雌激素拮抗剂ICI-182780对ZDY101调节 CHOm2细胞M2受体密度的影响

CHOm2细胞加雌二醇、ZDY101或等体积溶媒DMSO培养后通过3H-NMS结合反应测定M受体密度,并与同时加雌激素受体拮抗剂ICI-182780的细胞作对比.结果显示雌二醇组M受体密度为(123.7±23.9)fmol@mg-1,加ICI-182780后为(84.8±19.8)fmol@mg-1;相应的DMSO对照组为(89.6±22.9)fmol@mg-1,加ICI后为(66.9±22.5)fmol@mg-1;雌二醇组加ICI-182780后M受体密度降低的幅度远大于DMSO对照组.ZDY101组M受体密度为(147.3±36.9)fmol@mg-1,加ICI-182780后为(120.8±40.4)fmol@mg-1;相应的DMSO对照组为(95.9±24.0)fmol@mg-1,加ICI-182780后为(77.0±22.3)fmol@mg-1.表明雌激素和ZDY101都具有的提高M受体密度的功效,雌激素的药效可被ICI抑制,而ZDYl01的药效不被ICI抑制,说明ZDYl01有不同于雌激素的作用机制.     

调理心肾中药对拟痴呆大鼠脑组织中M和GABA受体功能的影响

以~3H-QNB和~3H-GABA为放射性配基,用受体放射性配基结合分析法,测出拟痴呆模型大鼠大脑皮层和海马组织中的M受体和小脑组织中的GABA受体的R_t值明显降低;M受体的K_D值在大脑皮层中明显减少,在海马中略有升高。小脑中GABA受体的K_D值显著降低。调理心肾中药及喜德镇(Hydergin,为国外常用抗老年痴呆药)能使降低的M受体和GABA受体的R_t值均明显升高,接近正常水平。对K_D值则有不同程度的调整作用。     

ZMS对老年大鼠脑M受体与G-蛋白偶联活性的影响

以非ＧＴＰａｓｅ水解性的［３５Ｓ］ＧＴＰγＳ在激动剂Ｃａｒｂａｃｈｏｌ作用下和Ｇ－蛋白不可逆结合,检测大鼠脑Ｍ受体与Ｇ－蛋白偶联活性,同时观察不同剂量ＺＭＳ对其活性和学习记忆的影响。结果老年组Ｇ－蛋白活性低于青年组,中、大剂量组优于老年组,并能改善学习记忆能力。Ｔａｃｒｉｎｅ无明显作用。提示老年大鼠Ｍ受体与Ｇ－蛋白偶联可能有障碍,ＺＭＳ对大鼠学习、记忆的促进作用可能与提高Ｍ受体与Ｇ－蛋白偶联活性有     

极低频电磁场暴露对大鼠学习记忆功能影响的研究

为了模拟职业工作场所极低频电磁场暴露对大鼠学习记忆和海马神经元的影响,设计50 Hz电磁场暴露系统,将实验组1(电场强度5 kV/m,磁感应强度78μT,50 Hz)和实验组2(电场强度5 kV/m,磁感应强度100μT,50 Hz)大鼠各20只,每天暴露8 h,每周5 d,持续8周。进行大鼠的学习记忆神经行为与海马脑区组织病理形态学变化研究,海马神经元细胞凋亡情况检测。结果表明,一定强度和时间的极低频电磁场暴露,具有明显的记忆功能损伤效应,对照组大鼠学习记忆能力明显强于实验组(p<0.05),神经行为功能损伤早于组织病理损伤和神经元细胞损伤,且磁场的影响较大。     

微波暴露对大鼠GLP-1及其受体表达的影响及与学习记忆功能损伤的关系

探讨了微波暴露对胰高血糖素样肽-1(Glucagon-like peptide-1,GLP-1)及其受体的影响,揭示了GLP-1及其受体变化与大鼠学习记忆功能损伤的关系.分别用Morris水迷宫评价大鼠学习记忆能力,酶联免疫吸附实验(Enzyme-linked immunosorbent assay,ELISA)检测血浆中GLP-1浓度,逆转录多聚酶链反应(Reverse transfer polymerase chain reaction,RT-PCR)检测海马组织中GLP-1R mRNA表达,原位末端标记法(Terminal deoxynucleotidyl transferase mediated Biotin-dUTP nick-end labeling,TUNEL)法检测细胞凋亡.研究发现微波暴露可导致大鼠学习记忆能力下降,血浆中GLP-1浓度降低,海马组织GLP-1R mRNA表达下降,海马脑区神经细胞凋亡.暴露前给予GLP-1可改善微波暴露所致的学习记忆功能损伤,保护神经细胞,降低海马神经细胞凋亡率.GLP-1及其受体参与了微波暴露所致的学习记忆功能损伤,证实GLP-1具有神经保护作用,并拮抗微波暴露所致学习记忆功能损伤.     

β-内啡肽、促肾上腺皮质激素、5-羟色胺和去甲肾上腺素对疼痛和针刺镇痛的影响

本工作采用化学毁损弓状核及摘除大鼠垂体、荧光分析及放射免疫测定等方法,观察到:(1)毁损弓状核后脑内β-内啡肽含量降低67%,针刺镇痛效应明显下降:将此种处理大鼠的垂体切除后,针效几乎消失,同时,电针后脑去甲肾上腺素含量明显高于电针对照组。(2)促肾上腺皮质激素不仅提高基础痛阈,增强针刺镇痛效应和吗啡镇痛效应,同时也加强中枢5-羟色胺能神经元系统的功能。(3)大鼠摘除垂体后,针效明显减弱,脑内5-羟色胺及其转换率均下降,补充ACTH后,针效基本恢复,脑内5-羟色胺含量及其转换率也显著上升。     

β-内啡肽、促肾上腺皮质激素、5-羟色胺和去甲肾上腺素对疼痛和针刺镇痛的影响

本工作采用化学毁损弓状核及摘除大鼠垂体、荧光分析及放射免疫测定等方法,观察到: (1)毁损弓状核后脑内β-内啡肽含量降低67％,针刺镇痛效应明显下降:将此种处理大鼠的垂体切除后,针效几乎消失,同时,电针后脑去甲肾上腺素含量明显高于电针对照组。 (2)促肾上腺皮质激素不仅提高基础痛阈,增强针刺镇痛效应和吗啡镇痛效应,同时也加强中枢5-羟色胺能神经元系统的功能。 (3)大鼠摘除垂体后,针效明显减弱,脑内5-羟色胺及其转换率均下降,补充ACTH后,针效基本恢复,脑内5-羟色胺含量及其转换率也显著上升。     

pEgr-IFNγ基因-放射治疗对荷瘤小鼠抑瘤效应的研究

探讨pEgr-IFNγ基因-放射治疗小鼠体内抗肿瘤作用的适宜照射剂量和质粒注入量。观察不同剂量X射线照射及瘤内不同量pEgr-IFNγ重组质粒注入,基因-放射治疗在接种B16黑色素瘤小鼠体内抑瘤效应的差异,并用酶联免疫吸附法(ELISA)法检测各组小鼠外周血IFNγ含量。结果表明,质粒注入后接受20Gy X射线照射组照后3—27d,肿瘤生长速率明显低于2Gy和10Gy照射组(p<0.001),且小鼠平均生存时间明显延长(p<0.001)。质粒注入量为20μg或30μg的联合治疗组照后3—27d,肿瘤生长速率明显低于注入量为10μg的联合治疗组(p<0.05—0.001),且平均生存时间明显延长(p<0.01)。基因-放射联合治疗组受照射后第1天和第3天外周血IFNγ含量明显高于单纯基因治疗组均为(p<0.05)和对照组,分别为(p<0.01,p<0.05)。结果显示,pEgr-IFNγ基因-放射治疗小鼠体内抗肿瘤作用的适宜照射剂量为20Gy,质粒注入量为20μg;联合治疗组可能通过诱导瘤内IFNγ基因表达增强,使血液中的IFNγ浓度升高,进而增强机体免疫功能和抗肿瘤能力。     

HL-1M多脉冲分子束注入的CCD摄像

HL-1M装置实现了一种新的气体加料改善约束的方法——分子束注入(用多脉冲高速分子束注入加料)。多脉冲分子束注入加料可以控制等离子体密度分布、改善约束性能和提高密度极限。HL-1M托卡马克的多脉冲分子束注入得到了高的加料效率、改进了能量约束并维持了较高的密度峰化截面。分子束加料τ_E的改善和     

HL-1M弹丸注入实验中等离子体的约束改善特征和中心磁流体动力学特性研究

在HL-1M实验中,多发弹丸注入到欧姆加热放电中,明显改善了等离子体约束特性。与相同条件下的欧姆加热放电相比,能量约束时间提高了约30％。等离子体约束的改善是因为弹丸注入后在等离子体中心区域形成了高度峰化的密度和压强分布。实验发现弹丸穿透的深度决定了密度和压强分布的峰化程度,而等离子体中心的磁流体力学活性义随着     

Stopping powers of Be,Al, Ti,V, Fe,Co, Ni,Cu, Zn,Mo, Rh,Ag, Sn,Ta, Pt and Au For 6.5 MeV protons

The stopping powers of Be, Al, Ti, V, Fe, Co, Ni, Cu, Zn, Mo, Rh, Ag, Sn, Ta, Pt and Au for 6.5 MeV have been measured using a surface barrier silicon detector. The accuracy of the results is ± 0.3%. The detail of the experimental procedures is described. The results have been compared with the Risø data and the Aarhus data of Andersen et al. The present results for Al, Cu and Ag agree very well with the Aarhus results at 6.5 MeV. The mean excitation energy has been extracted from the present results using the Bonderup shell correction, the Barkas correction and the Bloch correction. The parameters x and b which appear in the Bonderup shell correction and the Barkas correction have been newly determined as x = 1.358 and b = 1.3. The present mean excitation energy agrees very well with the I values obtained by other authors. It has been confirmed that the Bonderup shell correction is quantitatively correct for 6.5 MeV protons.     

Discovery of dysprosium,holmium, erbium,thulium, and ytterbium isotopes

Currently, thirty-one dysprosium, thirty-two holmium, thirty-two erbium, thirty-three thulium, and thirty-one ytterbium isotopes have been observed and the discovery of these isotopes is described here. For each isotope a brief synopsis of the first refereed publication, including the production and identification method, is presented.     

Discovery of yttrium, zirconium, niobium, technetium, and ruthenium isotopes

Currently, thirty-four yttrium, thirty-five zirconium, thirty-four niobium, thirty-five technetium, and thirty-eight ruthenium isotopes have been observed and the discovery of these isotopes is described here. For each isotope a brief synopsis of the first refereed publication, including the production and identification method, is presented.     

Gamma-Ray Production Cross Sections for Interactions of 14.8 MeV Neutrons with O,Na, Al,Cl, Cr,Fe, Ni,Cu and Pb

Production cross sections for 85 discrete γ-rays at 125° were measured with a Ge(Li) detector for interactions of 14.8 MeV neutrons with natural samples of O, Na, Al, Cl, Cr, Fe, Ni, Cu and Pb. The obtained cross sections were compared with the results of previous works. For O, Na, Al, Cr and Ni, the present results agree with the previous data measured with monoenergetic neutron sources; for Cl, Fe, Cu and Pb, the present results are larger than the previous data. In comparison between the present results shown by histograms of γ-ray energy and unfolded data, a considerable discrepancy is found from some of the previous data for Fe.     

Determination of Microamount of Aluminum,Chromium, Copper,Iron, Manganese,Molybdenum and Nickel in Pure Water by Extraction Photometry

Aluminum, iron (III), copper, and molybdenum 8-quinolinol complexes, chromium (III) and manganese 8-hydroxyquinaldine complexes and nickel dimethylglyoxime complex can be quantitatively extracted into chloroform from large volumes of aqueous phases adjusted to an approriate pH for each metal. These metals can be determined by measuring the absorbance of the organic phases containing their complexes at an appropriate wavelength for each metal. By the proposed method, quantities exceeding 3 p.p.b. of aluminum, chromium, and manganese, and 5 p.p.b. of copper, iron, molybdenum and nickel can be determined when a 500 ml sample of water is available.