The Effect of Barley β-Glucan Fiber Fractions on Starch Gelatinization and Pasting Characteristics

ABSTRACT: We evaluated the effects of extraction treatment on the yield and composition of β-glucan-rich fiber fractions from barley, and the subsequent influence of these fractions on the thermal properties and pasting behavior of starch pastes. Extraction treatment affected the yield of barley β-glucan (BBG) fiber fraction, and β-glucan recovery efficiency ( P < 0.05). The level of inclusion of these fractions in starch-water dispersions had a significant influence on the pasting characteristics such as peak viscosity (PV), breakdown (BD), and final viscosity (FV) development (as determined using a Rapid Visco Analyser). Substitution of 5% wheat starch with the fiber fractions decreased PV, BD, and FV in comparison to the control starch ( P < 0.05). Substitution of 5% wheat starch with BBG fiber fraction resulted in a decrease in enthalpy (J/g) values compared with the control ( P < 0.05). A reduction in pasting characteristics may be associated with a reduced enthalpy of starch gelatinization and retention of the integrity of the starch granule. The reduction in PV may also indicate a reduced degree of starch granule swelling.     

Isolation,composition, morphological and pasting properties of starches from rice cultivars grown in Nigeria

The isolation, composition, morphology and pasting properties of rice starches from different rice cultivars (N2R, Nerica II rice; IGR, Igbemo rice; ILR, Ilaje rice; and EAR, Efon Alaye rice) were studied. The starches were isolated from their flours by using a modified deproteination method in 0.1% NaOH. The highest starch yield of 65% was obtained from EAR with a residual protein of 0.41% and lowest starch yield of 45.70% from IGR with a residual protein of 0.42%. The AAM concentration of rice starches ranged from 21.88 to 26.04%. The sizes of the starch granules obtained from SEM were between 3 and 8 µm, some of the granules were individual (single) while others were fused (compound granules). The starch granules were small, polygonal and irregular in shape. The pasting parameters were evaluated using rapid visco analyser (RVA). Significant differences were observed in individual pasting parameters such as peak viscosity (PV), breakdown viscosity (BV), final viscosity (FV) and setback viscosity (SV). Cooked ILR starch had the highest PV (279.69 RVU; rapid viscosity units) and BV (52.50 RVU) when compared to the other starches. In contrast, N2R showed the highest FV (329.92 RVU) and SV (102.50 RVU), the latter due to the high concentration of AAM. The results revealed that cultivar has an effect on composition and pasting properties of rice starch.     

Correlations of the Composition,Minerals, and RVA Pasting Properties of Various Potato Starches

Multiple linear regression equations were developed to correlate the granule size distribution, composition, and minerals content with the Rapid Visco Analyser (RVA) pasting properties of potato starches of different cultivars. From the experimental values and the values of the regression coefficients, it could be observed that a higher level of amylose resulted in a lower peak viscosity (PV) and breakdown (BD) and higher setback (SB) and peak viscosity temperature (PVT) in the potato starches. The reverse was observed when the phosphorus content was higher. Smaller granule size was associated with a decrease in the PVT, whereas larger granules demonstrated higher PV, BD, and SB values in the potato starches. A higher calcium content was associated with a decrease in PV and an increase in BD, SB, and PVT. On the other hand, higher potassium was associated with an increase in PV, BD, SB, and PVT. The sodium contents demonstrated a higher PV and SB, and the reverse was observed in the BD and PVT. It is difficult to conclude the precise role of magnesium ions to the RVA properties although as divalent cations, they may have similar effect as calcium. From the positive and negative values of the regression coefficients it was shown that the correlation is useful for determining more precise values of the RVA pasting properties using a multiple linear regression equation. The correlations were also useful for predicting the effects of the starch composition and minerals on the RVA pasting properties.     

Chemometric Analysis of the Gelatinization and Pasting Properties of Long‐grain Rice Starches in Relation to Fine Structure

Chemometric tests were carried out to better understand the multidimensional facet of starch fine structure‐relationship concerning gelatinization and pasting properties. With Ward's hierarchical cluster analysis 20 long‐grain rice starch samples were sorted out into three clusters based on similarities in functional properties, particularly, paste peak (PV) and final viscosity (FV). The three clusters (arbitrarily named Clusters A, B, and C) exhibited a pasting profile trend of PV<FV, PV˜FV, and PV>FV, respectively. Cluster A samples were also lower in peak temperature, range and enthalpy of gelatinization, and swelling power. These attributes were associated with higher amylose content (AM), β‐amylolysis limit, and percentage of B1 chains (DP13‐24), but lower amylopectin weight‐average molar mass (M_w) and percentage of A chains (DP6‐12). A 5‐variable linear discriminant function correctly predicted 85% of the Ward's cluster membership of the individual cultivars. The discriminant function included the variables A, B1, and B2 (DP25‐36) chains, average chain length (ACL), and gyration radius (R_z). Fine structure variance was fully explained by a total of nine principal components, with the first three components cumulatively accounting for 74%. The leading variables included in the three rotated components pertained to amylopectin chain length distribution (A, B2, and B3+ or DP≥37 chains, and ACL) and amylopectin molar mass (M_w, R_z, and polydispersity). AM and M_w were loaded most frequently in the 4‐variable, best‐fit linear regression models for predicting gelatinization and pasting properties. A combination of at least two fine structure variables controls the functionality of rice starch.     

渗透压对交联玉米淀粉反应影响

为了研究渗透压与取代度和淀粉糊粘度之间关系,用氯化钠和硫酸钠作为玉米淀粉交联反应渗透压促进剂时,取代度(R~2分别为0.9851和0.9894)和起始糊化温度(R~2分别为0.9689和0.8238)都随渗透压增大而线性增加。当用氯化钠作为渗透压促进剂时,糊的峰值粘度和最终粘度都随渗透压增加而线性减小(R~2分别为0.9452和0.9468),破碎度在渗透压为164.39 atm时为0,继续增大渗透压后仍然为0;当硫酸钠作为渗透压促进剂时,峰值粘度和最终粘度随渗透压增大先增加后减小,破碎度随渗透压增加而线性减小(R~2为0.9295)。     

Structural Transformation of Sago Starch by Heat‐Moisture and Osmotic‐Pressure Treatment

Sago starch was modified by osmotic‐pressure treatment (OPT) and heat‐moisture treatment (HMT) and physicochemical characteristics were compared. In OPT, sago starch was suspended in saturated sodium sulfate solution and heated for 1 h at 100, 110 and 120°C, corresponding to a calculated osmotic pressure of 32,728, 33,640 and 34,552 kPa (assuming sodium sulfate dissociates completely), respectively, and in HMT, sago starch with 20% moisture content was used. Change of X‐ray diffraction pattern from C‐type to A‐type was obtained for OPT and HMT starch at 110°C and 120 °C, respectively. RVA viscograms of both OPT and HMT starch exhibited a decrease of peak and breakdown viscosity but increase of final viscosity and pasting temperature. Onset (T_o), peak (T_p), and conclusion temperature (T_c) of gelatinization of both OPT and HMT starch increased significantly with increase of treatment temperature. Biphasic broadening of T_p was observed for HMT starch indicating an inhomogeneous heat transfer during HMT. The observed narrow peaks of DSC curves indicated better homogeneity of OPT. These properties suggest that OPT starch is more suitable for large‐scale production.     

Using Ozonation and Amino Acids to Change Pasting Properties of Rice Starch

ABSTRACT:  In this study, the effects of ozonation and the addition of amino acids on rice starches were determined in terms of pasting properties using a rapid visco-analyzer. Results from viscosity analysis showed that 30-min ozone treatment on commercial rice starch exhibited the greatest swelling extent among the treatments and least retrogradation tendency. The control pure oxygen treated sample had the best cooking stability. The addition of lysine (6%) to 30-min ozonated commercial rice starch significantly reduced peak viscosity (PV), minimum viscosity (MV), and final viscosity (FV) by 918, 1024, and 1023 cP, respectively. Moreover, it decreased Ptime, resulting in the faster swelling upon heating and less rigid gel formation upon cooling. Furthermore, the presence of lysine in 30-min ozonated starch isolate (WSI) also significantly reduced PV, MV, FV, pasting time, and total setback (TSB) and produced starch gel with the best cooking stability and the least retrogradation tendency. Ozonated starch exhibited similar pasting properties to those from oxidized starches treated with low concentrations of chemical oxidizing agents. The combination of lysine with ozonation resulted in pasting properties similar to starches treated with high levels of chemical oxidizing agents. The ozonated starch could be used as a thickening agent, whereas ozonated starch with lysine might be an alternative for a highly chemically oxidized starch. Therefore, ozonation alone or the combination of ozonation and addition of lysine might be used to develop new starch ingredients with various functionalities without using typical chemical modifications.     

Effects of Salts on the Reaction of Normal Corn Starch with Propylene Oxide

The objective of this research was to develop a greater understanding of the mechanism by which salts inhibit gelatinization of starch granules. Using hydroxypropylation of corn starch in the presence of sodium sulfate under conditions employed industrially as the control reaction, reactions with propylene oxide were done with different salts at levels that matched the control reaction in anion molal concentration, cation molal concentration, and total ion molal concentration. Salts used were selected based upon their position in the lyotropic series. Reactions were characterized by determining reaction efficiency and ion concentrations. Sodium and potassium citrates, salts exhibiting a strong lyotropic effect, were extremely effective as gelatinization inhibitors, but resulted in low reaction efficiency. Salts without a strong lyotropic effect (sodium and potassium chlorides) exhibited a weak protective effect, probably at least in part based upon generation of a Donnan potential. In the hydroxypropylation reaction, sodium citrate greatly reduced swelling; sodium chloride was ineffective as a swelling inhibitor, resulting in pasting of granules, and sodium sulfate allowed restricted swelling, most likely as a result of a moderate lyotropic effect and some generation of a Donnan potential. That reaction efficiency parallels granule swelling was confirmed.     

Correlation between the compositional and pasting properties of various potato starches

Multiple linear regression equations were used to develop the correlation between the compositional and rapid visco-analysis (RVA) pasting properties of various potato starches. The amylose of potato starches had a negative correlation with the peak viscosity (PV) and breakdown (BD) and a positive correlation with the setback viscosity (SV) and peak viscosity temperature (PVT). By contrast, phosphorus had a positive correlation with PV, BD, and SV and a negative correlation with PVT. In addition, the median granule size had a positive correlation with PV and BD. By contrast, a negative correlation of the median granule size was observed with SV and PVT. The correlation coefficients of amylose–phosphorus, amylose–granule size, and phosphorus–granule size interactions indicated that amylose had more influence than had phosphorus or had the median granule size on PV and BD. Furthermore, amylose had a greater influence than had the granule size on SV and PVT. Similarly, amylose had more influence than had phosphorus or had the median granule size on PVT. However, the correlation developed in this study was useful for predicting the influence of a specific component and the compositional interaction on the RVA pasting properties.     

Effects of Compositional and Granular Properties on the Pasting Viscosity of Rice Starch Blends

The changes in swelling power and pasting properties of suspensions of starch blends were studied in dependence on starch composition and at various isothermal temperatures (T_iso). Samples were prepared by mixing rice starches from Kaoshiung Sen 7 (KSS7, a high‐amylose‐content variety) and Taichung waxy 70 (TCW70, a waxy variety). Generally, mixing these starches at a comparable ratio caused significant decrements in overall swelling power, onset temperature of gelatinization or viscosity rise, and final viscosity of hot pastes after 30 min of isothermal stirring. Notable increases in the peak and conclusion temperatures of gelatinization and in the peak viscosity of the pastes were also observed. Generally, all viscosity parameters of the blends showed two linear dependencies on the starch composition, the graphs intersecting at a critical starch composition depending on the parameters concerned. The onset temperature of viscosity increase was related to the volume fraction of swollen granules when they were just closely packed in suspension. The volume fraction of KSS granules was smaller than that of TCW70. In addition, the rate of viscosity increase and the peak viscosity of the starch suspensions could be well described in terms of the swelling power (Q) when Q ≤ ∼27, and depending on the T_iso examined. From the photomicrographs of starch suspensions, the extensive shear‐induced disintegration of TCW70 granules in the co‐existence of rigid KSS7 granules was evident and in turn responsible for the reduced final viscosities of the hot pastes obtained from starch blends.     

Gelatinisation Characteristics of Cassava Starch Settled in the Presence of Different Chemicals

Addition of chemicals during the extraction of cassava starch for enhancing the settling rate, whiteness and compactness of the settled starch is an accepted commercial practice. The effect of addition of selected chemicals such as acids (sulphuric and hydrochloric acids), bleaching and oxidising agents (sodium metabisulphite and sodium hypochlorite) and alum during settling on the thermal and pasting properties of the cassava starch was examined. Treatment with sulphuric acid produced a noticeable increase in all DSC gelatinisation parameters, viz. onset gelatinisation temperature (T_o), temperature at peak minimum (T_p) and end temperature (T_e), with increasing concentration of acid, while only a marginal shift could be obtained even at higher concentration of hydrochloric acid. However, no major effect resulted from treatment with sodium metabisulphite, sodium hypochlorite and alum. The gelatinisation enthalpy was hardly affected by the treatments. An exception was hydrochloric acid, which brought about a perceptible decrease in enthalpy at higher concentrations indicating that starch crystallinity is influenced to a small extent by hydrochloric acid. Pasting characteristics studied using a Rapid Visco Analyser showed that sulphuric acid, even at the lowest concentration (5 mM), considerably affected the structural characteristics of cassava starch, while hydrochloric acid induced similar effect only at higher concentrations. Alum reduced the paste viscosity while the bleaching agents (sodium metabisulphite and sodium hypochlorite) were not so effective in modifying the starch viscosity characteristics.     

The effect of rice variety and starch isolation method on the pasting and rheological properties of rice starch pastes

The effect of rice variety and starch isolation method on the pasting and rheological properties of rice starches was evaluated. One waxy and three non-waxy rice varieties from California with a range of amylose contents of 1.6–26.5% and four methods of isolation were evaluated. A rotational rheometer (RR) was used to measure the pasting and rheological properties of starch dispersions (8% w/w). The RR pasting curves had similar shapes to those from a rapid visco-analyzer (RVA). The four treatments used for isolating starch were a protease, NaOH (0.1% and 0.4%), or sodium dodecyl sulfate (SDS) (1.0%). The NaOH (0.4%) and SDS treatments were found to reduce the peak pasting temperatures of the non-waxy starches as compared with the protease and NaOH (0.1%) treatments. The same trend of the treatments was found with the elastic moduli, low shear viscosities, and yield stresses of the non-waxy starch pastes measured at 65 °C, immediately after pasting. The elastic moduli of the waxy starch pastes appeared to be least affected by method of starch isolation, but the low shear viscosity and yield stress of the protease starch paste was significantly higher than the paste from the other three treatments. Overall, the method of rice starch isolation was found to affect the gelatinization and rheological characteristics of hot rice starch paste.     

Influence of molecular structural characteristics on pasting and thermal properties of acid-methanol-treated rice starches

Rice starches from TKW1, TNG67 and TCS17 varieties, differing widely in amylose contents (0.1, 18.3 and 29.2%) were treated at 45 °C for 1 h in methanol containing various amounts of HCl. The recovery, pasting properties, thermal behaviors, molecular size and chain length distribution of starch were observed. Starches exhibited widely different pasting and thermal behavior upon acid-methanol treated (AMT). Degradation of starches upon AMT affected the leaching extent and chain length of amylose. No obvious changes were found on chain length and content of chain fractions of amylopectin. The pasting viscosity of rice starch decreased with increasing concentration of HCl, and the pasting profiles depended on the variety of rice. The pasting profile of AMT-TNG67 starch showed a two-step increasing pattern during heating, while TKW1 and TCS17 starches showed smoothly increasing pasting curves. The relationship between pasting patterns of AMT-TNG67 starches with amylose leaching and two stages of swelling behavior of starch granules was investigated. Results indicated that the pasting of starch granules depend on the amount, as well as the chain length, of amylose in granules.     

In vitro digestibility,pasting, and structural properties of starches from different cereals

Starches isolated from seven different cereals were evaluated for their composition, physicochemical, in vitro digestibility, structural, morphological, and pasting properties. The in vitro starch digestion rate and estimated glycemic index (GI) of cereal starches were evaluated along with the impact of cooking on starch digestion. The cooking of starch slurries increased the rapidly digestible starch content from a range of 34.7–54.4% to a range of 60.5–78.5%. On the basis of hydrolysis index, the GI ranged from 83.6 to 91.8 and after cooking it increased from 95.1 to 98.6 for different cereal starches. Both the swelling power and solubility showed an increasing trend with rising temperature. Paste clarity of starches negatively correlated with fat content. The amylose content of various starches ranged from 17.7 to 24.7% and was negatively correlated to crystalline index (r = -0.975, p ≤ 0.05). XRD pattern revealed A-type pattern of crystalline starch, where crystallinity index ranging between 28.2 to 44.9%. FTIR revealed slight differences among chemical bonding of starches from different cereals. From scanning electron micrograph observations, wheat and barley starch granules proved smoother as compared to other cereal granules. Barley contained the highest (27.5 µm) and rice had the lowest (10.2 µm) size starch granules. The pasting properties were significantly (p ≤ 0.05) different for different cereal starches. Peak, breakdown, and final viscosities were the highest for maize starch (1725, 384, and 2112 mPa.s, respectively), whereas rice and oats exhibited the highest trough and setback viscosities (1420 and 954 mPa.s, respectively).     

氨基酸对大米淀粉糊化和流变性质的影响

以赖氨酸(lysine,Lys)、天冬氨酸(aspartic acid,Asp)和丙氨酸(alanine,Ala)分别代表带正电、负电、不带电3类氨基酸,采用快速黏度分析仪和流变仪研究氨基酸对大米淀粉糊化和流变性质的影响。结果表明,Lys和Asp均显著提高了大米淀粉的峰值黏度和崩解值(P0.05),但是降低了淀粉的最终黏度和回生值,而Ala对淀粉的糊化性质影响微小,在一定浓度范围内无显著差异(P0.05)。因此,带电的2种氨基酸(Lys和Asp)比不带电氨基酸(Ala)对淀粉糊化性质影响更强。此外,3种氨基酸对大米淀粉糊流变性质影响不同:动态流变学实验中,添加Lys和Ala使淀粉凝胶更具弹性,而Asp使淀粉凝胶变弱;静态流变学实验中,运用幂定律τ=kγm,对剪切应力τ和剪切速率γ进行了拟合,结果表明,原淀粉与添加氨基酸的淀粉的m值均小于1,表明淀粉及其氨基酸混合物都属于假塑性流体,并且Lys使淀粉假塑性增强,而Asp则相反。     

Effect of sowing time on protein quality and starch pasting characteristics in wheat (Triticum aestivum L.) genotypes grown under irrigated and rain-fed conditions

The effect of varying sowing time on wheat flour protein quality and starch pasting characteristics was studied in two genotypes differing in their water requirements for two years under irrigated and rain-fed conditions. Delayed sowing associated with high temperature during grain filling resulted in an overall increase in total grain protein. Low temperature prevailing under early sown conditions enhanced insoluble glutenin (IG) but reduced total protein accumulation in both the genotypes. IG and residue proteins increased but soluble glutenin and amylose decreased under rain-fed conditions. Amylose, hold- and final viscosity (FV) increased but setback decreased with change in normal sowing time. Peak viscosity (PV) increased under late sown conditions. Pasting temperature, setback, FV and breakdown (BD) were enhanced under rain-fed conditions. This study inferred that by selecting suitable irrigation practices and different sowing times for subjecting the crop to desirable temperature range, flour properties of given cultivars can be manipulated.     

复合交联剂对微波辅助制备马铃薯交联淀粉性质的影响

以三偏磷酸钠、三聚磷酸钠为复合交联剂,采用微波辅助法制备马铃薯交联淀粉,对交联淀粉的理化性质进行测定,并将其结果与复合交联剂水浴法、单一交联剂微波辅助法制备得到的交联淀粉的性质进行比较,探讨复合交联剂与微波技术对马铃薯淀粉性质的影响。结果表明,采用复合交联剂、微波辅助制备的交联淀粉的结合磷含量与抗性淀粉含量高、膨胀度和溶解度低、热稳定性与抗剪切稳定性好、更容易回生老化。扫描电镜结果显示,采用3种方式制备得到的马铃薯交联淀粉颗粒表面均变得粗糙、出现凹槽,而复合交联剂微波辅助法制备得到的交联淀粉颗粒还出现了断裂、聚集的现象。     

Characterisation of starches separated from sorghum cultivars grown in India

Starches from 15 Indian sorghum cultivars were separated and evaluated for physicochemical, morphological, thermal, retrogradation, pasting and textural properties. The morphological characterisation revealed the presence of irregular-polyhedral as well as spherical shaped granules. A wide variation in amylose content ranging from 11.2% to 28.5% was observed. Thermal, retrogradation, pasting and textural characteristics also showed significant differences amongst all the starch cultivars. Principal component analysis was carried out to extract five principal components that could explain 75% of the total variance. The first two principal components PC1 (T_o, T_p, T_c and ΔH_gel) and PC2 (amylose content, range of gelatinisation, PHI and pasting and textural properties) could explain a cumulative variance of 44%, indicating the importance of amylose, thermal and textural properties on the sorghum starch functionality.     

氯化钠对马铃薯淀粉糊化特性的影响

以马铃薯淀粉为研究对象,研究氯化钠对淀粉糊特性的影响。结果表明:随着氯化钠添加量的增加,马铃薯淀粉起始糊化温度和峰值温度升高,峰值粘度和最终粘度呈现先下降后略微上升的趋势,且淀粉糊的热稳定性提高;氯化钠使淀粉糊的透明度下降,且在添加量为2%时透明度最低;马铃薯原淀粉糊不易凝沉,氯化钠的添加使淀粉糊出现较大的凝沉现象,随着氯化钠添加量的增多凝沉现象加剧,放置时间越长凝沉现象越趋于稳定;氯化钠增大淀粉的溶解度和膨胀度,且不同质量分数的氯化钠对其的影响不同;最后,氯化钠的加入提高淀粉糊的冻融稳定性。     

Gelatinisation Properties of Cassava Starch in the Presence of Salts,Acids and Oxidising Agents

The effect of various cations, anions, acids and oxidising agents on the gelatinisation properties of cassava starch was studied and wide variation was observed in the pasting and swelling properties. Sodium chloride and sodium thiosulphate slightly lowered the peak viscosity of cassava starch with increase in concentration, whereas with sodium sulphite, calcium chloride and sodium hypochlorite, a more significant decrease in the viscosity and breakdown was observed. Ferrous sulphate, alum, aluminium chloride and acids enhanced the peak viscosity at lower concentrations followed by a decrease at 1% and above. Sodium metabisulphite elevated the viscosity at 0.05 and 0.1%, while sodium hypochlorite lowered the viscosity and breakdown. DSC studies showed that aluminium chloride, sodium sulphite, and sodium thiosulphate brought about an increase in T_onset, T_end and ΔH values at higher concentrations. A decrease in swelling volume was observed from 0.05 to 1% concentration of sodium chloride and above that there was a gradual increase. With sodium sulphite, a significant increase in swelling volume was observed at 2.5 and 5% levels. With 0.05 and 0.1% aluminium chloride and sodium metabisulphite an increase in swelling volume was noticed, but at higher concentrations there was a drastic fall. Sodium chloride, sodium sulphite, sodium thiosulphate and calcium chloride brought about decrease in starch paste clarity with increase in salt concentration, whereas ferrous sulphate, aluminium chloride, sodium hypochlorite, hydrochloric acid and acetic acid improved the starch paste clarity.