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The influence of dietary simvastatin, cholestyramine, and the combination of simvastatin plus cholestyramine on hepatic cholesterol
metabolism has been investigated in male rats. Recovery from the effects of the drugs was also investigated by refeeding normal
chow for 24 h. Both drugs, alone and in combination, increased 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase activityin vitro, but activity returned toward control values, after drug withdrawal. Acyl-CoA:cholesterol acyltransferase (ACAT) was significantly
reduced (P<0.001) by simvastatin (−75%), cholestyramine (−71%), and by the drug combination (−81%), due both to a decrease in microsomal
cholesterol and to nonsubstrate-dependent modulation of enzyme activity. Refeeding control diet increased ACAT activity but
not to control levels. The enhanced activity arose partly from higher microsomal cholesterol and partly from increases in
total enzyme activity. Cytosolic neutral cholesteryl ester hydrolase (CEH) activity was substantially elevated by simvastatin
(3-fold) and by the drug combination (6-fold), whereas the effect of cholestyramine was smaller (1.5-fold). Normal chow for
24 h only partially returned cytosolic CEH activity to control values. Microsomal CEH activity was increased by simvastatin,
alone and in combination with cholestyramine (1.4 to 1.7-fold), and was also enhanced, in the cholestyramine-treated animals,
following drug withdrawal. Removal of simvastatin did not allow recovery of this enzyme activity, while withdrawal of the
drug combination led to values 29% below controls. The results indicate that in the rat, simvastatin and cholestyramine alter
both ACAT and CEH activity, as well as inhibiting HMG-CoA reductase activity. 相似文献
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《Food and cosmetics toxicology》1980,18(4):363-366
The uptake, metabolism and excretion of betanin from beetroot extract were studied in the rat after iv injection or peroral administration in vivo, and in the isolated perfused liver in vitro. When injected iv, betanin was almost completely recovered in the urine. When given orally only 3% was recovered in the urine and 3% in the faeces after 24 hr. In the isolated perfused liver very little betanin was found in the bile, and most of the added betanin was recovered from the perfusion fluid. In studies in vitro it was found that betanin was largely metabolized by the tissues lining the gastro-intestinal tract. It is concluded that orally-administered betanin is poorly absorbed, and that the majority of it is metabolized in the gut. Betanin injected iv had effects on the cardiovascular system, transiently increasing the blood pressure and heart rate. It also increased the magnitude of the contractions of an isolated portal vein, in vitro. Both the in vivo and the in vitro effects could be partially blocked by specific adrenergic and cholinergic blockers, but the mechanism of the cardiovascular effects of betanin remains to be determined. 相似文献
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Linoleic and arachidonic acids, labeled with14C and injected intratesticularly, were used to study with time the interconversion of polyunsaturated fatty acids in rat testis
and their incorporation into the major lipid classes. With both substrates14C activity was readily incorporated into longer chain, more highly unsaturated fatty acids. After the injection of 1-14C-linoleic acid the major portion of the14C was found in palmitic, linoleic, 8,11,14-eicosatrienoic, 5,8,11,14-eicosatetraenoic, 7,10,13,16-docosatetraenoic and 4,7,10,13,16-docosapentaenoic
acids. Hydrogenation of the total fatty acids isolated from rat testes after intratesticular injection of 1-14C-linoleate revealed that the polyenoic acids hydrogenating to lignoceric acid (previously characterized as 9,12,15,18-tetracosatetraenoate
and 6,9,12,15,18-tetracosapentaenoate) had a relatively high specific activity. After the injection of 1-14C-arachidonate significant14C activity was found in palmitate, 7,10,13,16-docosatetraenoate, 4,7,10,13,16-docosapentaenoate, 9,12,15,18-tetracosatetraenoate
and 6,9,12,15,18-tetracosapentaenoate. The biosynthesis of the ω6 polyunsaturated fatty acids in rat testis is discussed in relation to these data. Investigation of the distribution of label
in the complex lipid fractions demonstrated the majority of the14C activity to be present in phosphatides and triglycerides after injection of either of these14C substrates with only small quantities being present as nonesterified acids. At the time periods studied the polyenoic acids
of triglycerides had a higher specific activity than the corresponding acids of phosphatides with the exception of linoleate.
Presented in part at the Meeting of the American Institute of Nutrition, Atlantic City, April 1968 and at the AOCS Meeting
in New York, April 1969. These data were taken from a thesis submitted by R. B. Bridges in partial fulfillment of the requirements
for the Ph.D. degree, Vanderbilt University. 相似文献
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Treatment of neonatal rats with U18666A, an inhibitor of desmosterol Δ24-reductase, results in accumulation of desmosterol (Δ5,24) and depletion of cholesterol (Δ5) in various bodily tissues and also causes cataracts. We evaluated the effects of U18666A on the sterol composition, de novo sterol synthesis, and histological structure of the retina. Neonatal Sprague-Dawley rats were injected subcutaneously with
U18666A (15 mg/kg, in olive oil) every other day from birth through 3 wk of age; in parallel, control rats received olive
oil alone. At 21 d, treated and control groups each were subdivided into two groups: one group of each was injected intravitreally
with [3H]acetate; retinas were removed 20 h later and non-saponifiable lipids (NSL) were analyzed by radio-high-performance liquid
chromatography. The other group was injected intravitreally with [3H]leucine; 4 d later, one eye of each animal was evaluated by light and electron microscopy and light microscopic autoradiography,
while contralateral retinas and rod outer segment (ROS) membranes prepared thereform were analyzed by sodium dodecyl sulfate-polyacrylamide
gel electrophoresis/fluorography. In the treated group, the Δ5/Δ5,24 mole ratio of retinas was ca. 1.0, and >88% of the NSL radioactivity was in Δ5,24; in contrast, control retinas had Δ5/Δ5,24 >170, with >80% of the NSL radioactivity in Δ5. Retinal histology, ultrastructure, ROS renewal rates, and rhodopsin synthesis and intracellular trafficking were comparable
in both treated and control animals. These results suggest that desmosterol can either substitute functionally for cholesterol
in the retina or it can complement subthreshold levels of cholesterol by sterol synergism. 相似文献
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Reportedly’ randomly rearranging the position of fatty acids (FA) in butterfat triacylglycerol (TAG) by interesterification’
thereby lowering the proportion of saturated FA in the sn-2 position’ reduces its hypercholesterolemic and hypertriglyceridemic properties when fed to humans. The aim of this work
was to determine if these reductions in plasma cholesterol and TAG could be explained by an improved rate of clearance from
the plasma of chylomicrons composed of randomized butterfat’ using a rat model. Acute chylomicron clearance studies demonstrated
no differences in fractional clearance rates of cholesteryl esters and TAG from the plasma of rats infused with chylomicrons
produced from gastric feeding of either native (NBF) or randomized (RBF) butterfat. Although there was a 14% decrease in the
level of saturated FA occupying the sn-2 position of TAG in RBF compared with NBF’ this difference became negligible (∼5%)’ following digestion of the fat and subsequent
repackaging of TAG into chylomicrons. These observations suggest that the previously observed reduction in hypercholesterolemic
properties of randomized butterfat in rat is unlikely to be explained by improved clearance of chylomicron TAG. 相似文献
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在浓盐酸中由环己酮和2,6-二甲基苯胶一步合成了多取代基的4,4'-二胺基-2,2',6,6'-四甲基二苯基环己烷。报道了该二胺的纯化,井使用元素分析、红外光谱、核磁共振谱等分析手段对其进行了表征。 相似文献
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A total synthesis ofdl-phosphatidyl-(dilinoleoyl)-l-serine was achieved by the acylation of the barium salt of the phthalimidomethyl ester of glycerophosphoryl-n-anisyloxycarbonyl-l-serine. The dilinoleoyl intermediate was treated with hydrazine to remove the phthalimidomethyl group and with hydrogen chloride
to remove the anisyloxycarbonyl protecting group. The resulting phosphatidylserine was purified by Rouser's methods, solubilized,
and tested for biological activity in the antithromboplastin, recalcification, and Hicks-Pitney tests. It was found to have
about the same anticoagulant activity as beef brain phosphatidylserine and hence was more active than the less unsaturated
phosphatidylserine synthesized earlier.
An abstract of the material in this paper appeared in Fed. Proc.24, No. 727 (1965). 相似文献
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Marc J. Van Rafelghem John P. Vanden Heuvel Lawrence A. Menahan Richard E. Peterson 《Lipids》1988,23(7):671-678
Alterations in lipid metabolism were axamined in adult male Sprague-Dawley rats seven days after a single intraperitoneal
injection of perfluorodecanoic acid (PFDA; 20, 40 or 80 mg/kg). Because PFDA treatment caused a dose-related reduction in
feed intake, the response of vehicle-treated rats pair-fed to those receiving PFDA was monitored to distinguish direct effects
of the perfluorinated fatty acid from those secondary to hypophagia. Carcass content of lipid phosphorus and free cholesterol
decreased in dose-dependent fashion in both PFDA-treated and pair-fed rats. Carcass triacylglycerols diminished in a similar
manner, yet PFDA-treated rats at each dose had a higher concentration of neutral acylglycerols than their vehicle-treated,
pair-fed counterparts. In vehicle-treated, pair-fed rats at the 80 mg/kg dose level, lipid phosphorus and free cholesterol
as a proportion of carcass fat increased, whereas the share of the triacyl-glycerols declined. Because of the higher concentration
of triacylglycerols in the carcass of rats treated with 80 mg/kg PFDA, enrichment of lipid phosphorus and free cholesterol
in carcass fat was less than in their pair-fed partners. The amount of lipid phosphorus and free cholesterol per hepatocyte
was similar in both PFDA-treated rats and their pair-fed partners. Liver triacyl-glycerols were markedly increased in PFDA-treated
rats. A similar but less extensive augmentary effect of PFDA on hepatic esterified cholesterol was found. Concentration of
triacylglycerols in plasma was not elevated in PFDA-treated rats, in spite of hepatic accumulation of esterified compounds.
Also, the plasma level of free fatty acids and 3-hydroxybutyrate was similar in all treatment groups, including those receiving
PFDA. Thus, the administration of PFDA appears to divert fatty acids from oxidation toward esterification in the liver. 相似文献
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The fate of pristane (2,6,10,14-tetramethylpentadecane), a widespread isoprenoid hydrocarbon, has been studied in rats after
a single per os administration of3H-labeled pristane. The balance study showed an extensive fecal excretion (66%) mainly as unchanged hydrocarbon, whereas about
14% of ingested pristane was excreted in urine as pristane metabolites and tritiated water. After one wk, 8.3% of the ingested3H still was stored in the carcass, and radioactive distribution in tissues and organs showed a preferential incorporation
into adipose tissue and liver. Over 75% of the radioactivity stored in the carcass was associated with pristane metabolites
and tritiated water. Tissue metabolites were characterized by thin layer chromatography, gas chromatography and mass spectrometric
analyses. Four metabolites were identified: pristan-1-ol, pristane-2-ol, pristanic acid and 4,8,12-trimethyltridecanoic acid.
These demonstrate that this isoprenoid hydrocarbon undergoes subterminal hydroxylation or terminal oxidation followed by the
classical β-oxidation process. Incorporation of metabolites in phospholipids and more particularly in the phosphatidylserine
fraction has been observed and is discussed. 相似文献
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Male and female rats were fed diets containing 2% of calories as corn oil or that plus 40% of calories as beef tallow or corn
oil. After 3, 6, 12 and 18 months groups were given 4-14C-cholesterol ip, and feces were collected for 9 days. Just prior to necropsy3H-acetate was administered ip. Samples of serum, liver, heart and carcass were obtained for analysis. Concentrations of fatty
acids and cholesterol, synthesis of those and recovery of ring-labeled steroid are reported. Mortality from acute respiratory
disease was very high in male rats fed beef tallow or low fat diets and very low in those fed the corn oil diet. In females,
only beef tallow diet resulted in a high mortality rate, and this was lower and at a later age than in males. The most notable
effects of age were in relation to fatty acid synthesis and presence of14C-acidic steroid in the carcass. In 3-month-old rats both fats depressed fatty acid synthesis in comparison to the low fat
diet. At later ages beef fat ceased to depress fatty acid synthesis in both sexes. Corn oil continued to depress fatty acid
synthesis up to 12 months in males and 18 months in females. The presence of14C-acidic steroid in carcass was substantial in 6-month-old rats and constituted ca. 40% of recovered14C in 18-month-old rats. The possibility that the increase in acetate incorporation into fatty acids with age in fat feeding
is related to chain elongation rather than de novo synthesis is discussed. Both the presence and amount of acidic steroid
in the carcass are notable and may be of importance in constructing models of cholesterol turnover.
Presented in part at the AOCS Sterol Symposium, April 1970, and the Federation of American Societies for Experimental Biology,
April 1971.
Scientific Series Paper No. 1536, Colorado Agricultural Experiment Station. 相似文献
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The metabolism of [1-14C]linoleic, [1-14C]arachidonic and [3-14C]docosa-4,7,10,13,16-pentaenoic acids was investigated after intratesticular injection of the labeled compounds and isolation
of rat Sertoli and germinal cells. Following injection of either14C-linoleate or14C-arachidonate, the specific activity (sp act) of docosa-4,7,10,13,16-pentaenoic acid of Sertoli cells was greater than that
of the germinal cells. The data suggest that the Sertoli cells are more active in the biosynthesis of the 22-carbon pentaene
than the germinal cells. Differences between these 2 cell types were also noted in the distribution of the incorporated14C among the various lipid classes. Following intratesticular injection of14C-docosapentaenoic acid, a greater proportion of the recovered14C in Sertoli cells than in germinal cells was present in 20-carbon fatty acids, suggesting a greater activity in Sertoli cells
in the metabolism of the pentaene. The major portion of the recovered14C in both cell types was present in triacylglycerols during early time periods and in phospholipids after 24 hr. The possibility
of transfer of biosynthesized docosapentaenoic acid from Sertoli to germinal cells is discussed. 相似文献