The influence of transglutaminase treatment on functional properties of set yoghurt

The objective of this study was to investigate the effect of transglutaminase (Tgase) on the functional properties of set yoghurt, due to enzymatic cross-linking of the milk proteins. Yoghurt samples were prepared from three formulations: reconstituted skim milk, and whole milk containing two different solid-non-fat (SNF) content, with four enzyme treatments: Control (no enzyme) and three microbial Tgase treatments: Pre-incubated 90 min, Pre-incubated and inactivated after 90 min, Not pre-incubated. The modifications which were caused by Tgase were found to be more clear when Tgase was active in set type yoghurts. It was found that nonfat yoghurt sample with lower SNF content can be produced with improved textural properties using Tgase.     

Effect of addition of gelatin on microstructure of acidic milk gels and yoghurt and on their rheological properties

A study was made of the effect of the addition of gelatin on the microstructure of acid-heat-induced milk gels (90°C, pH=5.3) and yoghurt with and without the addition of 5% of milk solids, and a comparison was made with the microstructure of acidic milk gelatin gels obtained without heating (pH=5.3). It was seen that in the acid-heat-induced gels and in yoghurt the gelatin interacted with the network of milk proteins as a connection between the clusters formed, whereas it was the gelatin alone that was the basis of the formation of the gel when the milk did not reach the casein coagulation point (pH=5.3, unheated). The results of firmness tests indicated that the addition of 1.5% of gelatin developed fairly firm, deformable systems in all the cases studied, with a definite break point and almost total absence of syneresis. Dynamic rheology showed that the yoghurts with added gelatin exhibited more solid-like behaviour than the ones prepared without it.     

Effect of storage time on sensory and instrumental properties of skim‐milk yoghurt obtained with microbial transglutaminase

Not many studies have examined changes in microbial transglutaminase (MTG) yoghurts during storage. In this work, yoghurts manufactured with skimmed milk and treated with MTG were evaluated in refrigerated storage for 35 days. Instrumental measurements showed that firmness, consistency and pH values in MTG yoghurt experienced a significant increase with the storage time. However, sensory measurements showed that MTG yoghurts were not affected significantly by storage time. Acid taste scores only increased significantly with storage time in the control samples. Microbial transglutaminase treatment showed a positive effect on textural properties and allowed syneresis to be prevented completely during storage.     

Effects of various whey proteins on the physicochemical and textural properties of set type nonfat yoghurt

In this study, the changes during storage in the physicochemical, textural and sensory properties of nonfat yoghurts fortified with whey proteins, namely whey protein concentrates (WPC), whey protein isolates and whey protein hydrolysates, were investigated. Enrichment of nonfat yoghurt with the whey protein additives (1% w/v) had a noticeable effect on pH, titratable acidity, syneresis, water‐holding capacity, protein contents and colour values on the 14th day of storage (P < 0.01). The addition of whey proteins to the yoghurt milk led to increases in the hardness, cohesiveness and elasticity values, resulting in improved textural properties. The addition of WPC improved the texture of set‐type nonfat yoghurt with greater sizes in the gel network as well as lower syneresis and higher water holding capacity. This study suggests that the addition of whey protein additives used for fortification of yoghurt gave the best textural and sensory properties that were maintained constant during the shelf life.     

Effect of using transglutaminase on physical,chemical and sensory properties of set-type yoghurt

This study was conducted in order to evaluate the effects of transglutaminase (TGase) addition on some properties of set-type yoghurts such as titratable acidity, lactic acid, tyrosine, viscosity, gel firmness, syneresis, aroma compounds, sensory analysis, and micro-structural properties. The enzyme was added to yoghurt-milk at different production steps (after homogenization, after pasteurization and together with starter culture addition) and two different incubation times (10 min and 1 h) were used. Five TGase treated yoghurt samples and control sample were analyzed on 1st, 10th, and 20th days of storage. TGase addition did not cause significant changes on chemical properties of yoghurts. However, enzyme addition after pasteurization increased the gel strength and decreased the syneresis. Results of electron microscope showed that enzyme addition led proteins to be distributed more evenly in gel network due to the formation of cross-links between proteins.     

Using fractal image analysis to characterize microstructure of low-fat stirred yoghurt manufactured with microparticulated whey protein

Differences in the microstructure of low fat yoghurt manufactured with microparticulated whey proteins used as fat replacer were investigated. Images were obtained by confocal laser scanning microscopy and studied using a technique for image analysis that combines an initial 2D-wavelet compression followed by fractal analysis and inspection of the fractal curves by principal components analysis (PCA). One commercial and three experimental microparticulated ingredients with different chemical characteristics were used in the yoghurt formulations and compared to both full and low fat yoghurts without fat replacer. The results showed that the amount of native and soluble whey proteins present in the microparticles had a positive influence on the structure of the formed gel. The created structure, dominated by dense aggregates and low amount of serum, had an increased degree of self similarity or fractality with yoghurts in which fat was present.     

变性对转谷氨酰胺酶在乳蛋白间交联影响初探

利用SDS-PAGE电泳结合凝胶成像分析技术，比较了在非变性、加入还原剂变性和加热后再加入还原剂变性三种条件下转谷氨酰胺酶对酪蛋白和乳清蛋白之间的交联情况。结果表明：在非变性条件下，酪蛋白质量分数下降96％，乳清蛋白下降15％，酪蛋白和乳清蛋白几乎不能交联。超分子量聚合物是酪蛋白单一聚合物，α-乳白蛋白形成部分低聚体；在加入还原剂时，酪蛋白质量分数下降86％，乳清蛋白下降30％，反应4h后有少量乳清蛋白和酪蛋白中某一组分交联；预热更有助于酪蛋白和乳清蛋白聚合，在第三种条件下，反应24h后乳清蛋白下降60％。     

Development of yoghurt from ovine milk with enhanced texture and flavour properties

Six commercial starter cultures, all blends of Streptococcus thermophilus sp. and Lactobacillus bulgaricus sp., were compared for enhanced texture and flavour in yoghurt prepared from ovine milk. The fermentation patterns differed between starters, with YF‐L903 (E) showing the fastest fermentation but slower post‐acidification. YC‐X11 (A) produced the slowest acidification, while YC‐380 (B) resulted in the fastest post‐acidification. Textural and colour properties were affected significantly by the culture used. YF‐L903 (E) enhanced the firmness and luminosity of the yoghurt over storage, increasing mouthfeel and creaminess, as compared to yoghurts prepared with the other starter cultures starters studied.     

Effect of enzymatic cross-linking of milk proteins on functional properties of set-style yoghurt

This paper presents research on the effect of enzymatic cross-linking of milk proteins on the properties of yoghurt. Whole milk was incubated with transglutaminase (TG) prior to fermentation (2 h, 40°C, E/S ratio 1/2000). Enzyme action was stopped by heating (1 min, 80°C). Skim-milk was treated by simultaneous use of TG and thermophilic yoghurt starter culture without inactivation of the enzyme. A TG treatment of milk prior to fermentation led to prolonged fermentation, while the concomitant use of TG and culture had no influence on fermentation time. Post acidification of yoghurt during storage was lower for products from enzyme-treated milk. This applies both for products cross-linked prior to fermentation with enzyme inactivation, and for simultaneous use of culture and TG without inactivation of the enzyme. Scanning electron microscopic studies revealed that a TG treatment of milk led to reduced mesh sizes of the protein network, and a more regular distribution of the proteins in the yoghurt gel. As a result, yoghurt products from enzyme-treated milk showed increased gel strength and less syneresis, especially when the enzyme was not inactivated. Sensory studies revealed that odour and consistency properties of products from TG-treated milk were assessed as less 'yoghurt specific'. On the other hand, products from enzyme-treated milk were described as being more creamy, indicating that a TG treatment may simulate fat in fermented milk products.     

The quality of set‐style yoghurt responsible to partial lactose hydrolysis followed by protein cross‐linking of the skimmed milk

Skimmed milk used for set‐style yoghurt production was treated with lactase at 0.1 g/kg for 30 min to give partial lactose hydrolysis and then treated with horseradish peroxidase and glucose oxidase at 200 and 6 kU/kg protein to result in protein cross‐linking. Two treatments conferred higher apparent viscosity on the milk, but led to the yoghurt prepared from it with insignificantly different chemical compositions to the counterparts (P > 0.05). The prepared yoghurt also showed decreased syneresis (about 17.7%), higher apparent viscosity and viscoelastic modulus, firmer texture and finer microstructure. This ternary enzyme system is a potential approach to improving the quality of set‐style yoghurt.     

The effects of using transglutaminase,exopolysaccharide‐producing starter culture and milk powder on the physicochemical,sensory and texture properties of low‐fat set yoghurt

This study focused on the effects of transglutaminase (TGase), exopolysaccharide (EPS)‐producing starter culture or skim milk powder addition on some properties of low‐fat set yoghurt. The incorporation of TGase to yoghurt did not affect the acidity of the experimental samples. However, the proteolytic activity of the starter culture significantly decreased in TGase‐treated yoghurt. Addition of TGase to yoghurt with a low dry matter content (11.08%) resulted in low serum separation and gel firmness and viscosity close to those of yoghurt with a higher dry matter content (14.81%). According to the sensory results, it would be possible to produce an acceptable product with a low dry matter and low fat content using TGase. The results shown that the use of EPS‐producing starter cultures for the purpose of improving the texture properties of low dry matter content yoghurt could not be recommended.     

Microstructure, rheology and storage stability of low-fat yoghurt structured by carrot cell wall particles

Plant cell wall particles derived from fruits and vegetables are natural fibre materials with a low calorie content that can be used as a healthy alternative to gum stabilisers and starches for structuring low-fat yoghurt. In this study we investigated the effect of cell wall particle (CWP) addition on the gelation kinetics, viscoelastic properties, microstructure, texture and whey loss of the set yoghurt gels as a function of CWP concentration, particle size and storage time. Three particle sizes of dried carrot CWP (d_0.5 = 34, 71 and 80 ??m) were produced from an industrial carrot pomace. Rehydrated CWP was added to skim milk prior to acidification. The results showed that the addition of carrot CWP accelerated the rate of pH reduction and induced earlier gelation. The gel viscoelastic properties were enhanced with increased CWP concentration. This was accompanied with progressive reduction in the whey loss. The smallest cell wall particles (d_0.5 = 34 ??m) gave better gel strength and lower whey loss compared to the larger CWP particles, possibly due to higher contact between the CWP and casein particles thus contributing to the stronger gel network. The CLSM images of yoghurt gels containing CWP showed that carrot CWP occupied the void space within casein particle network. The enhanced gel strength and reduced whey loss achieved by the addition of CWP were maintained throughout the 28 day storage period. The reduction of fermentation processing by almost 1 h, yet still achieving good gel properties for the yoghurt type product could be a significant benefit from a manufacturing point of review.     

微生物转谷氨酰胺酶催化乳清蛋白聚合研究

采用SDS－PAGE分析，研究了不同条件下微生物转谷氨酰胺酶（MTGase）催化乳清蛋白（WPI）聚合。结果显示，MTGase可催化乳清蛋白的β－乳球蛋白（β-LG）和α－乳清蛋白（α－LA）聚合，形成低聚物或生物聚合物，其中β-LG更易受MTGase的催化，当TGase酶浓度一定时（0.5U/mL),TGase催化WPI聚合的最佳底物质量分数范围为2％－4％，对WPI进行加热预处理，同时添加还原剂，可明显提高MTGase对WPI的催化活性，MTGase催化WIP的最适PH值范围为6．5－7．5，当WPI经预热处理（85℃，15min），同时添加20mmol/L的DTT，TGase催化WPI聚合12h，可使质量分数为92％的β-LG和质量分数为75％的α－LA聚合。     

Rheological and sensory properties of stirred yoghurt with inulin‐type fructans

This study concerns physicochemical and sensory analysis of stirred yoghurts, in which skim milk powder was partly replaced by different inulins. The composition of basic solutions contained 12% skim milk powder and 4% inulin, while reference yoghurts contained 16% SMP or whole milk powder. Rheological and sensory analysis showed that one‐quarter of milk powder could be substituted by long‐chain inulin in low‐fat stirred yoghurt. Short‐chain inulin and oligofructose can potentially be applied in the production of drinking yoghurt. Sensory evaluation showed that milk fat cannot be successfully substituted by inulin.     

Sensory evaluation of ovine milk yoghurt with inulin addition

The purpose of this study was to elaborate prebiotic yoghurt using ovine milk and different inulin concentrations (0, 2 and 6%). Samples were submitted to laboratory analysis and two sensory tests with 100 consumers. The results of apparent viscosity and firmness determined by instrumental analysis of each yoghurt and control differed significantly. Just‐About‐Right showed consistency as the main attribute that varied in treatments. According to penalty analysis, prebiotic yoghurt containing 6% inulin had minor penalties, which indicates its advantage in the quest to better consumer demands. Hence, this treatment demonstrated better results as well as the many prebiotic benefits associated with an adequate fibre amount.     

Effect of microbial transglutaminase on the rheological and textural characteristics of black soybean packed tofu coagulating with Agar

The objective of this study was to investigate the effects of microbial transglutaminase (MTGase) on the rheological and textural characteristics of black soybean packed tofu containing agar as the coagulant. Results showed that the addition of MTGase increased the gelation temperature of soymilk, and produced a firmer and more elastic packed tofu with low cooking loss. Dynamic rheological and textural parameters of the tofu were significantly affected by enzyme concentration, incubation temperature and time. Both G′ and η∗ were positively correlated to the hardness, gumminess and adhesiveness of packed tofu, thus the rheological parameters (G′ and η∗) were good indicators of the texture of the packed tofu. Results suggested that good quality packed tofu could be produced by mixing 90 g black soymilk solid and 2 g agar powder in 1 L of water and incubating with 10 g MTGase for 30 min at 55 °C.     

The effect of transglutaminase on the properties of milk gels and processed cheese

The aim of this study was to investigate the influence of transglutaminase (TG) on rennet coagulation properties, and the impact on the properties of processed cheese. The TG was added before, at the same time as, and after the addition of rennet. The parameters moisture, total solids and proteins and physical parameters (syneresis index, wheying‐off and rheological behaviour) were measured. The manufacturing of the milk gels treated with transglutaminase after rennet addition was shown to be an effective means of improving the physical properties (reduced synereseis index and increased consistency index) of processed cheese, possible by the occurrence of enzymatic cross‐linking.     

The effect of supplementing goats milk with whey protein concentrate on textural properties of set-type yoghurt

Yoghurt was manufactured from goat's milk and supplemented with 30 g L^?1 of whey protein concentrate (WPC). The textural properties of the yoghurt were evaluated during the shelf‐life of the product and the textural characteristics of yoghurt made from cow's milk were used as a reference. The instrumental analyses used were the puncture test, stress relaxation test and texture profile analysis. The addition of WPC to goat's milk enhanced the textural characteristics of yoghurt. These advantageous attributes included increased firmness, hardness and adhesiveness. These attributes were quantitatively similar (P > 0.05) to those obtained from yoghurt made from cow's milk. In addition, the textural properties were maintained constant throughout the shelf‐life of the product.