高效液相色谱法测定萘·吲可湿性粉剂

采用高效液相色谱法,使用反相色谱柱和紫外检测器测定了可湿性粉剂中萘乙酸和吲哚丁酸的含量。流动相为甲醇与水（５０／５０ ,Ｖ／Ｖ,ｐＨ３～４） 。方法的标准偏差分别为０－０１８ 和０－０５９,变异系数分别为０－００９％ 和０－０１１ ％ ,回收率分别为９９－８ ％ ～１００－８％ 和９９－１％ ～１００－２％ 。     

离子色谱荧光检测植物生长调节素吲哚-3-乙酸和吲哚-3-丁酸

利用离子色谱—荧光检测器法对吲哚-3-乙酸和吲哚-3-丁酸进行检测.该检测采用1.0mL/min的0.45V/V CH_3OH与0.01mol/L Na_2CO_3的混合液,利用Dionex OmniPac PAX-500柱分离,吲哚-3-乙酸和吲哚-3-丁酸的检测限分别为0.045ug/mL和0.167ug/mL,有良好的重现性和线性关系.对土壤样品进行测定,有较好的回收率.     

萘乙酸残留量的液相色谱法测定

在小麦、棉花、苹果等反复试验的基础上,建立了萘乙酸残留量定量检测的液相色谱法。样品经甲醇提取,过SPE C18小柱净化,采用150mm×4.6mm,C18不锈钢色谱柱,以甲醇+水+冰乙酸(体积比50∶50∶0.2)为流动相,流速为1.0mL/min,检测波长280nm,外标法定量进行HPLC测定。方法的线性范围在0.01～1.00mg/L,线性方程Y=3386.4X–105.96,相关系数0.9999;最小检出量为0.001ng,最低检出浓度0.001mg/kg,回收率为80.6%～101.2%。     

固相萃取-高效液相色谱-串联质谱法测定鲜人参中吲哚乙酸和吲哚丁酸

建立了固相萃取-高效液相色谱-串联质谱(SPE-HPLC-MS/MS)测定鲜人参中吲哚乙酸和吲哚丁酸含量的分析方法。样品经甲醇超声提取,HLB固相萃取小柱净化后,HPLC-MS/MS多反应监测模式测定,外标法定量。吲哚乙酸和吲哚丁酸在2~500μg/L范围内线性良好,相关系数均0.99,加标回收率为81.2%~95.6%,相对标准偏差为5.3%~10.6%,方法的定量限为10μg/kg。该方法灵敏、准确、可靠,能满足鲜人参中吲哚乙酸和吲哚丁酸定量分析的要求。     

α—萘乙酸高效液相色谱分析

本文采用Ｓｈｉｍ－ｐａｃｋＣＬＣ－ＴＭＳ柱，甲醇＋水为流动相，紫外检测器，外标法对α－萘乙酸进行了高效液相色谱分析研究。结果表明，α－萘乙酸含量测定的回收率在９９．２－１００．８％变异系数为０．４８％。     

高效液相色谱法测定水果及蔬菜中赤霉素残留

采用高效液相色谱方法,以甲醇-水(1%冰乙酸)为流动相,使用C18不锈钢柱,在254nm波长下,样品用80%甲醇进行提取,对赤霉素标样及果蔬中赤霉素含量分析测定。该分析方法的标准溶液在浓度为7.7～38.6μg.mL-1范围内呈良好线性,r值达到0.9997,检出限为0.5mg.kg-1,加标回收率为99.63%,精密度0.60%,该操作方法简单、快捷、精密度好、回收率高,适用于果蔬中赤霉素的残留分析。     

高效液相色谱法对丰产素中硝基酚类和α-萘乙酸的同时测定

用高效液相色谱法同时测定丰产素中硝基酚类和α-萘乙酸含量。色谱柱Shim-pack CLC-ODS(150×60mm,5μm),甲醇-水(用磷酸调至pH=3)(60 40)为流动相,紫外检测波长:280nm。灵敏度:0.16AUFS。流速:1.0 ml／min。5-硝基酚钠线性范围0.938-15.000 mg／100ml,平均回收率102.5％,精密度1.00％;对硝基酚钠线性范围0.813-45.000 mg／100ml,平均回收率104.3％,精密度0.62％;2,4.二硝基酚钠线性范围0.469-7.050 mg／100ml,平均回收率103.4％,精密度2.10％;邻硝基酚钠线性范围1.906-30.500 mg／100ml,平均回收率113.3％,精密度1.33％;α-萘乙酸线性范围3.750-60.000 mg／100ml,平均范围106.4％,精密度1.34％。此方法简便、灵敏、准确,可作为丰产素质量控制指标之一。     

反相高效液相色谱法测定吲达帕胺的含量

建立RP-HPLC法测定吲达帕胺含量的新方法.采用HPLC法,色谱柱为Kromasil C18柱;流动相中加入新的流动相-乙腈,V(水):V(乙腈):V(甲醇):V(冰醋酸)=65:17.5:17.5:0.1,流速1.0mL/min,检测波长240nm.该方法的线性范围为52.0～260.0μg/mL(r=0.9994),RSD=0.14%(n=6).本法简便、准确、灵敏、重现性好,适合吲达帕胺片的质量控制.     

高效液相色谱法同时测定酱油中的苯甲酸和山梨酸

探讨了采用高效液相色谱法(HPLC)同时测定酱油中的苯甲酸、山梨酸的分析方法。色谱条件:流动相为甲醇与乙酸铵溶液(0.02 mol/L),体积比为5∶95,流速1.0 mL/min,紫外检测波长230 nm,柱温25℃,进样体积10μL。采用外标法定量,在5~100μg/mL,苯甲酸、山梨酸回归方程相关系数>0.999。检出限分别为0.35μg/mL和0.32μg/mL,平均回收率分别为94.09%和98.15%,相对标准偏差(RSD)在2.09%~3.90%。实验结果表明,该方法准确、灵敏、操作方便。     

气相色谱法测定几种蔬菜水果中草甘膦残留

采用气相色谱法测定了苹果、桃、胡萝卜中革甘膦残留。通过阳离子柱净化,用三氟乙酸（TFA）、三氟乙酸酐（TFAA）和三甲基原甲酸酯（TMOA）进行衍生化反应,并在毛细管柱DB-17上分离后,ECD检测,质谱确证后,对其结果进行了分析。结果表明,该检测方法灵敏度高,最低检测限0．05mg／kg（S／N〉3）。平均添加回收率苹果93％、桃89％、胡萝卜92％,变异系数分别为10％、12％、10％。     

Determination of the antioxidant capacity of flavonoids in fruits and fresh and thermicaly treated vegetables

The main objective of the work was to determine the antioxidant capacity of the flavonoids in red apples with and without skin, strawberries, tomatoes and fresh onions and thermally treated: humid heat (boiled and vapor), dry heat (oven) and high frequency (microwaves). The measurement was carried out in a fluorescence spectroscopy and the statistical analysis through the Variance and Test of Duncan. The results indicate that the antioxidant capacity of the flavonoids of red apples with and without skin, strawberries, tomatoes and onions were of 0.259, 0.267, 0.278, 0.165 and 0.223 Equivalent uM Trolox. These values diminished after the thermal treatment at a higher degree by dry heat at 0.128, 0.072, 0.077 and 0.048 Equivalent uM Trolox respectively and 0.146 Equivalent uM Trolox in the boiled onion. The flavonoids showed a higher antioxidant activity in fresh state, and their activity diminished under heat (cooking).     

Antioxidant capacity of fruits and vegetables cultivated in Chile

The high prevalence of non transmissible chronic diseases (NCD) related to food consumption had increased the studies conducted to investigate the relationship between diet and health. A smaller incidence of NCD, with food patterns with high consumption of fruits and vegetables has been observed and chemical compounds of these foods have been one of the main subjects of the actual research in the reaqltion between food consumption and health. The effect of vegetable foods has been attributed to various nutrients and bioactive compounds with antioxidant activity. In order to determine the antioxidant capacity of vegetable foods cultivated in Chile, natural fruits and vegetables were analyzed according to the FRAP (ferric reducing activity power) method, reading to the 4 minutes. In vegetables, the values were between 0.002 and 1.91 milimoles of Fe/l00 g for cooked carrot and red pepper respectively. The values of the fruits ranged between 0.02 milimoles of Fe/100 g for the cucumber and 12.32 for maqui, the berries studies showed values between 3.10 for strawberry and 3.55 for wild blackberry. Lemmon and quince with 0.25 and 0.23 respectively are located in the intermediate level and the lowest values within the fruits corresponded to apple (fuji variety) and peaches.     

健康型蔬菜水果清洁剂的研制

本实验发明了一种化合物组合：新型乳化剂、天然植物碱、纳米降解剂、助溶剂、天然香型和水等组成。通过试验筛选出最佳配方，并且测定了该清洁剂的去污能力、杀菌能力、对残留农药降解效果、泡沫性能、表面张力和稳定性。结果表明，该产品制备简便、综合洗洁性能好，具有较大市场前景。     

果蔬微波联合干燥技术研究进展

介绍了微波干燥的原理和特点，综述了果蔬微波联合干燥技术的研究进展，主要涉及到微波干燥与其它干燥技术联合在果蔬干燥中的应用。     

试纸法快速检测果蔬中敌敌畏含量的研究

基于比色法原理,以定量滤纸为纸基,以间苯二酚溶液为显色剂,研制出一种快速检测果蔬中有机磷农药敌敌畏的试纸。结果表明,此方法的检出限为12.5 mg/kg,检测时间仅需5 min。该试纸法具有成本低、耗时短、操作简单、携带方便等优点,适合于果蔬中敌敌畏残留的现场初步筛查。     

试纸法快速检测果蔬中敌敌畏含量的研究

基于比色法原理,以定量滤纸为纸基,以间苯二酚溶液为显色剂,研制出一种快速检测果蔬中有机磷农药敌敌畏的试纸。结果表明,此方法的检出限为12.5 mg/kg,检测时间仅需5 min。该试纸法具有成本低、耗时短、操作简单、携带方便等优点,适合于果蔬中敌敌畏残留的现场初步筛查。     

茁霉多糖在果蔬保鲜应用的研究

茁霉多糖是出芽短梗霉产生的细胞外多糖,一般都一蓝黑色素伴随着茁霉多糖一起产生,因此,出芽短梗霉俗称黑酵母。茁霉多糖一种无色无味的中性多糖,极易溶于水。具有安全性,无毒性,耐热性,耐酸碱性,耐盐性,粘性,可塑性,成膜性质等特性,具有广泛的应用前景。本实验将茁霉多糖制成茁霉多糖膜溶液,进行果蔬保鲜的研究。     

Cholesterol binding capacity of fiber from tropical fruits and vegetables

The cholesterol binding capacity of 28 fiber samples from a variety of the more common tropical fruits and vegetables was determined. The binding capacity of cholestyramine, cellulose, lignin, guar gum and citrus pectin were also determined. Capacities were evaluated by an in vitro method that simulates the effect of the human digestive system on fiber using a series of enzymatic treatments before the binding was determined. Binding values varied from 3% for a soluble fraction of cassava to 84% for cholestyramine. Values for most fruit and vegetable fiber samples were less than or ca. equal to cellulose or lignin (20% and 16%, respectively). Apart from cholestyramine, sweet potato was the most effective binder (30%). Citrus pectin, at 8%, was a relatively poor binder. The capacity of guar gum (17%) was slightly less than cellulose. These data do not support the conclusion from in vivo studies that the hypocholesteremic effects observed for citrus pectin and guar gum are the result of the direct binding of cholesterol or bile acids in the large intestine. Southern Region, U.S. Department of Agriculture, Agricultural Research Service. Mention of a Trademark or proprietary product is for identification only and does not imply a guarantee or warranty of the product by the U.S. Department of Agriculture over other products that may also be suitable.