共查询到20条相似文献,搜索用时 46 毫秒
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P Webb P Nguyen J Shinsako C Anderson W Feng MP Nguyen D Chen SM Huang S Subramanian E McKinerney BS Katzenellenbogen MR Stallcup PJ Kushner 《Canadian Metallurgical Quarterly》1998,12(10):1605-1618
Estrogen receptor-alpha contains two transactivation functions, a weak constitutive activation function (AF-1) and a hormone-dependent activation function (AF-2). AF-2 works by recruiting a large coactivator complex, composed of one or more p160s, CREB-binding protein (CBP)/p300, and P/CAF (p300 and CBP-associated factor), via direct contacts with the p160s. We report here that independent AF-1 activity also requires p160 contacts. Unlike AF-2, which binds signature NR boxes in the center of the p160 molecule, AF-1 binds to sequences near the p160 C terminus. We propose that the ability of AF-1 and AF-2 to interact with separate surfaces of the same coactivator is important for the ability of these transactivation functions to synergize. 相似文献
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JN Wilkins A Ashofteh D Setoda WS Wheatley H Huigen W Ling 《Canadian Metallurgical Quarterly》1997,19(1):83-87
Steroid Receptor Coactivator-1 (SRC-1) interacts with nuclear receptors only when they are bound to the ligands and enhance the transactivation. We identified splicing variants encoding three isoforms, SRC-1, SRC-1(-Q), and SRC-1E, generated by alternative usage of an exon(s) and splicing acceptor sites. RT-PCR analysis showed that SRC-1E was more abundantly expressed than SRC-1 or SRC-1(-Q) at the mRNA level in all the cell lines tested. SRC-1E lacks 56 amino acids of SRC-1 and has unique 14 amino acids at the carboxyl terminus, while SRC-1(-Q) differs from SRC-1 by deletion of only one glutamine residue. Since the C-terminal domain of SRC-1 has been shown to be involved in the interaction with nuclear receptors, the enhancement of transactivation by these three isoforms was tested. SRC-1E enhanced thyroid hormone dependent transactivation of reporter gene expression more profoundly than SRC-1 or SRC-1(-Q). Taken together, it was suggested that SRC-1E is the major isoform of SRC-1 to mediate thyroid hormone action. 相似文献
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SA Onate V Boonyaratanakornkit TE Spencer SY Tsai MJ Tsai DP Edwards BW O'Malley 《Canadian Metallurgical Quarterly》1998,273(20):12101-12108
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CA Berrevoets P Doesburg K Steketee J Trapman AO Brinkmann 《Canadian Metallurgical Quarterly》1998,12(8):1172-1183
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A Takeshita PM Yen M Ikeda GR Cardona Y Liu N Koibuchi ER Norwitz WW Chin 《Canadian Metallurgical Quarterly》1998,273(34):21554-21562