温度响应型分子印迹水凝胶的应用研究进展

分子印迹是人工制备对某一特定分子具有选择性识别能力的聚合物的技术。将分子印迹与具有温敏性质的水凝胶相复合得到的温敏印迹材料,可通过外界温度变化自发的调节对模板分子的吸附与分离。综述了近年来温敏印迹材料在生物蛋白分离、金属离子吸附、电化学传感器、光催化降解、药物释放等方面的应用,并对温敏印迹技术未来的发展进行了展望。     

智能分子印迹水凝胶的研究进展

分子印迹技术具有预定性、识别性和实用性的特点,而智能水凝胶在外界环境刺激下能产生体积或相的转变。主要介绍了将2者结合起来的一类智能分子印迹水凝胶。它是一种能够实现环境调变响应的目标分子吸收与释放功能的高分子聚合物,其中环境变化主要包括温度、pH值、电刺激等。     

温敏型水凝胶的制备及溶胀特性

采用N-异丙基丙烯酰胺(NIPA)与N-乙烯基-2-吡咯烷酮(NVP)为共聚单体,以N,N-亚甲基双丙烯酰胺(BIS)为交联剂,过硫酸钾(KPS)为引发剂,通过化学交联的方法在水溶液中制备出P(NIPA-co-NVP)共聚物水凝胶。分别探讨了单体配比、交联剂用量等实验条件对水凝胶的温敏特性和溶胀性能的影响。利用傅里叶红外(FT-IR)对共聚物水凝胶的结构进行了表征。通过实验可知:当交联剂BIS和引发剂KPS分别为单体用量的5%和0.8%,制备的水凝胶具有较高的转化率、较好的机械强度和共聚性质。共聚物水凝胶中NVP含量越高,溶胀率越大,升温时溶胀率下降程度越大,自然条件下脱水速率越快;BIS用量越大,溶胀率越小,保水率越高,需要更长时间达到溶胀平衡。     

分子印迹模板分子类型研究进展

综述了模板分子的选择概况,包括有机小分子、生物大分子、细胞和病毒、无机离子和虚拟模板分子,并对模板分子的局限性做了详细介绍,以期对分子印迹技术的发展提供一些参考。     

聚N-异丙基丙烯酰胺水凝胶研究进展

简要阐述了聚N-异丙基丙烯酰胺(简称PNIPAM)水凝胶的制备方法、性能特点、应用等方面的研究进展。利用PNIPAM特有的温敏性特点,可以将PNIPAM与其他有机物复合,改善了其机械强度和温度敏感性,大大提高了PNIPAM的响应速度和溶胀率。制备的高分子材料在生物医学、免疫分析、分离提纯、蓄热等领域都有广泛的应用。     

分子印迹技术研究进展

分子印迹技术作为一种新型的亲和分离技术,是当前发展高选择性材料的主要技术之一。利用该技术所制备的分子印迹聚合物具有分子特异识别功能。本文主要介绍分子印迹技术的方法、基本原理、分子印迹聚合物的制备方法及其在食品分析、环境分析和药物分析中的应用,并对以后的发展方向提出了展望。     

表面分子印迹材料制备研究进展

表面分子印迹技术是通过把分子识别位点建立在印迹材料的表面,来提高识别位点与印迹分子的结合速度,进一步加强印迹材料吸附分离效率。从无机硅胶材料和聚合物材料两方面综述了表面分子印迹材料制备技术发展现状,着重评述了表面分子印迹聚合物微球的制备方法及相应产品的性能,并指出表面分子印迹技术存在的不足。     

分子印迹技术研究进展

分子印迹技术是近些年来快速发展的一种高选择性分离及分子识别技术,由该技术制备的具有分子特异识别功能的高分子材料称为分子印迹聚合物。本文就其基本原理、方法及其在天然产物分离、食品检测、仿生传感、固相萃取、抗体与受体模拟领域的应用与研究进行综述。     

分子印迹技术最新研究进展

随着分子印迹技术的不断发展,越来越多的新型分子印迹聚合物出现在人们的视野中。重点介绍了几种新型分子印迹聚合物,并且展望了分子印迹技术的未来。     

一种可温度与pH调控的分子印迹光催化材料的制备及其性能

以氧氟沙星为模板分子,通过表面引发原子转移自由基聚合技术（SI-ATRP）,成功合成了一种智能凝胶接枝纳米TiO₂的分子印迹光催化材料（TiO₂-DR/MIPs）。采用透射电镜（TEM）、红外光谱（FTIR）、热重（TG）和X射线衍射（XRD）等方法对该材料进行了形貌及性质表征。分别研究了材料的温度与pH敏感性质及其对氧氟沙星光催化降解效率的影响,并对该材料的选择性和稳定性进行了探究。结果表明,TiO₂-DR/MIPs的粒径可随着温度与pH的变化呈现出良好的响应特性,并且可以通过改变环境温度和pH实现对其光催化活性的调控。TiO₂-DR/MIPs对氧氟沙星具有较好的选择性吸附和降解作用,且稳定性和重复使用效果好,经4次光催化降解循环实验后,光催化效率仅降低了5％。此外,TiO₂-DR/MIPs中的双敏感分子印迹层还可以有效地阻止纳米TiO₂的团聚,实现纳米TiO₂的均匀分散。     

辛弗林分子印迹聚合物的制备及吸附性能评价

利用分子印迹技术,以辛弗林(SYN)为模板分子,甲基丙烯酸(MAA)为功能单体,二甲基乙二醇丙烯酸酯(EDMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,在四氢呋喃(THF)溶液中制备了辛弗林分子印迹聚合物(SYN-MIP);通过紫外光谱法研究了SYN与MAA的非共价作用,并通过静态平衡实验评价了SYN-MIP的吸附...     

硫丹分子印迹聚合物的合成及吸附特性分析

采用分子印迹技术,以α-甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为引发剂,乙腈为致孔剂,合成以硫丹为模板的印迹聚合物微球。首先利用紫外光谱方法研究了模板与功能单体相互作用情况,采用平衡吸附试验对印迹聚合物吸附效率进行了表征,与化学组成相同的空白聚合物相比,印迹聚合物微球对模板分子具有更高的吸附效率,按最佳合成条件得到的印迹聚合物最大表观吸附量达42.56 mmol/g。     

Preparation of molecularly imprinted polymer films on monodisperse macromolecular beads

A new and facile technique for preparing homogeneous and highly selective molecularly imprinted polymers (MIPs) films on porous monodisperse poly‐(glycidyl methacrylate‐co‐ethylene glycol dimethacrylate) beads (P_GMA‐EDMA beads) has been described: before polymerization, initiator was immobilized on the P_GMA‐EDMA beads' surface by chemical reactivity; then functional monomers and crosslinkers were initiated by the surface‐bound initiator and copolymerized to form MIPs films on the P_GMA‐EDMA beads' surface in the presence of template molecules. The resulting beads were analyzed by FTIR spectroscopy and X‐ray photoelectron spectra. The effects of the initiator amount and copolymerization time on the content of MIPs, and MIPs contents on morphologies and pore properties of the resulting beads were investigated. The results show that the content of MIPs can be adjusted by changing initiator amount or by controlling copolymerization time. The binding experiments indicate that, at lower grafting level, with the increase of MIPs content, the adsorption and recognition capabilities of the resulting beads enhance. When MIPs content increase to 16.75%, the highest adsorption and recognition capabilities are obtained. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009     

2,4-Dichlorophenol molecularly imprinted two-dimensional photonic crystal hydrogels

A novel molecularly imprinted two-dimensional (2-D) photonic crystal hydrogels (MIPH) for sensitive and label-free recognition of 2,4-dichlorophenol (2,4-DCP) was prepared. The 2-D photonic crystal template was fabricated by using air-water interface self-assembly method. And then the template was embedded with molecularly imprinted polymer, which was synthesized with 2,4-DCP as imprinted molecules, dimethyl sulfoxide as solvent, acrylic acid and acrylamide as functional monomers, N,N-methylene bis acrylamide as cross-linker, azobisisobutyronitrile as initiator. The imprinted molecules were removed by 0.01 M ammonia solution. The results indicated that the 2,4-DCP molecularly imprinted 2-D photonic crystal hydrogels has good response and recognition ability to 2,4-DCP. When the molar ratio of cross-linking density of MIPH is 2.3% and the molar ratio of imprinting molecule is 5.0%, the change of Debye ring diameter is the largest. The diameter of Debye ring increased by 7.1 mm when the concentration of 2,4-DCP changed from 0 to 1 × 10⁻⁶ M, and the particle spacing of MIPH reduced 38 nm. In addition, the diameter of the Debye ring hardly changed in the solution of analogues of 2,4-DCP such as, phenol, 2-chlorophenol, 2,4,6-trichlorophenol and so on, indicating that the MIPH has highly sensitivity and specificity.     

Preparation of water‐compatible molecularly imprinted polymers for caffeine with a novel ionic liquid as a functional monomer

Water‐compatible molecularly imprinted polymers (MIPs) for caffeine were synthesized in aqueous medium with a new functional monomer, 1‐(α‐methyl acrylate)‐3‐methylimidazolium bromide (1‐MA‐3MI‐Br), which had π–π and hydrogen‐bonding interactions. Caffeine‐imprinted polymers were prepared in suspension polymerization with 1‐MA‐3MI‐Br and methacrylic acid (MAA), which only had hydrogen bonding, as a functional monomer. For the specific binding characteristics of the new functional monomer 1‐MA‐3MI‐Br, the adsorption capacity and relative separation factor (β) of MIPs for caffeine were significantly enhanced. The maximum adsorption capacities of 1‐MA‐3MI‐Br–MIP and MAA–MIP imprinted microspheres for caffeine were 53.80 and 28.90 μmol/g, respectively. Moreover, the relative separation factors were measured by comparison of the separation characteristics under competitive adsorption conditions. The results showed that the β of MAA–MIP for caffeine relative to theophylline was only 1.65; this showed a very poor recognition selectivity for caffeine, but β of 1‐MA‐3MI‐Br–MIP for caffeine with respect to theophylline was remarkably enhanced to 3.19; this showed an excellent recognition selectivity and binding affinity toward caffeine molecules in an aqueous environment. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013     

分子印迹技术制备与应用进展

综述了分子印迹技术的制备方法及在色谱分离、固相萃取、膜分离、免疫分析、仿生传感、酶催化模拟等领域的应用。并指出对于分子印迹和识别的机理、在水相中实现分子印迹识别和大分子印迹聚合物的合成等方面需进一步研究。     

杨梅黄素分子印迹聚合物的制备及其识别性能研究

以杨梅黄素(MYR)为模板分子、2-乙烯基吡啶(2-VP)为功能单体、二甲基乙二醇丙烯酸酯(EDMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,甲醇为致孔剂,采用分子印迹技术制备了杨梅黄素分子印迹聚合物(MYR-MIP)。通过红外光谱、扫描电镜、热重分析等技术表征了MYR-MIP的理化性能;采用紫外光谱分析研究了MYR-MIP对MYR的等温吸附和动力学吸附特性,研究结果表明MYR-MIP对MYR具有良好的特异性吸附,并且在2 h内达到平衡吸附;高效液相色谱分析表明MYR-MIP对MYR具有优良的选择识别特性。     

Studies on the preparation and properties of inorganic molecularly imprinted polymer (MIP) based on tetraethoxysilane and silane coupling agents

Inorganic molecularly imprinted polymer (MIP) based on tetraethoxysilane (TEOS), methyl triethoxysilane (MTEOS), and phenyl triethoxysilane (PTEOS) by sol‐gel process has been developed. The MIP's preparation conditions, H₂O/Si molar ratios (R), template removal procedures, calcination temperatures, and quantity of ammonium hydroxide were investigated. The competition experiments of the MIP for template (caffeine, CAF) and analogue (theophylline, TH) were analyzed by high‐performance liquid chromatography (HPLC). The results showed that the Ad_CAF decreased with an increase of the H₂O/Si molar ratios, but the selectivity (α) increased with an increase of the H₂O/Si molar ratios in the MIP. In addition, in a comparison of the procedures for removing the template, calcination obtained better efficiency and higher selectivity than extraction. The optimum adsorption and selectivity of MIP were obtained with R = 10 and the template was removed by calcination at 600°C. Moreover, the selectivity of the MIP (283.9) was greater than the nonimprinted polymer (2.45) under optimum preparation conditions. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009     

Preparation of molecularly imprinted polymers in supercritical carbon dioxide

Molecularly imprinted polymer nanoparticles were prepared in supercritical carbon dioxide using a noncovalent imprinting approach. In the present work, propranolol was used as a model template, methacrylic acid as a functional monomer, and divinylbenzene as a crosslinker. Under a high dilution condition, the heterogeneous polymerization resulted in discrete crosslinked polymer nanoparticles. Compared with the nonimprinted polymers, the imprinted nanoparticles displayed much higher propranolol uptake in a low polarity organic solvent. The use of a single enantiomer (S)‐propranolol as the template clearly demonstrated that the imprinted binding sites are chiral‐selective, with a cross‐reactivity towards (R)‐propranolol of less than 5%. The overall binding performance of the imprinted nanoparticles was comparable to imprinted polymers prepared in conventional organic solvents. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 102: 2863–2867, 2006     

Synthesis and evaluation of a beta cyclodextrin-based molecularly imprinted copolymer

A new molecularly imprinted copolymer of 2-hydroxy ethyl methacrylate (HEMA) and beta-cyclodextrin-coupled HEMA is synthesized. The copolymer is studied for its interaction with a pair of steroids, namely, cholesterol and testosterone. The molecularly imprinted copolymer is found to absorb the print molecule by several fold compared to an imprinted poly (HEMA). The enhanced absorption capacity is attributed to the presence of beta cyclodextrin moieties in the copolymer. The imprinted copolymer could be used as an adsorbent matrix. © 1998 John Wiley & Sons, Inc. J Appl Polym Sci 70: 15–18, 1998