不同来源胶原肽对光老化皮肤成纤维细胞的影响研究

比较研究了海蜇、牛骨和鳕鱼皮3种不同来源胶原肽对光老化皮肤成纤维细胞的修复作用。发现3种胶原肽均可显著提高光老化细胞的细胞增殖活性,经紫外照射后,人皮肤成纤维细胞Ⅰ型、Ⅲ型胶原蛋白及透明质酸合成量明显降低,表现为差异性极显著(P0.01),同时细胞内抗氧化酶系T-SOD、CAT和GSH-Px的抗氧化酶活力也都降低。当加入3种胶原肽后,光老化细胞的Ⅰ型、Ⅲ型胶原蛋白及透明质酸合成量与细胞内T-SOD、CAT和GSH-Px酶活力均有所增加,且改善光老化细胞内抗氧化酶活力与增加细胞胶原分泌效果较好的是海蜇胶原肽。     

海洋胶原蛋白肽在功能性食品中的应用

本文综述了海洋胶原蛋白肽在功能性食品领域的应用情况。介绍了海洋胶原蛋白的结构特点,论述了海洋胶原蛋白肽的制备技术,重点探讨了海洋胶原蛋白肽的生物学功能,包括延缓皮肤衰老、改善骨骼健康、调节血压、保护胃粘膜和抗氧化性等生物学功能,最后对海洋胶原蛋白肽在功能性食品领域的应用前景进行了展望。     

胶原和水解胶原蛋白的结构、性能和功效研究及应用前景

胶原蛋白是一类在所有哺乳动物中都存在的纤维状、大分子蛋白质,普遍分布于细胞外基质(ECM)中。它是动物体内细胞间隙中最重要,也是含量最多、最丰富的蛋白质,起着支撑器官保护机体的功能。胶原蛋白所持有的许多优良特性使其在各领域中的应用具有极大的潜质。水解胶原蛋白由于交联结构的解体和分子量大大降低使其溶解性和口服吸收利用率大大的提高。此外,胶原多肽还可以促进食品中的其它蛋白质的吸收。本综述对胶原及水解胶原蛋白的组成、结构、功效、应用和开发前景进行了研究和探讨。     

胶原、明胶和水解胶原蛋白的护肤功能比较

通过乌氏黏度法测定胶原、明胶和水解胶原蛋白的相对黏度和特性黏数,三硝基苯磺酸(TNBS)法测定样品的自由氨基质量摩尔浓度,在不同相对湿度环境下测定样品的吸湿性和保湿性,荧光标记样品方法观察它们在大白鼠皮肤表层的分布和渗透情况以及角质形成细胞培养实验评价3种样品的生物活性,研究了3种样品的物理化学性能和护肤功能差异。结果表明:胶原的相对黏度是明胶和水解胶原蛋白的5倍左右,其特性黏数也明显大于明胶和水解胶原蛋白;胶原的自由氨基质量摩尔浓度最小,其次是明胶,水解胶原蛋白最大;胶原的吸湿性优于明胶和水解胶原蛋白;胶原、明胶和水解胶原蛋白都能渗透至毛囊中,但只有胶原仍均匀分布于皮肤表面,显示其优良的保湿功能;只有胶原能明显促进细胞的生长和繁殖,而在明胶和水解胶原蛋白上培养的角质形成细胞的生长情况与参比样相似。因此,胶原比明胶和水解胶原蛋白具有更优良的吸湿、保湿性能以及抗皮肤衰老功效。     

牛骨胶原蛋白肽促进人成骨细胞增殖

目的研究牛骨胶原蛋白肽对人成骨细胞增殖的影响。方法分离培养人成骨细胞,茜素红矿化染色鉴定人成骨细胞的成骨特性,利用MTT实验检测胶原蛋白肽对人成骨细胞的增殖效应。结果茜素红矿化染色结果表明,HBC具有成骨能力,胶原蛋白肽对HBC的增殖具有促进作用,0.3mg/ml胶原蛋白肽对细胞的增殖具有最佳效应。结论牛骨胶原蛋白肽促进人成骨细胞增殖,为骨质疏松的预防和治疗提供理论依据。     

鱼皮胶原蛋白膜的研究进展

国内对鱼皮再利用的研究主要集中在鱼皮胶原蛋白的提取和胶原蛋白肽的制备。随着研究的进一步深入,以鱼皮胶原蛋白制膜的研究日渐活跃。本文综述了鱼皮胶原蛋白膜的制备工艺和改性方法,以及其在食品、医药、生物制品等领域的应用,并对鱼皮胶原蛋白膜的综合开发应用进行了展望。     

1～100kDalton的胶原水解物的分子量分布测定的研究

采用凝胶板配方和电泳条件优化后的SDS-PAGE技术对酸法明胶水解物、碱法明胶水解物、胃蛋白酶法明胶水解物、胃蛋白酶法猪皮胶原水解物、明胶以及猪皮胶原蛋白透析物等进行表征,获得了将分布于一块凝胶板上的1～100kD的棒状胶原蛋白各组分的色谱图。胃蛋白酶法猪皮胶原蛋白水解物经电泳分离后的色谱图中,各谱带清晰明显,分子量均一,且均匀分布于1～100kD不等,这对棒状胶原蛋白分子的研究具有重要价值。     

明胶文摘

<正>水解胶原蛋白摄入对人体血浆中羟脯氨酸游离肽和多肽剂量变化的影响Shigemura Y,et al.Food chemistry,2014,159:328-332.人们普遍认为摄入胶原水解物后,人体血浆中出现的含羟脯氨酸的多肽是对身体健康有益的。为了评估这种的多肽的有效含量,我们研究了摄人胶原水解物总量和消化后血浆中的多肽含量的关系。4名志愿者分别摄人每千克体重30.8,153.8,384.6mg的胶原水解物。     

复合多肽延缓小鼠皮肤衰老的功效研究

通过细胞增殖、抗氧化、抗炎以及促进胶原蛋白4个细胞实验研究了棕榈酰三肽-5,五肽-3,大豆多肽,类蛇毒肽和六肽-11 5种多肽的抗衰能力和复合后的效果提升作用,采用UVB联合D-半乳糖皮下注射造模方式研究了5种多肽复合后的皮肤抗衰老作用。结果表明,5种多肽具有一定的促细胞增殖、抗氧化、抗炎及促胶原蛋白合成能力,且5种多肽复合后具有显著的效果提升作用,复合多肽能够显著改善和缓解UVB联合D-半乳糖皮下注射引起的小鼠皮肤表皮增厚、胶原较少、弹性蛋白减少情况,并且能够显著抑制造模引起的IL-1,IL-6,TNF-α表达上调,抑制MMP3,MMP9,β-半乳糖苷酶的表达。以上结果表明5种多肽复合能够通过缓解UVB联合D-半乳糖皮下注射造模造成的表皮增厚、炎症、细胞外基质流失及衰老相关酶表达增加来发挥皮肤抗衰老作用。     

水产胶原蛋白及其生物活性肽的研究和应用

介绍了胶原蛋白的基本理化特性、制备方法和利用途径,阐述了水产胶原蛋白和陆生哺乳动物胶原蛋白的区别,列举了水产胶原蛋白、胶原蛋白肽制备的基本方法和其生物活性,指出水产胶原蛋白和胶原蛋白肽有着广阔的利用前景。     

Application Properties of Bath Liquids for Children Based on Sodium Laureth Sulfate with Addition of Different Molecular Weight Collagens Derived from Marine Sources

This article investigates the influence of marine collagen of various molecular weights (Collagen Amino Acids—150 Da, Hydrolyzed Collagen—12,000 Da and Soluble Collagen—300,000 Da) on the functional properties of bath liquids for children based on anionic surfactants—sodium laureth sulfate. In addition to the aspects related to safety-in-use, which were presented in the authors' first work about marine collagen, consumers also expect bath cosmetics for children to have specific functional characteristics including viscosity or foaming properties. An increase in the molecular mass of marine collagen was found to be accompanied by an increase in dynamic viscosity and foaming ability and a decrease in the ability to emulsify fatty soil in the formulations under study. It can, therefore, be concluded that the addition of the highest molecular weight of marine collagen (300,000 Da) to bath liquids for children contributes not only to reducing the irritant effect caused by anionic surfactants, but also improves the usable properties of these types of cosmetics.     

脂质体包裹胶原蛋白的制备及其透皮性能

为提高胶原蛋白体外透皮吸收性能,以卵磷脂和胆固醇为成膜材料,采用薄膜分散法制备了鱼皮水解胶原蛋白脂质体。以模拟Franze扩散池进行小鼠离体皮肤渗透扩散实验,采用高效液相色谱法(high performance liquid chromatography,HPLC)测定胶原蛋白含量,考察胶原蛋白脂质体的透皮吸收量与吸收率。实验结果表明,相对分子质量(简称分子量,下同)3 500的鱼皮水解胶原蛋白经脂质体包封后,透皮吸收量在24 h内达到21.9%,较直接涂抹胶原蛋白的透皮吸收率提高一倍。证明小分子胶原蛋白经脂质体包封能够改善其体外透皮吸收性能。     

氨基化改性胶原蛋白的研究进展

胶原蛋白是生物体内重要的组织蛋白,来源广泛,种类繁多,具有良好的生物相容性、可生物降解性以及生物活性等,是一种重要的功能性材料。然而未改性的胶原蛋白理化性质较差,如热稳定性、机械强度及耐酶性等,限制了胶原蛋白的应用范围。因此,如何改性胶原蛋白,从而提高其理化性质一直是研究者们关注的热点。氨基作为胶原蛋白侧链上的主要活性基团之一,是胶原蛋白表面正电荷的主要来源,在与其他物质的交联中发挥不可或缺的作用。因此,氨基化改性胶原蛋白可以增加胶原蛋白上的活性位点,提高其表面电荷,有利于拓宽其应用范围。在此背景下,该文主要介绍了胶原蛋白的理化性质和主要的改性方法,综述了氨基化胶原蛋白在生物医学、环境治理及皮革工业等方面的最新研究进展,并对未来氨基化胶原蛋白在皮革清洁无铬鞣制系统中的应用前景进行了展望。     

Effects of static axial strain on the tensile properties and failure mechanisms of self-assembled collagen fibers

Collagen fibers form the structural units of connective tissue throughout the body, transmitting force, maintaining shape, and providing a scaffold for cells. Our laboratory has studied collagen self-assembly since the 1970s. In this study, collagen fibers were self-assembled from molecular collagen solutions and then stretched to enhance alignment. Fibers were tested in uniaxial tension to study the mechanical properties and failure mechanisms. Results reported suggest that axial orientation of collagen fibrils can be achieved by stretching uncrosslinked collagen fibers. Stretching by about 30% not only results in decreased diameter and increased tensile strength but also leads to unusual failure mechanisms that inhibit crack propagation across the fiber. It is proposed that stretching serves to generate oriented fibrillar substructure in self-assembled collagen fibers. © 1997 John Wiley & Sons, Inc. J Appl Polym Sci 63: 1429–1440, 1997     

基于胶原纤维的吸附分离材料研究进展

家畜动物的皮主要被用于生产皮革,构成动物皮的主要成分是胶原纤维,它是一种天然高分子材料,具有独特的物理化学性质。近年来人们利用胶原纤维研究制备了多种功能材料。本文系统地介绍了将胶原纤维用于制备天然产物、重金属离子、无机阴离子、蛋白质和微生物的吸附分离材料方面的研究工作以及这些吸附分离材料的应用原理和应用性能,为环境保护、天然产物纯化、蛋白质分离等领域提供了可借鉴的技术手段。同时,这些研究工作表明,资源丰富的皮胶原纤维不仅是某些传统产业的加工原料,也是创制先进功能材料的基础物质,胶原纤维的开发利用有值得想象的广阔空间。     

Characterization and implantation of a novel foamy type of collagen into SD rats to regenerate tissue by slowing down the collagen degradation rate

Collagen has high biocompatibility and biodegradability and therefore is an ideal natural polymer biomaterial for tissue regeneration, such as gel-like and porous collagens. However, the limitation of gel-like collagen is unsuitability for cell/tissue ingrowth and the limitation of porous collagen is quick degradation rate. Here, the authors propose a novel type of foamy collagen to address the previous limitations. Foamy collagen with a closed/nonconnective porous structure was formed using foaming technology and not using toxic crosslinking reagents. This research aimed to investigate the macro-/microstructure, the in vitro/vivo degradation rate, and the tissue regeneration feasibility of foamy collagen. For in vitro degradation rate, porous collagen was completely degraded by enzyme, whereas 91.5% and 72.1% of gel-like and foamy collagens, respectively, remained intact. In vivo degradation rate had a similar trend as in vitro data. After implantation of the collagens in Sprague Dawley rats, immune cells were observed at the periphery of the three types of collagen at day 3. Fibroblast ingrowth was observed in foamy and porous collagen groups at day 7. Neocapillary formation and tissue regeneration were observed in foamy and porous collagen groups at day 14, but nearly none in gel-like collagen group. In conclusion, the authors believe that foamy collagen is promising for application of soft-tissue regeneration.     

Effect of crosslinking method on collagen fiber-fibroblast interactions

Collagen, the major structural protein of the extracellular matrix in animals, is a versatile biomaterial used in various tissue engineering applications. Cross-linking influences the mechanical properties, resorption kinetics, and biocompatibility of collagen-based biomaterials. In this study, we evaluated the effects of crosslinking on collagen fiber-fibroblast interactions in vitro. Collagen fibers were left untreated or crosslinked by ultraviolet (UV) irradiation, dehydrothermal (DHT) treatment (3 or 5 days), or hexamethylenediisocyanate (HMDIC) exposure. The initial attachment, proliferation (through 8 days), and morphology of human dermal fibroblasts were evaluated on control and crosslinked bundles of 200 collagen fibers in vitro. Initial attachment (number of fibroblasts at day 0) was increased on UV and DHT5-treated collagen fiber bundles. Fibroblast proliferation was similar for control, UV crosslinked, and DHT crosslinked fibers. In contrast, fibroblast attachment was significantly decreased and proliferation was delayed on HMDIC crosslinked fibers. These results, coupled with our previous studies, suggest that UV crosslinking of collagen fibers provides a combination of biocompatibility, mechanical properties, and strength retention suitable for various tissue engineering applications. © 1997 John Wiley & Sons, Inc. J Appl Polym Sci 63: 1493–1498, 1997     

绿色资源——胶原合成复合材料的研究

胶原是绿色资源之一,胶原与其它高分子化合物复合可以形成新的复合材料。复合材料性能好,应用范围广。本文对绿色资源胶原合成复合材料的研究进行综述。