Effects of pre‐emulsifying fat/oil on meat batter stability,texture and microstructure

The effects of pre‐emulsified beef fat and canola oil (CO) (25%) with Tween 80 (T‐80) or sodium caseinate (SC) were studied in beef meat batters prepared at three protein levels (9%, 12% and 15%). Raising meat protein level to 15% resulted in low emulsion stability of products prepared with CO. Using pre‐emulsified beef fat with Tween 80 (BF‐T80) showed significantly higher fat and water losses at all protein levels. There were no differences in fat and water losses between pre‐emulsified beef fat and CO when SC was used at the 9% and 12% protein levels compared to the controls (non pre‐emulsification). Light microscopy revealed fat globule coalescence in the CO meat batters prepared with 15% protein and BF‐T8 treatments, as well as formation of fat channels and more protein aggregation; both resulted in lower emulsion stability. Using SC to emulsify fat/oil produced a finer dispersion of fat globules compared to all the other treatments.     

Influence of canola-olive oils, rice bran and walnut on functionality and emulsion stability of frankfurters

Fat content of frankfurters (20 g/100 g) was replaced with canola and canola-olive oils. Rice bran (RB) and walnut (WE) were added (2.5 g/100 g) to emulsions as macronutrients. Changes in energy values, color, emulsion stability and lipid oxidation of frankfurters during storage were investigated. ANOVA model was highly significant for color parameters and energy values (P < 0.001). The canola-olive oil replacement led to a high capacity to hold water and fat exudates in frankfurters, reporting higher emulsion stabilization parameters than regular frankfurters. The addition of RB led to an increase of cooking and fat exudates, indicating high emulsion instability possible due to interactions between RB fiber and fat-protein binders. Walnut addition reported low cooking loss values, and a significant capacity for emulsion stabilization in comparison with regular and RB frankfurters. Lipid oxidation increased from days 0-7 in all frankfurters, declining afterwards until end of storage. TBARS was not influenced by type of emulsions control, but significant (P < 0.05) differences were observed in vegetable oil emulsions made with RB; as well as between RB and WE added to either vegetable oil emulsions. These results suggest the use of these natural ingredients as valuable promoters of healthy meat products.     

Effect of Gradual Heating and Fat/Oil Type on Fat Stability,Texture, Color,and Microstructure of Meat Batters

The effects of endpoint cooking temperature (40, 50, 60, 70, 80, and 90 °C) on emulsion stability, texture, color, and microstructure of meat batters prepared with different fats/oils were studied. Canola oil treatments showed the highest cooking loss whereas hydrogenated palm oil provided the most stable meat batters. Rendered beef fat was less stable than regular beef fat. Increasing endpoint cooking temperatures resulted in a progressive reduction of water holding capacity in all treatments. As temperature was raised, meat batters showed higher hardness and cohesiveness values, but no appreciable changes in cohesiveness above 60 °C. Canola and hydrogenated palm oil treatments showed the highest hardness and chewiness values. Lightness (L^*) values of all meat batters increased significantly with increasing temperature from 40 to 60 or 70 °C; no major changes observed above 70 °C. Light microscopy revealed no substantial changes in the microstructure of all the stable meat batters cooked to between 50 and 70 °C. Heating to 90 °C changed the microstructure in all meat batters except the hydrogenated palm oil treatments, which still showed nonround fat particles and a less aggregated protein matrix.     

A Raman spectroscopic study of meat protein/lipid interactions at protein/oil or protein/fat interfaces

Structural changes in proteins of meat, FC (fat cream layers) and OC (soya bean oil cream layers) were studied by Raman spectroscopy. Results revealed that adsorption of proteins to the cream layers resulted in a maximum scattering change from 1657 to 1661 cm^?1, the intensity of amide I was significantly increased (P < 0.05), the percentages of secondary structures of proteins in the raw meat and meat cream layers were significantly different (P < 0.05), the bands near 540 and 475 cm^?1 were significantly (P < 0.05) higher in intensities in the soya bean oil cream layer samples compared to raw meat alone, the normalised intensity of the tryptophan band near 758 cm^?1, from 0.52 in the raw meat to 1.11 (fat cream layer) or 0.72 (soya bean oil cream layer). These results indicated that disulphide bonds, hydrophobic interactions and hydrogen bonding were of the main interactions observed in proteins and lipids at the emulsion interface.     

Partial substitution of pork fat with canola oil in Toscana sausage

The objective of this study was to develop and evaluate the effects of pork fat substitution with canola oil in Toscana sausage. Canola oil was incorporated into the product via lipid emulsion with water and collagen. The physicochemical and sensory parameters and fatty acid profiles were evaluated in five treatments with different canola oil concentrations (0, 2.5, 5.0, 7.5 and 10.0%). The prepared sausages met the legal identity and quality standards, with no significant differences detected in the variables moisture, ash content, lipid, pH, colour and weight loss due to cooking (P > 0.05). We observed a significant increase in the linolenic fatty acid content (omega-3 series). In the sensory evaluation, a significant difference was observed only for the texture variable. Pig fat substitution with 10% canola oil in the Toscana sausage exhibited the best physicochemical properties and fatty acid profile.Industrial relevanceVegetable oils have been used in meat products as an alternative to improve their lipid profiles. The amount of fat and the lipid profiles of meat products are the most important factors for product quality and consumer health. Our understanding of the relationship between the intrinsic food quality and health is gradually increasing, and as a result, a revolution has occurred in eating habits. Therefore, an innovative product for a healthier consumer market has been created to meet the challenges of animal fat substitution on the organoleptic characteristics of Toscana sausage.     

Influence of exopolysaccharide‐producing cultures on the volatile profile and sensory quality of low‐fat Tulum cheese during ripening

The objective of this investigation was to compare the composition and changes in the concentration of volatiles in low‐fat and full‐fat Tulum cheeses during ripening. Tulum cheese was manufactured from low‐ or full‐fat milk using exopolysaccharide (EPS)‐producing or non‐EPS‐producing starter cultures. A total of 82 volatile compounds were identified belonging to the following chemical groups: acids (seven), esters (21), ketones (14), aldehydes (six), alcohols (14) and miscellaneous compounds (20). The relative amounts of acids, alcohols and aldehydes increased in the cheeses made with EPS‐producing cultures during 90 days of ripening. Differences were found in the volatile profile of full‐fat Tulum cheese compared with the low‐fat variant, especially after 90 days of ripening. Exopolysaccharide‐producing cultures changed the volatile profile, and the EPS‐producing cultures including Streptococcus thermophilus + Lactobacillus delbrueckii subsp. bulgaricus + Lactobacillus helveticus (LF‐EPS2) produced cheese with higher levels of methyl ketones and aldehydes than the non‐EPS cultures. In the sensory analysis, full‐fat Tulum cheeses and the cheese produced with the EPS‐producing culture containing Lb. helveticus (LF‐EPS2) were preferred by the expert panel. It was concluded that the use of EPS‐producing starter cultures in the manufacture of low‐fat Tulum cheese had the potential to improve the flavour.     

Characteristics of low-fat meat emulsion systems with pork fat replaced by vegetable oils and rice bran fiber

The effects of vegetable oils prepared from olive, corn, soybean, canola, or grape seed, and rice bran fiber on the composition and rheological properties of meat batters were studied. Pork fat at 30% in the control was partially replaced by one of the vegetable oils at 10% in addition to reducing the pork fat to 10%. The chemical composition, cooking characteristics, texture properties, and viscosity of low-fat meat batters were analyzed. The moisture, protein, ash content, uncooked and cooked pH values, b^∗-value, hardness, cohesiveness, gumminess, chewiness, and viscosity of meat batters with vegetable oil and rice bran fiber were all higher than the control. In addition, batters supplemented with vegetable oil and rice bran fiber had lower cooking loss and better emulsion stability. Low-fat meat batters with reduced pork fat content (10%) and 10% vegetable oil plus rice bran fiber had improved characteristics relative to the regular fat control.     

Composition of phospholipid fraction in raw chicken meat and pre-cooked chicken patties: influence of feeding fat sources and processing technology

The effect of dietary lipid sources (animal fat, AF vs. vegetable oil, VO) was investigated on the phospholipid (PL) fraction of raw chicken meat and pre-cooked chicken patties. PL classes were determined on extracted lipids by high-performance liquid chromatography (HPLC) connected with an evaporative light-scattering detector (ELSD). The HPLC-ELSD method showed good intraday repeatability (less than 5% for different PL) and limits of detection ranging from 0.006 mg/mL (phosphatidylethanolamine) to 0.045 mg/mL (sphingomyelin). Diet did not consistently influence PL in chicken breast. A significant difference was assessed in meat batter where total PL were at levels of 349.0 and 388.2 mg/100 g of product in batter from broilers fed AF and VO diet, respectively. Cooking oil absorption caused an important decrease in PL (lower than 300 mg/100 g of product in pre-fried patties). Diet was an effective means to control the PL fatty acid profile in chicken, particularly the levels of polyunsaturated fatty acids (PUFA). In minced breast and thigh and meat batter from birds fed a VO-supplemented diet, the relative amount of PUFA was 46, 81 and 28% higher than that determined in the corresponding samples obtained from broilers fed an AF-based diet.     

Coconut Milk and Coconut Oil: Their Manufacture Associated with Protein Functionality

Coconut palm (Cocos nucifera L.) is an economic plant cultivated in tropical countries, mainly in the Asian region. Coconut fruit generally consists of 51.7% kernel, 9.8% water, and 38.5% shell. Coconut milk is commonly manufactured from grated coconut meat (kernel). Basically, coconut milk is an oil‐in‐water emulsion, stabilized by some proteins existing in the aqueous phase. Maximization of protein functionality as an emulsifier can enhance the coconut milk stability. In addition, some stabilizers have been added to ensure the coconut milk stability. However, destabilization of emulsion in coconut milk brings about the collapse of the emulsion, from which virgin coconut oil (VCO) can be obtained. Yield, characteristics, and properties of VCO are governed by the processes used for destabilizing coconut milk. VCO is considered to be a functional oil and is rich in medium chain fatty acids with health advantages.     

Influence of fat replacers on the chemical,textural and sensory properties of low‐fat Beyaz pickled cheese produced from ewe's milk

In this study, low‐fat Beyaz pickled cheeses were produced from ewe's milk using a protein‐based fat replacer (1.0% w/w Simplesse^®), a carbohydrate‐based fat replacer (0.5% w/w Maltrin^®) and a blend of both (0.5% w/w Simplesse^® + 0.25% w/w Maltrin^®). The chemical, textural and sensory properties of cheeses were examined during storage for 60 days. The use of fat replacers affected chemical (except pH and acidity), textural and sensory properties of the cheeses. The cheeses produced with Simplesse^® (Sample B) were similar to full‐fat cheese and had the highest sensory scores.     

Effects of Green Tea Extract and α‐Tocopherol on the Lipid Oxidation Rate of Omega‐3 Oils,Incorporated into Table Spreads,Prepared using Multiple Emulsion Technology

Abstract: This study examined the effectiveness of fat and water soluble antioxidants on the oxidative stability of omega (ω)‐3 rich table spreads, produced using novel multiple emulsion technology. Table spreads were produced by dispersing an oil‐in‐water (O/W) emulsion (500 g/kg 85 camelina/15 fish oil blend) in a hardstock/rapeseed oil blend, using sodium caseinate and polyglycerol polyricinoleate as emulsifiers. The O/W and oil‐in‐water‐in‐oil (O/W/O) emulsions contained either a water soluble antioxidant (green tea extract [GTE]), an oil soluble antioxidant (α‐Tocopherol), or both. Spreads containing α‐Tocopherol had the highest lipid hydroperoxide values, whereas spreads containing GTE had the lowest (P < 0.05), during storage at 5 °C, while p‐Anisidine values did not differ significantly. Particle size was generally unaffected by antioxidant type (P < 0.05). Double emulsion (O/W/O) structures were clearly seen in confocal images of the spreads. By the end of storage, none of the spreads had significantly different G′ values. Firmness (Newtons) of all spreads generally increased during storage (P < 0.05). Practical Application: Lipid oxidation is a major problem in omega‐3 rich oils, and can cause off‐odors and off‐flavors. Double emulsion technology was used to produce omega‐3 enriched spreads (O/W/O emulsions), wherein the omega‐3 oil was incorporated into the inner oil phase, to protect it from lipid oxidation. Antioxidants were added to further protect the spreads by reducing lipid oxidation. Spreads produced had good oxidative stability and possessed functional (omega‐3 addition) properties.     

W/O型人造肥牛脂肪的品质改进研究

人造肥牛脂肪是一种白色或乳白色黏稠状W/O型食品乳状液。在前期研究的基础上,通过在油相中添加牛油,应用油脂的同质多晶型特点及牛油的特殊组成来提高肥牛脂肪的稳定性;用一级大豆油与三级大豆油适当调配,来改善肥牛脂肪的颜色。实验结果表明,添加牛油的肥牛脂肪与未加牛油的相比乳化稳定性大大提高,可达到99.5%,而且脂肪的结构、质地、风味也得到改善;三级大豆油添加量为10%时,肥牛脂肪的黄度与亮度与天然牛油最接近。     

Physicochemical characteristics, textural properties and volatile compounds in comminuted sausages as affected by various fat levels and fat replacers

Physicochemical and textural properties, and volatile compounds of comminuted sausages with various added fat levels and fat replacers were evaluated. Sausages without added fat had higher expressible moisture and texture values, but their lightness and yellowness values were lower, as compared with sausages with 5%, 10%, or 15% fat. Increased fat levels with constant amounts of lean meat and added water increased the lightness and yellowness values, but tended the redness values tended to be similar. Low‐fat sausages (LFSs, <3%) containing non‐meat proteins, except egg white protein, had reduced texture values similar to those of regular‐fat counterparts (RFC). The addition of non‐meat proteins, except egg white protein, as components of fat replacers in LFS improved the textural characteristics, making them similar to those of RFC. LFSs containing sodium caseinate had reduced concentrations of pentadecanal and octadecanal, resulting in similar volatile compositions to those of RFC. Thus, sodium caseinate was the best non‐meat protein for the improvement of both texture and flavour of LFSs.     

Effects of protein level and fat/oil on emulsion stability,texture, microstructure and color of meat batters

Beef meat batters formulated with increasing protein level (10–15%) and containing 25% beef fat were compared to batters prepared with 25% canola oil. Emulsion stability of the canola oil treatments was higher (less separation during cooking) at the 10–13% protein level compared to the beef fat treatments. However, above 13% protein this was reversed and the canola oil treatments showed high fat and liquid separation, which did not occur at all in the beef fat treatments. This indicates differences in stabilization of fat versus oil in such meat emulsions. Hardness of the cooked meat batters showed significantly (P < 0.05) higher values when the protein level was raised, and was higher in canola oil than in beef fat meat emulsions at similar protein levels. Products’ chewiness were higher in the canola oil treatments compared to the beef fat emulsions. Lightness decreased and redness increased in canola oil batters as the protein level was raised. The micrographs revealed the formation of larger fat globules in the beef fat emulsions compared to the canola oil meat emulsions. The canola oil treatment with 14% protein started to show fat globule coalescence, which could be related to the reduced emulsion stability.     

采用METHOCEL MXTM技术开发低脂肪香肠和肉制品

由于消费者希望降低对脂肪特别是动物脂肪的摄取,诸如香肠类的肉制品已经逐步从消费者的日常饮食中降低使用量.METHOCELTMMX的脂肪替代技术可以使低脂肪的肉制品保持如全脂肉制品一样的优异的风味,提供良好的质构和柔嫩多汁的口感.这项技术使用健康的植物油来替代饱和脂肪酸或氢化植物油,形成形如"矩阵"的乳状液.将METHOCEL MXTM,冷水和植物油结合制成这个乳状液系统,植物油的含量可以是2%60%.本文中描述了一个用METHOCELTMMX的脂肪替代技术制作的10%脂肪含量的香肠,在保持良好质量的前提下,降低脂肪含量27%.     

POTENTIAL USES OF MECHANICALLY DEBONED BULLFROG (RANA CATESBEIANA) MEAT TO PARTIALLY REPLACE LEAN PORK TO PRODUCE EMULSIFIED MEATBALLS

ABSTRACT

The objective of this study was to evaluate the qualities of emulsified meatballs where part of the lean pork was substituted with mechanically deboned bullfrog (Rana catesbeiana) meat (MDBM) during manufacturing and subsequently stored at 4C. Meatballs were manufactured by adding 0, 7.5, 15, 22.5 or 30% MDBM. The meatballs had higher moisture, lower protein and fat contents, darker color, lower water‐holding capacity, higher cooking loss and softer texture as the MDBM usage level increased. The thiobarbituric acid and volatile basic nitrogen values of the products significantly (P < 0.05) increased, and the shear values, springiness and fracturability decreased as the MDBM usage level increased (P < 0.05). The products containing 7.5% MDBM had higher acceptable sensory characteristics than those samples containing more MDBM. In conclusion, emulsified meatballs that had 7.5% mechanically deboned bullfrog meat, substituted with equal quantities of the lean pork, were acceptable based on their physicochemical and sensory qualities.

PRACTICAL APPLICATIONS

Many animal by‐products which are produced during processing are edible and valuable. How to utilize these by‐products has become a great issue for the industry. In this study, high‐quality proteins which were obtained from mechanically deboned bullfrog meat (MDBM) resulted in emulsified meatballs with acceptable qualities. More specifically, this information could make MDBM a possible material to substitute for part of the added lean meat in many emulsion‐type meat products and increase products marketability because of lower raw ingredients' cost. This finding may be useful for both marine and domestic animal industries to make better use of this by‐product. Lastly, this information can be used to enhance the potential reduction of animal wastes, which might pollute the environment.

     

Physicochemical Effects of the Lipid Phase and Protein Level on Meat Emulsion Stability,Texture, and Microstructure

ABSTRACT: The effects of beef fat (25%) substitution with rendered beef fat, canola oil, palm oil, or hydrogenated palm oil at varying meat protein levels (8%, 11%, and 14%) were studied in emulsified beef meat batters. There was no significant difference in fat loss among meat batters made with beef fat, rendered beef fat, or palm oil. Hydrogenated palm oil provided the most stable batters at all protein levels. Increasing meat protein to 14% resulted in high fat loss in batters prepared with canola oil, which did not occur in the other formulations. This indicates that the physicochemical characteristics of fat/oil affect emulsion stability. Cooked batter hardness was higher (P < 0.05) when protein level was raised; highest in hydrogenated palm oil batters when compared at similar protein levels. As protein level was raised springiness values were increased in all the meat treatments. Springiness was higher in the canola oil treatments. Light microscopy revealed fat globule coalescence in canola oil meat batters prepared with 14% protein, as well as the development of fat channels and more protein aggregation; both seem to result in lower emulsion stability. Hydrogenated palm oil batters showed fat particles with sharp edges as opposed to the round ones seen in all other treatments.     

Production of squid emulsion sausages using pork skin and coconut powder mixture as fat replacers

How to improve the inferior properties of squid products is a big challenge for producers. Pork skin and coconut powder (PSCP) mixture were assessed as fat replacers in squid emulsion sausages. The modified treatment containing 100% PSCP had 62.58% less fat, 4.82% more protein and 8.79% more moisture than those of the control (P < 0.05). High PSCP concentration resulted in lower cooking loss, higher emulsion stability and better textural properties. Texture profile analysis (TPA) showed that the addition of PSCP significantly (P < 0.05) increased the hardness, cohesiveness and chewiness of the sausages. The three‐dimensional network structures of the sausages containing PSCP were more compact and homogeneous. Sensory evaluation results produced by a sensory panel comprised of 100 panellists indicated that the sausages had an excellent flavour and overall acceptability when the substitution was 60%. Therefore, pork skin and coconut powder can be used to replace the pork back fat in the squid emulsion sausage without sacrificing the quality.     

Studies on emulsion-type buffalo meat sausages incorporating skeletal and offal meat with different levels of pork fat

Ready-to-eat emulsion-type buffalo meat sausages were developed by using a combination of 80% meat components with 20% pork back fat. The meat components were constituted of 70 parts buffalo skeletal meat and 30 parts offal meat (rumen meat and heart meat in equal proportions). The emulsion stability, cooking losses of emulsions and sausages, composition of cooked sausages, eating quality of sausages and the microscopic characteristics of the raw emulsion and cooked sausages were studied. The light microscope micrograph of the raw emulsion showed uniformly well distributed fat globules embedded in a dense protein gel. The cooked emulsion also showed uniformly sized fat globules well distributed in a fine, compact, coagulated protein gel, which retained their original spherical shape. Good quality emulsion-type sausages could be produced having a high emulsion stability (0·87 ± 0·07 ml fat release/100 g emulsion); a low emulsion cooking loss (9·60 ± 0·60%) and a low sausage cooking loss (8·83 ± 0·48%). The overall acceptability of sausages was also high.     

Viscoelastic properties of acid milk gel as affected by fat nature at low level

The viscoelastic properties of acid milk gels containing small amounts of different fats were investigated. Skim milk was reconstituted from ultra low-heat skim milk powder and emulsions made with 2% (v/v) sunflower oil, olive oil, groundnut oil, or anhydrous milk fat using a pressure homogenizer. Acidification at 20 °C for 14 h to pH ∼4.6 was achieved by adding glucono-δ-lactone to the emulsion. Stress relaxation testing enabled determination of the firmness and the solid-like properties, i.e., elasticity. Regardless of the physical state of the fat, emulsion gels exhibited higher firmness than fat-free gels, despite the low fat level used. The firmness of the gels containing this small quantity of fat was more sensitive to temperature than was the firmness of fat-free gels. The relaxation time was higher in the presence of fat crystals. Modifications in the rheological properties of gels containing fat were attributed to fat droplets acting as active filler particles.