Preparation and characterization of acrylic acid/itaconic acid hydrogel coatings containing silver nanoparticles

Hydrogel silver nanocomposites have been used in applications with excellent antibacterial performance. Acrylic acid (AA)/itaconic acid (IA) hydrogels silver nanocomposites were prepared and applied as a coating on a textile substrate. Hydrogel matrices were synthesized first by the polymerization of an AA/IA aqueous (80/20 v/v) solution and mixed with 2‐2‐azobis(2‐methylpropionamide) diclorohydrate and N,N′‐methylene bisacrylamide until the hydrogel was formed. Silver nanoparticles were generated throughout the hydrogel networks with an in situ method via the incorporation of the silver ions and subsequent reduction with sodium borohydride. Cotton (C) and cotton/polyester (CP) textile fibers were then coated with these hydrogel silver nanocomposites. The influence of these nanocomposite hydrogels on the properties of the textile fiber were investigated by infrared spectroscopy (attenuated total reflectance), scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, and antibacterial tests against Pseudomona aeruginosa and Staphylococcus aureus. The better conditions, in which no serious aggregation of the silver nanoparticles occurred, were determined. It was proven that the textiles coated with hydrogels containing nanosilver had an excellent antibacterial abilities. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 2713–2721, 2013     

Preparation, characterization, and antibacterial properties of pH-responsive P(MMA-co-MAA)/silver nanocomposite hydrogels

A pH-responsive copolymer hydrogel was synthesized based on methyl methacrylate (MMA) and methacrylic acid (MAA) as monomers, and was adopted as a nanoreactor for assembling Ag nanoparticles. Fourier transform infrared spectroscope (FTIR), scanning electron microscopy (SEM), transmission electron microscope (TEM), UV-visible spectroscopy (UV-Vis) and thermogravimetric analysis (TGA) were used to fully characterize the formation of silver nanoparticles in P(MMA-co-MAA) hydrogels. The experimental results showed that the P(MMA-co-MAA) hydrogels assume a three-networks architecture in morphologies, and that nearly spherical Ag nanoparticles are formed in these hydrogel networks; the size of these Ag nanoparticles varies with the system composition. The swelling kinetics investigations demonstrated that the equilibrium swelling ratio (ESR) of the P(MMA-co-MAA)/Ag hydrogels depended on the content of the MAA and pH of the buffer solutions, and the ESR values were reduced with increasing MAA contents. The antibacterial properties against both S. aureus and B. subtilis bacteria demonstrated that the P(MMA-co-MAA)/silver nanocomposite hydrogels had higher antimicrobial efficacy than the pure P(MMA-co-MAA) counterparts. Therefore, the nanocomposite hydrogels turned out to be a potentially smart material in the range of applications of antibacterial activity.     

Silver/poly(N‐vinyl‐2‐pyrrolidone) hydrogel nanocomposites obtained by electrochemical synthesis of silver nanoparticles inside the polymer hydrogel aimed for biomedical applications

Silver nanoparticles were produced inside a poly(N‐vinyl‐2‐pyrrolidone) hydrogel (PVP) by an innovative method based on the electrochemical reduction of Ag⁺ ions within the swollen PVP hydrogel. UV‐visible spectroscopy showed the highest value of the absorbance intensity and the lowest values of the wavelength of the absorbance maximum and the full width at the half‐maximum absorbance for the Ag/PVP nanocomposite obtained at 200 V during 4 min. Cyclic voltammetry results suggested an adequate entrapment of the silver nanoparticles. The mechanical properties under bioreactor conditions of the Ag/PVP nanocomposite suggested the possibility of wound dressing application. Silver release from Ag/PVP nanocomposites was confirmed under static conditions as well as by their antimicrobial activity against Staphylococcus aureus. POLYM. COMPOS., 35:217–226, 2014. © 2013 Society of Plastics Engineers     

Radiation synthesis and characterization of poly(vinyl alcohol)/poly(N-vinyl-2-pyrrolidone) based hydrogels containing silver nanoparticles

A series of PVA/PVP based hydrogels at different compositions were prepared by gamma irradiation. The gel fraction degree of swelling were investigated. Highly stable and uniformly distributed silver nanoparticles have been obtained onto hydrogel networks. The morphology and structure of (PVA/PVP) hydrogel and dispersion of the silver nanoparticles in the polymeric matrix were examined by scanning electron microscopy (SEM) and infrared spectroscopy (FT-IR), respectively. The formation of silver nanoparticles has been confirmed by ultraviolet visible (UV–vis) spectroscopy. A strong characteristic absorption peak was found to be around 420 nm for the silver nanoparticles in the hydrogel nanocomposite. The X-ray diffraction pattern confirmed the formation of silver nanoparticles with average particle size of 12 nm. The diameter distribution of silver nanoparticles was determined by dynamic light scattering DLS. Transmission electron microscope (TEM) showed almost spherical and uniform distribution of silver nanoparticles through the hydrogel network and the mean size of silver nanoparticles ranging is 23 nm. The good swelling properties and antibacterial of PVA/PVP-Ag hydrogel suggest that it can be a good candidate as wound dressing.     

Preparation and characterization of cotton fibers coated with AA-IA hydrogel containing silver/graphene or graphene oxide nanoparticles

This study presents the fabrication and characterization of cotton textile fibers coated with hydrogels containing silver and Graphene or Graphene Oxide nanoparticles using 1-hexyl-3-methyl-imidazolium (HMIMPF6) ionic liquid (IL) as carbon filler dispersant. Acrylic acid/Itaconic acid (AA-IA) hydrogels are synthesized by polymerizing an acrylic acid-itaconic acid aqueous (80/20 v/v) solution and mixed with 2-2-Azobis (2-methylpropionamide) diclorohydrate, and N,N´-methylenbis (acrylamide). Then silver nanoparticles are generated throughout the hydrogel networks using in situ method by incorporating the silver ions and subsequent reduction with sodium borohydride. Then a cotton textile fiber substrate was coated with this hydrogel. Finally, graphene or graphene oxide was added to the textile substrate already impregnated with hydrogel and silver nanoparticles. In order to favor the dispersion of the carbon nano-structures in the system, an IL was used. The influence of these nanocomposite hydrogels on the properties of textile fiber were investigated by infrared spectroscopy (ATR), scanning electron microscopy (SEM), inductively coupled plasma mass spectroscopy (ICP) and antibacterial tests against Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative). The effect of each and combined fillers dispersion on antimicrobial properties were determined. Cotton fibers coated with hydrogel containing silver nanoparticles and graphene showed better results when the ionic liquid was used. Graphene showed greater antimicrobial efficiency than graphene oxide. It was proved that the textiles coated with hydrogels containing these fillers had an excellent antibacterial ability and are a good option to be used for medical applications such as wounds and burns dressing.     

In vitro and in vivo investigational studies of a nanocomposite‐hydrogel‐based dressing with a silver‐coated chitosan wafer for full‐thickness skin wounds

A nanocomposite reservoir‐type hydrogel dressing of poly vinyl alcohol (PVA) was fabricated by a freeze–thaw method and loaded with silver‐nanoparticle‐coated chitosan wafers (Ag–CHWs). The Ag–CHWs were synthesized by a sonication technique with silver nitrate (AgNO₃) and chitosan powder. Scanning electron microscopy images showed silver nanoparticles (AgNPs) with a size range of 10 ± 4 nm on the surface of the chitosan wafers, and the antibacterial efficacy (minimum inhibitory concentration) of the Ag–CHWs was measured against Pseudomonas aeruginosa (32 µg/mL), Staphylococcus aureus, (30 µg/mL) and Escherichia coli (32 µg/mL). The antimicrobial PVA hydrogel showed an improved tensile strength (～0.28 MPa) and gel content (～92%) in comparison with the blank hydrogels. Full‐thickness‐excision wound studies of the nanocomposite dressing on Wistar rats revealed enhanced wound contraction, improved inflammation response, re‐epithelization rate, neoangiogenesis, and granulation tissue formation in comparison to the control group. A flexible, biocompatible, nanocomposite reservoir dressing not only established the chitosan as a stabilizer but also proved the efficacious and safe utility of AgNPs toward chronic wound management. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133, 43472.     

Gamma irradiation synthesis and characterization of AgNP/gelatin/PVA hydrogels for antibacterial wound dressings

An antibacterial hydrogel wound dressing was successfully synthesized by the gamma irradiation method. A gelatin solution was mixed with a poly(vinyl alcohol) (PVA) solution of similar concentrations at different weight ratios of 100 : 0, 80 : 20, and 60 : 40 w/w, and irradiated at 30, 40, or 50 kGy. The testing of physical properties showed that the addition of PVA could improve both durability and mechanical integrity. The 60 : 40 hydrogels irradiated at 30 kGy were optimal, and chosen to add silver nitrate at 0.25, 0.50, 0.75, or 1.00 wt % (based on the solid content) to improve the antibacterial properties. After gamma irradiation, silver nanoparticles (AgNPs) were formed. The AgNP/gelatin/PVA hydrogels were characterized for physical properties, cytotoxicity, and antibacterial activity. The AgNP/gelatin/PVA hydrogels could be used as antibacterial wound dressings because they exhibited appropriate physical properties, noncytotoxicity, and could inhibit the growth of tested bacteria. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 41138.     

Fabrication of Au and Ag Bi-metallic nanocomposite for antimicrobial applications

Hydrogel silver nanocomposites have shown immense potential in many biomedical applications, specifically wound healing. The combination of bi-metallic (Ag, Au) hydrogel nanocomposites are developed to enhance their antimicrobial activity. This paper presents the fabrication of bi-metallic nanocomposites obtained from the synthesis of acrylamide (AM) and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) based hydrogels for antimicrobial applications. The nanocomposite formation was confirmed by scanning electron microscopy (SEM), thermal analysis (TGA/DSC), as well as X-ray diffraction (XRD) methods. The bi-metallic nanocomposite hydrogel has shown significant antibacterial activity on bacillus. Therefore, these bi-metallic antibacterial materials are promising candidates for a wide range of biomedical applications. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012     

Temperature/pH dual responsive OPGMA based copolymeric hydrogels prepared by gamma radiation: an optimisation study

The purpose of this study was to attain oligo(propylene glycol) methacrylate (OPGMA) based hydrogels for different biomedical applications. Since the volume phase transition temperature (VPTT) of poly(oligo(propylene glycol) methacrylate) (POPGMA) homopolymer was below room temperature, it was necessary to tune the thermoresponsiveness and the VPTT to higher temperatures; radical copolymerisation of OPGMA with more hydrophilic and/or pH responsive monomers was used to adjust these values. A series of copolymeric hydrogels with different ratios of OPGMA, 2-hydroxyethyl methacrylate (HEMA) and itaconic acid (IA) was synthesised by gamma radiation. The swelling properties were preliminarily investigated over a wide pH (2.2–9.0) and temperature (4–80 °C) ranges. Additional characterization of their structure and properties was conducted by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). The presented results revealed temperature/pH dual responsive P(OPGMA/IA) and P(OPGMA/HEMA/IA) copolymers with large diversity in swelling properties, as a result of the addition of OPGMA thermoresponsive units with a lower critical solution temperature (LCST) and IA pH responsive units. It was demonstrated that VPTT can be significantly shifted to higher (e.g. body) temperatures at higher pH values (pH ≥ 4) by increasing the IA content in the hydrogel composition. Due to the possibility of making dual responsive copolymers in the manner explained in this paper, OPGMA based hydrogels show potential for different biomedical applications.     

Collagen-based hydrogel films as drug-delivery devices with antimicrobial properties

Collagen (coll)-containing hydrogel films were prepared by mixing degraded collagen with monomers such as acrylamide (AAm), and 2-hydroxy ethylmethacrylate (HEMA) before the polymerization/cross-linking of composites as p(coll-co-AAm), and p(coll-co-HEMA), respectively. These materials were used as drug-delivery devices for potential wound dressing materials by loading and releasing of model drugs such as gallic acid (GA) and naproxen (NP). A linear release profile was obtained up to 32-h release from GA-loaded p(coll-co-AAm) interpenetrating polymeric networks films, and 36-h linear release profile of NP for p(coll-co-HEMA). Furthermore, metal nanoparticles such as Ag and Cu prepared within these hydrogel films offered antimicrobial characteristic against known common bacteria such as Escherichia coli, Bacillus subtilis, and Staphylococcus aureus.     

类氨基酸基载药水凝胶敷料的制备与性能

为制备一种具有良好生物相容性、可控缓释的物理交联的水凝胶敷料,选用类氨基酸单体N-丙烯酰基甘氨酰胺（NAGA）与生物发酵产物衣康酸（IA）为单体,在紫外光条件下,通过自由基聚合,在不需要外加任何交联剂条件下即可形成水凝胶聚（N-丙烯酰基甘氨酰胺-衣康酸）（P(NAGA-IA)）。所得水凝胶具有溶胶-凝胶转变温度（UCST）、较高的水溶胀率（40倍）及力学性能（压缩模量最高540 kPa）、较优的药物负载性和缓释性,这是因为NAGA单元提供分子间多重氢键作用,进而赋予了水凝胶较优的综合性能;而IA单元赋予了聚合物的pH刺激响应性,从而可诱导药物的释放。因此,所得P (NAGA-IA)水凝胶可作敷料用于创伤治疗。     

Synthesis and characterization of silver nanoparticle incorporated gelatin‐hydroxypropyl methacrylate hydrogels for wound dressing applications

A replaceable wound cover which absorbs moisture and resist infection can be used to prevent development of chronic wounds. A major criterion for a replaceable wound dressing is nonadherence to cells to prevent pain upon removal. A major limitation of water absorbing hydrogels used in wound dressing applications is their poor mechanical strength. In this study, gelatin methacrylate (GelMA) was synthesized by reacting Type A porcine skin gelatin with methacrylic anhydride at 50 °C. Resultant GelMA monomer containing polyethylene glycol (PEG) protected silver nanoparticles were subsequently copolymerized with 2‐hydroxypropyl methacrylate (HPMA) at room temperature by redox mechanism. This resulted in a hydrogel copolymer with optimum mechanical stability and moisture retention while inhibiting microbial contamination and FT‐IR spectroscopy was used to confirm copolymer formation. Antimicrobial properties of the hydrogel using agar diffusion showed zone of inhibition against Staphylococcus aureus. Surface morphology was observed using scanning electron microscopy (SEM) and elemental analysis was carried out using energy‐dispersive spectroscopy (EDS). Micro‐computed tomography (micro‐CT) analysis of the hydrogel showed enhancement in the pore size from around 32 µ to 48–64 µ after incorporation of silver nanoparticles. Degradation of the hydrogel was observed after 48 h when stored in PBS containing collagenase enzyme. In vitro cell culture experiments established absence of cytotoxicity in the hydrogel and nonadherence character to dermal fibroblasts. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134, 44529.     

Development and Characterization of Highly Stable Silver NanoParticles as Novel Potential Antimicrobial Agents for Wound Healing Hydrogels

Recurrent microbial infections are a major cause of surgical failure and morbidity. Wound healing strategies based on hydrogels have been proposed to provide at once a barrier against pathogen microbial colonization, as well as a favorable environment for tissue repair. Nevertheless, most biocompatible hydrogel materials are more bacteriostatic than antimicrobial materials, and lack specific action against pathogens. Silver-loaded polymeric nanocomposites have efficient and selective activity against pathogenic organisms exploitable for wound healing. However, the loading of metallic nanostructures into hydrogels represents a major challenge due to the low stability of metal colloids in aqueous environments. In this context, the aim of the present study was the development of highly stable silver nanoparticles (AgNPs) as novel potential antimicrobial agents for hyaluronic acids hydrogels. Two candidate stabilizing agents obtained from natural and renewable sources, namely cellulose nanocrystals and ulvan polysaccharide, were exploited to ensure high stability of the silver colloid. Both stabilizing agents possess inherent bioactivity and biocompatibility, as well as the ability to stabilize metal nanostructures thanks to their supramolecular structures. Silver nitrate reduction through sodium borohydride in presence of the selected stabilizing agents was adopted as a model strategy to achieve AgNPs with narrow size distribution. Optimized AgNPs stabilized with the two investigated polysaccharides demonstrated high stability in phosphate buffer saline solution and strong antimicrobial activity. Loading of the developed AgNPs into photocrosslinked methacrylated hyaluronic acid hydrogels was also investigated for the first time as an effective strategy to develop novel antimicrobial wound dressing materials.     

Hydrogels containing silver nanoparticles for burn wounds show antimicrobial activity without cytotoxicity

This research introduces a novel dressing for burn wounds, containing silver nanoparticles in hydrogels for infected burn care. The 2‐acrylamido‐2‐methylpropane sulfonic acid sodium salt hydrogels containing silver nanoparticles have been prepared via ultraviolet radiation. The formation of silver nanoparticles was monitored by surface plasmon bands and transmission electron microscopy. The concentration of silver nitrate loaded in the solutions slightly affected the physical properties and mechanical properties of the neat hydrogel. An indirect cytotoxicity study found that none of the hydrogels were toxic to tested cell lines. The measurement of cumulative release of silver indicated that 70%–82% of silver was released within 72 hr. The antibacterial activities of the hydrogels against common burn pathogens were studied and the results showed that 5 mM silver hydrogel had the greatest inhibitory activity. The results support its use as a potential burn wound dressing. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40215.     

Preparation and drug‐release behavior of minocycline‐loaded poly[hydroxyethyl methacrylate‐co‐poly(ethylene glycol)–methacrylate] films

In this work, biocompatible hydrogel matrices for wound‐dressing materials and controlled drug‐release systems were prepared from poly[hydroxyethyl methacrylate‐co‐poly(ethylene glycol)–methacrylate] [p(HEMA‐co‐PEG–MA] films via UV‐initiated photopolymerization. The characterization of the hydrogels was conducted with swelling experiments, Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis (differential scanning calorimetry), and contact‐angle studies. The water absorbency of the hydrogel films significantly changed with the change of the medium pH from 4.0 to 7.4. The thermal stability of the copolymer was lowered by an increase in the ratio of poly(ethylene glycol) (PEG) to methacrylate (MA) in the film structure. Contact‐angle measurements on the surface of the p(HEMA‐co‐PEG–MA) films demonstrated that the copolymer gave rise to a significant hydrophilic surface in comparison with the homopolymer of 2‐hydroxyethyl methacrylate (HEMA). The blood protein adsorption was significantly reduced on the surface of the copolymer hydrogels in comparison with the control homopolymer of HEMA. Model antibiotic (i.e., minocycline) release experiments were performed in physiological buffer saline solutions with a continuous flow release system. The amount of minocycline release was shown to be dependent on the HEMA/PEG–MA ratio. The hydrogels have good antifouling properties and therefore are suitable candidates for wound dressing and other tissue engineering applications. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009     

Characterization and In Vitro Drug Release Behavior of (2-hydroxyethyl methacrylate)–co-(2-acrylamido-2-methyl-1-propanesulfonic acid) Crosslinked Hydrogels Prepared by Ionizing Radiation

A series of copolymer hydrogels of 2-hydroxyethyl methacrylate (HEMA)/2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) were prepared by solution free radical polymerization using gamma irradiation. Fourier transform infrared spectroscopy, thermogravimetric analysis, and scanning electron microscopy techniques were used to characterize the HEMA/AMPS hydrogels. The swelling behavior of the prepared hydrogels was determined by investigating the time and pH-dependent swelling of the HEMA/AMPS hydrogels of different AMPS content. The drug loading and controlled release behaviors of the hydrogel were evaluated using hydrocortisone acetate as drug model. The results indicated that the release rate of the drug from the hydrogel showed good pH-response.     

Development of microbial protective Kolliphor‐based nanocomposite hydrogels

In this scientific work, a novel class of antimicrobial nanocomposite hydrogels were designed and synthesized by chemical and environmentally bioprocess using Kolliphor, acrylamide, and mint leafs in order to achieve antiseptic property for wound applications. In the bioprocess approach, silver nitrate and gold chloride were nucleated with mint leafs in order to obtain effective free individual nano‐inorganic compounds to provide superior antibacterial assets. The formations of dual inorganic nanoparticles were confirmed by transmission electron microscopy, which indicated the size of nanoparticles in the range of approximately 3 ± 2 nm and without agglomeration. The formations of biomaterials were characterized using Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopic–energy dispersive spectrometric studies and their swelling properties were determined. Furthermore, the pure hydrogel and the dual inorganic nanocomposite hydrogels developed were tested for antibacterial activities. When compared with the neat hydrogel, the nanocomposite hydrogels significantly improved their anti‐bacterial activities on Bacillus bacterium. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 42781.     

Ultrasmall silver nanoparticles loaded in alginate–hyaluronic acid hybrid hydrogels for treating infected wounds

Nowadays, silver nanoparticles are in the limelight to control infection during wound healing process, and a vast variety of antimicrobial dressings based on colloidal silver have been marketed to fight wound invasion of pathogen bacteria, which represents one of the main adverse effects limiting the repair process. Here we propose a biofunctional hydrogel based on alginate (ALG) and hyaluronic acid (HA) embedding ultrasmall silver nanoparticles (usSN, <1?nm) as antimicrobial component. The hydrogels were fabricated in different size by a straightforward internal gelation method using CaCO₃ and glucono-δ-lactone. To follow usSN release from the hydrogels in aqueous media, catalytic activity of usSN-loaded hydrogels was evaluated. Results suggested that catalytic activity was low in intact hydrogels and high when hydrogels dissolved, which suggests that usSN firmly interact with polymer chains and are available in the medium depending on the extent of hydrogel degradation. HA-containing hydrogels showed faster dissolution in simulated physiological conditions and higher antibiofouling properties as compared to hydrogels made only of ALG. Free usSN were not toxic toward human mesenchymal stem cells (Ad-MSCs), previously isolated from subcutaneous adipose tissue biopsies, up to 50?µg/mL. At this concentration, viability of Ad-MSCs was unaffected whereas their motility was significantly higher as compared to control (p<0.01) for both free usSN and hydrogel integrating. Antimicrobial activity on clinical isolates of both Gram-positive and Gram-negative bacteria demonstrated that usSN at 50?µg/mL were able to kill all the bacteria tested after 24 and 48?h of contact time. In the case of hydrogels, a matrix effect was found and bacterial killing was significant only at 24?h and dependent on bacterial strain, being Gram-negative bacteria more susceptible. These results clearly indicate that usSN interaction with polymer network and exposure time can strongly affect usSN antimicrobial profile in the hydrogel and, in turn, timing of hydrogel change at injured site in a clinical setting. On the whole, ALG/HA hydrogels integrating usSN can be considered a suitable option to fabricate biofunctional dressings for hospitalized patients and worth of further in vivo investigation.     

Development of Gelatin Based Inorganic Nanocomposite Hydrogels for Inactivation of Bacteria

In this investigation, silver nanocomposite hydrogels were developed by using acrylamide and biodegradable gelatin. Silver nanoparticles were generated throughout the hydrogel networks using in situ method by incorporating Ag⁺ ions and the subsequent treatment with sodium borohydride. The effect of gelatin on the swelling studies was investigated. The hydrogel synthesized silver nanocomposites were characterized by using Fourier transform infrared, UV–Visible spectroscopy, X-ray diffraction, thermogravimetric analysis, scanning electron and transmission electron microscopy techniques. The biodegradable gelatin-based silver nanocomposite hydrogels were tested for antibacterial properties. The results indicate that these biodegradable silver nanocomposite hydrogels can be useful in medical applications, as antibacterial agents.     

Adsorption of Ag (I) Ions from Aqueous Solutions Using Photocured Thiol-Ene Hydrogel

In this present work, we reported the adsorption and recovery studies of a P(Penta3MP4/PEG-DA/HEMA) thiol-ene based hydrogel. Real-time infrared spectroscopy technique was used to identify the photopolymerization kinetics of thiol-ene based formulations. The chemical composition and surface morphology of hydrogels were also characterized. The influence of the adsorption conditions such as pH, hydrogel formulations, contact time, initial metal ion concentration, and foreign metal ions on the metal ion binding capacity of hydrogel, were tested. Both the Langmuir and Freundlich isotherm models were applied to the method. The reusability of the hydrogels and the usability of the hydrogels for preconcentration studies were also investigated. The analytical parameters of the method were calculated and the recovery of silver ions from waste radiographic films was also applied to the hydrogels.