Use of microbial transglutaminase and nonmeat proteins to improve functional properties of low NaCl, phosphate-free patties made from channel catfish (Ictalurus punctatus) belly flap meat

ABSTRACT:  This study was aimed at developing value-added low sodium chloride (NaCl), phosphate-free restructured patties using minced channel catfish ( Ictalurus punctatus ) belly flap meat. The effect of microbial transglutaminase (MTGase) and nonmeat proteins (isolated soy protein, ISP, and whey protein concentrate, WPC; 1.7%, respectively) alone and in combination were evaluated to improve cooking yield and textural properties in patties with reduced NaCl and no phosphate. The concentration effect of MTGase (0.05% to 0.7%) was also studied. The addition of MTGase increased textural properties such as binding strength, hardness, cohesiveness, chewiness, and springiness, but decreased cooking yield of the patties ( P < 0.05). Isolated soy protein increased cooking yield ( P < 0.05), but did not affect textural properties. Inclusion of WPC did not increase cooking yield or impact textural properties of patties. The combination of MTGase and ISP significantly increased both the cooking yield and textural properties of patties. As the concentration of MTGase increased at constant ISP, the textural properties of cooked patties significantly increased, but cooking yield decreased ( P < 0.05). In conclusion, we suggest that the combination of 0.05% to 0.1% of MTGase with 1.7% ISP is optimal for development of a low NaCl, phosphate-free patty using minced catfish belly flap meat.     

Effect of microbial transglutaminase, sodium caseinate and non-fat dry milk on quality of salt-free, low fat turkey döner kebab

Effects of microbial transglutaminase (MTGase) and its combinations with sodium caseinate (SC) or non-fat dry milk (NFDM) on quality of salt-free, low fat turkey döner kebab were investigated. The purpose of this study was to prevent quality deteriorations (e.g. cooking loss, textural problems) caused by a lack of salt in the product. The results of this study indicated that the use of MTGase can minimize textural quality loss caused by a lack of salt in turkey döner manufacture (p < 0.05). When the enzyme was combined with SC, or NFDM, its effect on texture was enhanced (p < 0.05) and improved cooking yield, moisture and sensory properties compared to the corresponding control manufactured without the enzyme, salt, SC and NFDM. (p < 0.05), however, there was no significant effect on pH and color As a result, the possible quality problems which may occur in salt free, low fat turkey döner can be overcome by the use of combinations of MTGase with SC or NFDM.     

Prediction of sensory textural properties from rheological analysis for process cheeses varying in emulsifying salt,protein and moisture contents

Textural characteristics of process cheeses varying in emulsifying salt (disodium phosphate), protein and moisture contents were evaluated by rheological compression using texture profile analysis and by sensory evaluation. The primary objective of this study was to predict sensory textural parameters using instrumental rheological parameters. All sensory parameters correlated with one or more instrumental parameters, e.g. rheological firmness versus sensory firmness (R = 0.98, P < 0.001), rheological chewiness versus sensory rubbery (R = 0.92, P < 0.001) and rheological chewiness versus sensory chewy (R = 0.86, P < 0.001). Partial least squares calibration models were developed for each of nine sensory parameters using instrumental parameters. Principal component analysis of instrumental and sensory parameters illustrated relationships among parameters. It was shown that instrumental parameters could be used to supplement sensory evaluation of process cheese texture. Increasing emulsifying salt content increased firmness, springiness and chewiness and decreased adhesiveness, mouthcoating and mass formation. Increasing protein content resulted in increased fracture strain and stress and chewiness and decreased melting. Increasing moisture content increased cohesiveness and decreased firmness and chewiness. Copyright © 2007 Society of Chemical Industry     

Physicochemical,textural and sensory properties of white soft cheese made from buffalo and cow milk mixtures

The physicochemical, textural and sensory properties of white soft cheeses made from three different buffalo and cow milk mixtures (100:0, 70:30 and 30:70) during 3‐month storage were studied. The increase in buffalo milk concentration resulted in increasing total cheese yield, dry matter (DM) and fat retention and fat in DM content. However, it caused reductions in moisture content, salt intake, hardness, chewiness, elasticity, sensory hardness and sensory cohesiveness of the samples. The percentage of water‐soluble nitrogen to total nitrogen increased during storage resulting in decreased fracturability, hardness (textural and sensory), cohesiveness (textural and sensory), springiness, chewiness and elasticity. The panellists evaluated the white soft cheese made with buffalo milk as the most acceptable.     

Combination treatment of high-pressure and CaCl2 for the reduction of sodium content in chicken meat batters: effects on physicochemical properties and sensory characteristics

A combination of high-pressure processing (HPP, 200 MPa, 10 min) and CaCl₂ (0.2%, w/w) on the cooking loss (CL), expressible moisture (EM), textural properties (hardness, springiness, cohesiveness, chewiness and firmness) and sensory attributes of reduced-sodium (1.25% NaCl) chicken meat batters (RCMB) was investigated. The results revealed that combining HPP with CaCl₂ significantly decreased EM, elevated sensory properties and acted synergistically in reducing CL and improving hardness, chewiness and firmness, which enabled RCMB to obtain quality characteristics similar to those with high salt (2.5% NaCl) content. These changes brought about by combined HPP and CaCl₂ could be attributed to increased apparent viscosity and proportion of immobilised water, enhanced rheological (elastic) properties and a denser, homogeneous protein gel network with evenly dispersed small fat globules. It is interesting to take advantage of the synergy between moderate HPP and low concentrations of CaCl₂ to develop meat products with reduced sodium content.     

Chemical, Textural and Sensory Characteristics of Precooked, Restructured Lamb Shoulder Roasts as Influenced by Grinding Method, Salt Level and Vacuum Massage Time

The influence of level of grind (kidney plate only vs kidney plate plus coarse grinding 25% of raw meat weight), salt level (0.5 vs 1.0%) and vacuum massage time (1.0 vs 1.5 hr) on the chemical, textural and sensory characteristics of precooked, restructured lamb shoulder roasts were studied. Grinding method of kidney plate only decreased the fat content of the cooked product, while the higher salt level (SL) resulted in roasts with higher moisture and lower protein. Calories were reduced by 5% when 1.0% SL roasts were vacuum-massaged for 1.5 vs 1.0 hr. Increasing level of grind and SL decreased cooking losses and increased objective chewiness. Flavor differences were detected by sensory analysis due to the addition of coarse ground trimmings to lean ground through a kidney plate only.     

面条水煮工艺条件的研究

通过调节烹调用水的pH 值、离子强度,考察二者对煮制面条的感官性状、出品率、溶出率的影响,以确定烹调用水中最佳食醋和食盐的添加量。分析面条质构指标、感官指标及面条出品率和溶出率间的相关性,同时对该优化水煮制的面条和普通饮用水煮制的面条做了质构分析。结果表明：面条质构指标、感官指标及面条出品率和溶出率间存在着显著的相关性,不同的水煮条件会对面条品质产生影响,当烹调用水中9°食醋添加量为1.2%(V/V)食盐添加量为0.3g/100L 时,面条的感官性状、黏附性及弹性得到改善。     

Microstructural,cooking and textural characteristics of potato (Solanum tuberosum L) tubers in relation to physicochemical and functional properties of their flours

Potato tubers from six different cultivars were freeze‐dried, ground into flour and analyzed for thermal, pasting and textural characteristics (using differential scanning calorimetry, Rapid Visco analyzer and Instron universal testing machine, respectively) to study the relationship between flour characteristics and cooked potato mealiness. The potatoes with higher sensory mealiness scores resulted in flours having lower transition and pasting temperatures, higher amylose content, setback, peak and final viscosity. The flour gels from the mealier potatoes also exhibited higher values of textural parameters such as hardness, cohesiveness, chewiness and springiness. The microstructure of the tuber parenchyma (studied using scanning electron microscopy), cooking and sensory characteristics of potatoes were found to be related to the pasting and textural characteristics of their flours. Potato cultivars with lower mealiness scores, loosely packed cell arrangement, with comparatively large‐size cells and thinner cell walls showed lower values of textural parameters for both raw and cooked potatoes. This information may prove useful for the selection of potato cultivars with desirable textural and flour‐making properties for specific end‐uses. Copyright © 2005 Society of Chemical Industry     

Effect of Modified Wheat Gluten on Boiling Resistance Capacity of Pork Meatballs

The effect of the modified wheat gluten (MWG) extender, prepared by alcalase‐based hydrolysis and transglutaminase cross‐linking, on meatballs was analyzed in this study. Here, we studied the effect of MWG addition on the boiling resistance capacity of pork meatballs (MB‐MWG) at high temperature (100 °C) and increasing cooking time; meatballs with added soy protein isolates (MB‐SPI) and raw wheat gluten (MB‐WG) were used as references. The cooking loss, water‐holding capacity (WHC), and textural properties of meatballs were investigated. The results revealed that MB‐MWG showed lower cooking loss, which decreased by 49.16% compared to meatballs without added extenders when treated for 30 min. The WHC of MB‐MWG significantly increased from 80.68% to 95.42%. The hardness, springiness, and chewiness (textural properties) of MB‐MWG were also significantly increased by 97.05%, 6.68%, and 121.96%, respectively. The addition of MWG increased the cross‐linking in meatballs during the cooking process, as indicated by the higher G′. SDS‐PAGE indicated an obvious decrease in myosin heavy chain in MB‐MWG cooked for 30 min at 100 °C, which was attributed to the interaction of myofibrillar proteins in pork meat with MWG. The nuclear magnetic resonance T₂ relaxation time patterns indicated that MWG addition caused an increase in the bound water content, and decrease in the free water content, of meatballs. An analysis of the microstructures revealed that the MB‐MWG formed the most regular and compact network. Therefore, MWG could be used as an ingredient to facilitate the processing of meat products.     

Effect of the combination of various NaCl levels and soy protein isolate on the quality characteristics of smoked pork loins

The objectives of this study were to evaluate the physicochemical, textural and sensory properties of smoked pork loins (PL) containing various levels of NaCl (0.0–2.0%) alone or in combination with soy protein isolates (SPI; 1.0%). The pH values, moisture, fat, and protein contents (%) of smoked PL were 5.97–6.17, 63.3–69.1%, 2.64–4.26%, and 20.2–26.6%, respectively. Increased NaCl levels increased moisture contents (%) and sensory scores, as well as reduced Hunter redness and yellowness and cooking loss (CL, %) (P < 0.05). A NaCl level of 1.0% had resulted in a CL (%) level similar to those at regular‐salt levels (1.5% and 2.0% NaCl). When 1.0% SPI was incorporated with smoked PL, the NaCl level of 0.5% was also similar to the CL (%) at higher NaCl levels and improved sensory scores. Thus, a NaCl level of at least 1.0% was required for the manufacture of smoked PL; however, smoked PL could be manufactured with 0.5% salt when 1.0% SPI was included as part of the manufacture of smoked PL to effect quality characteristics similar to those achieved with regular‐salt (1.5% and 2.0% NaCl) smoked PL.     

Use of dairy proteins and microbial transglutaminase to obtain low-salt fish products from filleting waste from silver carp (Hypophthalmichthys molitrix)

Restructured fish products from the filleting waste from silver carp (Hypophthalmichthys molitrix) were obtained using sodium caseinate (1%), whey protein concentrate (WPC) (1%) or MTGase (0.3%) at different levels of salt (0%, 1% or 2%). The restructured products were obtained by incubating at 40 °C for 60 min and then at 90 °C for 20 min. Changes in the mechanical properties were studied by measuring changes in the textural profile analyses, punch test and torsional test analyses and expressible water. The mechanical properties increased when the salt level was also increased. The mechanical properties of non-salted and low-salt products were increased by using dairy proteins. Sodium caseinate had a greater effect in improving mechanical properties than WPC. MTGase increased expressible water. The results obtained showed that the mechanical properties of low-salt restructured fish products can be improved by using dairy proteins combined with MTGase, with a slight increase in expressible water.     

Negative effect of combining microbial transglutaminase with low methoxyl pectins on the mechanical properties and colour attributes of fish gels

The objective of this work was to determine the effect of combining different concentrations of microbial transglutaminase (MTGase) and types of low methoxyl (LM) pectin on the mechanical properties (textural profile analysis, puncture test) and the colour attributes of fish restructured products. A disruptive effect was observed when LM pectin and MTGase were added to fish gels. Adding 1% MTGase with LM32 pectin significantly decreased the hardness (3.85 kg), springiness (0.631) and chewiness (0.434 kg). When LM32 pectin and 0.1% MTGase were added together, significant decreases of the three puncture test parameters were observed. The chroma of fish gels decreased significantly when 0.3% MTGase was added. Fish gels containing the LM35 pectin had higher chroma values than all other fish gels with the same level of MTGase. Hue value was increased in all fish gels after increasing the MTGase level. The results obtained showed that LM pectin is not suitable for use in products containing MTGase.     

不同种木薯变性淀粉对鸡肉肠品质的影响

对比研究了在斩拌过程中添加6%的木薯变性淀粉(醋酸酯淀粉、交酯淀粉和磷酸酯淀粉)和木薯原淀粉对鸡肉肠品质的影响。结果发现:添加变性淀粉的鸡肉肠的产率差异不显著(P>0.05);添加醋酸酯淀粉的鸡肉肠的L*值最大;添加变性淀粉的产品的质构特性要明显好于添加原淀粉的鸡肉肠(P<0.05),其中添加交酯淀粉的产品的硬度、咀嚼性、弹性和凝聚性最高。感官评定结果显示,添加醋酸酯淀粉产品的品质优于添加其它种类变性淀粉的产品。醋酸酯木薯淀粉更适合于肉类产品实际生产中。     

Particle Size and Mixing Time Effects on Sensory and Physical Properties of Low-fat, High-moisture Pork Frankfurters

Changes in particle size affected measured characteristics more than changes in mixing time. Sensory results indicated that use of a 2.0 mm plate produced the coarsest (P<0.05) product. CIE b* values decreased (<0.05) as particle size was increased. Products produced with a 2.0 mm plate compared to a 1.4 mm plate had higher (P<0.05) hardness values and total energy to shear. Particle size did not affect (P>0.05) cooking loss, springiness, cohesiveness, or chewiness. Additional mixing tended to decrease sensory particle size and increase springiness and chewiness.     

Effect of mungbean [Vigna radiata (L.) Wilczek] protein isolates on the microbial transglutaminase‐mediated porcine myofibrillar protein gels at various salt concentrations

This study was performed to investigate the effects of mungbean protein isolates (MPI) as a meat/water binder on the MTGase‐mediated porcine myofibrillar protein (MP) gels at 0.15, 0.3, and 0.45 m salt concentrations. The general property of MP gel was evaluated by pH, cooking loss (CL) (%) and gel strength (gf). Protein–protein interactions among MPI, MTGase, and MP during cooking were also assessed using gel electrophoresis, thermal analysis and microstructure. When salt content was reduced, gel CL (%; P < 0.05) was increased while pH and gel strength (gf) values were decreased (P < 0.05). Addition of MTGase to MP increased pH, CL (%), and gel strength (gf) values, while co‐addition of MTGase and MPI induced synergistic effects on the MP gel strength (gf; ≥0.3 m salt concentration; P < 0.05). In scanning electron micrograph images, increase of salt concentrations made MP gels more swollen and interwoven or conglomerated, regardless of treatment. In conclusion, addition of MPI and MTGase strengthened gel‐forming ability and improved cooking yield of MP gel at salt concentration (≥0.3 m ).     

潮汕脆性肉丸的感官评定与质构评价相关性分析

目的:探索一种综合评价潮汕脆肉丸脆性品质的方法。方法:采用质构剖面法（texture profile analysis,TPA）和穿刺实验测定肉丸的质构特性,同时进行感官评定实验,并对感官和质构指标之间进行相关性分析。结果:TPA实验的硬度、咀嚼性与感官评定指标硬度、弹性、脆性、咀嚼性之间存在显著相关性（r=0.554~0.793,P<0.05）,TPA实验的回复性与感官评定的脆性、组织状态之间存在显著相关性（r=0.556,0.625,P<0.05）。穿刺实验的破裂力与感官评定指标之间均存在显著相关性（r=0.595~0.709,P<0.05）。运用主成分分析从仪器质构指标数据中提取出2个最主要的独立成分,这2个主成分的方差贡献率累计达74.5%。结论:潮汕肉丸的脆性可以采用TPA实验的硬度、咀嚼性和穿刺破裂力的定量评价结合感官评定进行综合评价,为构建客观、精确、便捷的潮汕脆性肉丸感官性状评价体系奠定基础。     

Response surface methodology study on the effects of salt,microbial transglutaminase and heating temperature on pork batter gel properties

Response surface methodology was used to investigate the main effects and interactions of sodium chloride (0–2%), microbial transglutaminase preparate (MTG, 0–0.6%) and heating temperature (HT, 70–100°C) on water binding, textural and colour characteristics of pork batter gels cooked to an internal temperature of 70 °C. Lower salt gels showed decreases in hardness, chewiness and elastic properties, as well as significant reduction in the cooking yield and increase of expressible moisture. Salt levels also affected gel colour parameters, with Hunterlab a* and b* values being inversely correlated with salt concentration. MTG addition favourably reduced the cooking loss and increased hardness and chewiness of gels, but was not able to improve these parameters in low-salt products to the same levels as the high-salt products. Heating temperature was found to have relatively minor effect, primarily through its interaction with salt level and in a quadratic term affecting the elasticity and springiness of the gels.     

Effects of high pressure level and holding time on properties of duck muscle gels containing 1% curdlan

The effect of high pressure processing (HPP) on properties of duck muscle gels (DMG) containing 1% curdlan was investigated. The application of > 300 MPa could result in the decrease of cooking loss of DMG in water binding capacity, the increase of L value and the decrease of a value and b value in color, the increase of hardness, springiness, cohesiveness and chewiness in textural parameters (P < 0.05), while the pressure-holding time had no obvious influence. Those experimental results could be attributed to the interactions among protein molecules and the interactions between protein molecules and curdlan molecules created or enhanced by HPP. Overall, the use of curdlan instead of fat and the application of HPP may provide a novel approach to achieve low-fat (< 6% fat) and low-salt (1% salt) DMG products with good properties and high yields.

Industrial relevance

To provide healthier meat foods like low-fat (< 6% fat) and low-salt (about 1% salt) duck meat products, the application of HPP might be of great interest for industrial manufacture and can yield the products bearing high water binding capacity and good textural properties.     

The use of selected hydrocolloids to enhance cooking quality and hardness of zero‐salt noodles

The effects of table salt (TS) and hydrocolloids on water‐holding capacity, optimum cooking time, cooking qualities, pH and textural properties of noodles were investigated. TS, xanthan gum (XG), carrageenan (CRG), Arabic gum (AG) and locust bean gum (LBG) were added at 0.5%, 1.0%, 1.5% and 2.0% of flour weight. XG, CRG and LBG contributed to significantly (P < 0.05) higher water‐holding capacity of dough and firmer texture, but significantly (P < 0.05) lower cooking loss than zero‐salt noodles (ZSNs) and white‐salted noodles (WSNs). Hydrocolloids contributed in shorter optimum cooking time than ZSNs. Springiness of noodles was not significantly (P > 0.05) affected by increment of TS and hydrocolloids. TS1.5, ZSN‐XG1.5, ZSN‐XG2.0 and ZSN‐CRG1.5 had significantly (P < 0.05) higher cooking yield than ZSNs. The increment of TS and hydrocolloids had significantly (P < 0.05) increased the pH values of noodles. ZSN‐XG2.0, ZSN‐CRG1.5 and ZSN‐LBG1.5 may be useful to enhance ZSNs due to the better noodles qualities than ZSNs.