食品酶制剂对冷冻面团流变学和微结构的影响

应用动态流变仪,Brabender拉伸仪,扫描电子显微镜(SEM)研究了葡萄糖氧化酶和谷氨酰胺转胺酶对冷冻面团粘弹模性量粘弹模量,抗拉伸阻力R5及微结构影响。空白面团(未加添加剂),含有葡萄糖氧化酶面团和含有谷氨酰胺转胺酶面团于-18℃冷冻贮藏7,21,35d,随冷冻贮藏时间延长,面团弹性模量(G′)降低。在同一冷冻贮藏时期内空白面团弹性模量最小,添加葡萄糖氧化酶面团弹性模量最大;含有葡萄糖氧化酶和含有谷氨酰胺转胺酶面团抗拉伸阻力R5大于空白面团。葡萄糖氧化酶和谷氨酰胺转胺酶使新鲜面团(未冷冻面团)面筋网络增强,淀粉颗粒镶嵌于交错的面筋网络之间,在-18℃经过35d冷冻贮藏,空白面团面筋网络不再连续,支离破碎,并与淀粉颗粒分离,而且面筋膜变薄。含有葡萄糖氧化酶和含有谷氨酰胺转胺酶面团依然有大量连续面筋网络存在。葡萄糖氧化酶和谷氨酰胺转胺酶抑制了面团弹性模量和抗拉伸阻力R5的恶化,而且抑制冰晶对面团中面筋三维网络结构的破坏。     

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食品酶制剂对冷冻面团流变学和微结构的影响应用动态流变仪,Brabender拉伸仪,扫描电子显微镜(SEM)研究了葡萄糖氧化酶和谷氨酰胺转胺酶对冷冻面团粘弹模性量粘弹模量,抗拉伸阻力R5     

酶在面包工业中应用

该文综述α-淀粉酶、葡萄糖氧化酶、木聚糖酶、谷氨酰胺转胺酶、脂肪氧合酶、脂肪酶和植酸酶作用原理及对面团流变性和面包品质影响。     

葡萄糖氧化酶和谷氨酰胺转胺酶在面制品中应用

该文综述葡萄糖氧化酶和谷氨酰胺转胺酶的特性、作用机理,及其对面团流变学特性和面制品品质影响。     

谷氨酰胺转氨酶对冷冻面团和面包品质的影响

对谷氨酰胺转氨酶在冷冻1 d、3 d面团中的应用进行了研究。结果如下:与对照相比,谷氨酰胺转氨酶对面团的发酵特性和面包品质改善作用明显;随着冻藏时间的延长,面团的发酵力、酵母存活率、面包比容、面包柔软度均呈下降趋势;冷冻1 d时,谷氨酰胺转氨酶添加量为0.8 g/kg时酵母存活率最大、酵母发酵力最强、面包比容和柔软度最好;冷冻3 d,对酵母存活率和面包比容的影响,最佳添加量为0.8 g/kg,对酵母发酵力的影响,最佳添加量为1.6 g/kg,对面包柔软度的影响为1.2 g/kg。     

复合酶制剂对面包品质影响

研究葡萄糖氧化酶(GOD)、转谷氨酰胺酶(TG)和脂肪酶(LIP)复配使用对面包硬度、弹性及水分迁移影响;结果表明,添加复合酶均能在面包贮藏期有效延缓面包老化、增强面包保持弹性能力、提高面包保持水分能力。其中,转谷氨酰胺酶30 ppm、脂肪酶20 ppm组合对延缓面包硬度增加效果最明显;葡萄糖氧化酶20 ppm、脂肪酶10 ppm组合对保持面包弹性能力最显著;葡萄糖氧化酶20 ppm、转谷氨酰胺酶30 ppm组合对保持面包水分作用最明显。     

酶在面包加工中的应用及研究现状

酶是一种高效、安全、纯天然的生物制品。针对酶对面团流变学特性、对面包感官品质和保藏老化以及对面包冷冻面团等方面的影响进行了综述,介绍了淀粉酶、木聚糖酶、谷氨酰胺转氨酶、脂肪酶、葡萄糖氧化酶、巯基氧化酶等在面包加工中的应用,为酶在面包加工中的进一步开发应用提供参考。     

冰结构蛋白影响冷冻面团及面包体系发酵烘焙与热力学特性的研究

研究了冰结构蛋白(ice structuring protein,ISP)作为新型冷冻食品添加剂在三种面团及面包体系,包括冷冻面团面包、预烘焙冷冻面包和新鲜面团面包中的应用,并探索了其对冷冻面团及面包体系发酵烘焙与热力学特性产生的影响。结果表明:ISP可增大冷冻面团面包的比容,经-18℃冻藏14d的ISP强化面团可得到与新鲜面团面包比容接近的结果;ISP有软化面包质地的作用,对经长时间冻藏的样品效果更显著;含有ISP的面包冻藏过程中水分含量保持相对稳定,而对照样增大趋势明显;ISP的引入致使面团体系糊化焓增大。     

酶制剂对面团流变学特性和面包品质的影响

探讨不同添加量的酶制剂对面团流变学特性和面包品质的影响。结果表明:真菌淀粉酶和木聚糖酶可弱化面团的流变学特性,葡萄糖氧化酶、谷氨酰胺转氨酶和脂肪酶可不同程度地改善面团的流变学特性;真菌淀粉酶、木聚糖酶、葡萄糖氧化酶和脂肪酶能有效改善面包焙烤品质,增大面包的比容,改善面包的硬度和弹性,而谷氨酰胺转氨酶对面包品质的影响不明显。真菌淀粉酶、木聚糖酶、葡萄糖氧化酶和脂肪酶的最适添加量分别是5μL/kg、20mg/kg、30μL/kg、50mg/kg。     

γ-聚谷氨酸对全麦冷冻面团烘焙特性的影响

将γ-聚谷氨酸(γ-PGA)添加至全麦冷冻面团中,通过全麦粉持水率、酵母存活率研究γ-PGA的保水性和抗冻活性;以面包比容和质构等作为评价指标,研究γ-PGA对冷冻面团制作的全麦面包烘焙品质的影响。结果表明,添加γ-PGA可提高冷冻全麦面团的酵母存活率和发酵高度,增大全麦面包比容,减小面包硬度,促进面包芯中形成大气孔;贮藏3 d后,添加γ-PGA的全麦面包硬度和老化率显著降低(P<0.05),且γ-PGA的最佳添加量为1%。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Chlorohydrins of bisphenol A diglycidyl ether (BADGE) and of bisphenol F diglycidyl ether (BFDGE) in canned foods and ready-to-drink coffees from the Japanese market

BADGE.2HCl and BFDGE.2HCl were determined in 28 samples of ready-to-drink canned coffee and 18 samples of canned vegetables (10 corn, 5 tomatoes and 3 others), all from the Japanese market. HPLC was used as the principal analytical method and GCMS for confirmation of relevant LC fractions. BADGE.2HCl was found to be present in one canned coffee and five samples of corn, BFDGE.2HCl in four samples of canned tomatoes and in one canned corn. No sample was found which exceeded the 1mg/kg limit of the EU for the BADGE chlorohydrins. However the highest concentration was found for the sum of BFDGE.2HCl and BFDGE.HCl.H₂O at a level of 1.5mg/kg. A Beilstein test confirmed that all cans containing foods contaminated with BADGE.2HCl or BFDGE.2HCl had at lest one part coated with a PVC organosol.     

European priorities for research to support legislation in the area of food contact materials and articles

A strong science base is required to underpin the planning and decision-making process involved in determining future European community legislation on materials and articles in contact with food. Significant progress has been made in the past 5 years in European funded work in this area, with many developments contributing to a much better understanding of the migration process, and better and simpler approaches to food control. In this paper this progress is reviewed against previously identified work-areas (identified in 1994) and conclusions are reached about future requirements for R&D to support legislation on food contact materials and articles over the next 5 or so years.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the