氰戊菊酯残留胶体金免疫层析试纸条研制

应用胶体金免疫层析技术研制出一种准确、快速、简便检测氰戊菊酯农药残留的试纸条.采用柠檬酸三钠还原法制备胶体金,标记氰戊菊酯单克隆抗体,以硝酸纤维素膜为载体,包被氰戊菊酯抗原作为检测线(T线),包被兔抗鼠IgG抗体作为质控线(C线),经过竞争反应后目测结果.实验结果表明,该快速检测试纸条100%抑制浓度为800 ng/m...     

布病胶体金免疫层析检测方法的建立

目的建立诊断布病的胶体金免疫层析方法。方法以B.abortus 544A全菌免疫BALB/c小鼠,以杂交瘤细胞技术制备单抗,纯化后以胶体金标记,选择最佳反应模式组装试纸条,并对其进行鉴定。结果已筛选4B8、1G9、1C9和1E24株稳定分泌单抗的细胞株,以其单抗互相配对组合的试纸条均能检出B.abortus 544A菌。其中以1G9为包被抗体、4B8为金标抗体的试纸检测效果最佳,与以B.abortus 544A菌多抗为包被抗体、4B8单抗为金标抗体的试纸对比,检测结果一致,且不与其他菌发生交叉反应。对PCR检测为阳性的70份牛血清样品,两种试纸条检测结果的符合率均为100%。结论所建立的诊断布病的胶体金免疫层析法快速、简便、准确,有望用于布病的诊断。     

胶体金层析免疫法对盐酸克伦特罗的检测试验

盐酸克伦特罗俗称"瘦肉精",若大量添加到猪饲料中,将在猪肉及内脏中残留,给食品安全带来了隐患。传统的检测技术繁琐,成本高,不利于推广普及。本实验利用胶体金层析法检测盐酸克伦特罗,结果表明:最低检测限度为5 ppb,具有简便、便捷、快速、灵敏度强的特点。     

胶体金免疫层析试纸条在食品安全检测中的研究进展

为了提高免疫检测的速度,简化操作步骤,建立了胶体金免疫层析技术.它是利用纤维膜的毛细作用,使待测物和金标物(抗原/抗体)沿膜表面向前运动,与检测区的捕获物特异结合而聚集显色.它既有免疫学方法的特异性,又有简便快速,无需仪器的优点.本文主要介绍了胶体金免疫层析技术的在食品安全检测中的应用,并对胶体金免疫层析试纸条技术的发...     

胶体金免疫层析法检测小分子物质

在过去的6年里,胶体金及免疫层析试剂越来越多的被用于快速检测上。这一简单免疫层析试剂给很多不同分析物提供了简便而又快速的方法,它的出现给很多工业带来了冲击。最近,胶体金免疫层析法检测小分子物质的报道日益增多,特别是对兽药和农药的检测。文章主要讲述了用于检测小分子物质的胶体金检测试纸的制备原理、检测原理、应用和进展。     

HIV化学发光免疫诊断试剂盒的研制

目的研制敏感、特异的人类免疫缺陷病毒Ⅰ+Ⅱ型(HIVⅠ+Ⅱ)化学发光免疫试剂盒。方法采用碱性磷酶标记重组gp120、gp41和合成肽gp36抗原,金刚烷胺增敏化学发光体系作为酶底物,采用ELISA双抗原夹心法检测。结果敏感度为0.1 ncu;检测范围为0.1~100 ncu;HIV浓度为0.5、1.2、5.1、19.8 ncu时,批内变异系数3%~8%(n=20),批间变异系数5%~9%(n=20);与anti-HAV、anti-HBVa、nti-HCV、anti-HEV无交叉反应;具有良好的稳定性,在1年的效期内(4℃保存)其整体变化幅度<10%。结论该试剂盒灵敏度高,特异性好,稳定性强,检测范围宽,有良好的准确性和重复性。     

胶体金免疫层析法快速检测水果中三氯杀螨醇残留

[目的]建立了快速检测水果中三氯杀螨醇残留的胶体金免疫层析方法.[方法]在对固相载体进行优化的基础上,基于竞争抑制模式建立胶体金免疫层析方法.[结果]该方法对水果中三氯杀螨醇的检出限为1.0 mg/kg,满足国家标准对水果中三氯杀螨醇最大残留限量的要求,且特异性良好.[结论]该方法具有快速、简便、经济实用等特点,适用于...     

D-二聚体胶体金免疫层析定量检测方法中包被浓度和标记浓度对其线性的影响

目的探索不同包被浓度和不同标记浓度对D-二聚体胶体金免疫层析定量检测方法线性的影响。方法采用尿激酶裂解纤维蛋白的方法制备D-二聚体标准品,用所制备D-二聚体试纸条对其在金标定量仪上进行检测以建立标准曲线;用此标准曲线对已用罗氏公司的D-二聚体检测试剂盒(免疫比浊法)定值过的血清进行检测,并对这两种结果进行相关性比较。结果①成功建立了D-二聚体胶体金免疫层析定量检测方法的标准曲线,线性范围为0.15625～10 ug/mL,R~2为0.98297;与罗氏公司免疫比浊法检测方法的相关性良好,相关系数R~2为0.98486;②确定出D-二聚体胶体金免疫层析定量检测方法的最佳标记浓度和最佳包被浓度分别为1 mg/mL和1.5 mg/mL。结论本研究成功建立了D-二聚体胶体金免疫层析定量检测方法的标准曲线,且确定出D-二聚体胶体金免疫层析定量检测方法的最佳的包被浓度和最佳的标记浓度。     

人β-hCG化学发光免疫分析方法的建立

目的建立一种检测人血清hCG浓度的双抗体夹心化学发光免疫分析方法。方法采用2种高特异性的抗β-hCG单克隆抗体,以一种包被微孔板制成固相抗体,另一种标记碱性磷酸酶,以金刚烷衍生物为底物,采用双抗体夹心法检测人血清hCG浓度,并与进口试剂进行比较。结果以5μg/ml单克隆抗体包被微孔板,酶结合物以1∶10000稀释,在室温条件下检测,可测范围为5~540mIU/ml,灵敏度为0.143mIU/ml,批内、批间平均变异系数分别为5.28%和7.05%,平均回收率为98.77%,范围为92.3%~108.6%,与TSH、FSH和LH的交叉反应率均小于0.01%。与国外同类试剂有良好的相关性。结论该方法有较高的灵敏度、精密性和准确性。     

戊型肝炎病毒IgM抗体检测试剂盒制备及功能验证

目的：建立一种快速、简单实用的检测戊型肝炎病毒IgM抗体的胶体金免疫层析法。方法：用柠檬酸三钠还原法制备胶体金颗粒,用鼠源人IgM单克隆抗体进行胶体金标记,采用胶体金免疫层析技术及间接法原理检测戊型肝炎病毒IgM抗体,优化抗体最适宜浓度、抗体稀释度及包被抗原浓度,利用市售ELISA免疫吸附试剂对100例临床血清标本进行比较实验。结果：鼠抗人-IgM抗体标记最适合蛋白用量为10 μg/mL、金标鼠抗人-IgM抗体最适合的稀释度为8%、最佳抗原包被浓度为1.0 mg/mL;与酶联分析法对比,阳性符合率98.1%,阴性符合率100%。结论：HEV-IgM胶体金免疫层析试剂,方法简便、快速,适应戊型肝炎早期感染的快速检测及科研工作需要。     

Preparation of gold catalysts for glucose oxidation

The investigations focused on the influence of doping an alumina support with different base metal oxides on the catalytic performance of gold catalysts to oxidize glucose to gluconic acid. Sodium oxide and calcium oxide strongly enhanced the reaction rate for catalysts prepared by both the deposition–precipitation and incipient wetness method. Urea was used as the precipitation agent in the former. The total selectivity of the catalysts was not influenced by the dopants. TEM analysis revealed very small gold particles of less than 2 nm for sodium doped catalysts prepared by the two methods.     

用于新型冠状病毒快速诊断的荧光免疫层析试纸条的制备及其细胞成像

合成了双光子荧光标记探针LP并对其结构进行了表征,通过LP标记SARS-CoV-2的N-蛋白抗原(Ag)获得LP-Ag,并将LP-Ag制成免疫层析试纸条(TPICS)/试剂盒(TPICK).临床应用研究表明,与核酸法(PCR)/胶体金法比较,TPICS的阳性符合率、阴性符合率和总符合率分别为98.99％/98.10％、...     

Color change of white spot lesions after resin infiltration

This study evaluates color change of the enamel lesion before and after resin infiltration and after thermocycling (TC) in order to assess clinical usefulness of resin infiltrant in restoring esthetics of incipient enamel caries lesions. Twenty‐five healthy premolars were prepared and crown portions were vertically cut. Specimens were emerged in acryl blocks with relatively flat proximal surface facing outside. Specimens were put in decalcification solution (50 mM acetic acid solution, 3 mM CaCl₂·H₂O, 3 mM KH₂PO₄, 6 µM methylhydroxydiphosphonate, pH 4.95, 37°C), and were kept for 160 h, in order to make white spot lesions on the exposed enamel. Degree of decalcification was observed with microscope and nine specimens were excluded because of inadequate decalcification (n = 41). L^*, a^*, and b^* were measured with colorimeter at the baseline (T₁), after production of white spot lesions (T₂), after infiltration of resin infiltrants (ICON^®, DMG, Hamburg, Germany) (T₃), after 3000 (T₄), 6000 (T₅), and 10,000 cycles (T₆) of TC. L^* was highest at T₂, but significantly decreased until T₆ which was the closest value to T₁ (P < 0.05). a^* was highest at T₂ and decreased gradually until T₆, whereas b^* was lowest at T₂ and was highest at T₃ and decreased gradually until T₆. Δ less than 3.7 showed between T₁ and T₄, between T₁ and T₅, and between T₁ and T₆, which suggested that it was hard to differentiate one from the other clinically. Resin infiltrated lesions showed recovery of CIE value close to that of healthy enamel over time. © 2013 Wiley Periodicals, Inc. Col Res Appl, 39, 506–510, 2014     

呋喃唑酮代谢物单克隆抗体及其新型免疫层析检测试纸条的制备

目的制备呋喃唑酮代谢物AOZ单克隆抗体及其胶体金与荧光淬灭免疫层析试纸条。方法将AOZ衍生成CPAOZ后,与载体蛋白BSA偶联,制备完全抗原CPAOZ-BSA,经皮下多点注射免疫BALB/c小鼠,共5次,取小鼠脾细胞与SP2/0细胞融合,获得稳定分泌特异性抗体的杂交瘤细胞株。利用获得的抗CPAOZ单克隆抗体制备胶体金及荧光淬灭免疫层析试纸条,并对试纸条的灵敏度及特异性进行验证。结果制备的完全抗原经紫外扫描鉴定偶联成功。共获得3株能稳定分泌抗CPAOZ单克隆抗体的杂交瘤细胞株,其中效价和特异性最好的1株2H11针对CPAOZ的50%抑制质量浓度(IC50)为0.88 ppb。以该抗体制备的胶体金及荧光淬灭免疫层析试纸条的最低检测限分别为7.5和0.062 5 ppb;两种试纸条与其他3种硝基呋喃类代谢物CPAMOZ、CPAHD、CPSEM均不存在交叉反应。结论成功制备了抗CPAOZ单抗及其胶体金免疫层析和荧光淬灭免疫层析试纸条,两种试纸条均具有较高的灵敏度和较强的特异性,其中荧光淬灭免疫层析试纸条的灵敏度较胶体金免疫层析试纸条高约100倍,具有良好的应用前景。     

Preparation of graphite composite bipolar plate for PEMFC

This research studied the preparation of graphite composite using liquid thermosetting plastic such as polyester resin (POE), phenolic modified alkyd resin (PhA) and mixed resin (POE with 10% PhA) as a binder. The morphology, physical, electrical and mechanical properties of the graphite composites were analyzed. The results showed that POE could combine with graphite powder (the 66% wt. saturated of graphite powder) better than PhA and mixed resin and gave higher electrical conductivity (4.52 S/cm). It was also found that epoxy resin could improve the mechanical property of composite plate. The addition of TiO₂ and ZnSt slightly decreased the electrical conductivity and the water absorption. Moreover, it was proposed that TiO₂ could improve the mechanical property. Carbon fiber can increase electrical and mechanical properties and water absorption of the composite with POE as a binder. The mixing of wet-lay mixture with graphite, carbon fiber and POE composite improved the mechanical property and decreased the water absorption.     

Up-conversion phosphor plate for white lighting device using NIR excitation source

Upconversion phosphors have numerous advantages compared to down conversion phosphor materials, such as a weak background interface, low excitation energy, sharp emission lines, and long lifetimes. Conventionally, blue laser diodes are used to obtain strong white light by combining green- and red-emitting phosphors. The blue-excited white-light-emitting devices can be harmful when the blue light penetrates directly into the human body. However, lower energy excited lighting devices does not harm the human body because of their low-energy photons are compatible with the biologically safe window. Hence, we present a prototype device that converts invisible near-infrared light into visible white light. For this, we synthesized orange-emitting Y₂O₃:Er³⁺, Yb³⁺ and blue-emitting Y₂O₃:Tm³⁺, Yb³⁺ upconversion phosphors using a solid-state reaction. Using these, phosphor-in-glass (PiG) samples with sintered glass frit were prepared to investigate their optical behavior under low-excitation energy. The emission color from the stacked PiGs depends on the color balance between the activator ions of Er³⁺, Tm³⁺, and Yb³⁺, and their balance is optimized to obtain a white light. The results might pave the way for designing safe white-light-emitting devices using a low-energy excitation source.     

加氢法生产白色环烷基橡胶油的研究

研究加氢法生产白色环烷基橡胶油的工艺条件及产品性能。加氢工艺路线为原料油→高压加氢处理→临氢降凝→切割→高压加氢脱芳烃→产品。以稠油减二线馏分油生产的橡胶油KFW4006的日光稳定性和紫外光稳定性均优于采用传统磺化工艺生产的食品级商业石蜡基和环烷基参比油；以稠油减三线馏分油生产的橡胶油KFW4010的日光稳定性和紫外光稳定性均与参比油相当；两种橡胶油的热稳定性均优于参比油。填充橡胶油KFW4006和KFW4010的苯乙烯-丁二烯-苯乙烯嵌段共聚物（SBS）充油胶的邵尔A型硬度、300％定伸应力、拉伸强度、拉断永久变形和熔融指数均比填充石蜡基参比油的SBS充油胶有所增大，且各项性能均达到国家标准要求。     

木器用PU哑光白面漆的研制

针对木器用PU哑光白面漆脆裂和黄变的突出问题,分析了其造成的原因,并通过对树脂和固化剂的选择,以及颜填料、光稳定剂的搭配制得了具有较高性价比的产品,并经过了市场广泛的认可。     

隔膜滤板膜片用改性聚丙烯的制备

研究了聚丙烯品种、弹性体和生产工艺对改性聚丙烯微观结构、力学性能的影响,开发了3种具有不同性能特点的隔膜滤板膜片用改性聚丙烯。结果表明:产品具有优异的拉伸强度、断裂拉伸应变和耐温性能;成型的膜片经过高压充水多次开合不发生破裂,满足隔膜式压滤机的使用要求。