高效液相色谱法测定乳粉中维生素C

本文建立了一种高效液相色谱法测定乳粉中维生素C含量的方法。采用0.5%的草酸提取液对乳粉中维生素C进行提取,采用C18色谱柱,以磷酸缓冲盐为流动相,流速0.7ml/min,紫外检测器,检测波长266nm,维生素C质量浓度在1-250mg/L范围内线性关系良好,加标回收率为95.4%-99.5%。此方法操作简单,快速,准确,简化了样品前处理过程,可用于乳粉中维生素C的测定。     

高效液相色谱法测定苋菜等有色样品的维生素C

本研究用HPLC法测定苋菜中的维生素C,色谱条件为Nova-pak C_(18)柱(3.9×300mm),0.1％草酸流动相,紫外检测器(254nm)。该方法不受植物色素及还原剂的影响,为测定有色蔬菜样品的理想方法。对61个苋菜品种进行取样测定,结果显示,苋菜维生素C的含量范围是34～81mg/100g鲜重,其中最高达81.37mg/100g鲜重。     

高效液相色谱法测定牛肉中三聚氰胺的残留量

建立了一种测定牛肉中三聚氰胺的残留量的高效液相色谱法。样品中加入三氯乙酸作为提取溶剂,乙酸铅沉淀蛋白,过混合阳离子交换柱净化,残留物用流动相定容,高效液相色谱二极管检测器测定,外标法定量。结果表明,三聚氰胺的检出限为0．2mg／kg,平均回收率在72％～85％之间,相对标准偏差小于5％。     

HPLC法测定箭红气雾剂中维生素C的研究

箭红气雾剂系我单位研制的军用特药(待报批),主要用于火箭发射现场,推进剂四氧化二氮及其有害产物,吸入中毒的急救与防治。经药理试验证明,箭红气雾剂可有效防治四氧化二氮及其有害产物吸入中毒导致的喉头、气管、支气管、肺泡、淋巴管及血管等化学损伤,减轻或解除化学性肺炎引起的呼吸系统渗出增加、支气管痉挛、肺水肿、呼吸功能障碍症状。箭红气雾剂由维生素Ｃ等4种有效成份经气雾制剂工艺加工制成。新药研究中,对箭红气雾剂中4种有效成份的含量测定方法进行了研究。维生素Ｃ系药典(1995年版二部)收载品种,采用碘量法测定含量。本文研究…     

直接皂化-高效液相色谱法测定奶粉中维生素A和E

目的:建立用高效液相色谱法同时测定奶粉中维生素A、E含量的方法。方法:奶粉样品经过皂化处理后,直接取上清液进样到高效液相色谱仪中,紫外双波长检测。结果:维生素A、E的浓度线性范围分别为0.25～1.25μg/mL、2.50～12.50μg/mL,最低检出浓度分别为0.511μg/100g、13.3μg/100g;相对标准偏差为1.0%~5.7%,回收率为98.0%~102.2%。结论:该法简单、快速、准确,满足检测需要,适合奶粉中维生素A和E的检测。     

高效液相色谱法测定水果中的抗坏血酸

用高效液相色谱法测定了几种水果中的维生素C,并研究了不同的色谱条件、不同的提取液对测定结果的影响,结果表明用0.25%的偏磷酸浸提样品以及作为流动相效果较好,测得Vc的保留时间为4.35,回收率为92%～98%,线性范围0.0001～1.68mg/ml.     

维酶素中辅酶和VB2的液相色谱分析

维酶素作为营养保健食品填加剂，对萎缩性胃炎和食道上皮细胞增胜有显著疗效。本文采用高压液相色谱法对纤维素中维生素Ｂ２和两种辅酶（ＦＭＮ和ＦＡＤ）进行了分析。     

功能因子Melatonin(褪黑素)的HPLC分析检测方法研究

Melatonin(褪黑素)以其独特的昼夜排泌节律日益引起人们的重视,本文根据melatonin的特点建立了高效快捷的HPLC分析检测方法,此方法具有样品处理简单、快速、定量准确和重复性好等特点。其CV％值分别为1.46 ％和2.03％。     

高效液相色谱法检测小麦粉中的偶氮甲酰胺方法的研究

小麦粉中偶氮甲酰胺(azodicardonamide,ADA)用丙酮提取,提取液氮吹浓缩后用5%二甲基亚矾的水溶液定容,并用正己烷脱脂。样品供高效液相色谱仪测定,外标法定量。方法线性范围是0～100mg/L(r~2=0.9979),定量下限为4mg/kg。在小麦粉基质粉中分别添加10.0mg/kg、50.0mg/kg两个水平的偶氮甲酰胺标准品,方法的回收率为84%～90%,相对标准偏差小于5%。     

正相高效液相色谱法检测火箭煤油中的芳烃

在ASTM6379标准方法的基础上,建立火箭煤油中芳烃的液相色谱-紫外检测方法。该方法采用氨基柱串联氰基柱,以正己烷为流动相,流速1.0mL/min,柱温28℃。单环芳烃以邻二甲苯计在8~80mg/L（r2=0.9999）范围内线形关系良好,定量限0.008mg/L,在已知浓度的样品溶液中分别加入高、中、低3种不同浓度的标准品溶液,回收率85.4%~114.1%,相对标准偏差小于5%。该方法简便易行、准确可靠。     

Determination of benzimidazoles in pharmaceuticals and human serum by high-performance liquid chromatography

A liquid chromatographic method is described for the quantification of prednisolone, benzimidazoles, and preservatives using a C₁₈ analytical column as stationary phase. The mobile phase was 30:70 methanol:pH 2.5 phosphate buffer at a flow rate of 1.0 mL min^?1 with absorbance detection at 235 nm. The method was linear for concentrations ranged from 40–10,000 ng mL^?1. Low values of coefficient of variance were obtained when samples were analyzed as replicates. Excellent recovery values were recorded in commercial products and fortified samples. International Conference of Harmonization protocols were employed to perform comprehensive method validation. The reported method has applications for pharmaceutical and serum samples.     

Determination of propyl gallate in edible oil by high-performance liquid chromatography with chemiluminescence detection

A novel and convenient method based on high-performance liquid chromatography coupled with chemiluminescence (HPLC) for the determination of propyl gallate in edible oil is reported. The detection was based on the inhibition of chemiluminescence between luminol and hydrogen peroxide in alkaline solution. The analysis was performed using a C₁₈ column with isocratic 60:40 methanol/water. Under the optimum conditions, the concentration of propyl gallate was linear from 9?×?10^?6?mol?L^?1 to 1?×?10^?4?mol?L^?1 with a detection limit of 2?×?10^?7?mol?L^?1. The method is simple, sensitive, and inexpensive and was used for the determination of propyl gallate in edible oil.     

Determination of baicalin in cosmetics and toothpaste by solid-phase extraction and high-performance liquid chromatography

Baicalin was determined in cosmetics and toothpaste by high-performance liquid chromatography. The samples were extracted with ultrasound in 75% ethanol for 20 min, purified by anion exchange solid-phase extraction cartridge, and the components were separated on a C18 column. The limits of detection and quantification were 3.0 mg kg^?1 and 10.0 mg kg^?1. The recovery was from 81.3–101.6%. A linear relationship was present from 1.00–100 µg · mL^?1 of baicalin. The protocol was simple, sensitive, selective, and used to determine baicalin in personal care products. Baicalin was not present in materials contrary to the labeled contents.     

液相色谱法测定滴鼻液中羟甲唑啉含量

通过方法学研究,建立羟甲唑啉滴鼻液含量测定的高效液相色谱法。线性范围为2~12mg,r=0.9996;平均回收率100.3％。相对标准偏差1.24％。     

Determination of methyl gallate in Bauhinia retusa extract by high-performance liquid and thin-layer chromatography

Two stability-indicating chromatographic methods are reported for the determination of methyl gallate in crude extracts of Bauhinia retusa. Separation by high performance thin layer chromatography was conducted on silica gel aluminum sheets using 9.5:0.5:0.2 (v/v/v) chloroform:methanol:acetic acid at 280 nm. The results from the 2–40 µg/band were used to prepare a linear calibration graph. The limits of detection and quantitation were 0.5 and 1.5 µg/band, respectively. The reverse phase high performance liquid chromatographic isolation of methyl gallate was performed at ambient temperature with an injection volume of 10 μL. The mobile phase consisted of 40:60 (v/v) methanol:0.1% ortho-phosphoric acid. The separation was performed at 1 mL/min using a detection wavelength of 280 nm. The calibration graph for methyl gallate was rectilinear from 0.02–40 µg/mL with limits of detection and quantitation of 0.004 and 0.010 µg/mL, respectively. For both methods, intra-day and inter-day precision were evaluated and the relative standard deviation was less than 2%, indicating good precision. The robustness was evaluated by making small and deliberate changes to appropriate parameters and the calculated relative standard deviation was less than 2%.The chromatographic methods were employed to determine methyl gallate in crude Bauhinia retusa extracts.     

高效液相色谱法测定大鼠全血中的灵芝酸单体Me

建立大鼠全血中灵芝酸单体Me的高效液相色谱测定方法。采用色谱柱Diamonsil~(?)C_(18)柱(250mm×4.6μm,5μm),流动相为甲醇-水(体积比为94:6,用醋酸调pH至3.6);流速为1mL/min;检测波长为245nm;内标为苯丙酸诺龙。灵芝酸单体Me血中浓度在0.05～40μg/mL范围内线性良好(r>0.99),定量限为50ng/mL,日内和日间测定的精密度(以相对标准偏差表示)均低于8.6%。该方法简便、灵敏、准确,适用于大鼠体内灵芝酸的药物动力学研究。