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棉秆的微生物降解及糖化工艺研究 总被引:4,自引:0,他引:4
用2株放线菌J71、J74对经一定预处理的棉秆进行降解,首先检测了2株菌降解棉秆纤维的酶活动力学,结果表明,一定检测周期内,2株菌对棉秆有较强的降解作用,其酶活高峰期均出现在第6 d。以棉秆作为唯一碳源,研究了不同氮源:(NH4)2SO40.1%、0.2%、0.5%,尿素0.1%、0.2%、0.5%,10%麸皮汁对菌株产酶能力的影响。结果显示,以10%麸皮汁为氮源时,J71和J74降解棉秆的CMC酶活、FPA酶活及糖化率最高。通过正交试验检测了发酵影响因子棉秆粒径、发酵液起始pH、发酵温度和发酵时间对菌株J71和J74降解棉秆糖化率的影响,得出了棉秆糖化较优的工艺参数,即将棉秆制成10目粒径的颗粒,维持发酵液在pH 5.0~6.0,于37℃发酵9 d,能获得较高的糖化率。 相似文献
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中国曲中凝乳酶高产菌株的筛选及产酶条件的研究 总被引:2,自引:0,他引:2
目的:从中国曲中筛选凝乳酶高产茵株以代替小牛皱胃酶作凝乳剂.方法:采用酪蛋白平板进行初筛,测定茵株发酵产酶活力并进行复筛,研究其产酶发酵条件.结果:经多次复筛选育出1株高产凝乳酶活力茵株 Rhizopus arrhizus F34.碳源种类对该菌株产酶影响较大,无机氮源对产酶有抑制作用;MgSO4、FeSO4、ZnSO4对产酶也有较大的抑制作用,CaC12、KC1有利于凝乳酶的产生;pH、发酵温度和发酵时间对F34菌株产酶影响显著.结论:F34菌株产凝乳酶的最佳发酵培养基是:米粉麸皮水解液4.5%、豆粉水解液4.0%、CaC12 0.3%、KC10.5%;最适发酵条件:初始pH 4.5,发酵温度34℃,培养时间60 h. 相似文献
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高产蛋白酶菌株的筛选及产酶条件优化 总被引:1,自引:0,他引:1
从山西临汾汾河滩涂的表层土壤中分离到22株产蛋白酶菌株,用检测蛋白酶产生水解圈和蛋白酶活性测定相结合的方法,从中筛选出一株高产蛋白酶菌株P5,并通过正交试验对其产酶条件进行了研究。结果表明,影响P5菌株发酵产酶因素的主次顺序为培养温度、接种量、培养时间;影响P5菌株发酵培养基组成因素的主次顺序为氮源含量、起始pH值、碳源含量;确定的P5菌株的最佳产酶条件为,在以30 g/L葡萄糖为碳源,50 g/L蛋白胨为氮源,起始pH7.0的最适产酶培养基中,培养温度35℃,接种量为8%的条件下发酵培养48 h,具有最大产酶量,其蛋白酶活力可达129.2 U/mL。 相似文献
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提高漆酶活力的菌株组合筛选及诱导剂的研究 总被引:1,自引:0,他引:1
采用木质素降解酶互补的产酶组合培养和碱溶木质素降解相结合的方法,对实验室保存和分离的9株木质素降解菌株进行组合培养实验.结果表明,编号为m-6、55、m-8三株菌组合时,提高了整体产漆酶量,最高酶活达到了 891.7 U/L,比单菌株m-6的产酶皱提高了6倍.该组合菌的最适产酶pH值为5.5,最遁产酶温度为32℃.在对其产酶特性的研究中发现,外加藜芦醇5 mmoL/L、吐温-80 0.01%和愈创木酚3 mmoL/L,对组合菌株分泌漆酶有很大的促进作用,漆酶最高酶活达到2152.8 U/L,比对照样提高了166.3%.固态产酶培养后,第30天时,稻草粉的失重可达51.4%,纤维素降解率为38.5%,木质素的降解率为45.2%. 相似文献
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《食品与发酵工业》2017,(10):49-55
从菌株对桉叶残渣的降解、纯酶与桉叶木质素吸附和降解以及底物和产物变化3个角度,比较研究了木霉LaTr01和灵芝TR6产真菌漆酶降解桉叶木质素的差异。研究结果表明,残渣木质素的降解程度与菌株产漆酶的能力紧密相关,菌株在以桉叶残渣为底物的固态发酵过程中,灵芝TR6产漆酶酶活较高,但木霉LaTr01具有表达量大、胞外分泌率高、产酶速度快等特点;两种纯化真菌漆酶在精制桉叶木质素上的吸附更贴近非均匀表面两阶段多层吸附,符合Sips吸附模型,灵芝TR6被桉叶木质素吸附量较多,酶解效果就更明显;底物光谱分析和产物GC-MS法检测表明,两种漆酶对木质素降解后底物的官能团在种类上没有变化,数量上有一定的差异,降解途径都是侧链氧化去甲基化和芳香环骨架断裂。两真菌漆酶降解桉叶木质素的差异性主要体现分子结构导致的与底物易接近性,从而进一步导致降解酶解率、产物种类和数量的差异。 相似文献
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目的:筛选鉴定产几丁质酶的菌株并优化其发酵条件,最终应用于虾壳降解研究。方法:以盐城市滩涂海泥为样品,利用平板筛选水解几丁质的菌株,运用生物信息学方法鉴定菌株,通过单因素优化其发酵条件,并将筛选得到的菌株和优化后的发酵条件用于虾壳发酵。结果:鉴定得到三株显著降解胶体几丁质的菌,分别是发光杆菌(Photobacterium sp. LYM-1)、需钠弧菌(Vibrio sp. WM-1)和希瓦氏菌(Shewanella sp. ZXY-1);优化发酵条件:发光杆菌的碳源为几丁质10 g/L,氮源为NH4Cl 2.0 g/L,接种量为3%,发酵液pH为6.5,温度为32 ℃,发酵1 d酶活最高为15.37±0.55 U/mL,是优化前的4.37倍;需钠弧菌的碳源为几丁质10 g/L,氮源为NH4Cl 2.0 g/L,接种量为3%,发酵液pH为7.5,温度为22 ℃,发酵2 d酶活最高为40.82±6.03 U/mL,是优化前的1.60倍;希瓦氏菌的碳源为几丁质10 g/L,氮源为(NH4)2SO4 2.0 g/L,接种量为3%,发酵液pH为6.5,温度为22 ℃,发酵1 d酶活最高为25.64±3.29 U/mL,是优化前的2.47倍;三株菌均能利用虾壳产几丁质酶,但利用效率均低于几丁质,酶活力分别为10.25±0.95、32.16±2.25和21.81±4.27 U/mL。结论:本研究从盐碱地筛选得到三株产几丁质酶的菌株,优化后酶活力均得到提高,且均能利用虾壳产几丁质酶,为发酵虾壳制备几丁质酶提供新的菌株来源。 相似文献
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dos Santos Tamires Carvalho dos Santos Reis Nadabe Silva Tatielle Pereira Pereira Machado Fabiano de Paula Ferereira Bonomo Renata Cristina Franco Marcelo 《Food science and biotechnology》2016,25(1):205-211
Food Science and Biotechnology - Prickly palm cactus husk was used as a solid-state fermentation support-substrate for production of the ligninolytic enzymes laccase, peroxide manganese, and lignin... 相似文献
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刺芹侧耳木质素降解酶纯化及对染料脱色作用 总被引:1,自引:0,他引:1
对Pleurotus eryngii Co60-7菌株发酵液中木质素降解酶的分离纯化过程进行了研究,设计了一条主要由加盐沉淀和离子交换组成的分离纯化路线。通过35%硫酸铵饱和度的初沉和75%硫酸铵饱和度二沉可最大程度去除发酵液中的杂蛋白并获得目标蛋白,蛋白回收率为90.8%,纯化因子为3.81。用DEAE-SepharoseTMFast Flow进行三步洗脱层析,可较好地实现发酵液中目标蛋白的捕获。经过该纯化路线,木质素降解酶蛋白回收率达75.42%,纯化因子为10.55。经SDS-PAGE和Native-PAGE电泳分析,洗脱液DEⅡ中含有3种木质素降解酶组分。用该酶液进行溴酚蓝、亚甲基蓝和刚果红降解实验,结果表明该木质素降解酶液对3种染料都有不同程度的降解,染料间结构的差异会影响其降解效率。 相似文献
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C. M. Ajila F. Gassara Satinder Kaur Brar M. Verma R. D. Tyagi J. R. Valéro 《Food and Bioprocess Technology》2012,5(7):2697-2707
An investigation was carried out to understand the changes and mobilization of polyphenolics and the improvement in their antioxidant properties in apple pomace by solid-state fermentation using Generally Recognized as Safe grade fungus, Phanerochaete chrysosporium, by different fermentation techniques, such as flask, tray, and fermentor. ??-glucosidase, ligninolytic enzymes activity, and polyphenolic-linked antioxidant activity of apple pomace during solid-state fermentation was studied. During the course of solid-state fermentation, there was an increase in the extractable polyphenolic content (15.53 to 29.28?mg of gallic acid equivalents per gram of dry weight (DW)) on the 7th day followed by a decline in the polyphenol content. Antioxidant activity was measured by 1,1-diphenyl-2-picrylhydrazyl radical inhibition system, and the increase in activity (??35%) was directly proportional to polyphenolic content over the course of solid-state fermentation. After an initial lag phase with little activity, the ??-glucosidase activity increased by 6-, 7-, and 6-fold in flask (18.12?U/g DW samples), fermentor (44.52?U/g of DW sample), and tray fermentation (46.66?U/g DW sample) methods, respectively. Both polyphenolics and antioxidant capacity correlated with the increase in the ??-glucosidase activity and showed that the enzyme played an important role in the release of polyphenolic aglycones from apple pomace and therefore increased the antioxidant capacity. In addition, ligninolytic enzymes showed a direct correlation with the mobilization and polymerization of polyphenolic content during the solid-state fermentation. 相似文献
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以黄芪作为”药性基质”,在一定的条件控制下,运用双向发酵技术,经侧耳菌发酵,生成黄芪”药性菌质”,比较黄芪发酵后多糖成分及含量的变化。采用琼脂糖凝胶电泳法对黄芪发酵前后多糖进行定性鉴别,并用蒽酮-硫酸法测定其含量。黄芪发酵前后多糖的琼脂糖凝胶电泳呈一个均一斑点,迁移率分别为0.98和1.21。黄芪发酵前后多糖得率分别为6.31%和9.50%.含量分别为2.12%和3.64%。结果表明,琼脂糖凝胶电泳法可用于黄芪发酵后多糖的定性鉴别,黄芪经侧耳菌发酵后多糖提取率及含量均明显增加,充分体现了中药发酵可以使其活性成分较大限度释放的优点。 相似文献
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Fatma Gassara Chandran Matheyambath Ajila Satinder Kaur Brar Rajeshwar Dayal Tyagi Mausam Verma José Valero 《International Journal of Food Science & Technology》2013,48(10):2119-2126
The effect of different types of agitation (i) continuous agitation (C); (ii) continuous, discontinuous, continuous agitation (C/D/C); (iii) discontinuous, continuous, discontinuous agitation (D/C/D) and aeration (0.87, 1.25, 1.66 vvm) on ligninolytic enzyme production and polyphenolic compounds extraction by solid‐state cultures of Phanerochaete chrysosporium BKM‐F‐1767 was investigated. Higher production of manganese peroxidase (MnP) (1690.3 ± 87.6), lignin peroxidase (LiP) (387.9 ± 14.3) and laccase (898.9 ± 53.3 U gds?1) and liberation of total polyphenolics (ranging from 12.22 ± 1.1 to 30.12 ± 0.88 mg gallic acid equivalents per gram DW) was obtained after 195, 147, 219 and 204 h of fermentation, respectively, using 1.66 vvm as airflow and (C/D/C) agitation mode. Maximal enzyme production and total polyphenolic content were influenced by aeration, and higher values were obtained using 1.66 vvm as airflow rate, followed by 1.25 and 0.83 vvm, respectively. They were also influenced by agitation, and maximal values were obtained using C/D/C, followed by D/C/D and continuous agitation, respectively. The agitation modes influenced the production of ligninolytic enzymes and simultaneous extraction of polyphenols. 相似文献
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为降低生产成本、提高白灵菇产品的附加值,采用液体发酵法获得平菇发酵液,然后以此为基础与山楂浆混合,调配、研制复合饮料。研究结果表明:白灵菇液体培养最佳条件为:250mL锥型瓶摇瓶装液量为80mL,接种量为15%,pH值为6.5,摇瓶转速180r/min,在25℃培养8d。白灵菇山楂复合饮料的最佳配方为:发酵液添加量为20%,山楂浆添加量为15%,蔗糖添加量为10%,总酸含量为(以柠檬酸计)0.4%。 相似文献