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1.
BACKGROUND: Orange‐fleshed cantaloupe melons have intense aroma and flavor but are very perishable during storage life. Fresh‐cut processing enhances ethylene‐mediated quality losses. Post‐cutting 1‐methylcyclopene (1‐MCP) application to fresh‐cut cantaloupe was evaluated for its effects on quality attributes, phytochemical content and aroma volatiles. RESULTS: Fresh‐cut cantaloupe (Cucumis melo var. cantalupensis ‘Fiesta’) cubes treated with 1.0 µL L?1 of 1‐MCP for 24 h at 5 °C, packaged in vented plastic clamshells and stored under normal atmosphere at 5 °C for 9 days, preserved their soluble solids, total phenolics, total carotenoids and β‐carotene contents, but significant softening occurred. A significant increase of non‐acetate esters and a decrease of aldehydes occurred during storage. Most quality attributes of fresh‐cut cantaloupe were unaffected by the treatment with 1‐MCP. 1‐MCP‐treated fresh‐cut cantaloupe accumulated higher levels of propyl acetate, 2‐methylbutyl acetate, methyl butanoate, methyl 2‐methyl butanoate, methyl hexanoate, 2‐methylbutyl alcohol and phenethyl alcohol, and lower levels of benzyl alcohol and heptanal than untreated controls. CONCLUSION: Post‐cutting treatment with 1‐MCP affected nine of the flavor‐important volatiles, particularly those derived from the amino acids isoleucine and phenylalanine, but had no practical effect on phytochemicals or other quality attributes. © 2012 Society of Chemical Industry  相似文献   

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The combined effect of γ‐irradiation and refrigeration on the shelf‐life of vacuum‐packaged sea bream (Sparus aurata) fillets was studied by monitoring the microbiological, chemical and sensory changes of non‐irradiated and irradiated fish samples using low‐dose irradiation doses of 1 and 3 kGy. Fish species such as sea bream and sea bass are very popular in the Mediterranean countries due to their high quality characteristics, and their preservation is a constant challenge given their extreme perishability. Irradiation (3 kGy) dramatically reduced populations of bacteria, namely, total viable counts (3 vs 7 log cfu g?1) for the non‐irradiated samples, Pseudomonas spp (<2 vs 7.6 log cfu g?1), H2S‐producing bacteria typical of Shewanella putrefaciens (<2 vs 5.9 log cfu g?1), Enterobacteriaceae (<2 vs 6.0 log cfu g?1) and lactic acid bacteria (<2 vs 3.5 log cfu g?1) after 10 days of storage. The effect was more pronounced at the higher dose (3 kGy). Lactic acid bacteria, Enterobacteriaceae and H2S‐producing bacteria typical of Shewanella putrefaciens showed higher sensitivity to γ‐radiation than did the rest of the microbial species. Of the chemical indicators of spoilage, Trimethylamine (TMA) values of non‐irradiated sea bream increased very slowly, whereas for irradiated samples significantly lower values were obtained reaching a final value of 7.9 and 6.3 mg N per 100 g muscle at 1 and 3 kGy respectively (day 42). Total volatile basic nitrogen (TVB‐N) values increased slowly attaining a value of 67.3 mg N per 100 g for non‐irradiated sea bream during refrigerated storage, whereas for irradiated fish, lower values of 52.8 and 43.1 mg N per 100 g muscle were recorded (day 42). Thiobarbituric acid (TBA) values for irradiated sea bream samples were higher than respective non‐irradiated fish and increased slowly until day 21 of storage, reaching final values of 1.1 (non‐irradiated), 2.0 (1 kGy) and 2.2 mg malonaldehyde kg?1 muscle (3 kGy), respectively (day 42). Sensory evaluation showed a good correlation with bacterial populations. On the basis of overall acceptability scores (sensory evaluation) a shelf‐life of 28 days (3 kGy) was obtained for vacuum‐packaged sea bream, compared with a shelf‐life of 9–10 days for the non‐irradiated sample. Copyright © 2004 Society of Chemical Industry  相似文献   

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This research focused on determining the dose levels suitable for electron beam irradiation of mangoes without detriment to the fruit's quality characteristics. Physicochemical, textural, respiration rates, microstructural, and sensory characteristics of “Tommy Atkins” mangoes irradiated at 1.0, 1.5, and 3.1 kGy using a 10 MeV (10 kW) linear accelerator with double‐beam fixture were determined. Fruits were stored at 12 °C and 62.7% RH for 21 d and evaluated at days 0, 5, 10, and 21. Nonirradiated mangoes served as controls. Irradiation did affect the textural characteristics of mangoes at doses higher than 1.0 kGy. Mangoes exposed to 1.5 and 3.1 kGy were softer and less stiff throughout storage. The radiation‐induced softening of the fruits may be associated with changes in the structural cell such as cracks and depressions on the surface and the breakdown of the cells and its components. Irradiation at 3.1 kGy affected the color of mangoes by the end of storage. Doses up to 1.5 kGy kept respiration rates at a normal level. Irradiation did not affect the specific gravity of mangoes, a parameter associated with fruit maturity levels. No effect of irradiation on pH, water activity, moisture content, acidity, and juiciness of mangoes was detected at the dose levels used in this study. Only fruits irradiated at 3.1 kGy were unacceptable to the sensory panelists in terms of overall quality, texture, and aroma. Electron beam irradiation of “Tommy Atkins” mangoes at 1.0 kGy is the recommended treatment to maintain the overall fruit quality attributes.  相似文献   

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BACKGROUND: The present study investigated the effect of irradiation, active and modified atmosphere packaging, and storage conditions on quality retention of raw, whole, unpeeled almonds. Almond kernels were packaged in barrier and high‐barrier pouches, under N2 or with an O2 absorber and stored either under fluorescent lighting or in the dark at 20 °C for 12 months. Quality parameters monitored were peroxide value, hexanal content, colour, fatty acid composition and volatile compounds. Of the sensory attributes colour, texture, odour and taste were evaluated. RESULTS: Peroxide value and hexanal increased with dose of irradiation and storage time. Irradiation resulted in a decrease of polyunsaturated and monounsaturated fatty acids during storage with a parallel increase of saturated fatty acids. Volatile compounds were not affected by irradiation but increased with storage time indicating enhanced lipid oxidation. Colour parameters of samples remained unaffected immediately after irradiation. For samples packaged under a N2, atmosphere L* and b* values decreased during storage with a parallel increase of value a* resulting to gradual product darkening especially in irradiated samples. CONCLUSION: Non‐irradiated almonds retained acceptable quality for ca. 12 months stored at 20 °C with the O2 absorber irrespective of lighting conditions and packaging material oxygen barrier. The respective shelf life for samples irradiated at 1.0 kGy was 12 months packaged in PET‐SiOx//LDPE irrespective of lighting conditions and 12 months for samples irradiated at 3 kGy packaged in PET‐SiOx//LDPE stored in the dark. Copyright © 2010 Society of Chemical Industry  相似文献   

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We determined the optimum irradiation treatment for decontamination of physiologically mature fresh “Tommy Atkins” mangoes, without detriment to the fruits' sensory and chemical properties. Mangoes were irradiated at 1.0, 1.5 and 3.1 kGy using a 10‐MeV linear accelerator (14‐kW LINAC, double beam mode). Mangoes were stored for 21 days at 12C and 62.7% relative humidity with nonirradiated fruits as controls. Dose distribution within the fruit was determined using Monte Carlo techniques. Irradiation did not affect the overall sensory quality of mangoes at doses up to 1.5 kGy. Only fruits irradiated at 3.1 kGy were unacceptable by the panelists. Irradiation at 3.1 kGy enhanced the fruit's aroma characteristics. Irradiation at all levels caused a significant (P ≤ 0.05) decrease (50–70%) in ascorbic acid content by the end of storage. Mangoes irradiated at 1.5 and 3.1 kGy had slightly higher levels of phenolics than the control (27.4 and 18.3%, respectively). E‐beam irradiation of Tommy Atkins mangoes up to 3.0 kGy causes no detriment to the fruit's overall sensory and chemical quality.  相似文献   

7.
Spices and packaging materials were exposed to γ‐irradiation at a dose of 10 kGy. Luncheon meat was prepared with irradiated or non‐irradiated spices and packaged in irradiated or non‐irradiated packaging materials. Packaged luncheon meat was treated with 2 kGy. Irradiated and non‐irradiated packaged luncheon meat were kept in a refrigerator (1–4 °C) for 12 months. Microbiological, nutritive and chemical characteristics of luncheon were evaluated after processing and during storage; whereas, sensory quality was evaluated only after irradiation. γ‐Irradiation decreased the microbiological counts of spices, packaging materials and packed products and increased its shelf‐life. No significant differences in moisture, protein, fat, pH value, total acidity, lipid peroxide and volatile basic nitrogen were observed as a result of irradiation. Sensory evaluation showed that all the combinations of treated luncheon meats were acceptable. However, the taste, odour, appearance and texture scores of irradiated packaged products were significantly lower than those of non‐irradiated samples.  相似文献   

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Our objectives were to assess the effects of low‐dose irradiation and electrical stimulation on quality parameters of beef from Bos indicus × Bos taurus bulls. Loins from six animals were assigned to either irradiation (2.5 kGy) or electric stimulation (150 V, 50 Hz for 2 min), resulting in four treatments. From each treatment, 2.54‐cm‐thick steaks were cut, packaged in LDPE pouches, and stored at 4 °C for 28 days. Irradiated samples had shelf‐life of 28 days compared with non‐irradiated samples (P < 0.05). However, TBARS values were greater in irradiated samples compared with non‐irradiated samples. Electrically stimulated samples were tender, but had lower water holding capacity and greater redness and drip loss compared with non‐electric stimulated (P < 0.05). Thus, a combination of irradiation and electric stimulation can improve tenderness and reduce aerobic plate count of meat obtained from old and undernourished bulls of Bos indicus×Bos taurus origin.  相似文献   

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ABSTRACT

The microbial keeping quality of fresh‐cut iceberg lettuce was determined after being washed in either cold water (5C) for 3 min or warm water (47C) for 2 min followed by a cold water rinse (5C) and packaged in a modified atmosphere film bag. The lettuce samples were treated with gamma radiation to 0, 1 or 2 kGy while maintaining a refrigerated temperature (4C). The samples were analyzed for total aerobic, total coliform and Enterobacteriaceae counts after refrigerated storage up to 12 days. No difference in aerobic counts was observed between the hot‐ and cold‐washed samples immediately after washing. The coliform and Enterobacteriaceae counts were reduced by 2 log after the warm water wash and no difference for the cold water‐washed sample. The irradiation treatment at 1 kGy reduced the aerobic, coliform and Enterobacteriaceae counts by 2 log for the warm‐washed samples. At the 2‐kGy treatment level, the aerobic and coliform counts were reduced by 3 log for the cold‐washed lettuce, whereas the Enterobacteriaceae counts were reduced by only 2 log. The observed log reductions in bacterial counts after irradiation were maintained for 12 days when stored at 4C. The combination of a cold water wash and irradiation to 2 kGy had the best microbial keeping quality.

PRACTICAL APPLICATIONS

Fresh‐cut lettuce, when washed in either cold or warm water, shows neither an appreciable removal of the microbial load nor a significant increase in the keeping quality when compared with unwashed fresh‐cut controls. Placing the washed lettuce into modified atmosphere packaging (MAP) did not lessen the overall bacteriologic load, and after 12 days of storage at 4C, the microbial counts increased. However, gamma irradiation of the washed, MAP‐stored lettuce to a dose of 2 kGy significantly reduces the overall microbe count, thereby increasing both the shelf life and the safety of the produce. A 2‐kGy dose of gamma irradiation provides a pathogen‐free, long shelf life, fresh‐cut lettuce that is bacteriologically safer and sensorially indistinguishable from bagged, nonirradiated, fresh‐cut lettuce.  相似文献   

12.
Ground liquorice roots were exposed to various doses (0, 5, 10, 15 and 20 kGy) of gamma radiation from a 60Co source. Irradiated and non‐irradiated samples were stored at room temperature. Microbial population, viscosity, concentrations of some minerals and the sensory properties of the extracts were evaluated after 0 and 12 months of storage. Tests carried out immediately after irradiation showed that the microbial count had been reduced and that the dose required to reduce the count by 1 log cycle (D10) was about 2 kGy. No effect was observed on the total dissolved solids in extracts of liquorice roots. Glycyrrhizinic acid concentration in the extracts and the viscosities of suspensions produced from irradiated roots were lower than those from non‐irradiated ones. Sensory evaluation indicated that there were no significant differences (P < 0.05) in colour, taste or flavour between extracts produced from irradiated and non‐irradiated roots. However, after 12 months of storage, some mineral ion (Na+, Ca2+ and K+) concentrations in extracts produced from irradiated roots were lower than in those from non‐irradiated ones; no significant differences (P < 0.05) in viscosity were found between suspensions of irradiated and non‐irradiated roots. © 2002 Society of Chemical Industry  相似文献   

13.
The effectiveness of radiation disinfestation of California peaches, nectarines and plums infested by Mediterranean fruit flies (Ceratitis capitata) and the effects of gamma-radiation up to 1 kGy (100 krad) on the sensory qualities of these fruits were studied. Medfly eggs in infested peaches and nectarines treated at 0.4–0.5 kGy (40–50 krad) in a Cobalt-60 irradiator did not hatch. Results in irradiated plums were inconclusive. Results of triangle tests with a taste panel of 15–20 show no differences in sensory qualities between plum and nectarine samples irradiated at 0.3 kGy and their controls. Some differences were detected between irradiated samples and controls: color and flavor of peaches at 0.3 kGy; color of plums and peaches 0.5 kGy; aroma of peaches and texture of plums at 0.5 kGy. At 1 kGy, differences were found in the texture of plums, nectarines, and peaches, and color and flavor of nectarines. Better control of harvesting, shipping and handling would help retain the qualities of these irradiated fruits.  相似文献   

14.
Effects of different packaging methods (air and modified atmosphere packaging) combined with irradiation (0.0, 1.0, and 2.0 kGy) on the preservation of saffron samples stored at room temperature for up to 60 days were investigated. Microbial analysis of aerobic bacteria, coliform, Escherisha coli, mold, and yeast was carried out. Among the analyzed bacteria, coliforms were most sensitive to γ-radiation. Based on sensory analyses and physicochemical analyses as a whole, the saffron samples packaged under modified atmosphere and irradiated with dose 2.0 kGy were acceptable under storage for 60 days, compared to 30 days for air-packaged non-irradiated samples.  相似文献   

15.
Abstract: Meatballs were prepared by mixing ground beef and spices and inoculated with E. coli O157:H7, L. monocytogenes, and S. enteritidis before packaged in modified atmosphere (3% O2+ 50% CO2+ 47% N2) or aerobic conditions. The packaged samples were irradiated at 0.75, 1.5, and 3 kGy doses and stored at 4 °C for 21 d. Survival of the pathogens, total plate count, lipid oxidation, color change, and sensory quality were analyzed during storage. Irradiation at 3 kGy inactivated all the inoculated (approximately 106 CFU/g) S. enteritidis and L. monocytogenes cells in the samples. The inoculated (approximately 106 CFU/g) E. coli O157:H7 cells were totally inactivated by 1.5 kGy irradiation. D10‐values for E. coli O157:H7, S. enteritidis, and L. monocytogenes were 0.24, 0.43, and 0.41 kGy in MAP and 0.22, 0.39, and 0.39 kGy in aerobic packages, respectively. Irradiation at 1.5 and 3 kGy resulted in 0.13 and 0.36 mg MDA/kg increase in 2‐thiobarbituric acid‐reactive substances (TBARS) reaching 1.02 and 1.49 MDA/kg, respectively, on day 1. Irradiation also caused significant loss of color and sensory quality in aerobic packages. However, MAP effectively inhibited the irradiation‐induced quality degradations during 21‐d storage. Thus, combining irradiation (3 kGy) and MAP (3% O2+ 50% CO2+ 47% N2) controlled the safety risk due to the potential pathogens and maintained qualities of meatballs during 21‐d refrigerated storage. Practical Application: Combined use of gamma irradiation and modified atmosphere packaging (MAP) can maintain quality and safety of seasoned ground beef (meatball). Seasoned ground beef can be irradiated at 3 kGy and packaged in MAP with 3% O2+ 50% CO2+ 47% N2 gas mixture in a high barrier packaging materials. These treatments can significantly decrease risk due to potential pathogens including E. coli O157:H7, L. monocytogenes, and S. enteritidis in the product. The MAP would reduce the undesirable effects of irradiation on quality, and extend the shelf life of the product for up to 21 d at 3 °C.  相似文献   

16.
Changes in cumin and chili powder from India resulting from electron‐beam irradiation were investigated using 3 analytical methods: electronic nose (E‐nose), Fourier transform infrared (FTIR) spectroscopy, and electron paramagnetic resonance (EPR) spectroscopy. The spices had been exposed to 6 to 14 kGy doses recommended for microbial decontamination. E‐nose measured a clear difference in flavor patterns of the irradiated spices in comparison with the nonirradiated samples. Principal component analysis further showed a dose‐dependent variation. FTIR spectra of the samples showed strong absorption bands at 3425, 3007 to 2854, and 1746 cm?1. However, both nonirradiated and irradiated spice samples had comparable patterns without any noteworthy changes in functional groups. EPR spectroscopy of the irradiated samples showed a radiation‐specific triplet signal at g = 2.006 with a hyper‐fine coupling constant of 3 mT confirming the results obtained with the E‐nose technique. Thus, E‐nose was found to be a potential tool to identify irradiated spices.  相似文献   

17.
Cetylpyridinium chloride (CPC) solutions (0, 0.5, or 1.0%) were applied to cantaloupe (“Athena” and “Hale's Best Jumbo” cultivars) rind plugs, either before or after inoculation with a broth culture of Salmonella Michigan (109 CFU/mL) and held at 37°C for 1 or 24 hr. Rind plugs were diluted, shaken, and sonicated, and solutions were enumerated. Texture quality and color were evaluated over 14 days storage at 4°C after 0 and 1% CPC spray applications. A 0.5 or 1.0% (vol/vol) application of CPC after Salmonella reduced the pathogen levels between 2.34 log CFU/mL and 5.16 log CFU/mL in comparison to the control (p < .01). No differences were observed in the firmness and color of 1% CPC treated cantaloupes. Salmonella concentrations on cantaloupes, treated with 1.0% CPC, were lower after 1 hr storage as compared to 24 hr. And, Salmonella on “Athena” surfaces were more susceptible to CPC spray treatments than on “Hale's Best Jumbo.”

Practical applications

Cetylpyridinium chloride (CPC) is the active ingredient of some antiseptic oral mouth rinses, and has a broad antimicrobial spectrum with a rapid bactericidal effect on gram‐positive pathogens. The spray application of CPC solutions to cantaloupe may reduce the level of Salmonella surface contamination during production from irrigation water and manure fertilizers and, during food processing by contaminated equipment and food handlers. Since the surfaces of cantaloupes are highly rough or irregular, bacteria can easily attach to these surfaces and become difficult to remove. Appropriate postharvest washing and sanitizing procedures are needed that can help control Salmonella and other pathogens on melons, especially on cantaloupes with nested surfaces. A direct surface spray application of CPC may be an alternative antimicrobial postharvest treatment to reduce pathogen contamination of cantaloupe melons, while providing an alternative to chlorine‐based solutions.  相似文献   

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The aim of this study was to determine the growth kinetics of Listeria monocytogenes, with and without cold‐adaption, on fresh‐cut cantaloupe under different storage temperatures. Fresh‐cut samples, spot inoculated with a 4‐strain cocktail of L. monocytogenes (~3.2 log CFU/g), were exposed to constant storage temperatures held at 10, 15, 20, 25, or 30 °C. All growth curves of L. monocytogenes were fitted to the Baranyi, modified Gompertz, and Huang models. Regardless of conditions under which cells grew, the time needed to reach 5 log CFU/g decreased with the elevated storage temperature. Experimental results showed that there were no significant differences (P > 0.05) in the maximum growth rate k (log CFU/g h?1) and lag phase duration λ (h) between the cultures of L. monocytogenes with or without previous cold‐adaption treatments. No distinct difference was observed in the growth pattern among 3 primary models at various storage temperatures. The growth curves of secondary modeling were fitted on an Arrhenius‐type model for describing the relationship between k and temperature of the L. monocytogenes on fresh‐cut cantaloupe from 10 to 30 °C. The root mean square error values of secondary models for non‐ and cold‐adapted cells were 0.018, 0.021, and 0.024, and 0.039, 0.026, and 0.017 at the modified Gompertz, Baranyi, and Huang model, respectively, indicating that these 3 models presented the good statistical fit. This study may provide valuable information to predict the growth of L. monocytogenes on fresh‐cut cantaloupes at different storage conditions.  相似文献   

19.
This study aimed at using essential oil (EO) alone or combined EO with nisin and γ‐irradiation to control Salmonella Typhimurium during the refrigerated storage of mini‐carrots. Peeled mini‐carrots were inoculated with S. Typhimurium at a final concentration of approximately 7 log CFU/g. Inoculated samples were coated by 5 different coating solutions: (i) nisin solution at final concentration of 103 IU/mL; (ii) mountain savory EO solution at 0.35%; (iii) carvacrol solution at 0.35%; (iv) mountain savory EO at 0.35% plus nisin solution of 103 IU/mL; or (v) carvacrol at 0.35% plus nisin solution of 103 IU/mL. Coated mini‐carrots were then irradiated at 0.5 or 1.0 kGy and compared to an unirradiated control sample. Samples were kept at 4 °C and microbial analyses were conducted at days 1, 3, 6, and 9. The results showed that mini‐carrots coated by carvacrol plus nisin solution or mountain savory EO plus nisin solution in combination with irradiation at 1.0 kGy completely eliminated S. Typhimurium to under the detection limit during the storage. Thus, the combined treatments using carvacrol plus nisin or mountain savory EO plus nisin coating solution and irradiation at 1.0 kGy could be used as an effective method for controlling S. Typhimurium in mini‐carrots.  相似文献   

20.
Abstract: Two varieties of green onions, Banner and Baja Verde, were inoculated with a cocktail of 3 Salmonella strains using dip and spot inoculation and irradiated at 0, 0.3, 0.6, 0.9, and 1.2 kGy using electron beam. Salmonella survivors were enumerated using a XLD underlay/TSAYE overlay plating method. The D values were in the range of 0.26 to 0.32 kGy depending on variety but not on the method of inoculation. This indicated that a 5‐log reduction of Salmonella can be achieved at a dose of 1.6 kGy. For the quality study, both varieties of green onions were irradiated at 0, 1.5, 2.0, and 2.5 kGy and evaluated for changes in microbial counts, color, texture, and visual quality during storage at 4 °C. Irradiation reduced total plate counts and psychrotrophs by 3 logs. Although the counts increased during storage, they did not exceed the initial counts of control. No significant difference was observed in color and texture between irradiated samples and control. The control maintained good visual quality for about 13 d as compared to 15 d for 1.5 and 2.5 kGy samples. The 2.0 kGy samples maintained good visual quality for 17 d suggesting that irradiation can increase shelf life by reducing spoilage microorganisms but higher doses can be detrimental to quality. At the dose levels required to achieve a 5‐log reduction in Salmonella, the shelf life of whole green onion can be extended. This study shows that irradiation can be used to enhance safety without adverse effects on quality.  相似文献   

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