静脉输注免疫球蛋白治疗特发性血小板减少性紫癜

大剂量静脉输注免疫球蛋白（IVIg）治疗特发性血小板减少性紫癜（ITP）20例，15天之内100％病例血小板升至5×1010／L以上．70％病例血小板升至1×1011／L以上，与激素治疗组相比，具有血小板升速快，幅度大的优点。经激素治疗无效的患者，用大剂量IVIg治疗亦有效，使用中未发现毒副作用。     

石棉与吸附工艺对电槽运行的影响

１石棉１１石棉的地质情况石棉是一种纤维状含水硅酸镁的非金属矿物,其分子式为３ＭｇＯ·２ＳｉＯ２·２Ｈ２Ｏ,理论含量ｗ（ＭｇＯ）４３．４６％、ｗ（ＳｉＯ２）４３．５％、ｗ（Ｈ２Ｏ）１３．０４％。可开采石棉主要取决于地质条件的变化,根据对现有化工用石棉...     

苏·阿防治花椰菜小菜蛾的效果

比较了苏·阿、Ｂ．ｔ．和虫螨克防治花椰菜小菜蛾的效果。药后１天,２％苏·阿可湿性粉 剂１００ｇ／６６７ｍ２的防效为９８．３％,高于Ｂ．ｔ．可湿性粉剂（１６０００ＩＵ／ｍｇ）５０ｇ／６６７ｍ２９５．９％的防效, 也高于０．９ ％虫螨克乳油 ５０ｍｌ／６６７ｍ２９５．４ ％的防效。药后１周,苏·阿的防效仍达 ９４．７７％,明显 高于Ｂ．ｔ．可湿性粉剂（１６０００ＩＵ／ｍｇ）５０ｇ／６６７ｍ２和０．９％虫螨克乳油５０ｍｌ／６６７ｍ２的防效。     

厌氧流化床处理硫酸盐草浆废水的研究

本文研究了ＡＦＢ反应器在中温（３０±２）℃条件下处理硫酸盐草浆废水的性能，其达到的指标为：当进水ＣＯＤ浓度２０００～５０００ｍｇ／Ｌ，水力停留时间３～９ｈ，ＣＯＤ去除率为５０．１％～７０．２％，容积产气率１．４６～２．０ｍ３／ｍ３·ｄ，有机容积负荷达４３．２ｋｇＣＯＤ／ｍ３·ｄ；并初步研究了废水中ＳＯ２－４、Ｓ２－等物质对厌氧生物的抑制作用，提出了消除其影响的方法。     

间磺酸基偶氮氯膦褪色光度法测定微量铬（Ⅵ）

研究了间磺酸基偶氮氯膦与Ｃｒ（ＶＩ）褪色反应最佳条件。在１．０ｍｏｌ／ＬＨ２ＳＯ４介质中。褪色反应的灵敏度ε＝３．１×１０￣４Ｌ／ｍｏｌ·ｃｍ，Ｃｒ（ＶＩ）含量在０．１２～１．２μｇ／ｍｌ范围内遵从比尔定律。     

新发光试剂N-(β-羧基丙酰基)异鲁米诺的合成及性能的研究

合成了新发光试剂Ｎ－（β－羧基丙酰基）异鲁米诺（ＣＰＩＬ），并对其化学发光性能进行了研究。ＣＰＩＬ在ＮａＨＣＯ３－ＮａＯＨ介质中与Ｈ２Ｏ２反应，线性范围为２×１０－１０～１×１０－８ｍｏｌ／ＬＣＰＩＬ，检测限为１×１０－１０ｍｏｌ／Ｌ，测定１×１０－９ｍｏｌ／Ｌ　ＣＰＩＬ溶液，相对标准偏差为６．４％。Ｃｏ２＋对ＣＰＩＬ的化学发光反应具有催化增强作用（增强１０倍），当体系中加入一定量的Ｃｏ２＋溶液时，ＣＰＩＬ的浓度在４×１０－１１～１×１０－９ｍｏｌ／Ｌ从范围内具有良好的线性关系，检测限为８×１０－１２ｍｏｌ／Ｌ，灵敏度高于文献报道的ＡＢＥＩ－Ｃｏ２－ＮａＣＩＯ发光体系。     

氮源对里氏木酶合成木聚糖酶的影响

分别以（ＮＨ４）２ＳＯ４、酵母浸膏及蛋白胨为产酶氮源制备木聚糖酶。试验结果表明,酵母浸膏的产酶效果最好,其次是（ＮＨ４）２ＳＯ４。当以酵母浸膏为氮源产酶时,酶活力最高达到２５．４７ＩＵ／ｍＬ,酶得率和酶产率分别为３６３８．６ＩＵ／ｇ木聚糖和８４９０．０ＩＵ／Ｌ·ｄ,酶活力分别是以（ＮＨ４）２ＳＯ４及蛋白胨为氮源时的１．５倍和２．０倍     

管形电收尘器算图数则

管形电收尘器算图数则章开慈（福州硫酸厂３５００１４）１电晕电流算图１计算公式Ｉ＝２ＫＶ（Ｖ－Ｖ０）９Ｒ２ｌｎＲｒ０（１）Ｉ——电晕电流，ｍａ／ｍ；Ｋ——气体离子迁移率，ｃｍ２／ｖ·ｓ；Ｖ——运行电压，ｋＶ；Ｖ０——起晕电压，ｋＶ；Ｒ——收尘极半径，ｃ...     

高效液相色谱法测定快·扑可湿性粉剂中二氯喹啉酸和扑草净

采用高效液相色谱法测定快·扑可湿性粉剂中的二氯喹啉酸（快杀稗）和扑草净。建立了二氯喹啉酸和扑草净的高效液相色谱测定方法,色谱条件为以ＹＷＧ－Ｃ１８国固定相,甲醇－水（８０：２０）为流动相,紫外检测波长２５４ｎｍ,流速为１ｍｌ／ｍｉｎ。二氯喹啉酸和扑草净的变异系数为１．４７８０％和２．８１６１％,平均回收率分别为９７．０７％和９８．１７％,工作曲线的相关系数分别为：ｒ二＝０．９８７４、ｒ扑＝０．９７     

苯乙烯- 丙烯酸丁酯- 丙烯酸羟乙酯共聚物的合成及其与二异氰酸酯的交联

当混合溶剂乙酸丁酯／二甲苯质量比为１∶１,过氧化苯甲酰（ＢＰＯ）为１％,苯乙烯（Ｓｔ）／丙烯酸丁酯（ＢＡ）＋丙烯酸羟乙酯（ＨＥＡ）（质量比）为６２∶３８,反应温度为１３３℃时,可获得转化率为９５．３％、［η］为０．１６ｄＬ／ｇ的Ｓｔ－ＢＡ－ＨＥＡ共聚物。该共聚物（ＨＥＡ的结合量为２％～４％）在同一溶剂中加入甲苯二异氰酸酯（ＴＤＩ）或聚醚型二异氰酸酯（ＰＥＤＩ）,当—ＮＣＯ／—ＯＨ摩尔比为１．２～１．５∶１,二丁基二月桂酸锡用量为０．３％～１．０％时,可于室温下交联,交联度为５８．３％～５９．１％     

The Role of Leptin in Childhood Immune Thrombocytopenia (ITP): An Anti-Inflammatory Agent?

To investigate the effect of leptin in childhood ITP, we measured plasma leptin in 39 children with acute ITP, after treatment and in remission, and in 33 healthy age/BMI-matched controls. We also cultured ITP and control peripheral blood mononuclear cells (PBMCs) with recombinant leptin to assess its direct effect on pro/anti-inflammatory cytokine gene expression. A significant increase in leptin was observed in children with active disease compared to controls. A significant inverse correlation of leptin with platelet count was also observed in children with acute ITP. Leptin remained high after treatment with IVIg, whereas steroid treatment lowered leptin below control levels. In remission, leptin was in the control range. Cytokine gene expression was significantly increased in children with acute ITP compared with controls, with highest expression for IFN-γ and IL-10. IVIg/steroid treatment significantly decreased IFN-γ and IL-10 expression. In remission, IFN-γ and IL-10 expression remained low. Addition of leptin to PBMCs isolated from patients in remission resulted in a significant increase in IL-10 gene expression compared to controls. Further experiments with purified T-cells and monocytes identified monocytes as the source of leptin-induced IL-10. We suggest that leptin acts as an active anti-inflammatory agent in childhood ITP by promoting IL-10 secretion by monocytes.     

MTX-抗体偶联物对单核-巨噬细胞的封闭和杀伤作用

目的　观察静脉注射用免疫球蛋白与免疫抑制剂偶联物对单核—巨噬细胞的杀伤作用 ,为其临床治疗ITP奠定实验基础。方法　分别用间接和直接交联法将IVIG与MTX制备成偶联物 ,用间接免疫荧光法检测偶联物Fc段结合活性 ,以Fc受体阳性的小鼠巨噬细胞和人单核细胞白血病细胞株U937为靶细胞 ,用MTT法测定偶联物的杀伤活性。结果　偶联物对巨噬细胞的杀伤作用明显大于游离MTX ;偶联物对Fc受体阳性细胞的杀伤作用明显大于Fc受体阴性细胞。间接偶联物IVIG HSA MTX杀伤作用明显高于直接偶联物IVIG MTX。结论　MTX抗体偶联物在体外对单核—巨噬细胞显示了相对特异的杀伤活性     

自体血小板胶中转化生长因子-β1浓度的影响因素

目的探讨自体血小板胶(Autologous platelet-rich gel,APG)中转化生长因子-β1(Transforming growth factor beta1,TGF-β1)浓度的影响因素。方法用一体性塑料血袋分别采集40名健康人的静脉血50 ml,采用两次离心法制备富血小板血浆(Platelet-rich plasma,PRP),用血细胞分析仪检测静脉血和PRP中的血小板数目;将PRP与凝血酶和钙剂混合液按体积比10∶1的比例混合,制备APG,采用ELISA法定量分析APG中的TGF-β1浓度;选择5个参数(年龄、性别、静脉血血小板计数、PRP中血小板平均计数及静脉血制备前保存温度)进行多元线性回归分析。结果 PRP中血小板平均计数[(1 232.23±195.45)×109个/L]约为静脉血[(195.95±42.29)×109个/L]的6倍;APG中TGF-β1的平均浓度为(5.83±2.31)ng/ml,与制备前静脉血保存温度密切相关(P<0.000 5),而与年龄、性别、静脉血血小板计数和PRP中血小板计数无关。结论制备前APG的静脉血样本应保存于室温(20～24℃)下。     

pRL-hTNF/JM103工程菌发酵和rhTNFα表达的研究

为便于下游中试规模生产,采用温度敏感型启动子PRPL构建rhTNFα工程菌,并对影响工程菌发酵培养和表达的因素进行了初步研究。结果显示,采用RTB培养基,50℃热水快速升温,诱导培养4～4．5h,湿菌体收获率和表达量均达到较高水平;50L发酵罐连续发酵3批,14000r／min连续离心,菌体收获率湿重达16．gg／L培养物,rhTNFα表达量占菌体总蛋白的10.5％,活性达1．35×107U／mg蛋白。.     

提取方法对4种中药雌激素活性的影响

针对4种具有雌激素活性的中药———虎杖、葛根、蛇麻花和女贞子,分别采用水煎和w(C2H5OH)=70%的乙醇水溶液超声法进行提取,用转基因酵母法对其提取产物进行了雌激素活性的测试,对比研究了两种提取方法对4种中药雌激素活性的影响。实验结果显示,虎杖水煎和超声提取样品的EC50分别为3.5×10-3和1×10-3g/L;葛根水煎和超声提取样品的EC50分别为3.5×10-2和1×10-2g/L;女贞子水煎提取样品不显示活性,而超声法提取样品的EC50为2.5×10-3g/L;蛇麻花用这两种方法提取的样品均检测不出雌激素活性。     

合成香料中间体废水预处理技术研究

合成香料中间体废水(COD为132580 mg/L)采用电渗析→气浮除油→电-Fenton的处理途径。结果表明,在I=1.5 A,电解时间为120 min的条件下,电渗析效果最佳,R=6.7322×10-6mol/(mL.cm2);电渗析后调节pH值为3时气浮除油,COD去除率可达到59.8%,电-Fenton的最佳反应条件为:pH为3.5,I=0.75 A,Fe2+投加量为3 g/L,H2O2与Fe2+的摩尔比为10∶1,反应时间为75 min,此条件下废水COD去除率可达66.62%。3步处理后,总COD去除率达86.60%。     

活性炭生物转盘的制备及处理有机模拟废水

为了寻找一种质轻高效的新型生物转盘盘片材料,实验以活性炭作为生物膜附着的载体,聚丙烯塑料板为基体,采用化学氧化-铁离子覆盖技术制备了活性炭生物转盘盘片。使用该活性炭生物转盘处理有机模拟废水,以COD和NH3—N质量浓度变化为评价指标,借助微生物显微镜观察了挂膜期间微生物的生长过程。结果表明,该盘片具有独特的微生物吸附能力,微生物量达到2.60×109个/g,生物膜干质量为16.909 6 g/m2;在进水初始COD质量浓度为1 000 mg/L、NH3—N质量浓度为100 mg/L左右时,当HRT为12 h时,盘片的有机去除负荷为5.61 g/m2;当循环进水3 d时,COD和NH3—N去除率均在80%以上,该三级生物转盘反应器各级污染物去除率均较高,第1级去除效果最显著,处理后水质达到了国家污水综合排放标准(GB8978-96)的二级标准。     

Elucidating the Mechanism of Action of the Attributed Immunomodulatory Role of Eltrombopag in Primary Immune Thrombocytopenia: An In Silico Approach

Eltrombopag is a thrombopoietin receptor (MPL) agonist approved for the treatment of primary immune thrombocytopenia (ITP). Recent evidence shows that some patients may sustain platelet counts following eltrombopag discontinuation. The systemic immunomodulatory response that resolves ITP in some patients could result from an increase in platelet mass, caused either by the direct action of eltrombopag on megakaryocytes through MPL stimulation, or potential MPL-independent actions on other cell types. To uncover the possible mechanisms of action of eltrombopag, in silico analyses were performed, including a systems biology-based approach, a therapeutic performance mapping system, and structural analyses. Through manual curation of the available bibliography, 56 key proteins were identified and integrated into the ITP interactome analysis. Mathematical models (94.92% mean accuracy) were obtained to elucidate potential MPL-dependent pathways in non-megakaryocytic cell subtypes. In addition to the effects on megakaryocytes and platelet numbers, the results were consistent with MPL-mediated effects on other cells, which could involve interferon-gamma, transforming growth factor-beta, peroxisome proliferator-activated receptor-gamma, and forkhead box protein P3 pathways. Structural analyses indicated that effects on three apoptosis-related proteins (BCL2L1, BCL2, BAX) from the Bcl-2 family may be off-target effects of eltrombopag. In conclusion, this study proposes new hypotheses regarding the immunomodulatory functions of eltrombopag in patients with ITP.     

分泌型重组人粒细胞-巨噬细胞集落刺激因子中试发酵工艺的研究

通过对GM－CSF工程菌W3100发酵条件的研究，优化了培养及表达条件，使工程菌W3100在30L发酵罐可表达分泌型GM－CSF1.5g／L左右，占菌体总蛋白的25％以上，纯化后的比活大于2．0×107。