共查询到20条相似文献,搜索用时 15 毫秒
1.
D'Angelo L De Girolamo P Cellerino A Tozzini ET Varricchio E Castaldo L Lucini C 《Microscopy research and technique》2012,75(4):441-447
The S100 protein in nervous tissue appears to play important roles in regulating neuronal differentiation, glial proliferation, plasticity, development, axonal growth, and in neurogenetic processes. In fish, the adult neurogenic activity is much higher than in mammals. In this study, the localization of S100 protein was investigated in the brain of annual teleost fish, Nothobranchius furzeri, which is an emerging model organism for aging research. By immunohistochemical techniques, S100 immunoreactivity (IR) was detected in glial cells, small neurons, and fibers throughout all regions of central nervous system (CNS) with different pattern of distribution. In the telencephalon, S100 IR was seen in the olfactory bulbs and in different areas of the telencephalic hemispheres. In the diencephalon, S100 positivity was observed in the habenular nuclei of the epithalamus, in the cortical thalamic nucleus, in the dorsal, ventral and caudal portions, the latter with the posterior recessus nucleus, and in the diffuse inferior lobe of the hypothalamus, along the diencephalic ventricle and in the dorsal optic tract. In the mesencephalon, S100 IR was observed in the longitudinal tori, in the optic tectum, and along the mesencephalic ventricle. In the rhombencephalon, S100 IR was shown in valvula and body of the cerebellum, and in some nuclei of the medulla oblongata. The results suggest that S100 may play a key role in the maintenance of the CNS and in neurogenesis processes in the adulthood. 相似文献
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高分辨率成像二元光学系统的研制与实验结果 总被引:7,自引:0,他引:7
已研制成功一个折衍射混合式成像二元光学系统,焦距800mm,有效通光口径76mm,成像光谱范围从435.8nm到656.3nm.成像质量实测结果为20lp/mm时MTF值达到0.75,比同样条件下一个传统的折射式系统MTF所能达到的设计值高百分之三十以上。 相似文献
3.
Cantarero Carmona I Luesma Bartolomé MJ Lavoie-Gagnon C Junquera Escribano C 《Microscopy research and technique》2011,74(2):148-152
The intermediate nestin filament is expressed in neural stem cells, neuroectodermal tumors and various adult tissues under situations that reproduce developmental phases, e.g., physiological renewal of certain cell types, tissue regeneration, and healing or revascularization. In the human gastrointestinal tract, nestin has been reported in glial cells and interstitial cells of Cajal. We examined by immunohistochemistry the appearance and distribution of nestin protein in enteric ganglia of rat duodenum. Through the myenteric and submucosal plexuses, a high number of nestin-positive cells were visualized in this specie. The nestin-positive cells were smaller and more numerous than enteric neurons. They were present both within and around ganglia. The results of this study suggest that the rat enteric glial cells (EGCs) are rich in nestin, a protein usually associated with dividing or migrating cells and the dynamic reorganization of nestin filaments during the cell cycle. EGCs could function as enteric stem cells. 相似文献
4.
F. Abadía-Fenoll M. V. Ostos Garrido J. L. Caballero Lpez-Lendínez R. Carmona Martos 《Journal of microscopy》1985,137(3):225-232
This paper describes a block silver impregnation technique for the CNS. The procedure, which is quite simple, yields highly consistent and reproducible results. After fixation during 6–10 days in 10% saline formaldehyde, 4 mm thick blocks of brain are treated with chromic anhydride and sodium potassium tartrate solution for 4 days. After this period the specimens are rinsed in 0.75% silver nitrate solution to which 8–10 drops of pyridine per 100 ml of solution have been added. This is followed by impregnation for 4 days at 37°C in silver nitrate-pyridine solution identical to that used in the previous rinsing step. The impregnated blocks are reduced during 20–26 h in 1% pyrogallol to which 6 ml commercial formaldehyde per 100 ml of solution have been added, followed by dehydration in dioxan and paraffin embedding. Sections no thicker than 30 μm are then cut for histological study. This fundamentally neurofibrillar method reveals: (a) neuronal somata and their processes; (b) synaptic structures; (c) fibre bundles; and (d) cell nuclei and nucleoli. 相似文献
5.
根据国内仓库监控的现状,设计并实现了一款基于嵌入式Linux的仓库监控系统。其前端以嵌入式S5PC100为控制核心,在对硬件和软件进行了总体设计之后,重点研究并实现了TCP/IP协议网络下的嵌入式前端TCP方式的温湿度及控制信号传输和UDP方式的视频传输,实现了基于Video4Linux(V4L)技术对视频图像信息的采集。经测试,系统稳定可靠、成本低、可扩展性强,在其他监控领域也有一定的参考价值。 相似文献
6.
Sara M. M. El-Desoky;Ruwaida Elhanbaly;Abdalla Hifny;Nagwa Ibrahim;Wafaa Gaber; 《Microscopy research and technique》2024,87(4):774-789
The retina consists of various cell types arranged in eight cell layers and two membranes that originate from the neuroectodermal cells. In this study, the timing of differentiation and distribution of the cellular components and the layers of the rabbit retina are investigated using light and electron microscopy and immunohistochemical techniques. There were 32 rabbit embryos and 12 rabbits used. The rabbit retina begins its prenatal development on the 10th day of gestation in the form of optic cup. The process of neuro- and gliogenesis occurs in several stages: In the first stage, the ganglionic cells are differentiated at the 15th day. The second stage includes the differentiation of Muller, amacrine, and cone cells on the 23rd day. The differentiation of bipolar, horizontal, and rod cells and formation of the inner segments of the photoreceptors consider the late stage that occurs by the 27th and 30th day of gestation. On the first week of age postnatally, the outer segments of the photoreceptors are developed. S100 protein is expressed by the Muller cells and its processes that traverse the retina from the outer to the inner limiting membranes. Calretinin is intensely labeled within the amacrine and displaced amacrine cells. Ganglionic cells exhibited moderate immunoreactivity for calretinin confined to their cytoplasm and dendrites. In conclusion, all stages of neuro- and gliogenesis of the rabbit retina occur during the embryonic period. Then, the retina continues its development postnatally by formation of the photoreceptor outer segments and all layers of the retina become established. 相似文献
7.
Hannestad J Monjil DF Díaz-Esnal B Cobo J Vega JA 《Microscopy research and technique》2004,63(2):94-101
The immune system, especially the thymus, undergoes age-related modifications leading to structural and functional changes in the lymphoid organs and immunocompetent cells. Nevertheless, the consequences of thymic involution in the peripheral pool of T-cells are still a matter of controversy. The control of the thymic function is very complex and involves intrathymic signals, the autonomic nervous system, and the endocrine system. Both thymocytes and thymic stromal cells express receptors for a wide range of hormones, as well as for neurotransmitters and neuropeptides, thus affecting thymocytes maturation. This review summarizes the age-dependent variations in the extrathymic components of the thymic microenvironment, i.e., vegetative nerves and hormones, and the possible effects of those changes in the immune function. 相似文献
8.
视网膜细胞显微镜的照明系统 总被引:1,自引:0,他引:1
为满足视网膜细胞成像照明光束的高亮度、窄谱宽要求,提出了一种用于视网膜细胞成像的照明系统,将半导体激光器发出的632.8nm激光耦合到芯径为105μm的多模光纤中,用聚光镜将多模光纤的出光端汇聚到一个旋转毛玻璃上,再用一个投射物镜将毛玻璃上的光源像投射到眼睛里照亮视网膜。试验发现该照明方法很好地消除了激光散斑,满足视网膜显微成像对高亮度光源的要求,光源有较窄的谱宽,成像系统色差很小,成像质量优于采用传统氙灯做照明光源的图像。该照明系统能较好地满足视网膜显微成像。 相似文献
9.
尽管可以采用光折变技术制备硫化砷条波导,但工艺参数控制复杂,且传输损耗较大.为了解决这一问题,提出了一种用于光阻断效应的新型B270—As2S8复合光波导结构.该设计根据光波导模式耦合的基本原理,借助波束传播法(BPM)仿真软件,主要通过对离子交换波导交换时间和As2S8薄膜厚度的匹配设计,实现了632.8nm波段导波模场分布集中在As2 S8薄膜中传输,并始终维持单模特性.这个特点为有效实现光阻断效应提供了条件,非常具有实用价值. 相似文献
10.
Receptor binding occurrence and plasma levels of natriuretic peptides in response to sympathectomy 总被引:1,自引:0,他引:1
Hansson M 《Microscopy research and technique》2005,67(2):90-99
In the present investigation the relationship between the sympathetic nervous system and the endocardial levels of receptor binding sites for natriuretic peptides and the plasma content of atrial natriuretic peptide were analyzed in rats. In order to destruct the cardiac sympathetic nerve terminals, chemical sympathectomy with 6-hydroxydopamine was made in parallel with intravenous measurements of blood pressure and heart frequency. By use of immunohistochemical and enzyme-linked-immunosorbent techniques the expression of tyrosine hydroxylase-positive sympathetic nerve terminals and plasma levels of pro-atrial natriuretic peptide were determined, respectively. The occurrence of receptor binding sites for natriuretic peptides was examined by in vitro receptor autoradiography. In contrast to the marked occurrence of natriuretic peptide receptor binding sites seen in the ventricular endocardium of control rats, the sympathectomized rats exhibited a decreased number of binding sites for natriuretic peptides in the endocardium of both the right and left chambers. Interestingly, this was found in parallel with a significant decrease of systolic and diastolic blood pressure and increased plasma levels of pro-atrial natriuretic peptide in the treated group of rats. These findings, together with those in previous studies, give support to an idea that one part of the blood pressure-decreasing effects, seen in patients treated with beta-adrenergic blockade, might be through a reduction of the natriuretic clearance receptor C, then giving rise to increased levels of atrial natriuretic peptide. 相似文献
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Gallus L Ferrando S Bottaro M Diaspro A Girosi L Faimali M Ramoino P Tagliafierro G 《Microscopy research and technique》2009,72(2):101-109
The presence and distribution of FMRFamide-like peptides (FLPs) in the cyprid larvae of the barnacle Balanus amphitrite were investigated using immunohistochemical methods. Barnacles are considered to be one of the most important constituents of animal fouling communities, and the cyprid stage is specialized for settlement and metamorphosis in to the sessile adult condition. FLPs immunoreactive (IR) neuronal cell bodies were detected in both the central and the peripheral nervous system. One bilateral group of neurons somata was immunodetected in the brain, and IR nerve fibers were observed in the neuropil area and optic lobes. Intense immunostaining was also observed in the frontal filament complex: frontal filament tracts leaving the optic lobes and projecting towards the compound eyes, swollen nerve endings in the frontal filament vesicles, and thin nerve endings in the external frontal filament. Thin IR nerve fibers were also present in the cement glands. Two pairs of neuronal cell bodies were immunodetected in the posterior ganglion; some of their axons appear to project to the cirri. FLPs IR neuronal cell bodies were also localized in the wall of the dilated midgut and in the narrow hindgut; their processes surround the gut wall and allow gut neurons to synapse with one another. Our data demonstrated the presence of FLPs IR substances in the barnacle cyprid. We hypothesize that these peptides act as integrators in the central nervous system, perform neuromuscular functions for thoracic limbs, trigger intestinal movements and, at the level of the frontal filament, play a neurosecretory role. 相似文献
13.
The effect of nerve growth factor (NGF) on ontogenesis of frog mast cells was investigated in vivo by histochemical, morphometric, and ultrastructural analysis. Three groups of tadpoles at various stages of development were used. In the first group, the larvae received i.p. injections of 1 ng NGF/g; the second group received 10 ng NGF/g, while the control group received only the vehicle. The first recognizable mast cells arose symmetrically in the tongue at stage 26 of Witschi's standard table. At stages 26 and 29, the mast cell number in the NGF-injected tadpoles was significantly higher than the control group. From stage 29 onward, the mast cell number rapidly increased in all groups. No significant differences in mast cell number were observed between the control group and the NGF-injected groups at stages 31 and 33. Electron microscopy revealed that at metamorphic climax (stage 33), the mast cells in the NGF-treated groups were more mature than those in the control group. Therefore, nerve growth factor at early stages of tadpole development is likely to induce differentiation of mast cell precursors, while at later stages it is likely to induce maturation of immature mast cells. The close anatomical association between mast cells and perineurium, observed during nerve development, is intriguing. Already in the early stages of nerve development, the mast cells form a network around Schwann cell-axon complexes, together with the perineurial cells. At climax, the mast cells are located between the perineurial layers, suggesting that they may play a role in the tissue-nerve barrier of the perineurium. Nerve growth factor also seems to induce perineurial cell maturation. 相似文献
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Corbo CP Othman NA Gutkin MC Alonso Adel C Fulop ZL 《Microscopy research and technique》2012,75(3):325-333
Cellular composition of the adult zebrafish (Danio rerio) optic tectal cortex was examined in this study. Morphological techniques such as 1 μm thick serial plastic sections stained with osmium tetroxide and toluidine blue, modified rapid Golgi silver impregnation, GFAP immunohistochemistry, confocal microscopy, as well as scanning and transmission electron microscopy were used. Neuronal and glial components are described and the layers of the cortex are revisited. Specific neuronal arrangements as well as unique glial/ependymal cells are described. A three dimensional rendering of the astrocytic fiber arrangement in the marginal zone is presented and a composite drawing summarizes the cellular composition of the optic tectum. 相似文献
16.
李艳红 《机械工程与自动化》2012,(2):131-132
为了使仪表能够进行高、低温试验,设计了一个以SAM7X256128为主芯片植入linux操作系统的温度控制系统。采用热电阻Pt100进行温度采集,控制方式采用位式控制和PWM控制,具有时钟可自动计时、系统自动报警和自动断电的保护措施。此装置还与PC机相连,方便用户观察和记录数据。 相似文献
17.
Alan Hugh David Watson Friedrich-Wilhelm Schürmann 《Microscopy research and technique》2002,56(3):210-226
The Orthopteran central nervous system has proved a fertile substrate for combined morphological and physiological studies of identified neurons. Electron microscopy reveals two major types of synaptic contacts between nerve fibres: chemical synapses (which predominate) and electrotonic (gap) junctions. The chemical synapses are characterized by a structural asymmetry between the pre- and postsynaptic electron dense paramembranous structures. The postsynaptic paramembranous density defines the extent of a synaptic contact that varies according to synaptic type and location in single identified neurons. Synaptic bars are the most prominent presynaptic element at both monadic and dyadic (divergent) synapses. These are associated with small electron lucent synaptic vesicles in neurons that are cholinergic or glutamatergic (round vesicles) or GABAergic (pleomorphic vesicles). Dense core vesicles of different sizes are indicative of the presence of peptide or amine transmitters. Synapses are mostly found on small-diameter neuropilar branches and the number of synaptic contacts constituting a single physiological synapse ranges from a few tens to several thousand depending on the neurones involved. Some principles of synaptic circuitry can be deduced from the analysis of highly ordered brain neuropiles. With the light microscope, synaptic location can be inferred from the distribution of the presynaptic protein synapsin I. In the ventral nerve cord, identified neurons that are components of circuits subserving known behaviours, have been studied using electrophysiology in combination with light and electron microscopy and immunocytochemistry of neuroactive compounds. This has allowed the synaptic distribution of the major classes of neurone in the ventral nerve cord to be analysed within a functional context. 相似文献
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阐述了矿山安全监察系统的设计与实现,系统采用C/S和B/S混合模式,在各监察分局内使用C/S模式,方便数据的传输,保证内部信息的高度安全及信息传输和处理的高效;在不同的监察分局之间对系统的访问和信息交互则采用B/S模式,这样可充分利用INTERNET以降低成本,实现远程的信息传输和查询分析。并根据用户需求生成不同的显示界面,从而实现数据的动态浏览显示。 相似文献
20.
Classical electron microscopic morphological studies provide detailed ultrastructural information, which may lend insights into cellular functions. As a follow‐up to our morphological investigation of the adult zebrafish (Danio rerio) optic tectum, in this study, we have analyzed the ependymal structures lining the surfaces of the tectal ventricle: the torus, tegmental surface of the valvula cerebelli and the periventricular gray zone of the optic tectal cortex. We used toluidine blue stained plastic (semithin) sections for light microscopy and scanning electron microscopy. Our morphological findings of gated entrances and/or egresses indicate that, at least in the adult zebrafish brain, there may be a bidirectional direct flow communication between the ventricular cerebrospinal fluid and the parenchymal interstitial fluid. 相似文献