PDH-600树脂对多穗柯总黄酮的吸附性能研究

为了研究大孔吸附树脂PDH-600对多穗柯总黄酮的吸附性能及原液浓度、pH值、流速、洗脱剂的种类对树脂吸附性能的影响，采用分光光度法测定多穗柯中总黄酮的含量。测定得PDH-600树脂对多穗柯总黄酮的适宜吸附条件为原液浓度为1.650mg／ml，PH值为5、流速为2BV／h洗脱剂用45％乙醇时，解吸效果较好，PDH-600树脂可用作多穗柯总黄酮的精制方法。     

ADS系列大孔吸附树脂纯化夏枯草穗总黄酮工艺研究

目的研究ADS系列大孔吸附树脂分离纯化夏枯草穗总黄酮的工艺条件及参数。方法以树脂对夏枯草穗总黄酮的吸附量和洗脱率为指标,筛选ADS系列大孔吸附树脂分离纯化夏枯草穗总黄酮的工艺条件。结果 ADS-8型大孔吸附树脂对夏枯草穗总黄酮有较好的吸附分离性能,该树脂分离纯化夏枯草穗总黄酮的最佳工艺条件为：上柱液pH值5.0,1.20 mg/mL夏枯草提取液以流速2.0 BV/h上柱,洗脱剂乙醇体积分数40%,以1.5 BV/h的流速洗脱,收集洗脱液。经上述工艺纯化后,产品总黄酮含量达82.7%,收率66.2%。结论 ADS-8型大孔吸附树脂适于分离纯化夏枯草穗总黄酮。     

山楂降血脂有效部位的纯化工艺研究

研究大孔吸附树脂纯化山楂降血脂有效部位的条件和参数。比较四种大孔吸附树脂对山楂总黄酮、山楂总三萜酸的吸附性能;以树脂吸附量为指标,对大孔吸附树脂纯化山楂降血脂有效部位的工艺进行筛选。AB-8大孔吸附树脂对山楂降血脂有效部位的吸附性能最好,其纯化的最佳条件为：原液浓度0．13g生药/mL,流速为0．5mL／min,pH值为原液pH值（3．5左右）。洗脱剂选择80％的乙醇溶液。洗脱流速为2mL/min。AB-8大孔吸附树脂可用于山楂降血脂有效部位的纯化,纯化后有效部位的纯度达到85％以上。此工艺可行,树脂再生容易。     

大孔树脂富集酸枣仁总黄酮工艺及其体外抗氧化性研究

研究大孔吸附树脂富集纯化酸枣仁总黄酮的最佳条件,并进行了总黄酮体外抗氧化能力的测定。利用静态吸附和动态吸附实验对5种不同极性的大孔吸附树脂进行筛选,并对上样液质量浓度、上样量、洗脱剂体积分数、洗脱剂体积以及洗脱剂流速等条件分别进行考察。采用DPPH自由基和ABTS+自由基的清除率以及铁氰化钾的还原能力作为指标考察纯化后总黄酮的体外抗氧化能力。结果表明AB-8大孔吸附树脂为纯化酸枣仁总黄酮的最佳树脂,纯化工艺为上样液质量浓度1.99 mg/m L,上样量50 m L,洗脱剂体积分数50%乙醇,洗脱剂体积50 m L,洗脱剂流速1 m L/min。抗氧化结果显示总黄酮对DPPH和ABTS+自由基具有明显的清除能力（IC50值为0.70 mg/m L和0.15 mg/m L）,并对铁氰化钾表现出了较强的还原能力。      

大孔吸附树脂分离纯化桂花总黄酮工艺条件研究

目的:研究大孔吸附树脂分离纯化桂花总黄酮工艺条件,为桂花总黄酮的工业化生产提供实验依据。方法:以贵州产桂花为原料,以桂花总黄酮吸附量及回收率等为考察指标,选用AB-8型大孔吸附树脂对桂花总黄酮进行分离纯化,分别采用静态试验、动态试验等考察AB-8型大孔树脂对桂花总黄酮的分离纯化最佳工艺条件及效果。结果:pH值、洗脱剂、温度、上柱液浓度、径高比、流速、总黄酮与树脂质量比等工艺条件对桂花总黄酮的吸附洗脱量、回收率等影响甚大。结论:AB-8型大孔树脂分离纯化桂花总黄酮最佳工艺条件为:上柱液pH4～ 5;洗脱剂为70%乙醇,洗脱剂用量为4倍树脂体积,流速3～ 4 mL/min;上柱总黄酮质量与树脂质量比为1:9.4,上柱液总黄酮浓度为17.86 mg/mL,流速2～ 3 mL/min;冲洗杂质用水体积2～ 3 BV,流速2～ 3mL/min;径高比1.5/21.6;温度升高,吸附量下降但洗脱率加大。     

费菜总黄酮分离纯化工艺的研究

本文采用了AB-8大孔吸附树脂对费菜总黄酮进行分离纯化,利用静态和动态吸附洗脱结合的方法,以AB-8大孔树脂的吸附率和解析率为指标考察了pH、洗脱剂浓度、洗脱时间、吸附流速、洗脱流速、洗脱剂用量、洗脱蒸馏水用量等对费菜总黄酮的分离纯化效果及影响因素。结果表明费菜总黄酮分离纯化的适宜工艺条件:吸附液pH为5.0、洗脱剂为80%乙醇、洗脱时间24h、吸附流速1~2mL/min、洗脱流速2mL/min、洗脱剂用量80mL、洗脱蒸馏水用量100mL。在该条件下,AB-8大孔树脂的饱和吸附量为11mg/g、静态吸附率68.3%、解析率94.5%、费菜总黄酮纯度47.98%。     

大孔吸附树脂分离纯化三叶委陵菜总黄酮的研究

为了除去三叶委陵菜中总黄酮的杂质,选择以大孔吸附树脂对三叶委陵菜黄酮的吸附率、解析率为指标,考察了六种大孔吸附树脂对三叶委陵菜中总黄酮的分离纯化性能,筛选出了性能最佳的大孔吸附树脂,同时采用高效液相色谱法进行分析检测进而表征了最佳型号的大孔吸附树脂对三叶委陵菜中总黄酮分离纯化的效果;分析了原液浓度、pH和树脂用量以及洗脱剂的浓度和pH对吸附解析效果的影响.实验结果表明,大孔吸附树脂D101对三叶委陵菜总黄酮有很好的吸附和解析性能,并确定了最佳的吸附脱附条件为:吸附液浓度为2.0 mg/mL、pH =6、树脂用量与吸附液量比(g/mL)为1∶30;平衡浓度达到0.36 mg/mL时,即树脂已经达到吸附饱和,饱和吸附量为49.1 mg/g干树脂;洗脱剂乙醇浓度为50％,pH =6.     

大孔吸附树脂法纯化槐米总黄酮的研究

对大孔吸附树脂纯化槐米总黄酮的工艺进行了研究.方法是采用优选树脂纯化槐米总黄酮,以分光光度法测定槐米总黄酮含量.结果表明AB-8树脂吸附与纯化槐米总黄酮的效果较好,其工艺参数为:原液上样质量浓度为2.802mg/mL,最大吸附量为30.8mg/g,吸附速度为1 BV/h,上样2次,吸附时间为1 h,洗脱剂为45%乙醇,用量为3BV,洗脱速度为4BV/h,且树脂可重复使用3次.     

大孔吸附树脂纯化艾草总黄酮及其抗氧化活性研究

确定艾草总黄酮初步分离纯化的最佳工艺条件及抗氧化活性。比较6种大孔吸附树脂的静态吸附和解吸附效果,确定最佳吸附树脂并考察其上样液浓度、上样液pH值、吸附温度、上样速度、洗脱流速、洗脱用量对艾草总黄酮吸附及解析附性能的影响。结果表明：AB-8大孔吸附树脂的综合效果最佳,其最佳工艺条件为：上样液浓度为2.5 mg/mL,上样液pH值为4,吸附温度为20℃,上样速度为1.5 mL/min,选用80%乙醇进行洗脱,洗脱流速为1.5 mL/min,洗脱剂用量为100 mL。在此吸附和解吸条件下,艾草总黄酮的纯度由36.1%上升至75.43%,纯度提高了近2倍,纯化效果良好。抗氧化试验结果表明：艾草总黄酮具有一定的抗氧化活性,是一种潜在的天然抗氧化剂。     

应用大孔吸附树脂吸附分离岗稔总黄酮

采用多种型号大孔吸附树脂对岗稔总黄酮进行吸附分离,筛选最佳树脂,考察其静态吸附曲线、动态吸附曲线和动态洗脱曲线,并考察pH、原液浓度对静态吸附的影响以及洗脱剂浓度对静态洗脱的影响。实验结果表明,HPD-700大孔吸附树脂对岗稔总黄酮具有良好的吸附分离作用。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Chlorohydrins of bisphenol A diglycidyl ether (BADGE) and of bisphenol F diglycidyl ether (BFDGE) in canned foods and ready-to-drink coffees from the Japanese market

BADGE.2HCl and BFDGE.2HCl were determined in 28 samples of ready-to-drink canned coffee and 18 samples of canned vegetables (10 corn, 5 tomatoes and 3 others), all from the Japanese market. HPLC was used as the principal analytical method and GCMS for confirmation of relevant LC fractions. BADGE.2HCl was found to be present in one canned coffee and five samples of corn, BFDGE.2HCl in four samples of canned tomatoes and in one canned corn. No sample was found which exceeded the 1mg/kg limit of the EU for the BADGE chlorohydrins. However the highest concentration was found for the sum of BFDGE.2HCl and BFDGE.HCl.H₂O at a level of 1.5mg/kg. A Beilstein test confirmed that all cans containing foods contaminated with BADGE.2HCl or BFDGE.2HCl had at lest one part coated with a PVC organosol.     

European priorities for research to support legislation in the area of food contact materials and articles

A strong science base is required to underpin the planning and decision-making process involved in determining future European community legislation on materials and articles in contact with food. Significant progress has been made in the past 5 years in European funded work in this area, with many developments contributing to a much better understanding of the migration process, and better and simpler approaches to food control. In this paper this progress is reviewed against previously identified work-areas (identified in 1994) and conclusions are reached about future requirements for R&D to support legislation on food contact materials and articles over the next 5 or so years.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the