病毒性疫苗生产用牛血清质控问题的探讨

<正>目前,绝大部分病毒性疫苗是通过细胞培养制备的。牛血清中含有的多种生物活性物质,如生长因子、激素、脂类、无机盐等成分,是细胞繁殖所需促生长因子和营养物质的来源。牛血清已广泛用于细胞     

用固定化细胞生产L-天门冬氨酸

L-天门冬氨酸在医药、化工和食品工业等方面有多种用途。1.L-天门冬氨酸的镁钾盐用于心脏病、肝脏病、糖尿病的治疗和消除疲劳。此酸的钾盐还用于低钾症的治疗,它的铁盐用于贫血治疗。此外,L-天门冬氨酸和L-鸟氨酸制成的鸟门氨酸注射液,可用于急性和慢性肝炎、肝硬变、肝昏迷、高血氨症的治疗和肝脏手术前后的机能改善。L-天门冬氨酸和多种氨基酸共同     

动态循环提取雷公藤总生物碱的过程研究

雷公藤是一种重要的中药材,其有效成分萜类、生物碱可用于治疗类风湿性关节炎等多种疾病.动态循环提取雷公藤生物碱是雷公藤中药生产中的一个重要工段.今用紫外分光光度法分析总生物碱的含量,测定了雷公藤动态循环提取过程中总生物碱浓度和提取时间的关系.并分析了动态循环提取过程的机理,建立了相应的数学模型,该模型仅含一个参数.模型拟合结果与工业操作数据符合良好,可用于工业动态循环提取过程的模拟和优化.     

可促进慢性创伤愈合的生物医学工程药物研究进展

伤口愈合是一个动态变化的过程,其进程高度依赖于多种信号分子的协调合作。含有生长因子的生物医学工程药物,一直被认为是一种可实现慢性伤口快速和完全愈合的有效方法。可促进慢性伤口愈合的含生长因子类生物工程药物的研发进展迅速,未来的研究重点着眼于这些生长因子的作用机制,以及如何精确控制它们的释药行为,以实现最佳的治疗效果。     

牛乳中抗生素的检测研究

牛乳已广泛运用到我们的生活中,牛乳的研究也成为国内外的研究热点之一。牛乳中营养成分丰富,牛乳中抗生素的检测关系到人们食用的牛乳的安全性。本文主要概述了牛乳的营养成分、牛乳中抗生素的检测办法,希望能够推动牛乳研究的进一步发展,发展出高效率、低成本的抗生素检测方法。     

富自体浓缩生长因子生物学作用的研究进展

富自体浓缩生长因子(concentrated growth factor,CGF)为第三代血小板浓缩产物,是再生医疗领域中诱导组织再生分化的一种新型生物材料。CGF通过差速离心可释放出多种生长因子,如血小板衍生生长因子(plateletderived growth factor,PDGF)、转化生长因子-β(transforming growth factor-β,TGF-β)、类胰岛素生长因子(insulin-like growth factor,IGF)、血管内皮生长因子(vascular endothelial growth factor,VEGF)、表皮生长因子(epidermal growth fcator,EGF)及成纤维细胞生长因子(fibroblast growth factor,FGF)等,这些生长因子相互作用,可发挥促进组织再生的作用。本文就CGF在促进细胞增殖及分化、组织再生及修复、软组织愈合等生物学作用中的研究进展作一综述。     

喹唑啉化合物

该发明涉及了所述化合物的制备方法，用于所述方法的中间体；制备所述中间体的方法；该化合物可用于制备在温血动物体中产生扩血管生成和／或脉管通透性降低作用的药物；这种有价值的化合物可用于治疗多种疾病，包括癌先和类风湿性关节炎。     

碱性成纤维生长因子对皮肤损伤修复作用的研究进展

皮肤损伤是临床常见疾病之一,大多由撕裂、割伤或挫伤等急性创伤导致。在皮肤损伤修复过程中,多种细胞通过分泌细胞因子参与创面愈合,如碱性成纤维生长因子(fibroblast growth factor 2,FGF2或bFGF)、血小板源性生长因子(platelet-derived growth factor,PDGF)和转化生长因子β(transforming growth factor-β,TGF-β)等,其中FGF2可通过促进血管生成、肉芽形成,减少瘢痕形成,加速上皮化促进伤口愈合。FGF2半衰期短,在体内易受酶和皮肤微环境的影响,在伤口处保留时间短暂,降低了其促进伤口愈合的能力,因此,FGF2的稳定性对其临床用于皮肤损伤修复尤为重要。本文就FGF2的分子结构及功能、FGF2受体激活的信号转导途径、FGF2在伤口愈合方面的作用机制及其在整形方面的应用作一综述。     

牛乳中蜡样芽孢杆菌荧光定量PCR检测方法的建立及验证

目的建立一种快速检测牛乳中蜡样芽孢杆菌的荧光定量PCR方法,并进行验证。方法根据GenBank中登录的蜡样芽孢杆菌gyrB基因序列及荧光定量PCR要求,设计合适的特异性引物,提取菌体DNA,对目的基因进行扩增及克隆;以重组质粒为模板进行荧光定量PCR扩增,绘制标准曲线;以蜡样芽孢杆菌DNA为模板进行荧光定量PCR扩增,确定其扩增条件。对建立的方法进行特异性及灵敏性验证。结果建立的方法检测其他3种牛乳中常见致病菌(大肠埃希菌、志贺菌、沙门菌)未见特异性扩增,即无交叉反应;最低检出限为1×10~(-7) ng/μL。以掺入蜡样芽孢杆菌的牛乳为样品,可检测到牛乳中10. 4 CFU/mL的蜡样芽孢杆菌,优于国标方法(GB 4789. 14-2014)检测含有相同菌落数的蜡样芽孢杆菌(仅能检测到1. 04×10~2 CFU/mL)。结论建立的方法特异性强,灵敏性高,适用于快速、准确检测牛乳中的蜡样芽孢杆菌,为实验室快速检测致病菌提供了参考。     

动态

双乙烯酮双乙烯酮是一种有机合成的重要原料,可用于生产多种精细化工中间体,如合成丁酮酸、乙酸醋酯类、吡唑酮类等,也可广泛地用于制造增塑剂、染料、合成纤维等,其中医药行业中用于生产氨基比林、安乃近等解热镇痛药物,其消耗量约占双乙烯酮年总产量的50%左右.此外,农药行业用于生产杀虫剂等也需40%的双乙烯酮,其余用于染料、饲料等行业.     

Insect antifeedant properties of anthranoids from the genusVismia

Vismiones and ferruginins, representatives of a new class of lypophilic anthranoids from the genusVismia were found to inhibit feeding in larvae of species ofSpodoptera, Heliothis, and inLocusta migratoria.     

Direct observation of freeze-thaw instability of latex coatings via high pressure freezing and cryogenic SEM

Despite its industrial importance, the subject of freeze-thaw (F/T) stability of latex coatings has not been studied extensively. There is also a lack of fundamental understanding about the process and the mechanisms through which a coating becomes destabilized. High pressure (2100 bar) freezing fixes the state of water-suspended particles of polymer binder and inorganic pigments without the growth of ice crystals during freezing that produce artifacts in direct imaging scanning electron microscopy (SEM) of fracture surfaces of frozen coatings. We show that by incorporating copolymerizable functional monomers, it is possible to achieve F/T stability in polymer latexes and in low-VOC paints, as judged by the microstructures revealed by the cryogenic SEM technique. Particle coalescence as well as pigment segregation in F/T unstable systems are visualized. In order to achieve F/T stability in paints, latex particles must not flocculate and should provide protection to inorganic pigment and extender particles. Because of the unique capabilities of the cryogenic SEM, we are able to separate the effects of freezing and thawing, and study the influence of the rate of freezing and thawing on F/T stability. Destabilization can be caused by either freezing or thawing. A slow freezing process is more detrimental to F/T stability than a fast freezing process; the latter actually preserves suspension stability during freezing. Presented at the 82nd Annual Meeting of the Federation of Societies for Coatings Technology, October 27–29, 2004 in Chicago, IL. Tied for first place in The John A. Gordon Best Paper Competition.     

Ethanol and (−)-α-Pinene: Attractant Kairomones for Bark and Ambrosia Beetles in the Southeastern US

In 2002–2004, we examined the flight responses of 49 species of native and exotic bark and ambrosia beetles (Coleoptera: Scolytidae and Platypodidae) to traps baited with ethanol and/or (−)-α-pinene in the southeastern US. Eight field trials were conducted in mature pine stands in Alabama, Florida, Georgia, North Carolina, and South Carolina. Funnel traps baited with ethanol lures (release rate, about 0.6 g/day at 25–28°C) were attractive to ten species of ambrosia beetles (Ambrosiodmus tachygraphus, Anisandrus sayi, Dryoxylon onoharaensum, Monarthrum mali, Xyleborinus saxesenii, Xyleborus affinis, Xyleborus ferrugineus, Xylosandrus compactus, Xylosandrus crassiusculus, and Xylosandrus germanus) and two species of bark beetles (Cryptocarenus heveae and Hypothenemus sp.). Traps baited with (−)-α-pinene lures (release rate, 2–6 g/day at 25–28°C) were attractive to five bark beetle species (Dendroctonus terebrans, Hylastes porculus, Hylastes salebrosus, Hylastes tenuis, and Ips grandicollis) and one platypodid ambrosia beetle species (Myoplatypus flavicornis). Ethanol enhanced responses of some species (Xyleborus pubescens, H. porculus, H. salebrosus, H. tenuis, and Pityophthorus cariniceps) to traps baited with (−)-α-pinene in some locations. (−)-α-Pinene interrupted the response of some ambrosia beetle species to traps baited with ethanol, but only the response of D. onoharaensum was interrupted consistently at most locations. Of 23 species of ambrosia beetles captured in our field trials, nine were exotic and accounted for 70–97% of total catches of ambrosia beetles. Our results provide support for the continued use of separate traps baited with ethanol alone and ethanol with (−)-α-pinene to detect and monitor common bark and ambrosia beetles from the southeastern region of the US.     

Methyl 2,4,6-decatrienoates Attract Stink Bugs and Tachinid Parasitoids

Halyomorpha halys (Stål) (Pentatomidae), called the brown marmorated stink bug (BMSB), is a newly invasive species in the eastern USA that is rapidly spreading from the original point of establishment in Allentown, PA. In its native range, the BMSB is reportedly attracted to methyl (E,E,Z)-2,4,6-decatrienoate, the male-produced pheromone of another pentatomid common in eastern Asia, Plautia stali Scott. In North America, Thyanta spp. are the only pentatomids known to produce methyl 2,4,6-decatrienoate [the (E,Z,Z)-isomer] as part of their pheromones. Methyl 2,4,6-decatrienoates were field-tested in Maryland to monitor the spread of the BMSB and to explore the possibility that Thyanta spp. are an alternate host for parasitic tachinid flies that use stink bug pheromones as host-finding kairomones. Here we report the first captures of adult and nymph BMSBs in traps baited with methyl (E,E,Z)-2,4,6-decatrienoate in central Maryland and present data verifying that the tachinid, Euclytia flava (Townsend), exploits methyl (E,Z,Z)-2,4,6-decatrienoate as a kairomone. We also report the unexpected finding that various isomers of methyl 2,4,6-decatrienoate attract Acrosternum hilare (Say), although this bug apparently does not produce methyl decatrienoates. Other stink bugs and tachinids native to North America were also attracted to methyl 2,4,6-decatrienoates. These data indicate there are Heteroptera in North America in addition to Thyanta spp. that probably use methyl 2,4,6-decatrienoates as pheromones. The evidence that some pentatomids exploit the pheromones of other true bugs as kairomones to find food or to congregate as a passive defense against tachinid parasitism is discussed.     

2007～2008年世界塑料工业进展

收集了2007年7月～2008年6月世界塑料工业的相关资料,介绍了2007～2008年国外塑料工业的发展情况,提供了世界塑料产量、消费量及全球各类树脂的需求量及产能情况.按通用热塑性树脂(聚乙烯、聚丙烯、聚苯乙烯、聚氯乙烯、ABS树脂)、工程塑料(尼龙、聚碳酸酯、聚甲醛、热塑性聚酯、聚苯醚)、特种工程塑料(聚苯·硫醚、液晶聚合物、聚醚醚酮)、通用热固性树脂(酚醛、聚氨酯、不饱和聚酯树脂、环氧树脂)不同品种的顺序,对树脂的产量、消费量、供需状况及合成工艺、产品应用开发、树脂品种的延伸及应用的进一步扩展等技术作了详细介绍.     

2005～2006年国外塑料工业进展

收集了2005年7月～2006年6月国外塑料工业的相关资料,介绍了2005—2006年国外塑料工业的发展情况。提供了世界塑料产量、消费量及全球各类树脂生产量以及各国塑料制品的进出口情况。作为对比,介绍了中国塑料的生产情况。按通用热塑性树脂（聚乙烯、聚丙烯、聚苯乙烯、聚氯乙烯、ABS树脂）、工程塑料（聚酰胺、聚碳酸酯、聚甲醛、热塑性聚酯、聚苯醚）、通用热固性树脂（酚醛、聚氨酯、不饱和树脂、环氧树脂）、特种工程塑料（聚苯硫醚、液晶聚合物、聚醚醚酮）的品种顺序,对树脂的产量、消费量、供需状况及合成工艺、产品开发、树脂品种的延伸及应用的扩展作了详细的介绍。