBR) treating dairy wastewater for carbon removal.

Many dairy industries have been using SBR wastewater treatment plants because they allow optimal working condition to be reached. However, to take advantage of SBR capabilities, strong process automation is needed. The aim of this work is to study the factors that influence SBR performance to improve modelling and control. To better understand the whole process we studied the kinetic modelling, the carbon removal mechanism and the relation between reactor performance, aerobic heterotrophic activity and bacterial population dynamics (by terminal restriction fragment length polymorphisms of 16S rDNA, T-RFLP). The heterotrophic activity values presented high variability during some periods; however, this was not reflected on the reactor performance. As sludge health indicator, the average activity in a period was better than individual values. Although all the carbon removal mechanisms are still unclear for this process, they seemed to be influenced by non-respirometric ways (storage, biosorption, accumulation, etc.). The variability of heterotrophic activity could be correlated with the bacterial population diversity over time. Despite the high variability of the activity, a simple kinetic model (pseudo ASM1) based on apparent constant parameters was developed and calibrated. Such modellisation provided a good tool for control purposes.     

Pre-posterior analysis as a tool for data evaluation: Application to aquifer contamination

This paper deals with the frequently encountered problem of pre-posterior data evaluation, i.e., assessment of the value of data before they become available. The role of data is to reduced the risk associated with decisions taken under conditions of uncertainty. However, while the inclusion of relevant data reduces risk, data acquisition involves cost, and there is thus an optimal level beyond which any addition of data has a negative net benefit. The Bayesian approach is applied to construct a method for updating decisions and evaluating the anticipated reduction in risk following consideration of additional data. The methodology is demonstrated on a problem of management of an aquifer under threat of contamination.Notation L matrix of losses for all combinations of states and decisions - l, m, h possible salinity levels from the proposed borehole - N, M, F possible decisions - P(·) vector of prior probabilities of states - P(.|l), P(.|m), P(.|h) conditional (updated) probability vectors of the different states given the salinity levels - P(.|), P(.|), P(.|) probability vectors of the different salinity levels given the true states (likelihood function) - P(l), P(m), P(h) probabilities of the salinity levels, irrespective of the true state - R(.|l), R(.|m), R(.|h) posterior risk vectors of the different decisions given the salinity levels - R(N), R(M), R(F) prior risk associated with different decisions - , , possible true states     

Anaerobic biodegradability of Tween surfactants used as a carbon source for the microbial reductive dechlorination of hexachlorobenzene.

Three structurally-related, nonionic, polysorbate surfactants (Tween 60, 61, and 65) were used as the sole carbon source to sustain the microbial, sequential reductive dechlorination of hexachlorobenzene (HCB) in a mixed, methanogenic culture derived from a contaminated estuarine sediment. The surfactants were partially degraded and fermented to methane with no measurable accumulation of volatile fatty acids, indicating that methanogenesis was rapid relative to the rates of hydrolysis and acidogenesis. Addition of the methanogenesis inhibitor 2-bromoethanesulfonic acid resulted in acetate accumulation without impact on the sequential dechlorination of HCB. An anaerobic biodegradability assay was performed and the following data were obtained for the Tween 60, 61, and 65, respectively: 53, 62, and 62% COD destruction; 35, 57, and 48% COD to methane conversion; and 38, 38, and 45% COD to acetate conversion. These data suggest that the hydrophobic moiety (stearate) of the surfactants was preferentially degraded, most likely through beta-oxidation, to acetate and ultimately to methane and carbon dioxide. Between 38 and 47% of the initial surfactant COD remained after 46 d incubation, which most likely corresponds to the hydrophilic polyoxyethylene moiety. An anaerobic biodegradation pathway of the Tween surfactants is proposed.     

The relevance of species-based indicators as a tool for evaluating the structure of exploited fish assemblages: a comparative study of two tropical lakes in Mali, West Africa

A comparative study of the fish communities in two lake ecosystems in Mali (Lakes Manantali and Selingue) was based on monitoring small‐scale fishery landings for a 13‐month period. The main difference between the two lakes is the fishing pressure, being higher in Lake Selingue and lower in Lake Manantali. The effects of fishing on the structure of the fish communities in the two lakes were analysed with the use of diversity indices, rank species abundance (RSA) and abundance–biomass comparative curve (ABC) methods. The diversity differed significantly between the lakes, being higher for Lake Selingue. Analysis of the community structure, using RSA curves, indicated a more regular distribution of the weights between fish species for Lake Selingue than for Lake Manantali. The two lakes exhibited different exploited fish community structures. For Lake Selingue, the fishery was based on smaller‐sized species, compared to that for Lake Manantali. The indicators based on the species composition of exploited fish communities, in terms of abundance and life‐history traits, seemed to be more relevant in this study. Indicators such as diversity indices and RSA curves depend strongly on the exploitation strategy, being unreliable for evaluating exploited fish communities from catch data.     

Water temperature as a hindrance,but not limiting factor for the survival of warm water invasive crayfish introduced in cold periods

The success of non-native species establishment depends on various abiotic and biotic factors that determine the outcome of an introduction event. Limiting temperature ranges have been studied for various non-native species; however, such previous assessments of species-specific temperature thresholds may be inadequate. Because several non-native crayfish species prefer warmer water temperatures, introductions were generally assumed to occur during preferable, warmer periods. However, despite the generality, traditionally considered ‘warm-water’ species are gradually appearing in new habitats, which were previously considered too cold for successful establishment. Newly discovered overwintering abilities of these species are likely related to the winter stratification in lentic ecosystems, which maintain tolerable conditions. To understand better the survivability of two such non-native species, red swamp crayfish Procambarus clarkii and marbled crayfish Procambarus virginalis individuals were abruptly subjected to a thermic shock which lowered the water temperature from 20 °C (room temperature) to 6 °C, 4 °C and 2 °C, thus mimicking the release by pet owners during various phases of winter. The survival rate and foraging activity were monitored for up to 98 days. Procambarus clarkii showed a considerable higher survival rate at low temperatures (4 °C, 2 °C) compared to that of P. virginalis with neither sex nor size differences evident. Our findings reveal the ability of warm water invaders to withstand a shock during introduction at low temperature periods without acclimation. Considering these newly discovered shifts in physiological limitations, particularly for the red swamp crayfish, this may indicate a higher threat for areas with colder conditions.     

A pilot-plant study of a moving-bed biofilm reactor system using PVA gel as a biocarrier for removals of organic carbon and nitrogen.

A pilot-plant study was conducted to evaluate the performance of a moving-bed biofilm reactor process using PVA-gel beads as a biocarrier. Real primary-settled wastewater was fed to the pre-denitrification system and removals of nitrogenous and organic contaminants were evaluated over a 1-year period. The results demonstrated that at a total nitrogen (TN) loading of 18 mg/L.h, a TN removal efficiency in keeping with and even exceeding the theoretical maximum efficiency based on the level of internal recycle, was possible and a nitrification rate of 15 mg/L.h was sustained with a HRT of only 2.5 h at 15 degrees C. Furthermore, soluble COD and BOD5 in the effluent of the pilot plant were reduced to levels well below most regulatory discharge limits. In addition, the possibility of using this biocarrier in a system, including the elimination of waste organic sludge, was discussed.     

Lakewide dominance does not predict the potential for spread of dreissenids

In recent years, quagga mussels (Dreissena rostriformis bugensis) have almost completely replaced zebra mussels (Dreissena polymorpha) in the Lower Great Lakes. As recreational boats are the main vector of spread for dreissenids in North America, this study examined whether lakes Erie and Ontario could still be sources for the spread of zebra mussels. In the summer–fall of 2010, the abundance of each species of Dreissena on 196 boats from 5 marinas in lakes Erie and Ontario was examined. Additional samples of Dreissena in 2010–2012 were collected in tributaries, bays, and in the upper littoral zones of these lakes. A total of 77 boats were fouled by Dreissena, and of those 61 were fouled by both species, 13 were fouled just by zebra mussels, and only 3 were fouled solely by quagga mussels. Although quagga mussels compose ~ 99% of dreissenids in eastern Lake Erie and in Lake Ontario, on boats at most marinas sampled, zebra mussels were usually more abundant and significantly larger than quagga mussels. Refugia for zebra mussels were found in bays, tributaries, and upper littoral zones with high wave activity. Thus, although quagga mussels are now more abundant than zebra mussels within the Lower Great Lakes, these waterbodies still have the potential to be a source for the spread of zebra mussels, and for some vectors, the propagule pressure from zebra mussels is likely greater than that from quagga mussels.