使用光纤实现荧光定量PCR检测

阐述了光纤在荧光定量PCR检测中的应用特点和荧光产生机理,给出了光纤耦合效率的计算公式。介绍了试验装置的构成和工作原理,列举了限制荧光检测的实际问题并提供了消除模块背景的方法。试剂检测的结果证实了使用光纤的检测系统具有很高的检测分辨率和8个数量级以上的动态线性范围,完全满足荧光定量PCR检测的要求。     

NO光纤传感器研究进展

一氧化氮(NO)是生物体内一种重要的信使分子和效应分子,在生理和病理过程中起着重要作用,实现对生物体内NO快速、准确的检测,具有重要的意义,将光纤传感技术应用到对NO的检测中是目前最有前景的方法之一。本文综述了国内外NO光纤传感器的研究进展,介绍了几种适用于光纤传感器的新型NO探针和检测方法,并对NO光纤传感技术目前存在的问题及发展趋势进行了讨论。     

用光纤传感器阵列测量动态变量

用光纤传感器阵列测量动态变量Ｊ．Ｎｉｅｗｉｓｃｈ等１．概述分布式光纤传感器或由光纤连接成的光传感器阵列，在大面积、多测量点的监视方面有很大的潜力。定位分辨率可用光时域反射计（ＯＴＤＲ）的原理来表达，这个方法最典型的用途为静态效应（仅随时间作慢变化的测...     

质量控制和检测用传感器的技术动向及光纤传感器

质量控制和检测用传感器的技术动向及光纤传感器服部肇一、前言一个企业的活力在于确实把握用户的需求并据此制造出优良的产品的能力，因此质量控制的情况在很大程度上可反映企业的兴旺程度。随着需求进一步向高级化、个别化、差别化发展，生产也出现了多品种、少数量的趋...     

测量表面粗糙度的新型光纤传感器

介绍了一种测量表面粗糙度的准直型光纤传感器。该传感器成功地消除了光纤测头到被测表面距离变动和环境的杂散光线对输出的影响,传感器光通量的计算方法可用于位移及其它反射型光纤传感器的特性分析和结构设计。     

F-P光纤应力传感器研制

分析了F-P光纤应力传感器工作原理,提出了F-P光纤应力传感器的数学模型,设计了F-P光纤应力传感器弹性体结构、光电转换系统.F-P光纤应力传感器光源电路设计采用APC电路.APC电路由两个运算放大器和晶体管以及外围电路组成,采用背向光反馈自动偏置控制方式,即用半导体激光器组件中的PD光电二极管监测LD背向输出的光功率.对F-P光纤应力传感器进行了静态特性标定试验,试验结果表明,该传感器具有较高的测量精度,能够满足工程测量要求.     

共轭聚合物荧光传感器

共轭聚合物由于存在分子线效应,比传统荧光小分子相比,作为传感器具有更高的灵敏度。本文介绍了共轭聚合物荧光信号放大机理,并举例说明了几种常见了的共轭聚合物荧光传感器的应用。     

蓝宝石光纤与光纤高温传感器

蓝宝石光纤作为一种高品质的单晶光纤,具有极好的高温稳定性和光学、机械特性,因此在光纤高温传感和中红外激光传能方面具有相当重要的应用价值。 浙江大学物理系光纤电子材料研究室单晶光纤及应用研究课题组。于1987年在国内首次拉制成功蓝宝石单晶光纤和YAG单晶光纤,并经过多年努力,在蓝宝石光纤高温传感头及光纤测温仪的实用化研究中,取得了较好的研究结果。目前已建立起稳定的晶体光纤激光加热基座法(LHPG)生长设备;能小批量生长优质的蓝宝石单晶光纤材料,光纤光传输损耗优于     

溶胶-凝胶法制备基于荧光猝灭原理的光纤氧传感器在线监测水中溶解氧

利用氧分子对金属钌化合物的荧光具有猝灭作用的特性,构造出基于荧光猝灭原理的光纤氧传感器。就用于水中溶解氧在线监测的光纤光谱仪的搭建、传感器探头的设制及传感膜的制作过程进行探索,并对传感器的响应性能进行考察。结果表明以联吡啶钌等作为荧光指示剂以溶胶-凝胶法制备的传感膜对溶解氧的响应具有良好的可逆性,稳定性,较快的响应时间和较长的使用寿命。与标准法相对照,用本仪器系统测定了不同盐度的人工海水中的溶解氧浓度,两种方法在不同浓度水平下的溶解氧测定值均无显著性差异。本法的日内和日间RSD在1.7％～5.0％之间。     

A multi-purpose reflective fiber optic sensor

Fiber optic sensors, including reflective ones, have widespread applications in sensing technology. In this study, we first formulated the gain of a general reflective fiber optic sensor in terms of five major sensor parameters, and for two patterns of input intensity: namely uniform and Gaussian. By using these formulae, we have analyzed and plotted the variations of gain versus one or two of these parameters. The results obtained have exact compatibility for the two patterns of input intensity, although they have been obtained by different methods. The results can be suitably used in the optimum design of a wide variety of reflective fiber optic sensors.     

Single-mode D-shaped optical fiber sensor for the refractive index monitoring of liquid

A new fabrication method is introduced for the production of D-shaped optical fiber. A mechanical end and edge polishing system with aluminum oxide polishing film is utilized to perform sequential polishing on one side (lengthwise) of single-mode optical fiber in order to obtain a D-shaped cross section. Adjusting specific mechanical parameters allows for control of the volume of the D-shaped zone, while the fiber surface smoothness is governed by selection of polishing film grit size. To meet the accuracy and repeatability requirements, optical power loss is monitored during the entire polishing process in situ and in real time. This proposed technique possesses advantages of rapidity, safety, simplicity, repeatability and stability with high precision in comparison with contemporary methods for production. Sensor performance tests on the fiber reveal a linear response with linearity up to R² = 0.984 for surrounding refractive index in the range of 1.320–1.342 refractive index, which corresponds to different concentrations of the glucose solution test environment. The produced D-shaped optical fiber has potential sensing and monitoring applications in chemical, environmental, biological and biochemical fields.     

A dual modality optical fiber sensor

We propose and demonstrate a fibre optic system based on bi-tapered silica fibre that can simultaneously measure strain and fibre curvature. Both modalities on the signal can be extracted with no measurable crosstalk between them. The experimental signal has a pure phase modulation when strain is applied to the tapered fibre optic section of the sensor and the signal shows only intensity modulation when an un-tapered fibre section is bent. High sensitivity is achieved from the experimental results for strain and bending losses and the estimation of measurement errors is 0.2 and 0.1%, respectively. This system offers low-cost, compactness and it can be adapted for structural health monitoring.     

Study of a fiber optic humidity sensor based on agarose gel

An optical fiber humidity sensor was fabricated using a hydrophilic gel (agarose) deposited on the tapered plastic optical fiber (POF). The sensing element, agarose, can absorb and exude moisture from/to the ambience, thereby altering its refractive index and changing its ability to modulate the intensity of light that propagates through the fiber. Thus, the operating principle of the sensor is based on the intensity modulation technique, which utilizes a tapered POF probe coated with agarose that is sensitive to humidity. The POF, which was fabricated using an etching method, has a waist diameter of 0.45 mm and tapering length of 10 mm. As the relative humidity varies from 50% to 80%, the output voltage of the sensor with agarose gel of 0.5% weight content decreases linearly from 2.24 mV to 1.55 mV. The agarose-based sensor produces a sensitivity of 0.0228 mV/%, with a slope linearity of more than 98.36%. The tapered fiber with agarose gel of 1% weight content produces a sensitivity of 0.0103 mV/% with a slope linearity of more than 94.95% and a limit of detection of 2.635%, while the tapered fiber with agarose gel of 1.5% weight content produces a sensitivity of 0.0079 mV/% with a slope linearity of more than 98.53% and a limit of detection of 6.853%. The fiber with agarose gel of 0.5% weight content shows higher sensitivity compared to that of 1% and 1.5% due to the effect of pore size, which changes with concentration. The results demonstrate that agarose-based optical fiber sensors are both sensitive and efficient for economical and flexible measurements of humidity.     

SQL server的分布式光纤温度传感器校准数据采集方法

利用Lab VIEW数据采集系统平台,提出了一种采用SQL server技术访问InterBase数据库的数据采集方法.该方法先将分布式光纤温度传感器自带的GDB格式数据,以数据表的形式存入SQL Server特定数据库中,然后通过Lab VIEW数据采集系统以数组形式显示,最后将数组数据保存在Excel文件中,以便数据分析处理.运行调试结果表明,该方法对被校分布式光纤温度传感器的校准数据采集保存快速,操作分析方便,可视化效果好.     

Noise properties of a bidirectional optical fiber transmission system using Raman amplifers

Abstract

Noise properties of a bidirectional optical fiber transmission system using Raman amplification are analyzed. Analytical expressions for both forward and backward amplified spontaneous emission (ASE) noise are established in terms of system parameters. The signal to ASE noise ratio (SNR) and the achievable repeater length are discussed. Based on the results, we find that there exist optimum pump and initial power levels for the maximum SNR and repeater spacing. The maximum system transmission length and accumulated ASE noise for a multiple‐amplifier system are calculated. A maximum system transmission length up to 5,000 km with 12 dB SNR is then predicted.     

聚合物光纤网络器件及其通信链路系统

通过自行研制的聚合物光纤波长转换器和集线器,实现了石英光纤传输介质到聚合物光纤传输介质的工作波长转换,以及多路聚合物光纤传输信息的交换和局域网信息的全聚合物光纤传输。系统测试结果显示,数据交换传输速率达到100Mbps,通信眼图清晰。链路系统传输信号随传输距离成指数衰减,与聚合物光纤的光强衰减规律一致。用商用的发射接收器测得传输距离达到60m以上。聚合物光纤弯曲半径为25mm时,给系统接收端接收信号强度带来0.1~0.4dB的衰减,表明聚合物光纤弯曲对链路系统工作影响较小。     

光纤出射光强分布研究

对多模光纤出射光束的光强分布用横向位移法进行了理论分析和实验研究,并用数值计算的方法分析实验测量数据.结果表明,高斯光束可以很好地描述光纤出射光强的分布,光纤出射光束光强分布参数主要与光纤芯径和出纤光功率等有关,为强度调制型光纤传感器的优化研制提供了必要的实验依据.     

光谱吸收式光纤甲烷气体传感器及其信号处理方法

基于气体在其特征吸收波长下光的吸收随浓度变化的机理,通过对甲烷气体吸收光谱的分析,提出了一种光谱吸收式全光纤甲烷气体传感器及其系统。该系统以1300nm波段的DFBLD作为光源,波长响应范围为1100~1900nm的高灵敏度、低噪声的InGaAsPIN作为光电探测器,利用锁相放大器对传输信号进行相敏检波,采用一次谐波反馈光源注入电流、二次谐波与一次谐波的比值作为系统输出的谐波检测技术对微弱信号进行处理,使得系统达到很高的灵敏度。研究表明,该传感器的灵敏度达到10ppm,精确度和稳定性等性能指标均可满足甲烷气体检测要求。