对话农机手

"三夏"时节,田野麦浪滚滚,收割机机声隆隆。小麦2010年又是丰收年,农民们看着丰收景象,满脸洋溢着喜盈盈的笑,农机手们开着联合收割机,满怀着甜蜜的快乐,机欢唱,人开心,同奏着麦收的"欢乐颂"!     

精致的设计 诗意的栖居——专访青岛绿城建筑设计有限公司暖通主任工程师张小峰

<正>刚出火车站,天空幽蓝,空气湿润,寻路未果,索一路人,依其指点,穿过条条巷道,眼前便是一望无际的大海了,心情一下子就豁然开朗,长途的疲累此时早已     

一种值得称羡的生活方式

正在德国的大街上经常可以看到,既有白发老者,又有高挑美女,还有身着蓝色工装的技工,腋下都夹着一本书,目光祥和,举止优雅,慢悠悠行走着,公交车上、一些乘客静静地读书,有时还能见到在等候乘客的片刻,司机也在翻阅书本。自古以来,读书好学的     

2013 价值远航

时光如水,岁月如歌。《现代零部件》即将走过悠悠十载春秋,在2013年迎来创刊十周年。十年来,《现代零部件》在广大作者和读者的支持与帮助下,脚踏实地,一步一个脚印,取得了令人欣喜的成绩。回首创刊之初,她犹如一株树苗,在春天萌发嫩芽,沐浴阳光雨露,伴随季节更替,历经重重洗礼,     

MATLAB和数控机床在凸轮反求中的应用

文中通过运用三轴数控铣床和寻边器,用极坐标编程,对旋切机的凸轮轮廓进行测点,利用MATLAB对所测数据进行处理,找出轨迹方程,利用CAD/CAM软件的公式曲线等工具建模,最终生产出高品质的产品,不但提高了产品的质量,降低了成本,主要为企业找到了反求的捷径.     

工程机械发展对液压技术的需求动向

进入21世纪以来,世界工程机械发展处于低迷状态,平均增长率有下降趋势,2001年比2000年下降2.2%,自2003年开始,工程机械销售额有一定增长,以日本为例,2003年销售额约100亿美元,比2002年增长15%,其中内需为5247亿日元,增长4%,外需(出口)为6296亿日元,约占总销售额的1/2,增长20%,外需增长主要是中国及亚洲市场需求激增的缘故。由于日本对废气排出量有新的限制,使设备更新量增大,例如,液压挖掘机增长8%,汽车起重机增长12%。据日本建设机械协会预测,2004年日本工程机械将增长9%,达110亿美元,其中液压挖掘机增长16%,小型挖掘机增长6%,由于建设项目…     

开拓创新强化服务大力发展机械工业标准化

中国机械工业联合会是为适应国家工业管理体制改革需要,经国务院同意、民政部批准,于2001年4月正式成立的综合性行业协会。按照国家标准委和国家发展改革委的授权,三年多来,特别是在2004年,我们以科学发展观为指导,以市场需求为导向,以为政府、为企业服务为宗旨,本着不“越位”、不“错位”、不“缺位”的工作原则,围绕振兴机械工业,开拓创新,强化服务,     

专栏征稿函 十载历程 十年成长 喜迎《现代零部件》创刊10周年

2003～2013时光如水,岁月如歌。《现代零部件》即将走过悠悠十载春秋,在2013年迎来创刊十周年。十年来,《现代零部件》在广大作者和读者的支持与帮助下,脚踏实地,一步一个脚印,取得了令人欣喜的成绩。回首创刊之初,她犹如一株树苗,在春天萌发嫩芽,沐浴阳光雨露,伴随季节更替,历经重重洗礼,一路茁壮成长。而今已有沉甸甸的十岁年轮,并在行业媒体中崭露头角。十年历程,您与我们携手并肩一起走     

产能过剩，天胶轮胎利润捆绑下行

天然橡胶、轮胎、汽车,从上到下,从源头到终端,彼此之间关系紧密,价格上有明显的联动性,在利润上也紧密相关,照常理来讲,天然橡胶价格下跌,轮胎的利润在较短时间上升,影响到汽车,也会令汽车行业受益,反之亦然。     

岚县初步实现马铃薯全程机械化生产

岚县位于山西省西北部,吕梁山北端高寒山区,地处黄河流域中部,属晋西北黄土高原山区.辖3镇9乡,面积1 514 km2,境内多为丘陵区,地势较为平坦,平均海拔1200 m以上,平均气温6.8 ℃,有效积温2 727 ℃,日温差14.7 ℃,年平均降雨为504.9 mm,无霜期为90～125 d.独特的自然条件,既是理想的马铃薯生产区域,又适宜机械化生产.全县粮田面积3万hm2,其中马铃薯每年种植面积均在1万hm2以上.占粮田面积的1/3,是农民收入的重要来源之一.实现马铃薯机械化生产具有得天独厚的优势.     

Intravital dual-colored visualization of colorectal liver metastasis in living mice using two photon laser scanning microscopy

A major challenge of cancer biology is to visualize the dynamics of the metastatic process in secondary organs at high optical resolution in vivo real-time. Here, we presented intravital, dual-colored imaging of liver metastasis formation from a single cancer cell to metastatic colonies in the living liver of living mice using two photon laser scanning microscopy (TPLSM). Red fluorescent protein expressing murine (SL4) or human (HT29) colorectal cancer cell lines were inoculated to the spleen of green fluorescent protein expressing mice. Intravital TPLSM was performed by exteriorizing and fixing the liver lobe of living mice. This was repeated several times for the long-term imaging of the same mouse. Viable cancer cells in the living liver of living mice were visualized intravitally at a magnification of over 600×. Single cancer cells were arrested within hepatic sinusoids 2 h after injection. Platelet aggregation surrounding a cancer cell was observed, indicating a phenomenon of tumor-cell induced platelet aggregation. Cancer cells were extravasated from hepatic sinusoids to the space of Disse. Protrusions of Kupffer cells surrounding a cancer cell were observed, indicating that Kupffer cells appear to phagocytose cancer cells. SL4 cells formed liver metastatic colonies with extensive stromal reaction. Liver metastases by HT29 cells were observed as a cluster of micrometastatic nodules. High-resolution, dual-colored, real-time visualization of cancer metastasis using intravital TLPSM can help to understand spatiotemporal tumor-host interactions during metastatic processes in the living organs of living animals.     

Using the atomic force microscope to observe and study the ultrastructure of the living BIU-87 cells of the human bladder cancer

In this study, the ultrastructure of living BIU-87 cells of human bladder cancer was mapped using atomic force microscopy to reveal the dynamic change of single cancerous cell division. Simultaneously, the feasibility and functional reliability of the atomic force microscope (AFM) were established and a laboratory model using AFM to study living cancerous cells was created. In this experiment, BIU-87 cells of human bladder cancer were cultured by conventional methods and grown in gelatin-treated dishes. A thermostat was used for preserving the cell's living temperature. Scanning of these cells using AFM was carried out in physiologic condition. The AFM images of the ultrastructure of living BIU-87 cells as well as those of the cell's membrane and cytoskeleton were very clear. The dynamic phenomenon of single cell division was observed. It was concluded that the AFM was able to observe and depict the ultrastructure of living cells of human bladder cancer directly and in real time. This experimental model is expected to play an important role in elucidating the cancerous mechanism of bladder normal cells at the atomic or nanometer level.     

Comprehensive bioinformatics analysis of CYB561 expression in breast cancer: Link between prognosis and immune infiltration

Background: Cytochrome b561 (CYB561) plays a critical role in neuroendocrine function, cardiovascular regulation, and tumor growth; however, the prognostic value of CYB561 in patients with breast cancer and the relationship between CYB561 expression and immune infiltration in breast cancer remain unclear. Methods: The mRNA expression and clinical data of patients with breast cancer were obtained from The Cancer Genome Atlas database. Functional enrichment analysis was used to explore underlying biological functions associated with CYB561. The methylation status of CYB561 was analyzed using the MethSurv database. The enrichment score of immune cell infiltration for CYB561 in breast cancer was calculated using single-sample gene set enrichment analysis. The prognostic value of CYB561 was evaluated using Kaplan-Meier method and Cox regression analysis. Based on the results of the multivariate Cox analysis, a nomogram was constructed to predict the effect of CYB561 expression on overall survival (OS). Results: The results showed that CYB561 was highly expressed in breast cancer tissues. Hypomethylation of CYB561 is associated with an unfavorable prognosis. In multivariate Cox regression analysis, CYB561 was an independent prognostic factor for OS. Functional enrichment analysis indicated that estrogen signaling pathway, inflammatory response, KRAS signaling pathway, epithelial-mesenchymal transition, leukocyte migration, and regulation of lymphocyte activation were strongly enriched in the low CYB561 expression group. Additionally, CYB561 expression was negatively correlated with immune infiltration of B cells, plasmacytoid dendritic cells, dendritic cells, and neutrophils. Conclusion: CYB561 may serve as a potential biomarker for breast cancer diagnosis and prognosis.     

Curcumin gum Arabic nanoparticles demonstrate potent antioxidant and cytotoxic properties in human cancer cells

The main purpose of the study was to enhance the stability and therapeutic effects of Curcumin (Cur) through nanoformulation with gum Arabic (GA) as a coating agent through an efficient synthetic approach. The antioxidant properties of the developed nanoparticles (Cur/GANPs) were assessed through several in vitro assays, such as β-carotene bleaching activity, DPPH, and nitric oxide scavenging activities in addition to evaluating its inhibitory activity on angiotensin-converting enzyme (ACE). The cytotoxicity of Cur/GANPs was evaluated in vitro using different types of human cancer cells including breast cancer (MCF7, MDA-MB231), liver cancer (HepG2), and colon cancer (HT29) cells. The prepared particles displayed an elliptical shape with a size ranging between 20–260 nm and a potential difference of –15 mV. The Cur/GANPs exhibited significant antioxidant activity compared to free curcumin when using concentrations between 31.5 and 500 µg/mL. The Cur/GANPs also had inhibited the growth of all cancer cell lines in a proportional trend with concentrations used. Hence, the encapsulation with gum Arabic has augmented the antioxidant and anti-neoplastic effects of Curcumin. Therefore, Cur/GANPs may have effective therapeutic properties in diseases attributed to oxidative stress like cancer and hypertension.     

Effects of polydatin on the proliferation,migration, and invasion of ovarian cancer

To investigate the effects of polydatin on the proliferation, migration, and invasion of ovarian cancer, the change of proliferative ability, migration ability, and invasive ability of human ovarian cancer cell OVCAR-3, A2780, and HO-8910 was detected by using polydatin and up-regulating PI3K. The anticancer activity and mechanism of polydatin in ovarian cancer were analyzed. Polydatin could effectively inhibit the proliferation, migration, and invasion of OVCAR-3, A2780, and HO-8910, and inhibit the expression of PI3K protein. After the expression level of PI3K protein was up-regulated, the inhibitory effect of polydatin on the proliferative ability, migration ability, and invasive ability of OVCAR-3, A2780, and HO-8910 significantly decreased, suggesting that PI3K was the target of polydatin. Therefore, we concluded that polydatin could inhibit the proliferation, migration, and invasion of ovarian cancer cells by inhibiting the expression of PI3K protein, which provides an experimental basis for polydatin in the treatment of ovarian cancer.     

Synchrotron nanoscopy imaging study of scalp hair in breast cancer patients and healthy individuals: Difference in medulla loss and cortical membrane enhancements

Nanoscopic synchrotron X‐ray imaging was performed on scalp hair samples of patients with breast cancer and healthy individuals to investigate any structural differences as diagnostic tool. Hair strands were divided into 2‐3 segments along the strands from root to tip, followed by imaging either in projection or in CT scanning with a monochromatic 6.78‐keV X‐ray using zone‐plate optics with a resolving power of 60 nm. All the examined cancer hairs exhibited medulla loss with cancer stage‐dependent pattern; complete loss, discontinuous or trace along the strands. In contrast, medullas were well retained without complete loss in the healthy hair. In the CT‐scanned axial images, the cortical spindle compartments had no contrast in the healthy hair, but appeared hypointense in contrast to the surrounding hyperintense cortical membrane complex in the cancer hair. In conclusion, observation of medulla loss and cortical membrane enhancements in the hair strands of breast cancer patients demonstrated structural variations in the cancer hair, providing a new platform for further synchrotron X‐ray imaging study of screening breast cancer patients. Microsc. Res. Tech. 79:23–30, 2016. © 2015 Wiley Periodicals, Inc.     

图像处理和特征分析在评估乳腺癌近期发病风险中的应用

图像处理和特征分析在乳腺癌早期诊断中发挥着重要作用。为了将一个基于左右乳腺X光片图像密度特征不对称性的图像特征分析方法应用于乳腺癌近期发病风险的预测,训练开发了一个以多图像特征相融合的人工智能分辨模型来预测每个妇女在近期(比如,在最近一次的良性检测以后2年之内)由医学影像检测出来的早期癌症的风险程度。介绍这一个新癌症风险预测模型的基本原理,演示一个新型的人机交互式的计算机辅助图像特征分析和计算的工作平台。初步实验证明,应用这一个基于医学图像特征定量分析的模型能够取得比采用现行其他已知的乳腺癌风险因子有着显著提高的对于近期乳腺癌发病风险的预测精度,这将为建立更有效的个性化乳腺癌普查提供依据和帮助。     

Biomarker discovery in lung cancer--promises and challenges of clinical proteomics

Lung cancer is a devastating illness with an overall poor prognosis. To effectively address this disease, early detection and novel therapeutics are required. Early detection of lung cancer is challenging, in part because of the lack of adequate tumor biomarkers. The goal of this review is to summarize the knowledge of current biomarkers in lung cancer, with a focus on important serum biomarkers. The current knowledge on the known serum cytokines and tumor biomarkers of lung cancer will be presented. Emerging trends and new findings in the search for novel diagnostic and therapeutic tumor biomarkers using proteomics technologies and platforms are emphasized, including recent advances in mass spectrometry to facilitate tumor biomarker discovery program in lung cancer. It is our hope that validation of these new research platforms and technologies will result in improved early detection, prognostication, and finally the treatment of lung cancer with potential novel molecularly targeted therapeutics.     

Identification of a 10-pseudogenes signature as a novel prognosis biomarker for ovarian cancer

The outcomes of ovarian cancer are complicated and usually unfavorable due to their diagnoses at a late stage. Identifying the efficient prognostic biomarkers to improve the survival of ovarian cancer is urgently warranted. The survival-related pseudogenes retrieved from the Cancer Genome Atlas database were screened by univariate Cox regression analysis and further assessed by least absolute shrinkage and selection operator (LASSO) method. A risk score model based on the prognostic pseudogenes was also constructed. The pseudogene-mRNA regulatory networks were established using correlation analysis, and their potent roles in the ovarian cancer progression were uncovered by functional enrichment analysis. Lastly, ssGSEA and ESTIMATE algorithms was used to evaluate the levels of immune cell infiltrations in cancer tissues and explore their relationship with risk signature. A prediction model of 10-pseudogenes including RPL10P6, AC026688.1, FAR2P4, AL391840.2, AC068647.2, FAM35BP, GBP1P1, ARL4AP5, RPS3AP2, and AMD1P1 was established. The 10-pseudogenes signature was demonstrated to be an independent prognostic factor in patient with ovarian cancer in the random set (hazard ratio [HR] = 2.512, 95% confidence interval [CI] = 2.03–3.11, P < 0.001) and total set (HR = 1.71, 95% CI = 1.472–1.988, P < 0.001). When models integrating with age, grade, stage, and risk signature, the Area Under Curve (AUC) of the 1-year, 3-year, 5-year and 10-year Receiver Operating Characteristic curve in the random set and total set were 0.854, 0.824, 0.855, 0.805 and 0.679, 0.697, 0.739, 0.790, respectively. The results of functional enrichment analysis indicated that the underlying mechanisms by which these pseudogenes influence cancer prognosis may involve the immune-related biological processes and signaling pathways. Correlation analysis showed that risk signature was significantly correlated with immune cell infiltration and immune score. We identified a novel 10-pseudogenes signature to predict the survival of patients with ovarian cancer, and that may serve as novel possible prognostic biomarkers and therapeutic targets for ovarian cancer.