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1.
Marsaro AL Souza RC Della Lucia TM Fernandes JB Silva MF Vieira PC 《Journal of chemical ecology》2004,30(9):1771-1780
The response of Atta sexdens rubropilosa Forel workers to essential oils, epicuticular wax and hexane, dichloromethane, ethyl acetate, and methanol extracts of Eucalyptus maculata was evaluated. Hexane extracts of E. maculata interfered with the recognition mechanism among workers. The main active compounds identified from this plant were the sesquiterpenes elemol and beta-eudesmol. These compounds may be responsible for the resistance of this species to ant attack. 相似文献
2.
T. R. Govindachari G. Suresh B. Banumathy S. Masilamani Geetha Gopalakrishnan G. N. Krishna Kumari 《Journal of chemical ecology》1999,25(4):923-933
Isolation and characterization of B,D-seco limonoids from Swietenia mahogani have been described. Seven limonoids from S. mahogani and five limonoids from Khaya senegalensis were tested for antifungal activity against the groundnut rust Puccinia arachidis. Mexicanolide, 3-acetoxy-mexicanolide, 3-hydroxymexicanolide, 2,3-dihydroxymexicanolide from K. senegalensis, and 6-acetylswietenine and 6-acetyl-3-tigloylswietenolide from S. mahogani effectively reduced the number of rust pustules on detached groundnut leaves. Possible correlation of structure to antifungal activity is discussed. 相似文献
3.
Antifungal activity of volatile fractions of essential oils from four aromatic wild plants in israel 总被引:2,自引:0,他引:2
Moshe Shimoni Eli Putievsky Uzi Ravid Reuven Reuveni 《Journal of chemical ecology》1993,19(6):1129-1133
Essential oils were extracted fromMajorana syriaca, Satureja thymbra, Micromeria fruticosa, andSalvia triloba, and their volatile fractions were tested for their antifungal activity against the soil-borne pathogensFusarium oxysporum andMacrophomina phaseolina and the foliar pathogensBotrytis cinerea andExserohilum turcicum. Results showed a fungistatic effect of 1, 2.5, and 5l of the various essential oils on fungal mycelium growth. The most significant effect was exerted by essential oils extracted fromM. syriaca, which inhibited the growth ofB. cinerea by 44% and of all the other fungi tested by 100%.Contribution from the Agricultural Research Organization, the Volcani Center, Bet Dagan, Israel. No. 3555-E, 1992 series. 相似文献
4.
Phytotoxin Production and Phytoalexin Elicitation by the Phytopathogenic Fungus Sclerotinia sclerotiorum 总被引:4,自引:0,他引:4
The fungus Sclerotinia sclerotiorum (Lib.) de Bary causes rot disease in a vast range of plant families, including Cruciferae (Brassicaceae). We investigated the production of phytotoxins by S. sclerotiorum by using a bioassay-guided isolation, as well as the phytoalexins produced by the resistant wild crucifer Erucastrum gallicum under elicitation by S. sclerotiorum and other agents. We established for the first time that S. sclerotiorum produces a somewhat selective phytotoxin, sclerin, which is phytotoxic to three cruciferous species (Brassica napus, B. juncea, and Sinapis alba) susceptible to Sclerotinia stem rot disease, causing severe necrosis and chlorosis, but not to a resistant species (Erucastrum gallicum). In addition, we have shown that oleic acid, the major fatty acid isolated from sclerotia of S. sclerotiorum is responsible for the toxic activity of extracts of sclerotia to brine shrimp larvae (Artemia salina). Phytoalexin elicitation in leaves of E. gallicum led to the isolation of three known phytoalexins: indole-3-acetonitrile, arvelexin, and 1-methoxyspirobrassinin. Considering that resistance of E. gallicum to S. sclerotiorum is potentially transferable to B. rapa, a susceptible canola species, and that arvelexin, and 1-methoxyspirobrassinin are not produced by B. rapa, these phytoalexins may become useful markers for resistance against S. sclerotiorum. 相似文献
5.
Composition and Antifungal Activity of the Essential Oil of the Brazilian Chenopodium ambrosioides L. 总被引:2,自引:0,他引:2
The antifungal activity of essential oil (EO) from the Brazilian epazote (Chenopodium ambrosioides L.) was evaluated by the poison food assay at concentrations of 0.3%, 0.1%, and 0.05% with eight postharvest deteriorating fungi (Aspergillus flavus, Aspergillus glaucus, Aspergillus niger, Aspergillus ochraceous, Colletotrichum gloesporioides, Colletotrichum musae, Fusarium oxysporum, and Fusarium semitectum). EO components were tentatively identified by Kováts retention indices (RIs) using gas chromatography and gas chromatography combined with mass spectrometry (GC-MS). Growth of all fungi was completely inhibited at 0.3% concentration, and by 90% to 100% at 0.1% concentration. The following 13 tentatively identified compounds (relative percent) accounted for 90.4% of the total volatile oil: alpha-terpinene (0.9), p-cymene (2.0), benzyl alcohol (0.3), p-cresol (0.3), p-mentha-1,3,8-triene (0.2), p-cimen-8-ol (0.6), alpha-terpineol (0.5), (Z)-ascaridole (61.4), piperitone (0.9), carvacrol (3.9), (E)-ascaridole (18.6), (E)-piperitol acetate (0.5), and (Z)-carvyl acetate (0.3). Autobiographic thin layer chromatography of the EO to separate the principal fungitoxic fraction yielded only one fraction that completely inhibited the growth of all test fungi at a concentration of 0.1%. This fraction was characterized by RIs and GC-MS presenting a composition (%) of p-cymene (25.4), (Z)-ascaridole (44.4), and (E)-ascaridole (30.2). The results suggest ascaridoles were the principal fungitoxic components of the EO. 相似文献
6.
Timothy Paulitz Brian Nowak-Thompson Pascale Gamard Evelyn Tsang Joyce Loper 《Journal of chemical ecology》2000,26(6):1515-1524
Pseudomonas aureofaciens (= P. chlororaphis) strain 63-28 is a biocontrol agent active against many soil-borne fungal plant pathogens and shows antifungal activity in culture assays. 3-(1-Hexenyl)-5-methyl-2-(5H)furanone was isolated from culture filtrates of this bacterium. The purified furanone showed antifungal activity against Pythium ultimum, Fusarium solani, Fusarium oxysporum, and Thielaviopsis basicola. The ED50S for spore germination of these fungi were 45, 54, 56, and 25 g/ml, respectively. The compound also inhibited the germ tube growth of Rhizoctonia solani growing from microsclerotia, with an ED50 of 61 g/ml. The compound is the reduced form of furanones previously described from this bacterium: 3-(1-hexenyl)-5-hydroxy-5-methyl-2-(5H)-furanone and 3-(1-hexenyl)-5-hydroxymethyl-2-(5H)-furanone. This volatile antifungal furanone has structural similarity to other antifungal furanones produced by actinomycetes (Streptomyces spp.), fungi (Trichoderma harzianum), and higher plants (Pulsatilla and Ranuculus spp.). This is the first report of 3-(1-hexenyl)-5-methyl-2-(5H)-furanone produced by a bacterium. 相似文献
7.
T. H. Jones H. M. Garraffo M. S. Blum D. M. Everett H. Hastings A. B. Ware 《Journal of chemical ecology》1998,24(1):125-134
The presumed mandibular gland secretions from the workers and gamergates of two colonies of Streblognathus aethiopicus have been found to contain a homologous series of 3,5-dimethyl-2-alkylpyrazines (3,5-dimethyl-2-butylpyrazine, 1; 3,5-dimethyl-2-pentylpyrazine, 2; and 3,5-dimethyl-2-hexylpyrazine, 3). The structures of these compounds were determined from their mass spectra along with a comparison of their GC-FTIR spectra with those of all the isomers of a lower homolog. This is the first time that this combination of techniques has been applied to the study of insect pyrazines. The relative amounts and proportions of these compounds were different for gamergates and workers, with the latter containing 2–10 times more total pyrazines and having a higher proportion of 2 and 3. The possible significance of this finding is discussed. 相似文献
8.
K. Yamaji Y. Fukushi Y. Hashidoko T. Yoshida S. Tahara 《Journal of chemical ecology》1999,25(7):1643-1653
We screened the microorganisms that are present on the surface of Picea glehnii seeds and produced antifungal compounds against Pythium vexans, a fungus that causes damping-off. Four isolates of Penicillium species that produced patulin, citrinin, palitantin, and arthrographol, respectively, were identified from 149 different microorganisms screened. This study is the first step in an examination of the ecological interaction between host conifers and fungi located on the surface of their seeds. 相似文献
9.
Dong J Zhu Y Song H Li R He H Liu H Huang R Zhou Y Wang L Cao Y Zhang K 《Journal of chemical ecology》2007,33(5):1115-1126
This study investigated metabolites with activities against plant parasite nematodes from the fresh-water fungus Caryospora callicarpa YMF1.01026. We obtained three novel tetradecalactone metabolites, caryospomycins A–C, with such activities. The chemical structures of these were determined through NMR spectroscopic analysis and were found
to belong to the 14-membered macrolides with a fused 1,2-dimethoxy-4-hydroxybenzene ring, a rare structure among the resorcylides.
In the in vitro tests, all three compounds exhibited moderate killing activity against the nematode Bursaphelenchus xylophilus. To our knowledge, this is the first report of secondary metabolites in the aquatic fungal genus Caryospora. 相似文献
10.
Greenberg L Aliabadi A McElfresh JS Topoff H Millar JG 《Journal of chemical ecology》2004,30(6):1297-1303
The sex attractant pheromone produced in mandibular glands of queens of the slave-making ant Polyergus breviceps has been identified as a blend of methyl 6-methylsalicylate and 3-ethyl-4-methylpentanol. In field trials, each compound alone was completely unattractive to males, whereas blends of the two compounds attracted hundreds of males within a couple of hours. 相似文献
11.
Headspace extracts obtained from agar plate cultures of two marine bacteria from the North Sea (Germany), Loktanella strain BIO-204 and Dinoroseobacter shibae strain DFL-27, were analyzed by GC-MS. Several -lactones and one -lactone were identified, besides pyrazines and some sulfur compounds. The absolute configuration of the major lactone (R,Z)-dodec-5-en-4-olide, known as buibuilactone, a pheromone of several scarab beetles, was determined by a new catalytic enantioselective synthesis and GC on a chiral stationary phase. Unsaturated lactones in the extracts included (E)-dodec-5-en-4-olide and the regioisomer (Z)-dodec-6-en-4-olide, previously identified as a component of black-tailed deer urine. The pyrazines 2-butyl-3,6-dimethylpyrazine and 2-isopentyl-3,6-dimethylpyrazine were identified by comparison with synthesized material. The latter compound is a known ant pheromone, as is another identified pyrazine, 2-ethyl-3,6-dimethylpyrazine. The striking similarity between insect pheromones and these bacterial volatiles is discussed, suggesting the possibility of more widespread occurrence of symbiosis between microorganisms and insects than previously thought. 相似文献
12.
The chemical composition of the prothoracic defensive secretion of the water beetles Graphoderus cinereus and Laccophilus minutus was examined by gas chromatographic–mass spectrometric analysis of trimethylsilylated gland extracts. The main components are 3,11-dihydroxy-5-pregnan-20-one in G. cinereus and 3,12-dihydroxy-5-pregnan20-one in L. minutus. Fully saturated pregnanes have not been found in the prothoracic defensive glands of dytiscids before. Based on tests with synthetic compounds, their biological role was examined in a feeding assay with the minnow Phoxinus phoxinus. Agar pellets containing either steroid strongly deterred feeding. 相似文献
13.
Asparagus decline syndrome is caused by fungal infection of asparagus roots and crowns byFusarium oxysporum f.sp.asparagi (FOA) andF. moniliforme (FM). Several soil-borne microorganisms have been found inhibitory toFusarium pathogens in other crops. A novelStreptomyces spp. (ME2-27-19A) was isolated from asparagus field soil and found to be inhibitory to FOA and FM in vitro. Solvent extraction of ME2-27-19A and Chromatographic purification of the extract yielded compound(s) that were inhibitory to FOA and FM at 40g/ml. ME2-27-19A extract produced variable control of FOA and FM in vitro, and was phytotoxic at 1000 (g/ml. In soil, ME2-27-19A extract reduced theFusarium population at 100g/ml, but also reduced the asparagus shoot length. 相似文献
14.
Extracts of Flourensia cernua (L): Volatile Constituents and Antifungal, Antialgal, and Antitermite Bioactivities 总被引:2,自引:0,他引:2
Tellez M Estell R Fredrickson E Powell J Wedge D Schrader K Kobaisy M 《Journal of chemical ecology》2001,27(11):2263-2273
The chemical components of tarbush (Flourensia cernua) leaves were fractionated by extracting successively with hexanes, diethyl ether, and ethanol. Volatile profiles of each fraction were identified by using GC-MS. The hexanes fraction contained mostly monoterpenoids, while the ethanol fraction volatiles were primarily sesquiterpenoids. Crude fractions were tested for activity against fungi, algae, and termites. Application of as little as 1 g of the essential oil from the hexanes fraction was sufficient to provide visible antifungal activity in bioautography assays. The diethyl ether fraction showed selective activity against the cyanobacterium responsible for the 2-methylisoborneol-induced off-flavor sometimes associated with catfish farming operations. All three fractions exhibited a high degree of antitermite activity. 相似文献
15.
Hinoki-asunaro (Thujopsis dolabrata Sieb. et Zucc. var. hondai Makino) is a tree endemic in Japan whose seeds produce several terpenoids. We hypothesized that antifungal compounds in seeds
might select for fungi on the root surfaces of T. dolabrata var. hondai seedlings. We examined seed and soil fungi, their sensitivity to methanol extracts of the seeds, the fungi on root surfaces
of seedlings grown in Kanuma pumice (a model mineral soil) and nursery soil, and the frequency at which each fungus was detected
on the seedling root surface. We calculated correlation coefficients between fungal detection frequency on root surfaces and
fungal sensitivity to seed extracts. We also isolated from the seeds the antifungal compound totarol that selected for fungi
on root surfaces. Species of Alternaria, Cladosporium, Pestalotiopsis, and Phomopsis were the most frequently isolated fungi from seeds. Mortierella and Mucor were the dominant fungi isolated from Kanuma pumice, whereas Umbelopsis and Trichoderma were the main fungi isolated from nursery soil. Alternaria, Cladosporium, Mortierella, Pestalotiopsis, and Phomopsis were the dominant fungi isolated from root surfaces of seedlings grown in Kanuma pumice, and Alternaria, Cladosporium, Pestalotiopsis, Phomopsis, and Trichoderma were the main root-surface fungi isolated from seedlings grown in nursery soil. The fungal detection frequencies on root
surfaces in both soils were significantly and negatively correlated with fungal sensitivity to the seed extract. A similar
correlation was found between the fungal detection frequency on root surfaces and fungal sensitivity to totarol. We conclude
that totarol is one factor that selects for fungi on root surfaces of T. dolabrata var. hondai in the early growth stage. 相似文献
16.
The dish pack method, which measures growth inhibition or promotion effects of volatile compounds on germinating seeds, was
applied to measure the antifungal effects of 52 dried samples of spices and herbs against a soil-borne phytopathogenic fungus,
Fusarium oxysporum. Black zira showed the strongest effect, followed by cumin and cardamom. Headspace sampling and gas chromatography–mass spectrometry
analysis of black zira identified seven volatile compounds, γ-terpinene, limonene, p-cymene, β-pinene, α-pinene, cuminaldehyde, and myrcene. Among these, cuminaldehyde and p-cymene showed the strongest antifungal activities against F. oxysporum, suggesting roles in the antifungal activity of black zira. The same compounds also showed antifungal activities against
another soil-borne phytopathogenic fungus, Verticillium dahliae, and foliar phytopathogenic fungi, Botrytis cinerea and Alternaria mali. The total activity calculated from the concentration of cuminaldehyde contained in black zira and its EC50 against F. oxysporum demonstrated that cuminaldehyde is the main antifungal compound detected in black zira. 相似文献
17.
Plants in some 300 genera produce extrafloral nectar (EFN) to attract ants as a means of indirect defence. Among Mesoamerican
Acacia species, obligate myrmecophytes produce EFN constitutively to nourish symbiotic ant mutualists, while non-myrmecophytes induce
EFN secretion in response to herbivore damage to attract non-symbiotic ants. Since symbiotic Acacia ants entirely depend on the host-derived food rewards while non-symbiotic ants need to be attracted to EFN, this system allows
comparative analyses of the function of EFN components in ant nutrition and attraction. We investigated sugar and amino acid
(AA) composition in EFN of two myrmecophytes (Acacia cornigera and Acacia hindsii) and two related non-myrmecophyte species (Acacia farnesiana and Prosopis juliflora). AA composition allowed a grouping of myrmecophytes vs. non-myrmecophytes. Behavioural assays with obligate Acacia inhabitants (Pseudomyrmex ferrugineus) and non-symbiotic ants showed that AA composition affected ant preferences at high but not at low AA/sugar ratios. Most
interestingly, behavioural responses differed between the two types of ants tested: Symbiotic ants showed a clear preference
for higher AA concentrations and preferred nectar mimics with those four AAs that most significantly characterised the specific
nectar of their Acacia host plant. In contrast, non-symbiotic ants distinguished among nectars containing different sugars and between solutions
with and without AAs but neither among nectars with different AA/sugar ratios nor among mimics containing different numbers
of AAs. Our results confirm that both AAs and sugars contribute to the taste and attractiveness of nectars and demonstrate
that the responses of ants to specific nectar components depend on their life style. AAs are a chemical EFN component that
likely can shape the structure of ant–plant mutualisms. 相似文献
18.
Semiochemicals from Fungal Associates of Bark Beetles May Mediate Host Location Behavior of Parasitoids 总被引:3,自引:0,他引:3
In laboratory olfactometer bioassays, females of two hymenopteran parasitoid species, Roptrocerus xylophagorum (Ratzeburg) (Hymenoptera: Pteromalidae) and Spathius pallidus (Ashmead) (Hymenoptera: Braconidae), were attracted to odors from bark or bolts of loblolly pine, Pinus taeda L., colonized by bluestain fungi (genus Ophiostoma) associated with the parasitoids' bark beetle hosts. Mock-inoculated bolts and bark were less attractive or unattractive in these bioassays. Bark infested with host larvae that lacked their fungal and other normal microbial associates was attractive to R. xylophagorum females, but was less so than bark infested with larvae possessing their normal complement of associated microbes. In contrast, in oviposition bioassays, R. xylophagorum females spent approximately equal time searching, made similar numbers of oviposition attempts, parasitized similar percentages of hosts, and laid similar numbers of eggs in bark fragments infested with either associate-free or associate-bearing host larvae. Furthermore, in field bioassays using bluestain-inoculated or mock-inoculated loblolly pine bolts as sources of attractants, the numbers of parasitoids attracted by the two treatments did not differ significantly and the two treatments were less attractive than bolts naturally infested with bark beetle larvae. Whereas our laboratory olfactometer data suggest that bark beetle fungal associates may enhance attraction of some parasitoids, our bioassays with associate-free hosts indicate that associate-produced are not required for short-range host location and parasitization. In addition, our field trials indicated that long-range attraction of parasitoids to the host-fungi-tree complex is not caused simply by an interaction between bluestain fungi and tree tissues. 相似文献
19.
Francesca R. Dani Sara Cannoni Stefano Turillazzi E. David Morgan 《Journal of chemical ecology》1996,22(1):37-48
The long-chain carboxylic acids identified in the sternal gland secretion ofPolistes dominulus andP. sulcifer females were tested individually on three species of ants,Crematogaster scutellaris, Formica cunicularia, andLasius sp., in order to verify if they have a repellent effect. The unsaturated acids (palmitoleic, linoleic, and oleic) act as repellents of all three ant species, while the saturated acids (lauric, myristic, palmitic, and stearic) have no effect. The mixture reproducing the secretion of the sternal glands ofP. dominulus maintained its repellency for at least four days. 相似文献
20.
Greenberg L Tröger AG Francke W McElfresh JS Topoff H Aliabadi A Millar JG 《Journal of chemical ecology》2007,33(5):935-945
Workers of the slave-making ant, Polyergus breviceps, raid nests of Formica ants and return with Formica pupae that mature into worker ants in the slave-makers’ colony. These Formica workers then tend the Polyergus brood, workers, and reproductives. During raids in the mating season, winged virgin Polyergus queens accompany the workers in the raiding columns. During the raid, the virgin queens release a pheromone that attracts
males that quickly mate with the queens. We report the identification, synthesis, and bioassay of the sex attractant pheromone
of the queens as an approximately 1:6 ratio of (R)-3-ethyl-4-methylpentan-1-ol and methyl 6-methylsalicylate. The ants produce exclusively the (R)-enantiomer of the alcohol, and the (S)-enantiomer has no biological activity, neither inhibiting nor increasing attraction to blends of methyl 6-methylsalicylate
with the (R)-enantiomer. 相似文献