NIR Laser-Triggered Microneedle-Based Liquid Band-Aid for Wound Care

Microneedles (MNs) have attracted widespread scientific and industrial interest in the past decade as an efficient, painless, low-cost, and relatively safe transdermal drug delivery device. However, their drawbacks such as insufficient dose accuracy and limited penetration depth may limit the clinical applications. Here, a light-controlled liquid band-aid based on MNs is developed for antibacterial applications. Metal–organic framework-derived peroxidase-like nanozyme loaded in MNs can not only convert light energy into heat to enhance drug permeation but also decompose hydrogen peroxide into hydroxyl radicals for antibacteria. The heat generated by the nanozyme can facilitate MNs to melt and form a liquid band-aid, which is beneficial to insulate the wound from the surrounding bacterial environment. These studies in a Staphylococcus aureus-infected mice model also prove that this laser-triggered liquid band-aid can efficiently reduce skin inflammation and promote wound healing. Together, these results demonstrate that the rational design of MNs can enhance antibacterial and wound healing efficiency.     

Labeling of Adipose‐Derived Stem Cells by Oleic‐Acid‐Modified Magnetic Nanoparticles

The in vivo tracking of adipose derived stem cells (ASCs) is of essential concern when they are used as seed cells in tissue engineering. This study explores the feasibility of using magnetic nanoparticles (MNs), a type of contrast agents in magnetic resonance imaging (MRI), to label ASCs such that the labeled ASCs could be tracked in vivo by MRI non‐invasively and repeatedly. To do this, MNs of <10 nm surface‐coated with oleic acid are synthesized via a high‐temperature solution‐phase reaction. Cytotoxicity of the as‐synthesized MNs at concentrations up to 0.1 mg mL^?1 on 10⁴ cells mL^?1 ASCs is evaluated by LDH release. Since only minor cytotoxicity is detected, the effects of the labeling technique on cellular behaviors and uptake by labeled cells are investigated. Cell proliferation and differentiation with and without MNs are compared. The results show that proliferation of ASCs (10⁴ cells mL^?1) labeled by MNs (0.05 mg mL^?1) is significantly enhanced and dependent on the labeling time. The MNs are located in the vesicles within cytoplasm of ASCs. The cellular uptake reaches as high as ～180 pg/cell. Nevertheless, the labeled ASCs still maintained adipogenic and osteogenic differentiation. Hence, the feasibility of labeling ASCs by oleic acid coated MNs is ascertained and it was better to label the cells during their quiescent stage. The labeled ASCs can also be in vivo detected by MRI in a subcutaneous model in vivo. Further MRI tracking of the labeled ASCs in long‐term follow‐up would thus follow this current study.     

Reliability and performance analysis of a modular multistage interconnection network

This paper introduces a class of cost-effective fault-tolerant multistage interconnection networks known as modular networks (MNs). With the help of reliability and cost analysis, we showed that MNs perform more reliably and are more cost-effective than most other previously proposed fault-tolerant networks. Performance analysis of the MNs has also been carried out under both fault-free and fault-present conditions; we showed that MNs provide a higher probability of acceptance than those of their counterpart unique-path networks and that the performance does not degrade too much in the presence of faults.     

A mobility-based load control scheme in Hierarchical Mobile IPv6 networks

By introducing a mobility anchor point (MAP), Hierarchical Mobile IPv6 (HMIPv6) reduces the signaling overhead and handoff latency associated with Mobile IPv6. In this paper, we propose a mobility-based load control (MLC) scheme, which mitigates the burden of the MAP in fully distributed and adaptive manners. The MLC scheme combines two algorithms: a threshold-based admission control algorithm and a session-to-mobility ratio (SMR)-based replacement algorithm. The threshold-based admission control algorithm gives higher priority to ongoing mobile nodes (MNs) than new MNs, by blocking new MNs when the number of MNs being serviced by the MAP is greater than a predetermined threshold. On the other hand, the SMR-based replacement algorithm achieves efficient MAP load distribution by considering MNs’ traffic and mobility patterns. We analyze the MLC scheme using the continuous time Markov chain in terms of the new MN blocking probability, ongoing MN dropping probability, and binding update cost. Also, the MAP processing latency is evaluated based on the M/G/1 queueing model. Analytical and simulation results demonstrate that the MLC scheme outperforms other schemes and thus it is a viable solution for scalable HMIPv6 networks.     

Antitumor Platelet‐Mimicking Magnetic Nanoparticles

Nanoparticles possess the potential to revolutionize cancer diagnosis and therapy. The ideal theranostic nanoplatform should own long system circulation and active cancer targeting. Additionally, it should be nontoxic and invisible to the immune system. Here, the authors fabricate an all‐in‐one nanoplatform possessed with these properties for personalized cancer theranostics. Platelet‐derived vesicles (PLT‐vesicles) along with their membrane proteins are collected from mice blood and then coated onto Fe₃O₄ magnetic nanoparticles (MNs). The resulting core–shell PLT‐MNs, which inherit the long circulation and cancer targeting capabilities from the PLT membrane shell and the magnetic and optical absorption properties from the MN core, are finally injected back into the donor mice for enhanced tumor magnetic resonance imaging (MRI) and photothermal therapy (PTT). Meanwhile, it is found that the PTT treatment impels PLT‐MNs targeting to the PTT sites (i.e., tumor sites), and exactly, in turn, the enhanced targeting of PLT‐MNs to tumor sites can improve the PTT effects. In addition, since the PLT membrane coating is obtained from the mice and finally injected into the same mice, PLT‐MNs exhibit stellar immune compatibility. The work presented here provides a new angle on the design of biomimetic nanoparticles for personalized diagnosis and therapy of various diseases.     

Self-Powered Controllable Transdermal Drug Delivery System

Traditional topical ointment applied on the skin surface has poor drug penetration due to the thickening of the stratum corneum for psoriasis. Microneedles (MNs) provide a desirable opportunity to promote drug penetration. However, the common MNs are difficult to meet the requirement of on-demand drug delivery. In this study, a smart electrical responsive MNs is fabricated by introducing conductive material of polypyrrole (PPy). Further, a self-powered controllable transdermal drug delivery system (sc-TDDS) based on piezoelectric nanogenerator (PENG) is developed. The sc-TDDS can control drug release by collecting and converting mechanical energy into electrical energy. The sc-TDDS can release 8.5 ng dexamethasone (Dex) subcutaneously per electrical stimulation. When treating psoriasis-like skin disease with sc-TDDS, the inflammatory skin returned to normal after 5 days, which is obviously better than treating with traditional Dex solution coating. This work provides a promising approach of on-demand transdermal drug release for various disease treatment scenarios.     

Engineered Microneedles for Interstitial Fluid Cell‐Free DNA Capture and Sensing Using Iontophoretic Dual‐Extraction Wearable Patch

Interstitial fluid (ISF), as an emerging source of biomarkers, is unmistakably significant for disease diagnosis. Microneedles (MNs) provide a minimally invasive approach for extracting the desired molecules from ISF. However, existing MNs are limited by their capture efficiency and sensitivity, which impedes early disease diagnosis. Herein, an engineered wearable epidermal system is presented with a combination of reverse iontophoresis and MNs for rapid capture and sensing of Epstein‐Barr virus cell‐free DNA (an important biomarker of nasopharyngeal carcinoma). Owing to a dual‐extraction effect demonstrated by reverse iontophoresis and MNs, the engineered wearable platform successfully isolates the cell‐free DNA target from ISF within 10 min, with a threshold of 5 copies per µL and a maximum capture efficiency of 95.4%. The captured cell‐free DNA is also directly used in a recombinase polymerase amplification electrochemical microfluidic biosensor with a detection limit of 1.1 copies per µL (or a single copy). The experimental data from immunodeficient mouse models rationalizes the feasibility and practicality of the wearable system. Collectively, the developed method opens an innovative route for minimally invasive sampling of ISF for cell‐free DNA‐related cancer screening and prognosis.     

Cancer Stem Cell‐Platelet Hybrid Membrane‐Coated Magnetic Nanoparticles for Enhanced Photothermal Therapy of Head and Neck Squamous Cell Carcinoma

Cell membrane–based nanosystems with desirable characteristics have been studied extensively for many therapeutic applications. However, current research has focused on single cell membrane, and multifunctional fused membrane materials from different membrane types are still rare. Herein, a platelet–cancer stem cell (CSC) hybrid membrane‐coated iron oxide magnetic nanoparticle (MN) {[CSC‐P]MN} is presented for the first time for the enhanced photothermal therapy of head and neck squamous cell carcinoma (HNSCC). Inherited from the original source cells, the platelet membrane shows immune evading ability due to the surface marker comprising a number of “don't eat me” signals, and the CSC membrane has homotypic targeting capabilities due to the specific surface adhesion molecules. The [CSC‐P]MNs possess superior characteristics for immune evasion, active cancer targeting, magnetic resonance imaging, and photothermal therapy. Compared with single cell membrane–coated MNs, [CSC‐P]MNs exhibit prolonged circulation times and enhanced targeting abilities. Moreover, the [CSC‐P]MNs exhibit a superior photothermal ability that provides excellent HNSCC tumor growth inhibition, particularly in an immunocompetent Tgfbr1/Pten conditional double knockout HNSCC mouse model that contains a more complex tumor microenvironment that is similar to the human HNSCC microenvironment. Collectively, this biomimetic multimembrane‐coated nanoplatform may provide enhanced antitumor efficacy in the complex tumor microenvironment.     

Scheme for improving transmission performance of realtime traffic using priority queues in HMIPv6

Hierarchical Mobile IPv6 (HMIPv6) has been proposed by the Internet Engineering Task Force (IETF) to reduce handoff latency and signaling overhead. In the new protocol, Mobility Anchor Point (MAP) receives all packets in place of Mobile Node (MN) and MAP services are transferred to Care of Address (CoA) of MN. However, a MAP may be a single point of performance bottleneck because the MAP should not only handle signaling traffic but also process data tunneling traffic for all MNs registered in a MAP domain. So, MAPs need MAP management scheme for the multimedia services or real time services. We propose a MAP selection scheme that can select different MAPs according to the traffic characteristics of MNs and a multilevel queue processing method that can process binding updates based on the priorities of queues in a MAP when MNs send BU. Quantitative results of the performance analysis show that our proposal can reduce the location update cost by 31% and the total cost by 14%. With the multilevel queues, we could reduce the total cost by 12% and 17% for D=4 and D=8 respectively.     

An enhanced fast handover triggering mechanism for Fast Proxy Mobile IPv6

Handover delay performance is a critical issue to support real-time applications in wireless networks. To address this issue, this paper presents an Enhanced fast handover Triggering Mechanism (ETM) to improve the handover performance of mobile nodes (MNs) in Fast Proxy Mobile IPv6 (FPMIPv6). Making use of the information from the link layer, the ETM predicts two cases that the MNs perform in the reactive handover mode. Then, it establishes the bi-directional tunnel in advance for fast handover. As a result, the reactive handover delay is significantly reduced. Integrating the ETM into FPMIPv6 forms an enhanced Fast Proxy Mobile IPv6 (eFPMIPv6) protocol. Simulation experiments show that with the presented ETM mechanism, the eFPMIPv6 outperforms the original FPMIPv6 in terms of the overall handover performance.     

Efficient Drug Delivery into Skin Using a Biphasic Dissolvable Microneedle Patch with Water-Insoluble Backing

Dissolvable microneedle patches (MNPs) enable simplified delivery of therapeutics via the skin. However, most dissolvable MNPs do not deliver their full drug loading to the skin because only some of the drug is localized in the microneedles (MNs), and the rest remains adhered to the patch backing after removal from the skin. In this work, biphasic dissolvable MNPs are developed by mounting water-soluble MNs on a water-insoluble backing layer. These MNPs enable the drug to be contained in the MNs without migrating into the patch backing due to the inability of the drugs to partition into the hydrophobic backing materials during MNP fabrication. In addition, the insoluble backing is poorly wetted upon MN dissolution in the skin, which significantly reduces drug residue on the MNP backing surface after application. These effects enable a drug delivery efficiency of >90% from the MNPs into the skin 5 min after application. This study shows that the biphasic dissolvable MNPs can facilitate efficient drug delivery to the skin, which can improve the accuracy of drug dosing and reduce drug wastage.     

Load balancing and its performance evaluation for layer 3 and IEEE 802.21 frameworks in PMIPv6‐based wireless networks

Mobile IP (MIP) requires mobile nodes (MNs) to register with the home agents (HAs) whenever the MNs change their point of attachment (PoA: access point (AP) or base station (BS)) in different subnets. Thus, such registrations cause excessive signaling overhead and long service delay. To solve this problem, proxy mobile IPv6 (PMIPv6) has been proposed by the IETF NETLMM working group. In PMIPv6, a new entity called mobile access gateway (MAG) performs the mobility‐related signaling with the local mobility anchor (LMA) on behalf of the MN and establishes a tunnel with the LMA. However, a number of MNs must be associated with an MAG, which means that the MAG can be easily overloaded. Therefore, in this paper, we propose a load balancing mechanism among the MAGs in the PMIPv6 network. The PMIPv6 handover signaling procedure is extended to support the proposed load balancing mechanism. We also discuss using IEEE 802.21 Media Independent Handover (MIH) protocol for load balancing to determine the load status at the candidate PoAs, in addition to the load status at the candidate MAGs. To evaluate the performance, we analyze the average waiting time in the queue at the MAG. Through simulations and numerical analysis, we show that the proposed load balancing mechanism can produce less queueing delay at the MAG and a higher data transmission rate at the PoA than when a load balancing operation is not performed in the PMIPv6 network. Copyright © 2009 John Wiley & Sons, Ltd.     

Multihop sensor network design for wide-band communications

This paper presents a master/slave cellular-based mobile ad hoc network architecture for multihop multimedia communications. The proposed network is based on a new paradigm for solving the problem of cluster-based ad hoc routing when utilizing existing wireless local area network (WLAN) technologies. The network architecture is a mixture of two different types of networks: infrastructure (master-and-slave) and ad hoc. In this architecture, the participating slave nodes (SNs) in each cluster communicate with each other via their respective master nodes (MNs) in an infrastructure network. In contrast to traditional cellular networks where the base stations are fixed (e.g., interconnected via a wired backbone), in this network the MNs (e.g., base stations) are mobile; thus, interconnection is accomplished dynamically and in an ad hoc manner. For network implementation, the IEEE 802.11 WLAN has been deployed. Since there is no stationary node in this network, all the nodes in a cluster may have to move together as a group. However, in order to allow a mobile node to move to another cluster, which requires changing its point of attachment, a handoff process utilizing Mobile IP version 6 (IPv6) has been considered. For ad hoc routing between the master nodes (i.e., MNs), the Ad hoc On-demand Distance Vector (AODV) Routing protocol has been deployed. In assessing the network performance, field test trials have been carried out to measure the proposed network performance. These measurements include packet loss, delays under various test conditions such as a change of ad hoc route, handoffs, etc.     

Why does it pay to be selfish in a MANET?

Routing protocols for a mobile ad hoc network have assumed that all mobile nodes voluntarily participate in forwarding others' packets. This was a reasonable assumption because all MNs in a MANET belonged to a single authority. In the near future, however, a MANET may consist of MNs that belong to many different organizations since numerous civilian applications are expected to crop up. In this situation, some MNs may run independently and purposely decide not to forward packets so as to save their own energy. This could potentially lead to network partitioning and corresponding performance degradation. To minimize such situations in MANETs, many studies have explored the use of both the carrot and the stick approaches by having reputation-based, credit-payment, and game theory schemes. This article summarizes existing schemes, identifies their relative advantages, and projects future directions     

Associative IP paging based on hierarchical mobile IPv6

IP paging is a well‐known topic, which focuses on reducing amount of binding update (BU). Various IP paging architectures rely on multicast/broadcast to send their messages in wireless network. However, multicast/broadcast is unreliable and has limited bandwidth in power saving mode. This paper uses unicast to avoid exhaustion of the limited bandwidth and reliably page mobile nodes (MNs) by layer 2's supporting acknowledgment in nature. Furthermore, we successfully suppress the amount of BU by remembering and checking MNs' ever attached on‐link care of addresses (LCoAs) in the stack, which is maintained by least recently used (LRU) algorithm for efficiently paging while MNs moving in a localized pattern. We simulate this mechanism to obtain the optimal stack size, which can dramatically reduce the signals of location update and paging. Copyright © 2009 John Wiley & Sons, Ltd.     

A survey of multicast routing protocols for mobile Ad-Hoc networks

A Mobile Ad-hoc NETwork (MANET) is composed of Mobile Nodes (MNs) without any infrastructure. MNs selforganize to form a network over radio links. In this environment, multicast routing protocols are faced with the challenge of producing multi-hop routing under host mobility and bandwidth constraint. Multicast routing plays a significant role in MANETs. In recent years, various multicast routing protocols with distinguishing feature have been newly proposed. In order to provide a comprehensive understanding of these multicast routing protocols designed for MANETs and pave the way for the further research, a survey of the multicast routing protocols is discussed in detail in this paper. Qualitatively, based on their primary multicast routing selection principle, we show that all these protocols could be placed under one of two broad routing selection categories: multicast routing based on application independence and multicast routing based on application dependence.     

Design and Performance Study for a Mobility Management Mechanism (WMM) Using Location Cache for Wireless Mesh Networks

Wireless mesh networks (WMNs) have emerged as one of the major technologies for 4G high-speed mobile networks. In a WMN, a mesh backhaul connects the WMN with the Internet, and mesh access points (MAPs) provide wireless network access service to mobile stations (MSs). The MAPs are stationary and connected through the wireless mesh links. Due to MS mobility in WMNs, mobility management (MM) is required to efficiently and correctly route the packets to MSs. We propose an MM mechanism named Wireless mesh Mobility Management (WMM). The WMM adopts the location cache approach, where mesh backhaul and MAPs (referred to as mesh nodes (MNs)) cache the MS's location information while routing the data for the MS. The MM is exercised when MNs route the packets. We implement the WMM and conduct an analytical model and simulation experiments to investigate the performance of WMM. We compare the signaling and routing cost between WMM and other existing MM protocols. Our study shows that WMM has light signaling overhead and low implementation cost.     

Energy optimal channel attempt rate and packet size for ALOHA based underwater acoustic sensor networks

The fast mobile internet protocol version 6 (FMIPv6) was suggested as a fast handover mechanism over the mobile wireless Internets in order to reduce the handover latency of a mobile node (MN). However, FMIPv6 was originally designed to deal with single MN’s. In mobile wireless Internet, a multiple MNs may do a handover at the same time as a consequence of its movement from one network to another new one. This will therefore lead to the bandwidth waste and low handover performance. This paper intends to propose a multiple handover-based mobile node (MHB-MN) control method and an enhanced FMIPv6 mechanism in order to resolve the abovementioned problem. The proposal of such an MHB-MN method aims at having one mobile node work as a guide for a group of neighboring MNs. This means that the guide of MN prepares itself for a handover before actually taking the initial steps of the actual handover operation. Based on obtained results, it is plainly observable that by MHB-MN method, the handover initiation time, handover latency and handover control messages can be reduced compared to those of FMIPv6. Furthermore, the contention of the wireless channel for multiple MNs can be improved by the use of fewer control messages. Finally, the paper introduces an analytical model to show that by enabling the MHB-MN method and enhancing the FMIPv6 method, a multiple of nodes can perform rapid handover processes with low handover latency compared to that of the FMIPv6 technique.     

Location privacy protection of maritime mobile terminals

Mobile Edge Computing (MEC) can support various high-reliability and low-delay applications in Maritime Networks (MNs). However, security risks in computing task offloading exist. In this study, the location privacy leakage risk of Maritime Mobile Terminals (MMTs) is quantified during task offloading and relevant Location Privacy Protection (LPP) schemes of MMT are considered under two kinds of task offloading scenarios. In single-MMT and single-time offloading scenario, a dynamic cache and spatial cloaking-based LPP (DS-CLP) algorithm is proposed; and under the multi-MMTs and multi-time offloading scenario, a pseudonym and alterable silent period-based LPP (PA-SLP) strategy is proposed. Simulation results show that the DS-CLP can save the response time and communication cost compared with traditional algorithms while protecting the MMT location privacy. Meanwhile, extending the alterable silent period, increasing the number of MMTs in the maritime area or improving the pseudonym update probability can enhance the LPP effect of MMTs in PA-SLP. Furthermore, the study results can be effectively applied to MNs with poor communication environments and relatively insufficient computing resources.     

A Low Cost Route Optimization Scheme for Cluster-Based Proxy MIPv6 Protocol

Proxy Mobile IPv6 (PMIPv6) is a network based mobility protocol which has been designed to relieve the mobile nodes (MNs) from participating in the mobility process and to reduce the long handoff latency of the MIPv6 protocol. However, PMIPv6 incurs a long communication path due to the triangle routing problem, in which, all packets sent by MNs are obligated to pass through the local mobility anchor. Several solutions have been proposed to mitigate this issue. However, they still incur high signaling overhead to recover the Route Optimization (RO) status after handoff. In this paper, we propose a Cluster-Based RO (CBRO) scheme for the clustered architecture of the PMIPv6, in which, the Mobile Access Gateways (MAGs) are grouped into clusters with a distinguished Head MAG (HMAG) for each. In the proposed CBRO, the RO process is relied on the HMAGs to reduce the handoff latency while achieving a fast recovery of the optimized path after handoff. The proposed CBRO is evaluated analytically and compared with the basic PMIP and the current RO schemes. The obtained numerical results have shown that the proposed CBRO outperforms all other schemes in terms of signaling cost required to recover the RO status after handoff and the total cost performance metrics.