Characteristics of functional brown rice prepared by parboiling and microwave drying

Brown rice (BR) is well known for its functional properties; however, it is considered unsuitable for consumption in modern diet owing to its hard texture, low digestibility, and high lipid peroxidation. In this study, we investigated the physicochemical properties, and cooking and storage characteristics of functional BR prepared by parboiling (100°C, 15 min) and microwave drying (100 kW, 8 rpm, 91°C, 40 min) processes. These processes significantly increased resistant starch (RS) content by 1.5-fold and total dietary fiber (TDF) content by 1.8-fold; Parboiling and microwave drying processes increased the umami taste by 10% using electronic tongue and decreased the hardness by 10% using texture analyzer, respectively. Additionally, irrespective of the storage duration, these processes remarkably decreased acidity and bacterial counts in the parboiled BR group compared to that in the BR group. Presumably, higher RS and TDF contents, enhanced umami taste, and longer shelf-life of BR would help patients with obesity and diabetes, and industries developing relevant high-value food products.     

Effect of debranching and heat treatments on formation and functional properties of resistant starch from high-amylose corn starches

High-amylose corn starches [(Hylon V (H5) and Hylon VII (H7)] were debranched with pullulanase, followed by autoclaving–storing cycles and drying in an oven (at 50 °C) or freeze-dryer. The samples were autoclaved at 123 and 133 °C and stored at 4 and 95 °C. Molecular weights of the samples decreased and resistant starch (RS) contents increased with increased debranching time. RS contents of H7 samples were higher than those of H5 samples. RS contents of oven-dried samples were higher than those of freeze-dried samples. Debranching caused decreases in DSC peak temperature (T _p) and increases in enthalpy (ΔH) values of H5 and H7. Autoclaving at 133 °C caused higher ΔH values as compared to autoclaving at 123 °C. The solubility and water-binding values of autoclaved-only (control) and autoclaved–debranched (3–48 h) samples and the samples treated with autoclaving–storing cycles after debranching of both H5 and H7 were higher than those of their respective native starches. Debranching of starch samples affected the emulsion capacity of albumin adversely, but improved the emulsion stability of albumin. Cold viscosity values of freeze-dried samples were higher than those of oven-dried samples. Autoclaving–storing cycles after debranching caused decreases in peak, breakdown and final viscosity values.     

Effect of single and dual hydrothermal treatments on the crystalline structure,thermal properties,and nutritional fractions of pea,lentil, and navy bean starches

Pea, lentil and navy bean starches were annealed at 50 °C (70% moisture) for 24 h and heat-moisture treated at 120 °C (30% moisture) for 24 h. These starches were also modified by a combination of annealing (ANN) and heat-moisture treatment (HMT). The impact of single and dual modifications (ANN–HMT and HMT–ANN) on the crystalline structure, thermal properties, and the amounts of rapidly digestible starch (RDS), slowly digestible starch (SDS), and resistant starch (RS) were investigated. Birefringence remained unchanged on ANN but decreased on HMT. Granular swelling and amylose leaching decreased on ANN and HMT. Relative crystallinity, gelatinization enthalpy, and short-range order on the granule surface increased on ANN but decreased on HMT. Gelatinization transition temperatures increased on ANN and HMT. Gelatinization temperature range decreased and increased on ANN and HMT, respectively. ANN and HMT increased SDS and decreased RS levels in all starches. However, RDS levels increased on ANN and HMT in pea and lentil starches but decreased in navy bean starch. In gelatinized starches, ANN and HMT decreased RDS level and increased SDS and RS levels. Changes to crystalline structure, thermal properties and amounts of RDS, SDS, and RS were modified further on ANN–HMT and HMT–ANN.     

Formation of Resistant Starch in Corn Starch and Estimation of its Content from Physicochemical Properties

The effect of autoclaving temperatures (100‐120°C) on yields of enzyme‐resistant starch (RS) from normal corn starch and the physicochemical properties of autoclaved‐cooled starches were studied. The RS content increased linearly with increasing autoclaving temperature (R² = 0.993) and the number of autoclaving‐cooling cycles at an autoclaving temperature of 120°C. The effect of the number of autoclaving‐cooling cycles was more pronounced than that of temperature. The swollen starch weight measured at 60°C slightly increased as the RS content increased, and then drastically decreased with the continous increase of the RS content (R² = ‐0.969). As the RS content increased, all parameters of Rapid Visco Analyser (RVA) viscosity except breakdown decreased. Log RVA peak viscosity showed a negative correlation with the RS content (R² = ‐0.986). The enthalpy in the differential scanning calorimetry (DSC) endotherm corresponding to the transitions of RS linearly increased as the RS content increased (R² = 0.988). The RS content of the heat‐treated starch estimated from the relationship between RS content and swollen starch weight at 60°C, log RVA peak viscosity or DSC enthalpy was in good agreement with that determined with the standard method.     

Effect of gelatinisation on slowly digestible starch and resistant starch of heat-moisture treated and chemically modified canna starches

The effects of gelatinisation on slowly digestible (SDS) and resistant starch (RS) of native and modified canna starches were investigated. Starch slurries (10% w/w) were gelatinised at 100 °C for 5, 10, 20 and 40 min using a rapid visco analyzer (RVA). Significant change in the degree of gelatinisation (DG) values of all starch samples was observed during the initial 10 min of gelatinisation; after that the DG values increased gradually with gelatinisation time. The RS contents in all gelatinised starches decreased with increasing gelatinisation time, while the SDS values fluctuated. Chemical modification affected DG values as well as RS/SDS contents. The RS contents in 10% (w/w) acetylated, hydroxypropylated, octenyl succinylated and cross-linked canna starches gelatinised at 100 °C for 40 min were 26.6%, 32.0%, 45.3% and 19.8%, respectively, which were higher than that of the native starch (12.4%). Canna starch modified by crosslinking had the highest SDS content when gelatinised for 20-40 min. Modification of canna starch by heat-moisture treatment resulted in a lower content of RS for all treated samples. However, the Vt-HMT25 (canna starch containing moisture content of 25% during heat treatment) when gelatinised for 5-20 min contained a higher amount of SDS, compared to unmodified starch. The most effective modification method for RS and SDS formation was octenyl succinylation, where the sum of RS and SDS approached that of Novelose260.     

Effects of Gelatinization and Gel Storage Conditions on the Formation of Canna Resistant Starch

The effects of gelatinization and gel storage conditions on the formation of canna resistant starch (RS) were investigated. Starch slurries (10%, dwb) were autoclaved at 121?°C for 30, 60, and 120?min. The gels obtained were subsequently stored at different temperatures (4?°C, 30?°C, and 100?°C) and times (0, 1, 3, 5, and 7?days). Analyses of the RS content in gelatinized starch samples in comparison with that in granular starch showed that the RS fraction in granular starch was very high (97.3% w/w); however, nearly all of the RS was thermally unstable, as indicated by a great reduction in RS content (to 1.9% w/w) after cooking at 100?°C for 20?min. The RS contents in gelatinized starch samples were 12.0?C15.9% w/w, which were reduced to 7.9?C10.8% w/w after cooking. Storage of gels resulted in a significant increase in the amount of the thermally stable RS fraction, e.g., a thermally stable RS content of 16.8% w/w was found in the gel sample gelatinized for 120?min and stored at 4?°C for 3?days. This indicated that the ordered structures of the RS portion were tightened under the storage conditions. The gelatinization temperature of canna starch was 72.2?°C, whereas the RS products exhibited two melting temperature ranges, 51.1?C76.3?°C and 163.1?C165.1?°C, indicating that the newly formed crystals were very strong.     

Effect of hydrothermal treatment of runner bean (Phaseolus coccineus) seeds and starch isolation on starch digestibility

The study investigated the effect of traditional soaking and cooking, storage after cooking and freezing (? 18 °C, 21 days) and autoclaving of two varieties of runner bean on starch digestibility. Results achieved were compared with digestibility of isolated starch subjected to similar treatments. The digestibility of native starch from Nata var. seeds was lower after isolation than in raw flour. This starch was characterized by a higher content of fat and lower values of swelling power (SP) and amylose leaching (AML). After the thermal treatment, a significantly higher content of rapidly digestible starch (RDS) was observed both in seeds and starch. It was accompanied by reduced contents of resistant starch (RS) and slowly digestible starch (SDS). In flours from cooked seeds, the content of RDS was observed to be higher than in flours from autoclaved seeds, despite similar changes in contents of other constituents (ash and protein). It was probably due to better starch gelatinization owing to the long-lasting soaking of seeds. This resulted in a greater decrease of amylose content of starch compared to the other flours. Differences in SP, AML and thermal properties between starches isolated from two bean varieties had no influence on their digestibility after cooking. The storage of starch pastes at a temperature of ? 18 °C, unlike that of seeds, resulted in a significant increase in RS content, which shows the importance of other flour components in the process of starch retrogradation.     

Retrogradation of partially gelatinised potato starch prepared by ball milling

This study was performed to evaluate the effect of partial gelatinisation on the retrogradation of modified potato starch. The partially gelatinised starches with gelatinisation degree at mean levels of 22.47%, 49.18%, 76.80% and 86.19% were prepared by ball milling (0.5, 1.5, 3 and 10 h). The thermal properties and crystal structure of retrograded starch were examined during 21 days of refrigerated storage at 4 °C. Retrograded starch with high initial gelatinisation degree (86.19%) showed higher retrogradation enthalpy of 6.12 ± 0.18 J g^?1 and lower onset temperature of 45.41 ± 0.24 °C than sample with low gelatinisation degree (22.47%) where the results were 1.32 ± 0.18 J g^?1 and 54.05 ± 0.03 °C, respectively. During storage, two peaks in the X‐ray diffractograms for starch with high gelatinisation degree appeared and increased rapidly, while the peaks for starch with low gelatinisation degree increased slowly. These results suggest that a certain amount of remainder crystals presented in partially gelatinised starch impeded the retrogradation.     

Structural characterizations and digestibility of debranched high-amylose maize starch complexed with lauric acid

Structural characterizations and digestibility of debranched high-amylose maize starch complexed with lauric acid (LA) were studied. The cooked starch was debranched by using pullulanase and then complexed. Light microscopy showed that the lipids complexed starches had irregularly-shaped particles with strong birefringence. Gel-permeation chromatograms revealed that amylopectin degraded to smaller molecules during increasing debranching time, and the debranch reaction was completed at 12 h. Debranching pretreatment and prolonged debranching time (from 2 h to 24 h) could improve the formation of starch lipids complex. X-ray diffraction pattern of the amylose–lipid complexes changed from V-type to a mixture of B- and V-type polymorphs and relative crystallinity increased as the debranching time increased from 0 to 24 h. In DSC thermograms, complexes from debranched starch displayed three separated endotherms: the melting of the free lauric acid, starch–lipid complexes and retrograded amylose, respectively. The melting temperature and enthalpy changes of starch–lipid complex were gradually enhanced with the increasing of debranching time. However, no significant enthalpy changes were observed from retrograded amylose during the starch–lipid complex formation. Rapidly digestible starch (RDS) content decreased and resistant starch (RS) content increased with the increasing of debranching time, while the highest slowly digestible starch (SDS) content was founded at less debranching time of 2 h. The crystalline structures with dense aggregation of helices from amylose-LA complex and retrograded amylose could be RS, while SDS mostly consisted of imperfect packing of helices between amylopectin residue and amylose or LA.     

Effect of processing on starch fractions in different varieties of finger millet

The effect of processing on starch fractions in finger millet (ragi) was studied in a standardized system and convenience mixes. In raw varieties, rapidly available starch (RDS) was 8.3% to 11.1% with slowly digestible starch (SDS) of 26.4% to 35.3%, and total available starch (TAS) 39–53%. The changes in the SDS and TAS were statistically significant. Puffing resulted in an increased RDS and decreased SDS. The changes were more prominent in hill grown varieties as compared to base varieties. Resistant starch (RS) ranged from 0.9% to 1.0% among raw varieties and decreased during puffing. The effect of puffing on gelatinisation of ragi flour was investigated, MR-1 variety showed a single phase transition with gelatinisation temperature of 64 °C, the same was found to be absent in puffed flour reflecting the gelatinisation during puffing. RS formation was influenced by the processing methods, increased during pressure cooking and roasting while dietary fiber increased during cooking and both decreased in all other processing methods. However, its implication in the convenience mix has shown that other ingredients also play a role in the RS content.     

In vitro starch digestibility of fresh and sun‐dried faba beans (Vicia faba L.)

Fresh and sun‐dried faba beans (Vicia faba L.) were cooked, stored for various times at 4 °C and analysed for available starch (AS), resistant starch (RS) and fibre‐associated resistant starch (FARS) contents as well as α‐amylolysis. Fresh beans required a shorter cooking time (25 min) than dried beans (158 min). Cooked fresh faba beans had a higher AS content than cooked dried faba beans. The AS content in both decreased during cold storage, with fresh beans showing a smaller decrease than dried beans with increasing storage time. Cooked fresh faba beans also had a higher total RS content than cooked dried faba beans, although a greater increase in RS content was recorded in the latter upon storage. Starch retrogradation was more prominent in cooked dried faba beans than in cooked fresh faba beans, as indicated by the consistently higher FARS content. The α‐amylolysis rate decreased with increasing storage time, i.e. long‐stored (72 h) cooked faba beans exhibited slower starch digestion, and differences were recorded between fresh and dried beans. The predicted glycaemic index ranged between 60.9 and 58.0% for cooked fresh faba beans and between 57.9 and 55.8% for cooked dried faba beans, which is suggestive of slow glucose release from starch in faba beans. Copyright © 2007 Society of Chemical Industry     

Effect of debranching and storage condition on crystallinity and functional properties of cassava and potato starches

Debranching starch by pullulanase is considered to improve the RS content of starch which is widely used to produce the starch‐based foods with high‐health benefit impacts. In this study, the cassava and potato starches were debranched by pullulanase, followed by an autoclave treatment and storage at −18°C, 4°C, or 25°C to investigate their crystallinity and functional properties. After debranching, the potato starch contained significantly higher CL (35.4 glucose units) than did the cassava starch (32.4 glucose units). The debranched cassava and potato starches after retrogradation at the storage temperatures had a typical B‐type crystalline structure although the native cassava and potato starches exhibited the different crystalline forms (A‐ and B‐type, respectively). The RS contents of the debranched cassava and potato starches significantly improved with higher RS content of the debranched potato starch than that of the debranched cassava starch at the same storage condition. The storage temperature significantly affected the RS formation of the debranched starches with the highest RS content at storage temperature of −18°C (35 and 48% for the debranched cassava and potato starches, respectively). The debranched starches had significantly lower viscosities and paste clarities but higher solubilities than did the native starches. As a result, the debranched cassava and potato starches can be considered for use not only in functional foods with enhanced health benefits but also in pharmaceutical and cosmetic industries.     

Effect of tea polyphenols on the retrogradation of rice starch

The effect of tea polyphenols (TPLs) on the retrogradation of rice starch (RS) was investigated. TPLs-fortified RS exhibited retarding of retrogradation as assessed by differential scanning calorimetry (DSC) and X-ray diffraction (XRD). The analytic samples had a water–RS/TPLs ratio of 2:1. RS/TPLs mixing ratios were 5/0, 5/0.3, 5/0.5, 5/0.7, and 5/1 (w/w) that equated with RS containing 0%, 6%, 10%, 14%, and 20% TPLs (based on RS weight), respectively. In the DSC analysis, the temperature and enthalpy of starch gelatinization obviously decreased as the polyphenols level increased. After storage at 4 °C, retrogradation enthalpy for RS with 10%, 14%, and 20% TPLs did not appear until storage of 20 days. After 10 days of storage at 4 °C, RS gel with 10%, 14%, or 20% TPLs had almost no recrystallization of the retrogradation. The overall results demonstrate that the marked inhibitory effect of TPLs on the retrogradation of RS.     

食品制造方法对膳食淀粉消化性能的影响(Ⅱ)——热加工

研究了3种不同的加热方式(煮沸、加压蒸煮、微波加热)对不同植物来源膳食淀粉消化性能的影响。结果表明:相比普通煮沸处理,加压蒸煮与微波加热对淀粉的消化性能影响较显著。加压蒸煮与微波加热使淀粉颗粒中直链淀粉浸析出来,淀粉糊化度增加,淀粉营养片断慢消化淀粉(SDS)与抗性淀粉(RS)大部分转化成易消化淀粉(RDS),消化指数指数达到80%左右,可降解淀粉营养片断与直链淀粉含量成负相关,与糊化度成正相关。不同热加工方法会影响淀粉中营养片断的比例。     

Effects of Physical Aging on Thermal and Mechanical Properties of Glassy Normal Corn Starch

Physical aging of glassy corn starch and its effect on the physical properties of compressed starch bars were investigated by using differential scanning calorimetry (DSC), dynamic mechanical thermal analyzer (DMTA), and Instron texturometer. Amorphous corn starch samples were prepared by drying a starch paste, followed by compressing the dried powders to bar‐type specimens under heat (100°C, 1.38×10⁷ Pa). These bars were then aged at 25°C for up to 22 days, after which thermal and mechanical characteristics were determined. There were progressive increases in glass transition temperature and relaxation enthalpy during the aging, which reached limiting values as structural equilibrium of the amorphous matrix was approached. The peak temperature of the relaxation endotherm (DSC thermogram) changed with time in a trend similar to the relaxation enthalpy. The storage modulus (DMTA) increased with aging time, but the peak intensity of tan δ decreased. The breaking strength (Instron texturometer) increased gradually with time of aging and reached a maximum. It has been demonstrated the relaxation kinetics for glassy amorphous corn starch can be characterized by both thermal and mechanical properties, such as relaxation enthalpy, storage modulus, and breaking strength.     

Development of starch-based peelable coating for edible packaging

This research characterised properties and stability of starch-based peelable coating film layers via mould dipping. Different ratios between rice starch (RS) and hydroxypropyl cassava starch (HS) containing agar blends were characterised for fluid rheology and properties of solid films. Layer-by-layer mould dipping in starch blend suspensions produced peelable shells. Rheology indicated that formability of thin-film layer on solid surface subsequently affected integrity of the shells. HS highly adsorbed water which plasticised matrices giving lower relaxation temperature (~10 °C) than RS and caused shrivelling and non-stable shell structures. RS increased crystallinity and non-homogeneity of films and reduced network flexibility and light transmission. IR spectra indicated modified hydrophobicity and hydrogen bonding of starch blends which governed water and oxygen permeability. Higher RS ratios significantly increased crystallinity which affected physical, mechanical and barrier properties during storage for three months. Findings indicated that HS provided flexibility and stability while RS improved formability of peeled shells.     

The influence of time and storage temperature on resistant starch formation from autoclaved debranched banana starch

Debranching and autoclaving processes of banana starch were carried out for obtaining a resistant starch-rich powder with functional characteristics. Debranching was carried out using pullulanase for 24 h and the autoclaving was done at 121 °C for 30 min, the samples were then cooled down and stored between 24 and 48 h, and temperatures between 4 and 60 °C. The resistant starch level increased due to the debranching and autoclaving processes. The water absorption index values decreased when the storage time increased, pattern that agrees with the higher RS content. The water solubility index (WSI) was affected by the storage temperature but not by the storage time. The autoclaved sample was hydrolyzed to a lesser extent than native starch. The RS-rich powder presented also crystallinity because the process of autoclaving and storage induced starch retrogradation. The procedure proposed might be used for production of a RS-rich powder from banana starch with high RS level and functional properties.     

Effect of maize type and extrusion‐cooking conditions on starch digestibility profiles

This study aimed at evaluating the influence of screw speed (250–600 rpm), barrel temperature (100–160 °C) and water content (16.4–22.5%) on rapidly digestible (RDS), slowly digestible (SDS) and resistant (RS) starch levels of waxy, normal and high‐amylose maize starches. In native starches, an increase in amylose content was correlated with lower SDS content. After extrusion, this trend was reversed. Both waxy and normal maize starches became rapidly digested. However, for normal maize starch, some SDS fraction remained. In contrast, the high‐amylose maize starch showed a significant increase in digestibility and an increase in SDS content from 20.4% in the native starch up to 27.5% after extrusion. This high level of SDS may be attributed to the presence of some remaining granular structures and formation of crystalline orders, which have slow digestion properties.     

Effect of Processing on Slowly Digestible Starch and Resistant Starch in Potato

The effect of a number of laboratory‐scale pretreatments on the proportions of rapidly digested (RDS), slowly digested (SDS) and resistant starch (RS) in raw and cooked potato has been examined using an in vitro digestion procedure. Potatoes of the variety Frisia were prepared in three states: raw, cooked, and cooked followed by a cold treatment (4°C, two days). Each preparation was then subjected in triplicate to freeze‐drying, coarsely mincing, pasting, freezing, dry‐milling after freeze‐drying, in 22 different combinations, before digesting. In raw potato, very little RDS and SDS (<5% total starch (TS)) were present, and the mechanical treatments of the potato did not affect the amounts of RDS and SDS. Cooking resulted in an almost complete conversion to RDS (>95% TS) in freshly‐cooked potato, but after post‐cooking cold treatment much of the RDS transformed to SDS, which reached a maximum of about 45% TS. SDS formation was independent of the degree of tissue disruption after cooking, and was generally associated with formation of RS, however, freezing after cooking allowed SDS formation without prolonged cold treatment and with very little associated RS (SDS 35% and RS 4% of TS). Freeze‐drying caused an increase in RS in most treatments of the cooked potatoes. The observed effects provided guidance for sample handling in potato research, but also suggested several approaches to the enrichment of SDS and/or RS, with a concurrent reduction in RDS, that could be used to improve the nutritional profile of potato products by decreasing RDS (lowered glycaemic impact), and increasing SDS (more sustained energy availability) and RS (prebiotic benefits).     

Study on retrogradation of maize starch–flaxseed gum mixture under various storage temperatures

This study aimed to investigate the effect of flaxseed gum (FG) on the retrogradation of maize starch (MS) gels under different storage temperatures. In this work, MS‐FG gels with 0–0.4% FG were stored under different temperatures (–20, 4 and 20 °C). Under all storage temperatures, the addition of FG inhibited the retrogradation process of MS, suppressed the recrystallisation of starch molecules, reduced the water loss of starch gels and improved the texture of the gels. Among the three storage temperatures, ?20 °C could almost stop the starch retrogradation but leading it results in the hardest gel texture. The retrogradation of MS‐FG gels stored under 4 °C was most serious with the fastest rate of recrystallisation. When stored at 20 °C, the gels had a retrogradation degree that was lower than when they were stored at 4 °C and also had the softest texture.