Use of UV-C irradiation to prevent decay and maintain postharvest quality of ripe 'Tommy Atkins' mangoes

Ripe mangoes 'Tommy Atkins' were exposed to UV-C irradiation for 10 and 20 min, prior to storage for 14 days at 5 or 20 °C and a shelf-life period of 7 days at 20 °C. UV-C-treated fruit maintained better visual appearance than unirradiated controls. UV-C irradiation for 10 min was the most effective regime in suppressing decay symptoms and maintaining firmness during storage at 5 or 20 °C. Such fruit (treated with UV-C for 10 min) showed greater levels of putrescine and spermidine after cold storage than controls and those treated with UV-C for 20 min. Higher levels of sugars and lower levels of organic acids were observed in mangoes treated with UV-C for 20 min. However, the most effective UV-C treatment (10 min) for reducing decay maintained sugar and organic acid levels similar to the controls. No UV-damage was observed on treated fruits after storage. These results indicate that UV-C irradiation could be used as an effective and rapid method to preserve the postharvest life of ripe mangoes without adversely affecting certain quality attributes.     

Effect of maturation and cold storage on the organic acid composition of myrtle fruits

BACKGROUND: The effect of maturation and senescence on the chemical composition of two myrtle cultivars was studied in mature, overripe and cold‐stored fruits in order to find the most appropriate harvesting period and best storage technology for industrial purposes. RESULTS: After cold storage at 10 °C for 15 days, berry weight loss ranged from 12.5 to 18.4%, with the highest losses in less mature fruits. Titratable acidity decreased during maturation and cold storage in both cultivars. Reducing and total sugars increased during maturation. Anthocyanin concentration increased during maturation but decreased in overripe berries. The major organic acids in myrtle fruits were quinic, malic and gluconic acids. In fresh and cold‐stored fruits, malic acid rose to 3 g kg^?1 and decreased thereafter. Quinic acid peaked at 90 or 120 days after bloom and decreased thereafter to reach low concentrations in mature fruits. CONCLUSION: Cold storage for 15 days at 10 °C does not affect myrtle fruit quality for liqueur production. Anthocyanin concentration is the best indicator of harvest time for industrial purposes. Gluconic acid concentration is high in mature, overripe and cold‐stored berries. This parameter can be used as a marker of the onset of fruit senescence. Copyright © 2012 Society of Chemical Industry     

Harvest parameters to optimise storage life of European plum 'Oullins Gage'

European plum ( Prunus domestica L.) cv. 'Oullins Gage' was harvested according to firmness on five harvest dates. Fruit was analysed at harvest and during storage for weight loss, firmness, total soluble solids (TSS), titratable acidity (TA) and skin colour before and after a 3-day shelf-life period at 20 °C. Significant differences were found among harvest dates in fruit mass, skin colour, firmness, TSS and TA. Fruit mass, TSS and colour b * increased, whereas firmness and TA decreased significantly during the harvesting period. Firmness parameter as a maximum maturity index and TSS:TA (or b * colour, a non-destructive measurement) as a minimum maturity index could be used to distinguish maturity at harvest. It is suggested that the best post-storage quality of 'Oullins Gage' plums would be obtained when fruit is harvested with TSS:TA >9 or b * colour >30, but with a firmness >20N to assure a long storage life.     

Effect of Ozone on Postharvest Quality of Persimmon

ABSTRACT:  The effect of gaseous ozone exposure on the quality of persimmon picked at 2 different harvest dates was evaluated. Fruit from both harvests were continuously exposed to 0.15 ppm (vol/vol) of ozone for 30 d at 15 °C, 90% relative humidity (RH). Then, fruit were submitted to astringency removal treatment (24 h at 20 °C, 98% CO₂) and stored for 7 d at 20 °C (90% RH) in order to simulate shelf-life period. The most important disorder was flesh softening, which took place when fruit were transferred from 15 °C to shelf-life conditions. In the 2nd harvest, where the fruit were harvested with lower firmness, ozone maintained firmness over commercial limits even after 30 d at 15 °C plus shelf-life. Ozone-treated fruit showed the highest values of weight loss, coinciding with the maximum electrolyte leakage (EL) percentage. Ozone did not affect color index (CI), ethanol, total soluble solids (TSS), or pH. Unremarkable differences in acetaldehyde were observed between fruit submitted to ozone treatment and control fruit. No phytotoxic injuries in tissues were observed in ozone-treated fruit.     

Effect of cold storage and ozone treatment on physicochemical parameters,soluble sugars and organic acids in Actinidia deliciosa

The production of kiwi fruits is a dynamic agricultural activity in Corsica (France). The fruits are either consumed directly or used to produce kiwi wine. They are often stored for 4–6 months in industrial freezer chambers at 0 °C or industrial ozone chambers. The aim of this study was to measure physical, chemical and fungicidal parameters, soluble sugars and non-volatile organic acids during storage in each of these types of chamber. Various standard and instrumental methods (physicochemical techniques, HPLC) in conjunction with statistical analysis were used. During storage, the kiwi mass, firmness and acidity decreased, whereas reducing sugar, °Brix and pH increased. There were statistical differences between the two chambers regarding reducing sugar and acidity. The ozone gas treatment had a fungicidal effect on Botrytis cinerea. The major soluble sugar present in the kiwi fruit was fructose, followed by glucose and sucrose. The concentrations of these sugars increased during storage in both air at 0 °C and ozone-enriched air. Organic acids are one of the important factors influencing fruit flavour. Citric and quinic acids predominated over malic, tartaric and ascorbic acids. During storage in the ozone chamber, concentrations of non-volatile organic acids decreased sharply after 25 weeks. Storage at 0 °C enabled better retention of organic acids.     

Effect of salt concentrations and temperature on the quality and shelf-life of brined anchovies

In this study, the shelf-life and quality of anchovies brined at different concentrations and stored both at refrigerator and ambient temperature conditions were investigated. Sensory, microbiological and chemical analyses of samples were made during storage trials for up to 150 days in order to investigate their quality. The samples stored at ambient temperature showed poor quality at the end of the storage trial. The best quality was observed with the samples that were brined at 22 and 26% salt concentrations, and stored at 4 ± 1 °C.     

金丝小枣在生长成熟过程中理化特性的变化规律

本文研究了6个不同生长成熟期(S1~S6)金丝小枣果实的色泽、叶绿素、可溶性糖及其代谢酶活性、有机酸及氨基酸的动态变化,以明确其代谢规律。研究结果表明:枣果生长成熟过程中,叶绿素含量降低,枣果由绿变红;葡萄糖、果糖均逐步积累,而山梨醇含量逐渐降低,蔗糖则在生长发育初期未被检出,其积累主要发生在枣果成熟后期,且其含量最高,金丝小枣为蔗糖积累型果实,蔗糖磷酸合成酶是蔗糖积累的关键酶;金丝小枣为苹果酸型果实,苹果酸随枣果的成熟而积累,而柠檬酸含量先上升后降低;Pro和Asp在枣果成熟过程中被大量合成,是成熟枣果中最主要的两种氨基酸。全红枣的可溶性总糖、糖酸比以及游离氨基酸总量最高,可作为金丝小枣鲜食的最佳采收时期。     

Changes in Postharvest Quality of Swiss Chard Grown Using 3 Organic Preharvest Treatments

ABSTRACT:  Using storage conditions recommended for conventional chard (4 °C, 90% RH and 7 d), the chard treated with some organic preharvest treatments [effective microorganisms, a fermented mixture of effective microorganisms with organic matter (EM–Bokashi + EM), and an auxiliary soil product] lost considerable water (> 2%) and weight (> 25%). These results indicate that organic methods tested produce a vegetable that can not sustain its quality when commercialized through the conventional supply chain. Nevertheless, respiration, color, pH, and titratable acidity practically remained constant during conservation. Ascorbic acid content was constant in chard treated with the different preharvest treatments and collected at 8 wk after sowing (normal harvest). However, the ascorbic acid content of the control chard decreased 60% after 7 d of storage. This vitamin diminished (35%) in chard collected after 19 wk after sowing (late harvest) during the postharvest conservation. The greatest difference in chard quality was registered between sampling dates since chard collected during the late harvest had higher levels of dry matter, sugars, acids, proteins, and ascorbic acid than chard collected during the normal harvest.     

POSTHARVEST QUALITY AND SENSORY ATTRIBUTES OF ‘BYRONGOLD’ AND ‘RUBYSWEET’ PLUMS1

Fruit from two new cultivars of plums, ‘Byrongold’ and ‘Rubysweet’ were harvested at three maturities. Maturity one and two were ripened for three and six days at 20C and 85% relative humidity to determine fruit quality characteristics during storage. Plums were separated into three maturity categories by delayed light emission. Fruit size, weight, hue angle, flesh firmness, soluble solids and concentration of sugars, organic acids and sensory attributes were determined to evaluate stroage quality of the fruit. Significant differences were found between cultivars for sucrose, glucose, fructose and sorbitol. Sugar concentrations varied within the same cultivar by maturity and storage times. In general, acid levels decreased as plums ripened. Panelists preferred “Byrongold” ripened for three days for tartness and juiciness and “Byrongold” ripened for six days for sweetness. “Rubysweet” ripened for three days was preferred for sweetness, tartness and juiciness. Significant differences were found in peel bitterness between cultivars and days ripened. Unripened maturity 2 ‘Byrongold’ and unripenedd maturity 3 ‘Rubysweet’ had the highest peel bitterness. Hedonic scores showed ‘Rubysweet’.     

STABILITY OF ANTHOCYANINS, PHENOLS AND FREE RADICAL SCAVENGING ACTIVITY THROUGH SUGAR ADDITION DURING FROZEN STORAGE OF BLACKBERRIES

Influence of sugar and mixtures of sugar on color retention of two varieties of blackberries, Thornfree and Cacanska bestrna, were investigated during storage at − 18C for 12 months. After harvest, blackberries were coated with sugars (sucrose, fructose and glucose) and different sugar (sucrose  +  fructose  +  glucose, fructose  +  glucose, fructose  +  sucrose) mixtures. Anthocynin content, total phenol content and free radical scavenging activity of prepared samples were evaluated. Ratio of fruit : sugar was 1:1. The highest anthocyanin retention was in blackberry samples with glucose addition and the lowest in samples with sucrose addition, in both cultivars. Blackberry samples with sucrose addition had even lower retention than samples without sugar addition. Samples with an addition of other sugars had higher retention of anthocyanin than samples stored without sugars.

PRACTICAL APPLICATION

Possibility of phenols retention of blackberries due to sugar addition, during cold storage was investigated. Results showed that glucose had the most positive effect on anthocyanins retention as well as total phenolic compounds content. For that reason, certain amount of sucrose in fruit preserves can be replaced by addition of glucose, and for that reason retention of phenolic compounds can be higher and, as could be expected, more effective against oxidative stress in human body due to intake of product formulation with glucose.     

转录和蛋白质组学分析热空气处理对桃果实采后冷藏期间糖酸和酚类物质代谢的影响

桃果实采后低温贮藏易发生冷害,为研究热空气（hot air,HA）处理对桃果实冷藏过程中调控代谢途径的作用,本实验采用HA处理（40 ℃、4 h）桃果实,于（1±1）℃下贮藏35 d,每隔7 d取样并对可溶性糖、柠檬酸、苹果酸、总酚、总黄酮含量和花色苷含量等指标进行测定,同时选取样品进行转录组学和蛋白质组学分析。结果表明：HA处理可以有效抑制桃果实冷藏期间蔗糖、柠檬酸、苹果酸、总酚和总黄酮含量的下降,并抑制果糖和葡萄糖含量的上升,同时HA显著提高了桃果实花色苷的含量（P＜0.05）。转录组和蛋白质组学分析结果表明,HA处理调控的差异表达基因和蛋白主要集中于碳水化合物代谢和次级代谢物代谢途径;桃果实糖酸代谢变化主要与转化酶、蔗糖合酶、蔗糖磷酸合酶、苹果酸脱氢酶和柠檬酸合酶基因和蛋白的表达有关;HA通过上调苯丙氨酸解氨酶、香豆酸CoA连接酶、查耳酮合酶、二氢黄酮醇还原酶、花青素合成酶和类黄酮葡萄糖基转移酶的表达量,促进酚类、黄酮类和花青素的合成。综上所述,HA处理可以有效延缓桃果实采后糖酸和酚类物质含量的下降,并提高果实花色苷的含量。     

Plum Storability Improved after Calcium and Heat Postharvest Treatments: Role of Polyamines

ABSTRACT: Plums were treated with calcium or heat (45°C) and then stored at 2°C for 28 d. Fruit firmness, ethylene, and CO₂ production rates were investigated. The concentrations of endogenous polyamines (free, conjugated-soluble, and cell wall-bound) were also studied. Both treatments improved fruit firmness by increases in Magness-Taylor force and force-deformation ratio, and decreases in flesh deformation. No differences were found in either ethylene or respiration rate production among fruits due to the low storage temperature. Calcium-treating plums increased the conjugated forms of putrescine (conjugated-soluble and cell wall-bound), which are related to higher firmness. Heat-treated plums mainly increased cell wall-bound spermidine, inducing a greater cell wall stability and plum firmness.     

Quality changes during ripening of plums (Prunus domestica L.)

Quality changes during fruit ripening after the appearance of fruit colour of four Prunus domestica L. plum cultivars, ‘Jojo’, ‘Valor’, ‘Čačanska rodna’ and ‘Čačanska najbolja’, were investigated during 25 or 33 day periods. Fruit samples were analyzed for fruit weight, firmness, soluble solids content, fruit colour, content of sugars (glucose, fructose, sorbitol and sucrose), organic acids (malic, fumaric and shikimic acids), phenolics (neochlorogenic acid, p-coumaroylquinic acid, chlorogenic acid and rutin) and anthocyanins (cyanidin-3-rutinoside and peonidin-3-rutinoside). Ripening resulted in statistically increased fruit weight and soluble solids, decreased fruit firmness, darker colour of fruits, increased concentration of total sugars, decreased concentration of total acids, and increased concentration of anthocyanins. There was no influence of ripening on the content of phenols. The results show significant influences of cultivar on fruit weight, soluble solids content, firmness, fruit colour, concentration of total acids, SUAC index, concentration of total phenols and anthocyanins in European plums.     

Developmental changes of carbohydrates,organic acids,amino acids,and phenolic compounds in ‘Honeycrisp’ apple flesh

The developmental changes of carbohydrates, organic acids, amino acids and phenolic compounds in ‘Honeycrisp’ apple flesh were investigated using GC–MS and HPLC. A total of 12 carbohydrates, 8 organic acids, 20 amino acids, and 18 phenolic compounds were identified and quantified. Each metabolite showed characteristic changes during fruit development, but in general, concentrations of most sugars and sugar alcohols either increased or remained unchanged whereas concentrations of most organic acids, amino acids and phenolic compounds decreased with fruit development, indicating that most sugars and sugar alcohols are synthesised and/or accumulate at a faster or similar rate relative to fruit growth whereas organic acids, amino acids and phenolics are synthesised and/or accumulate at a slower rate relative to fruit growth. On a whole fruit basis, the content of most metabolites increased with fruit development. In the flesh of mature ‘Honeycrisp’ apple, fructose and sucrose and sorbitol are the major sugars and sugar alcohol; malic acid is the major organic acid; aspartic acid, asparagine, glutamic acid, proline, threonine and γ-aminobutyric acid are the major amino acids; and procyanidin B1, procyanidin B2, chlorogenic acid, catechin and epicatechin are the major phenolic compounds, respectively.     

Effect of thermal processing on the texture of canned apricots

The effect of thermal processing on the texture of canned apricots was studied by using the main cultivar canned in Greece, Bebecou. The test temperature ranged from 82 to 95 °C. The loss of hardness was tested immediately after processing and after 3 months storage. The z -value was 16.7 °C and the energy of activation 116.5 kJ mol⁻¹. Some restoration in the hardness was found after 3 months storage, which might be attributable to the absorption of sucrose by the fruit.     

Effect of Harvest Treatment on Biochemical Properties of Farmed Chinook Salmon (Oncorhynchus tshawytscha) Tissue during Frozen and Thawed Storage

ABSTRACT:  Two different harvest procedures were employed to investigate whether the method of harvest has an effect upon deteriorative processes that occur during the frozen storage of Chinook salmon ( Oncorhynchus tshawytscha ) white muscle tissue. These 2 harvest methods, termed "rested"—involving sedation with the aquatic anesthetic AQUI-S™ and "exercised"—a simulated conventional harvest not involving sedation, contrasted levels of activity of the animal prior to and upon slaughter. Rested and exercised harvesting protocols produced tissue in significantly different postmortem physiological states prior to freezing. Rested, postharvest tissue maintained high metabolic energy stores of ATP and glycogen within the tissue, with low concentrations of tissue and plasma lactate. Exercised tissue exhibited near depleted concentrations of ATP and glycogen and a marked lactate accumulation. In both treatments, no significant change in metabolite levels was seen over a 6-mo storage period at −19 °C when tissue was frozen immediately postharvest. Transfer of tissue from frozen temperatures (−80 and −19 °C), to refrigerated (−1 and +4 °C, respectively) resulted in rapid glycolysis, depleting tissue ATP and glycogen stores and increasing tissue lactate concentrations. Metabolic activity was more significant in rested tissue owing to the larger concentrations of metabolic energy stores and occurred at temperatures between −3 and −1.5 °C. During frozen storage (−19 °C), there was an increase in the secondary lipid oxidation product TBARS, but harvest treatment had no effect. However, following transfer from frozen to refrigerated (+4 and −1 °C) storage, rested tissue showed a significant ability to retard the development of TBARS products.     

Efficacy of Pressure-Assisted Thermal Processing, in Combination with Organic Acids, against Bacillus amyloliquefaciens Spores Suspended in Deionized Water and Carrot Puree

ABSTRACT:  Effect of organic acids (acetic, citric, and lactic; 100 mM, pH 5) on spore inactivation by pressure-assisted thermal processing (PATP; 700 MPa and 105 °C), high pressure processing (HPP; 700 MPa, 35 °C), and thermal processing (TP; 105 °C, 0.1 MPa) was investigated.  Bacillus amyloliquefaciens  spores were inoculated into sterile organic acid solutions to obtain a final concentration of approximately 1.3 × 10⁸ CFU/mL.  B. amyloliquefaciens  spores were inactivated to undetectable levels with or without organic acids after 3 min PATP holding time. At a shorter PATP treatment time (approximately 2 min), the inactivation was greater when spores were suspended in citric and acetic acids than in lactic acid or deionized water. Presence of organic acids during PATP resulted in 33% to 80% germination in the population of spores that survived the treatment. In contrast to PATP, neither HPP nor TP, for up to 5 min holding time with or without addition of organic acids, was sporicidal. In a separate set of experiments, carrot puree was tested, as a low-acid food matrix, to study spore recovery during extended storage following PATP. Results showed that organic acids were effective in inhibiting spore recovery in treated carrot puree during extended storage (up to 28 d) at 32 °C. In conclusion, addition of some organic acids provided significant lethality enhancement ( P  < 0.05) during PATP treatments and suppressed spore recovery in the treated carrot puree.     

Formulation and shelf-life of a bottled pawpaw juice beverage

A simple procedure was developed for production of bottled pawpaw beverage juice by peeling and macerating peeled tissue in 25% water, straining through a 0.8-mm sieve, adjustment of juice pH with citric acid and flavour adjustment with sucrose. Fresh juice was optimized for acceptability at pH 3.9 and 10% (w/w) sucrose. Heating for 6 min at 72.2°C was required to achieve commercial pasteurization. Samples of juice were prepared with no preservative, and containing sodium benzoate (125mg/100ml), sodium metabisulphite (50mg/100ml) and sodium metabisulphite/sodium benzoate combination (25mg and 60mg/100ml) for trials in which acceptability, pH, specific gravity, brix, total acidity, vitamin C and biomass concentrations were measured over 90 weeks storage at 10°C and 30.2°C. Sodium benzoate alone extended the shelf-life at 30°C up to 80 weeks but the other preservatives were not effective after 20 weeks. The control juice was already deteriorating by 10 weeks at 30°C. At 10°C all preserved samples were stable up to 80 weeks, although the control deteriorated rapidly after 20 weeks.