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1.
Maternal dietary conjugated linoleic acid alters hepatic triacylglycerol and tissue fatty acids in hatched chicks 总被引:1,自引:0,他引:1
The effects of feeding CLA to hens on newly hatched chick hepatic and carcass lipid content, liver TAG accumulation, and FA
incorporation in chick tissues such as liver, heart, brain, and adipose were studied. These tissues were selected owing to
their respective roles in lipid assimilation (liver), as a major oxidation site (heart), as a site enriched with long-chain
polyunsaturates for function (brain), and as a storage depot (adipose). Eggs with no, low, or high levels of CLA were produced
by feeding hens a corn-soybean meal-basal diet containing 3% (w/w) corn oil (Control), 2.5% corn oil +0.5% CLA oil (CLA1),
or 2% corn oil +1.0% CLA oil (CLA2). The egg yolk content of total CLA was 0.0, 1.0, and 2.6% for Control, CLA1, and CLA2,
respectively (P<0.05). Maternal dietary CLA resulted in a decrease in chick carcass total fat (P<0.05). Liver tissue of CLA2 chicks had the lowest fat content (P<0.05). The liver TAG content was 8.2, 5.8, and 5.1 mg/g for Control, CLA1, and CLA2 chicks, respectively (P<0.05). The chicks hatched from CLA1 and CLA2 incorporated higher levels of cis-9,trans-11 CLA in the liver, plasma, adipose, and brain than Control (P<0.05). The content of 18∶0 was higher in the liver, plasma adipose, and brain of CLA1 and CLA2 than Control (P<0.05), but no difference was observed in the 18∶0 content of heart tissue. A significant reduction in 18∶1 was observed in
the liver, plasma, adipose, heart, and brain of CLA1 and CLA2 chicks (P<0.05). DHA (22∶6n−3) was reduced in the heart and brain of CLA1 and CLA2 chicks (P<0.05). No difference was observed in carcass weight, dry matter, or ash content of chicks (P>0.05). The hatchabilities of fertile eggs were 78, 34, and 38% for Control, CLA1, and CLA2, respectively (P<0.05). The early dead chicks were higher in CLA1 and CLA2 than Control (18 and 32% compared with 9% for Control), and alive
but not hatched chicks were 15 and 19% for CLA1 and CLA2, compared with 8% for Control (P<0.05). Maternal supplementation with CLA leads to a reduction in hatchability, liver TAG, and carcass total fat in newly
hatched chicks. 相似文献
2.
The aim of the present study was to investigate the effect of cis-9, trans-11 and trans-10, cis-12 CLA on FA composition of TAG in epididymal adipose tissue and liver, and of hepatic phospholipids PL. Twenty-four Syrian
Golden hamsters were randomly divided into three groups of eight animals each and fed semipurified atherogenic diets supplemented
with either 0.5 g/100g diet of linoleic acid or cis-9, trans-11 or trans-12, cis-9 CLA for 6 wk. Total lipids were extracted, and TAG and PL were separated by TLC. FA profile in lipid species from liver
and adipose tissue, as well as in feces, was determined by GC. Trans-10, cis-12 CLA feeding significantly reduced linoleic and linolenic acids in TAG from both tissues, leading to reduced total PUFA
content. Moreover, in the epididymal adipose tissue docosenoic and arachidonic acids were significantly increased. In liver
PL, although no changes in individual FA were observed, total saturated FA (SFA) were decreased. No changes in TAG and PL
FA profiles were induced by the cis-9, trans-11 CLA. TAG and PL incorporated cis-9, trans-11 more readily than trans-11, cis-12 CLA. This difference was not due to differential intestinal absorption, as shown by the analysis of feces. We concluded
that only trans-10, cis-12 CLA induces changes in FA composition. Whereas increased PUFA content was observed in either liver or adipose tissue TAG,
decreased SFA were found in liver PL. Incorporation of cis-9, trans-11 CLA in TAG is greater than that of trans-10, cis-12 CLA, but this is not due to differences in intestinal absorption. 相似文献
3.
Hepatic lipid characteristics and histopathology of laying hens fed CLA or n−3 fatty acids 总被引:2,自引:0,他引:2
The effect of dietary CLA and n−3 PUFA on hepatic TAG accumulation, histopathology, and FA incorporation in lipid classes
by laying chickens was investigated. One hundred twenty 30-wk-old single-comb white leghorn laying hens were distributed randomly
to four treatments (3 replications of 10 birds) and were fed diets containing CLA and animal fat (Diet I), 18∶3n−3 (Diet II),
or long-chain n−3 FA (Diet III). A sunflower oil (n−6 FA)-based diet was the control. Feeding Diet I resulted in an increase
in hepatic total lipids (P<0.05). The liver TAG content was 32.2, 18.9, 29.4, and 18.7 mg/g for hens fed Diet I, Diet II, Diet III, and the control
diet, respectively (P<0.05). The serum TAG was lowest in bilds fed Diet II (P<0.05). Diet I resulted in an increase in the total number of fat vacuoles and lipid infiltration in hepatocytes (P<0.05). The number of cells with 75% or higher lipid vacuolation was observed only in birds fed Diet I. Feeding diets containing
CLA resulted in an increase in the content of the c9,t11 CLA isomer in liver TAG and PC (P<0.05). No difference was observed in the CLA concentration of hepatic PE fractions. The content of DHA (22∶6n−3) was higher
in the TAG, PC, and PE of hens fed Diet II and Diet III than Diet I and the control (P<0.05). Feeding CLA resulted in an increase in total saturated FA in the TAG and PC fractions (P<0.05). Long-term feeding of CLA in laying birds leads to an increase in liver TAG and may predispose birds to fatty liver
hemorrhagic syndrome. 相似文献
4.
The effect of dietary CLA along with n-3 PUFA on yolk FA profile and hepatic lipid accumulation was investigated. Laying hens
(n=40) were randomly assigned to four experimental diets containing 0, 0.5, 1.0, or 2.0% CLA. Menhaden oil was used as the source
of n-3 PUFA. Dietary CLA did not affect the total lipid content of egg yolk (P>0.05). The amounts of CLA isomers (cis-9 trans-11, trans-10 cis-12) in the egg yolk were proportional to the levels of CLA in the diet (P<0.05). The total CLA content in the egg yolk was 0, 0.97, 2.4, and 5.3 wt%, respectively (P<0.05). Addition of CLA resulted in an increase in saturated FA (P<0.05) with a concomitant reduction in monounsaturated FA (P<0.05) in the yolk, liver, abdominal fat, breast, and thigh muscle. No difference in saturated and monounsaturated FA content
in heart and spleen tissue was noted. Dietary CLA at all concentrations resulted in an increase (P<0.05) in the total number of fat vacuoles and lipid infiltration in hepatocytes. The number of cells with 75% or higher lipid
vacuolation in the cytoplasm was also increased (P<0.05) by 2.0% CLA. Dietary CLA at 0.5% levels resulted in an increase (P<0.05) in the total lipid content of hepatic tissue. The total lipid content in leg muscle was lower (P<0.05) in CLA-fed birds. However, no effect of CLA on lipid content of breast muscle, heart, spleen and adipose tissue was
observed (P>0.05). The current study used CLA in a FFA form. The effects of using CLA in other form such as TG on avian hepatic tissue
need to be investigated. 相似文献
5.
It has been reported that consumption of CLA and EPA alters lipid metabolism. CLA contains conjugated double bonds, and EPA
is an n−3 PUFA. Based on the possibility that a molecule with both of these structures might have interesting physiological
effects, we prepared conjugated FA from EPA by alkaline isomerization and examined the effects of the conjugated EPA (CEPA)
on lipid metabolism in rats. Rats were fed by oral gavage every day for 4 wk with 200 mg of FA including linoleic acid, EPA,
CLA, or CEPA. Compared with other groups, rats fed CEPA showed a significant weight loss in epididymal adipose tissue and
significant decreases in the levels of liver TAG and total cholesterol (TC), indicating reduced accumulation of lipid in the
liver and adipose tissue. The plasma levels of TAG, TC, FFA, and tumor necrosis factor-α in rats fed CEPA were reduced, as
was the activity of the FA synthesis system in the liver, whereas the FA-β-oxidation system was activated by CEPA. These results
suggest that intake of CEPA suppresses lipid accumulation in the liver and epididymal adipose tissue while increasing lipid
catabolism in rats. 相似文献
6.
The content and positional distribution of CLA in TAG fractions of lamb tissues was examined with either preformed CLA or
the linoleic acid precursor of CLA in the diet as experimental treatments. The CLA content of phospholipid (PL) from these
tissues was also examined. Thirteen lambs were randomized to the following dietary treatments: (i) control diet (no supplement);
(ii) CLA supplementation (0.33 g d−1 for 21 d prior to weaning) to milk-replacer of pre-ruminating lambs, or (iii) feeding linoleic acid-rich oil (6% safflower
oil on a dry matter basis) to weaned ruminating lambs. At slaughter, tissue samples were procured from diaphragm, rib muscle,
and subcutaneous (SC) adipose tissue. Safflower oil supplementation in the diet resulted in an increase in CLA content of
the TAG from diaphragm, rib muscle, and SC adipose tissue by about threefold (P<0.05) on a mol% basis. CLA was localized to the sn-1/3 positions of TAG. Animals that received pre-formed CLA, however, had increased proportions of CLA at the sn-2 position of TAG from SC adipose tissue, suggesting that there were tissue-specific dietary effects and possible age-related
effects on the mode of FA incorporation into TAG. Safflower oil supplementation in the diet had no effect on the CLA content
of PL from diaphragm, rib muscle, and SC adipose tissue, suggesting that CLA was preferentially incorporated into the TAG
of these tissues. 相似文献
7.
CLA has been shown to induce or suppress excess liver lipid accumulation in various animal models. Interestingly, the state
of insulin resistance may be an important modulator of this effect. The objective of the current study was to determine how
feeding a dietary CLA mixture would affect liver lipid accumulation in insulin-resistant/obese and lean rats in relation to
liver function, lipidemia, liver TAG and phospholipid FA composition, and expression of hepatic markers of FA transport, oxidation,
and synthesis. Six-week-old fa/fa and lean Zucker rats (n=20/genotype) were fed either a 1.5% CLA mixture or a control diet for 8 wk. CLA supplementation reduced liver lipid concentration
of fa/fa rats by 62% in concurrence with improved liver function (lower serum alanine aminotransferase and alkaline phosphatase) and
favorable modification of the serum lipoprotein profile (reduced VLDL and LDL and elevated HDL) compared with control-fed
fa/fa rats. The fa/fa genotype had two-thirds the amount of CLA (as % total FA) incorporated into liver TAG and phospholipids compared with the
lean genotype. In both genotypes, CLA altered the hepatic FA profile (TAG greater than phospholipids) and these changes were
explained by a desaturase enzyme index. Liver-FA-binding protein and acyl CoA oxidase, markers of FA transport and oxidation,
respectively, were expressed at higher levels in CLA-fed fa/fa rats. In summary, these results illustrate a strong relationship between the state of insulin resistance and liver lipid
metabolism and suggest that CLA acts to favorably modify lipid metabolism in fa/fa Zucker rats. 相似文献
8.
Dietary CLA has been shown to enhance glucose tolerance in several animal models, but in mice it induces insulin resistance
and lipodystrophy. In this study, the effects of 2 wk of diet supplementation with either 1,5% CLA or 0.2% troglitazone (TZD),
an insulin-sensitizing thiazolidinedione, on glucose tolerance, lipid accumulation, and composition of both lean and Zucker
diabetic fatty (fa/fa; ZDF) rats were examined. Compared with lean rats, which maintained normal glucose tolerances after 2 wk of feeding regardless
of diet, ZDF rats fed a control diet (CON) had significantly worsened glucose tolerance. ZDF rats fed CLA and TZD diets, however,
maintained normal glucose tolerances. In contrast to the significantly elevated lipid levels in ZDF rats fed the CON diet,
concentrations of plasma FFA and TG in ZDF rats fed CLA and TZD diets were normalized. A similar reduction of plasma lipid
levels was observed in lean rats fed CLA and TZD compared with lean rats fed the CON diet. Although ZDF CON rats developed
significant hepatic steatosis, both CLA-and TZD-fed rats had hepatic TG levels similar to those of lean rats. Both lean and
ZDF rats fed the CLA diet had reduced adipose mass compared with respective genotype controls; however, TZD had no effect.
Ratios of 16∶1/16∶0 and 18∶1/18∶0 FA, surrogate markers for stearoyl-CoA desaturase-1 (SCD-1) activity, were reduced in livers
of ZDF rats fed CLA and TZD diets. These results show that, like TZD, CLA normalizes glucose tolerance and plasma lipids and
also improves hepatic steatosis and FA composition in ZDF rats. The effects of CLA and TZD on hepatic lipid composition suggest
that the effects of these two agents on glucose tolerance may be associated with a reduction in SCD-1. 相似文献
9.
Skuladottir GV Olason PI Jonsson L Skarphedinsson JO Eriksdottir VH Atlason PT Franzson L Schiöth HB 《Lipids》2003,38(11):1127-1132
Hyperphagia was achieved by continuous intracerebroventricular infusion of a melanocortin receptor antagonist (HS024; Neosystem,
Strasbourg, France) in rats. The effects of hyperphagia on FA composition and concentration of plasma phospholipids (PL),
plasma FFA, and adipose tissue TAG were studied in rats for 8 d [short-term hyperphagia (STH); n=8], or 28 d [longterm hyperphagia (LTH); n=9]. The control rats were treated with artificial cerebrospinal fluid for 8 d (n=8) or 28 d (n=10). The rats were fed the same regular diet. In STH rats the plasma PL and fasting plasma FFA contained higher concentrations
of saturated FA (SFA) and monounsaturated FA (MUFA), and plasma FFA contained lower n−6 PUFA than in the control rats. In
LTH rats the plasma PL contained higher concentrations of SFA, MUFA, and n−3 PUFA and higher proportions of 16∶1n−7 and 18∶1n−9
at the expense of 18∶2n−6 than in the control rats. In LTH rats the abundant dietary intake of 18∶2n−6 did not enrich 18∶2n−6
of the plasma PL or adipose tissue TAG. In LTH rats the fasting plasma FFA contained more than twofold higher concentrations
of SFA and MUFA, and higher proportions of 16∶1n−7 and 18∶1n−9 at the expense of 18∶2n−6 than in the control rats. This animal
obesity model shows that LTH affects the FA composition and concentration of plasma PL, plasma FFA, and adipose tissue TAG,
a result consistent with changes associated with increased risk of various diseases in humans. These results also demonstrate
that LTH alters the FA composition of plasma PL and adipose tissue TAG in a way that does not reflect the FA composition of
dietary fat. 相似文献
10.
Mice fed diets containing conjugated linoleic acid (CLA) are leaner than mice not fed CLA. This anti-obesity effect is amplified
in mice fed coconut oil-containing or fat free diets, compared to soy oil diets. The present objective was to determine if
CLA alters lipolysis in mice fed different base oils. Mice were fed diets containing soy oil (SO), coconut oil (CO), or fat
free (FF) for 6 weeks, followed by 10 or 12 days of CLA or no CLA supplementation. Body fat, tissue weights, and ex vivo lipolysis
were determined. Relative protein abundance and activation of perilipin, hormone sensitive lipase (HSL), adipose triglyceride
lipase (ATGL), and adipose differentiation related protein (ADRP) were determined by western blotting. CLA feeding caused
mice to have less (P < 0.05) body fat than non-CLA fed mice. This was enhanced in CO and FF-fed mice (CLA × oil source, P < 0.05). There was also a CLA × oil source interaction on lipolysis as CO + CLA and FF + CLA-fed mice had increased (P < 0.05) rates of lipolysis but SO + CLA-fed mice did not. However, after 12 days of CLA consumption, activated perilipin
was increased (P < 0.05) only in SO + CLA-fed mice and total HSL and ATGL were decreased (P < 0.05) in CO + CLA-fed mice. Therefore, the enhanced CLA-induced body fat loss in CO and FF-fed mice appears to involve
increased lipolysis but this effect may be decreasing by 12 days of CLA consumption. 相似文献
11.
Dietary conjugated linolenic acid in relation to CLA differently modifies body fat mass and serum and liver lipid levels in rats 总被引:6,自引:0,他引:6
Koba K Akahoshi A Yamasaki M Tanaka K Yamada K Iwata T Kamegai T Tsutsumi K Sugano M 《Lipids》2002,37(4):343-350
The present study compared the effect of dietary conjugated linolenic acid (CLNA) on body fat and serum and liver lipid levels
with that of CLA in rats. FFA rich in linoleic acid, α-linolenic acid, CLA, or CLNA were used as experimental fats. Male Sprague-Dawley
rats (4 wk old) were fed purified diets containing 1% of one of these experimental fats. After 4 wk of feeding, adipose tissue
weights, serum and liver lipid concentrations, serum tumor necrosis factor (TNF)-α and leptin levels, and hepatic β-oxidation
activities were measured. Compared with linoleic acid, CLA and, more potently, CLNA were found to reduce perirenal adipose
tissue weight. The same trend was observed in the weight of epididymal adipose tissue. CLNA, but not CLA, was found to significantly
increase serum and liver IG concentrations. Serum FFA concentration was also increased in the CLNA group more than in the
other groups. The activity of β-oxidation in liver mitochondria and peroxisomes was significantly higher in the CLNA group
than in the other groups. Thus, the amount of liver TG exceeded the ability of hepatic β-oxidation. Significant positive correlation
was found between the adipose tissue weights and serum leptin levels in all animals (vs. perirenal: r=0.557, P<0.001; vs. epididymal: r=0.405, P<0.05). A less significant correlation was found between adipose tissue weights and serum TNF-α level (vs. perirenal: r=0.069, P<0.1; vs. epididymal: r=0.382, P<0.05). Although the mechanism for the specific effect of CLNA is not clear at present, these findings indicate that in rats
CLNA modulated the body fat and TG metabolism differently from CLA. 相似文献
12.
Gaofeng Yuan Haiyan Sun Andrew J. Sinclair Duo Li 《European Journal of Lipid Science and Technology》2009,111(6):537-545
The effects of two isomers of conjugated linolenic acid (CLnA), α‐eleostearic acid (α‐ESA) and punicic acid (PA), on body fat and lipid metabolism were investigated, compared with a conjugated linoleic acid (CLA) mixture (primarily cis9,trans11‐ and trans10,cis12‐18:2) and α‐linolenic acid (ALA), a non‐conjugated octadecatrienoic acid, in the present study. ICR mice were fed either a control diet or one of four experimental diets supplemented with 1% α‐ESA, 1% PA, 1% CLA mixture and 1% ALA in the form of triacylglycerols (TAG) for 6 weeks. The weights of perirenal and epididymal adipose tissues were significantly decreased while the liver weight was significantly increased in mice fed CLA, compared with the control. In contrast to CLA, the tissue weights in α—ESA‐, PA‐ and ALA‐fed mice were not affected. No significant differences were observed in TAG, total cholesterol, high‐density lipoprotein and low‐density lipoprotein cholesterol levels among the five groups. The liver TAG level was significantly decreased in mice fed α‐ESA and PA while it was significantly increased in mice fed the CLA mixture. These results indicate that CLnA and CLA have differential effects on body fat mass and liver TAG levels in mice. 相似文献
13.
Conjugated linoleic acid (CLA) reduces body weight and adipose mass in a variety of species. The mechanisms by which CLA depletes
adipose mass are unclear, but two independent microarray analyses indicate that in white adipose tissue (WAT), uncoupling
protein 1 (UCP1) was among genes most changed by CLA. The objective of this study was to determine whether CLA induces ectopic
expression of UCP1 in WAT, which may contribute to increased energy expenditure and weight loss. Six-week old, male ob/ob mice were fed either a control diet (CON) or a diet supplemented with 1.5% mixed isomer CLA (CLA) for 4 weeks. A third group
of mice (LEPTIN) was fed the control diet and received daily injections of recombinant leptin as a positive control for adipose
depletion in ob/ob mice. CLA did not alter several mRNA markers of lipid oxidation in epididymal white adipose tissue (eWAT) , but significantly
increased carnitine palmitoyltransferase-1b (CPT1b) and PPAR gamma coactivator-1α (PGC1α) expression. Notably, CLA increased
both mRNA and protein expression of uncoupling protein-1 (UCP1). β3-adrenoceptor mRNA and phosphorylated-p38 mitogen activated
protein kinase (MAPK) protein levels were not affected by CLA, but were upregulated by LEPTIN. These data suggest the increased
CPT1b, PGC1α, and UCP1, in WAT of CLA-fed mice may contribute to the depletion of adipose, and CLA does not appear to increase
UCP1 through β3-adrenergic signaling. Future studies will focus on understanding how CLA increases mitochondrial oxidation
and energy dissipation in white adipose tissue. 相似文献
14.
The inhibitory effects of 1,3-diacylglycerol (DAG) on diet-induced lipid accumulation in liver and abdominal adipose tissue
of rats were investigated in the present study. Male Sprague-Dawley rats were given free access to diets containing 7 wt%
TAG (low TAG), 20 wt% TAG (high TAG), or 20 wt% DAG (high DAG), respectively, for 8 wk. The body weight of rats in the 20%
high-TAG group increased significantly, and the weights of their abdominal adipose tissue and liver also showed a significant
increase compared with rats in the low-TAG group. However, the high-DAG diet resulted in both a significant reduction in body
weight gain (with a decrease of 70.5%) and an increase in the ratio of abdominal fat to body weight (by 127%) compared with
the high-TAG diet. As well, the liver TAG and serum TAG levels of the high-DAG group were significantly lower than those of
the high-IAG group. These effects were associated with up-regulation of acyl-CoA carnitine acyltransferase (ACAT) and down-regulation
of acyl-CoA DAG acyltransferase (DGAT) in the liver. However, no significant difference was observed in the activities of
alanine aminotransferase and aspartate aminotransferase among the groups (P>0.05). The present results indicate that dietary DAG reduced fat accumulation in viscera and body, and these effects may
be involved with up-regulation of ACAT and down-regulation of DGAT in the liver. 相似文献
15.
Lipase-catalyzed acidolysis of different TAG with CLA was performed to produce structured lipids (SL) containing CLA. An immobilized
lipase from Mucor miehei (Lipozyme IM, Novo Nordisk Inc., Franklinton, NC) was used as the biocatalyst in a solvent-free system. Conconut oil and
tricaprylin, which are sources of medium-chain FA, were the starting substrates, and a mixture of FFA (MFFA) containing 73%
CLA was the donor of the acyl groups. For each TAG, four different ratios of TAG/MFFA were blended to prepare about 500 g
of mixture containing 10, 20, 30, and 40% CLA (w/w). Each blend was reacted with 5% lipase at 65°C for 48 h under nitrogen.
Over the range of TAG/MFFA ratios examined, CLA was incorporated effectively by the enzyme. Lipozyme IM exhibited no special
preference for any particular FA, since the incorporation of FA was proportional to their concentration in the system. FFA,
PV, p-anisidine value (p-AV), iodine value (IV), and saponification number (SN) were evaluated for all the SL. FFA, PV, and p-AV depended on the purification process and showed no significant deterioration of SL with respect to the original TAG, whereas
IV and SN depended on the composition of the SL, mainly the CLA content. 相似文献
16.
trans-10, cis-12-CLA (t10,c12-CLA) inhibits lipid deposition in adipose tissue of many species, but it also enhances lipid deposition in liver. We evaluated
effects of dietary t10,c12-CLA content and gender on carcass composition, FA profile of selected tissues, and expression of FA synthase (FAS) and
stearoyl-CoA desaturase-1 (SCD) mRNA in adipose tissue. Male and female (63 of each) CD-1 mice were assigned a diet containing
0.0, 0.15, or 0.30% t10,c12-CLA at 4 wk of age. Seven mice per dietary group within gender were sacrificed after 2,4 or 6 wk. The CLA isomer caused
dose-dependent reductions in dry carcass weight and fat content, without altering protein content, but carcass fat and epididymal
fat pad weights of males were reduced to a greater extent than carcass fat and inguinal fat pad weights of females. FAS and
SCD mRNA in adipose tissue was more abundant in females than males, but expression in both genders decreased as the t10, c12-CLA content of the diet increased. Although the weight of gastrocnemius muscle was not influenced by diet, total FA content
of the muscle of both genders decreased in response to dietary t10,c12-CLA content. Femur weight of male mice increased as the t10,c12-CLA content of the diet increased, but the weight increase was associated with a reduction in total FA content. The Δ9
desaturation indices for muscle and femur suggested a linear reduction in SCD activity, whereas Δ9 indices for liver indicated
linear enhancement of SCD activity. Overall, results suggested that growing male mice were more susceptible than females to
t10,c12-CLA inhibition of lipid deposition. 相似文献
17.
Gaofeng Yuan Mengmeng Xu Meijuan Tan Jian Dong Xiaoe Chen 《European Journal of Lipid Science and Technology》2021,123(1):2000224
The study aims to investigate the effect of combined supplementation with docosahexaenoic acid (C22:6 n-3, DHA) and vitamin E (VE) on the oxidative stress and liver triglycerides (TG) accumulation induced by high-fat diet (HFD) in mice. C57BL/6J mice are fed either a control diet or an HFD for 8 weeks. Animals are supplemented with DHA, VE, or DHA + VE, respectively. Supplementation with DHA alone shows significant improvement in oxidative stress and hepatic steatosis in mice. Supplementation with DHA significantly reduces the liver TG and total cholesterol contents, and the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, compared with the HFD. Supplementation with DHA also significantly decreases the mRNA expression level of sterol regulatory element-binding protein 1C. However, supplementation with VE alone does not show improvement in oxidative stress and hepatic steatosis. DHA + VE supply obtains a superior effect in alleviation of hepatic steatosis than DHA supplementation alone in mice fed by HFD. The efficacy of DHA potentiated by VE can be due to that VE enhances the effect of DHA in decrease of ALT and AST levels and increase of antioxidant enzyme activity and glutathione level in mice fed by HFD. Practical Applications: Supplementation with DHA significantly improves the oxidative stress and hepatic steatosis induced by HFD in mice. The efficacy of DHA in the alleviation of hepatic steatosis induced by HFD is potentiated by VE. These findings may provide a rational basis for the use of DHA and VE co-supplementation in patients with liver steatosis. 相似文献
18.
Jesper Lundbom Marjukka Kolehmainen Leena Pulkkinen Pasi Soininen Mika Tiainen Ursula Schwab Matti Uusitupa Markku Tammi 《European Journal of Lipid Science and Technology》2010,112(12):1308-1314
The aim of this study was to evaluate use of infrared spectroscopy for measuring adipose tissue triacylglycerols (TAGs) with analysis by multivariate curve resolution (MCR). The mid‐infrared spectrum was measured with an attenuated total reflection accessory from a lipid droplet pressed from adipose tissue. The obtained spectra were characteristic of pure TAG spectra and water and protein contamination could be easily identified from specific spectral regions. MCR analysis of the olefinic (?C? H) stretch (3006 cm?1), resolved the different contributions of monounsaturated (MUFA) and polyunsaturated (PUFA) double bonds. Similar MCR analysis of the trans (HC?CH? ) region (966 cm?1), resolved the differing contributions of isolated trans isomers (transFA) and CLA. The PUFA double bond content of 16 subjects was negatively correlated with concentrations of serum total cholesterol R = ?0.498 (p = 0.050) and triacylglycerols R = ?0.609, (p = 0.016). The transFA content exhibited a negative, although non‐significant, correlation to high‐density lipoprotein (HDL)‐cholesterol (R = ?0.483, p = 0.068). The present study shows that MCR analysis of adipose tissue TAG infrared spectra can be used to estimate differences in the fatty acid (FA) profiles in population studies. Infrared spectroscopy in combination with MCR provides a robust method for assessing a FA profile of human adipose tissue. Practical applications: This study has highlighted the use of MCR to enhance the information obtained from infrared spectra. This new approach provides a robust method for assessing a FA profile of human adipose tissue lipids. 相似文献
19.
Dietary sandalwood seed oil modifies fatty acid composition of mouse adipose tissue, brain, and liver 总被引:1,自引:0,他引:1
Sandalwood (Santalum spicatum) seed oil, which occurs to about 50% of the weight of the seed kernels, contains 30–35% of total fatty acids (FA) as ximenynic
acid (XMYA). This study was designed to obtain basic information on changes in tissue FA composition and on the metabolic
fate of XMYA in mice fed a sandalwood seed oil (SWSO)-enriched diet. Female mice were randomly divided into three groups,
each receiving different semisynthetic diets containing 5.2% (w/w) fat (standard laboratory diet), 15% canola oil, or 15%
SWSO for 8 wk. The effects of SWSO as a dietary fat on the FA composition of adipose tissue, brain, and liver lipids were
determined by analyses of FA methyl ester derivatives of extracted total lipid. The FA compositions of the liver and adipose
tissue were markedly altered by the dietary fats, and mice fed on a SWSO-enriched diet were found to contain XMYA but only
in low concentration (0.3 3%) in these tissues; XMYA was not detected in brain. Oleic acid was suggested to be a principal
XMYA biotransformation product. The results were interpreted to suggest that the metabolism of XMYA may involve both biohydrogenation
and oxidation reactions. 相似文献
20.
Conjugated linoleic acid (CLA) is a collection of octadecadienoic fatty acids that have been shown to possess numerous health
benefits. The CLA used in our study was produced by the photoisomerization of soybean oil and consists of about 20% CLA; this
CLA consists of 75% trans–trans (a mixture of t8,t10; t9,t11; t10,t12) isomers. This method could be readily used to increase the CLA content of all soybean
oil used as a food ingredient. The objective of this study was to determine the effects of trans–trans CLA-rich soy oil, fed as a dietary supplement, on body composition, dyslipidemia, hepatic steatosis, and markers of glucose
control and liver function of obese fa/fa Zucker rats. The trans–trans CLA-rich soy oil lowered the serum cholesterol and low density lipoprotein–cholesterol levels by 41 and 50%, respectively,
when compared to obese controls. Trans–trans CLA-rich soy oil supplementation also lowered the liver lipid content significantly (P < 0.05) with a concomitant decrease in the liver weight in the obese rats. In addition, glycated hemoglobin values were improved
in the group receiving CLA-enriched soybean oil in comparison to the obese control. PPAR-γ expression in white adipose tissue
was unchanged. In conclusion, trans–trans CLA-rich soy oil was effective in lowering total liver lipids and serum cholesterol. 相似文献