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1.
Malakiba Sen Dipak Kumar Bhattacharyya 《European Journal of Lipid Science and Technology》2000,102(12):727-733
The effect of removing anti‐nutritional factors from n‐hexane‐extracted mustard meal using 80% isopropanol (to reduce thioglucosides, phenolics, etc.) on growth, food efficiency ratio, serum and liver lipid profiles and protein content of young rats was examined. For this n‐hexane‐extracted mustard meal was extracted with 80% isopropanol giving a fraction with 68% protein and low residual thioglucoside (0.5%) as well as phenolic (0.3%) content. This isopropanol‐extracted mustard seed protein fraction reduced the growth of young rats slightly when compared with casein. The food efficiency ratio between rats fed isopropanol‐extracted mustard seed protein or casein did not differ, nor did the protein composition affect serum total cholesterol, triglyceride, HDL‐cholesterol, LDL‐cholesterol, VLDL‐cholesterol and LDL‐C/HDL‐C ratio. However, rats fed isopropanol‐extracted mustard seed protein showed a significantly lower (p<0.05) liver cholesterol concentration than rats fed casein. Liver triglyceride and phospholipid concentrations did not differ between rats fed the two proteins, nor was serum protein affected. This study indicates that extraction of hexane‐extracted mustard meal with 80% isopropanol reduced a number of anti‐nutritional factors like thioglucoside and phenolics. Furthermore the nutritional quality of mustard seed protein fraction is comparable to casein in respect to growth, food efficiency ratio, serum lipid and protein concentrations and organ weights. 相似文献
2.
Rosuvastatin Enhances the Catabolism of LDL apoB‐100 in Subjects with Combined Hyperlipidemia in a Dose Dependent Manner
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Ngoc‐Anh Le Margaret R. Diffenderfer Nuntakorn Thongtang Esther M. M. Ooi P. Hugh R. Barrett Katalin V. Horvath Gregory G. Dolnikowski Bela F. Asztalos Ernst J. Schaefer W. Virgil Brown 《Lipids》2015,50(5):447-458
Dose‐associated effects of rosuvastatin on the metabolism of apolipoprotein (apo) B‐100 in triacylglycerol rich lipoprotein (TRL, d < 1.019 g/ml) and low density lipoprotein (LDL) and of apoA‐I in high density lipoprotein (HDL) were assessed in subjects with combined hyperlipidemia. Our primary hypothesis was that maximal dose rosuvastatin would decrease the apoB‐100 production rate (PR), as well as increase apoB‐100 fractional catabolic rate (FCR). Eight subjects received placebo, rosuvastatin 5 mg/day, and rosuvastatin 40 mg/day for 8 weeks each in sequential order. The kinetics of apoB‐100 in TRL and LDL and apoA‐I in HDL were determined at the end of each phase using stable isotope methodology, gas chromatography‐mass spectrometry, and multicompartmental modeling. Rosuvastatin at 5 and 40 mg/day decreased LDL cholesterol by 44 and 54 % (both P < 0.0001), triacylglycerol by 14 % (ns) and 35 % (P < 0.01), apoB by 30 and 36 % (both P < 0.0001), respectively, and had no significant effects on HDL cholesterol or apoA‐I levels. Significant decreases in plasma markers of cholesterol synthesis and increases in cholesterol absorption markers were observed. Rosuvastatin 5 and 40 mg/day increased TRL apoB‐100 FCR by 36 and 46 % (both ns) and LDL apoB‐100 by 63 and 102 % (both P < 0.05), respectively. HDL apoA‐I PR increased with low dose rosuvastatin (12 %, P < 0.05) but not with maximal dose rosuvastatin. Neither rosuvastatin dose altered apoB‐100 PR or HDL apoA‐I FCR. Our data indicate that maximal dose rosuvastatin treatment in subjects with combined hyperlipidemia resulted in significant increases in the catabolism of LDL apoB‐100, with no significant effects on apoB‐100 production or HDL apoA‐I kinetics. 相似文献
3.
The Mongolian gerbil,Meriones unguiculatus, may be a suitable animal model for the investigation of dietary lipid effects on cholesterol metabolism. The effects of
dietary cholesterol, and its possible interaction with the type of dietary fat, on the lipoprotein status of this animal have
not been examined previously. In the present research, the effects of adding 0.5% cholesterol to diets high in saturated (19.5%
beef tallow: 0.5% safflower oil) or polyunsaturated (20% safflower oil) fats on the lipoprotein status of the gerbil were
determined after 11 and 22 days of feeding. Lipoproteins (VLDL, LDL and HDL) were separated by sequential ultracentrifugation.
Their cholesterol, phospholipid and protein concentrations were determined colorimetrically. In the absence of 0.5% cholesterol,
safflower oil lowered the concentration (mg/100 ml) of cholesterol in each of the VLDL, LDL and HDL relative to beef tallow
(BT) without greatly influencing the cholesterol distribution amongst them. The HDL carried the majority of the serum cholesterol
and the VLDL transported the smallest amount. However, inclusion of 0.5% dietary cholesterol resulted in a redistribution
of cholesterol amongst the lipoproteins so that the VLDL and LDL became the major and the HDL the minor carriers. Dietary
cholesterol also brought about a rise in the VLDL and LDL concentrations (mg/100 ml) of cholesterol, phospholipid and protein
and altered the VLDL and LDL compositions. No such changes were observed in the HDL, indicating that the HDL are relatively
resistant to any of the possible effects of cholesterol feeding measured in this experiment. The specific mechanisms responsible
for the changes observed in the lipoprotein status of the gerbil remain to be elucidated.
Presented in part at the Triennial Joint Meeting of the AIN/ASCN/CSNS, July 1982 相似文献
4.
This experiment was designed to evaluate the effects of dietary red wine phenolic compounds (WP) and cholesterol on lipid
oxidation and transport in rats. For 5 wk, weanling rats were fed polyunsaturated fat diets (n−6/n−3=6.4) supplemented or
not supplemented with either 3 g/kg diet of cholesterol, 5 g/kg diet of WP, or both. The concentrations of triacylglycerols
(TAG, P<0.01) and cholesterol (P<0.0002) were reduced in fasting plasma of rats fed cholesterol despite the cholesterol enrichment of very low density lipoprotein
+ low density lipoprotein (VLDL+LDL). The response was due to the much lower plasma concentration of high density lipoprotein
(HDL) (−35%, P<0.0001). In contrast, TAG and cholesteryl ester (CE) accumulated in liver (+120 and +450%, respectively, P<0.0001). However, the cholesterol content of liver microsomes was not affected. Dietary cholesterol altered the distribution
of fatty acids mainly by reducing the ratio of arachidonic acid to linoleic acid (P<0.0001) in plasma VLDL+LDL (−35%) and HDL (−42%) and in liver TAG (−42%), CE (−78%), and phospholipids (−28%). Dietary WP
had little or no effect on these variables. On the other hand, dietary cholesterol lowered the α-tocopherol concentration
in VLDL+LDL (−40%, P<0.003) and in microsomes (−60%, P<0.0001). In contrast, dietary WP increased the concentration in microsomes (+21%, P<0.0001), but had no effect on the concentration in VLDL+LDL. Cholesterol feeding decreased (P<0.006) whereas WP feeding increased (P<0.0001) the resistance of VLDL+LDL to copper-induced oxidation. The production of conjugated dienes after 25 h of oxidation
ranged between 650 (WP without cholesterol) and 2,560 (cholesterol without WP) μmol/g VLDL+LDL protein. These findings show
that dietary WP were absorbed at sufficient levels to contribute to the protection of polyunsaturated fatty acids in plasma
and membranes. They could also reduce the consumption of α-tocopherol and endogenous antioxidants. The responses suggest that,
in humans, these substances may be beneficial by reducing the deleterious effects of a dietary overload of cholesterol. 相似文献
5.
Postprandial Lipid Responses do not Differ Following Consumption of Butter or Vegetable Oil when Consumed with Omega-3 Polyunsaturated Fatty Acids
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Dietary saturated fat (SFA) intake has been associated with elevated blood lipid levels and increased risk for the development of chronic diseases. However, some animal studies have demonstrated that dietary SFA may not raise blood lipid levels when the diet is sufficient in omega‐3 polyunsaturated fatty acids (n‐3PUFA). Therefore, in a randomised cross‐over design, we investigated the postprandial effects of feeding meals rich in either SFA (butter) or vegetable oil rich in omega‐6 polyunsaturated fatty acids (n‐6PUFA), in conjunction with n‐3PUFA, on blood lipid profiles [total cholesterol, low density lipoprotein cholesterol (LDL‐C), high density lipoprotein cholesterol (HDL‐C) and triacylglycerol (TAG)] and n‐3PUFA incorporation into plasma lipids over a 6‐h period. The incremental area under the curve for plasma cholesterol, LDL‐C, HDL‐C, TAG and n‐3PUFA levels over 6 h was similar in the n‐6PUFA compared to SFA group. The postprandial lipemic response to saturated fat is comparable to that of n‐6PUFA when consumed with n‐3PUFA; however, sex‐differences in response to dietary fat type are worthy of further attention. 相似文献
6.
High performance liquid chromatography with gel exclusion columns was used for quantitative measurement of plasma lipoproteins.
A combination of columns TKS 4000 PW and 3000 PW gave good separation of very low (VLDL), low (LDL) and high (HDL) density
lipoproteins. The area under each lipoprotein peak detected by absorbance at 280 nm was measured by digitizing and was expressed
as cm2. Purified lipoprotein standards isolated by ultracentrifugation were also chromatographed in increasing concentrations. The
area under the lipoprotein standard peak was linearly related to the amount of total protein over a wide range. The areas
of most of the measured plasma lipoproteins were within the linear range. The relationship between the area and the amount
of protein for each standard was used to quantitate the amount of protein and was expressed as mg/dl plasma. This technique
is simple and requires a small amount of plasma. The validated technique was applied to a large population of pedigreed baboons.
An average plasma lipoprotein profile of feral baboons on the chow diet was characterized by a high level of HDL (90.9±30.7
mg/dl) with a lesser amount of LDL (29.1±13.2 mg/dl). VLDL was present in much lower concentration (8.6±2.6 mg/dl). Feeding
a high cholesterol and high saturated fat (HCHF) diet raised both LDL (1.5-fold) and HDL levels (1.3-fold) without changing
VLDL levels. Progeny of sires with low response to dietary cholesterol increased their HDL protein when challenged with HCHF
diet without any change in their LDL or VLDL. Progeny of high-responding sires, however, had increases in both their HDL and
LDL levels when challenged with HCHF diet. The survey of lipoprotein profiles of the pedigreed baboon colony disclosed a number
of animals with interesting and unusual lipoprotein patterns. 相似文献
7.
The concentration and composition of the very low density lipoprotein (VLDL) lipids and the behavior of the VLDL in a density
gradient in the zonal ultracentrifuge were examined in plasma obtained from normal fed male and female rats before and after
intravenous injection of Triton WR-1339. Concentration of lipids in plasma VLDL of female rats was about half that of male
animals. Following injection with Triton WR-1339, the concentration of VLDL lipids was higher in female rats (triacylglycerol)
or similar (phospholipid, cholesterol, and cholesteryl esters) in both sexes. Female rats secreted much more VLDL triacylglycerol
into the plasma compartment than did the male animals under the same experimental conditions. No differences were observed
in lipid composition of the VLDL or in the position of the VLDL in the zonal rotor after ultracentrifugation in a density
gradient of the lipoprotein from plasma of normal male and female rats before treatment with the detergent. However, after
treatment with Triton, a higher proportion of the VLDL particles isolated from plasma of female rats displayed a more rapid
rate-zonal flotation in the ultracentrifuge than did the VLDL produced by the male. The VLDL secreted by female rats contained
fewer moles of phospholipid and free sterol per mol triacylglycerol than did the VLDL secreted by male animals under identical
experimental conditions. The molar ratio of free cholesterol: cholesteryl ester in the VLDL secreted after treatment with
Triton increased in both male and female rats. Simultaneously, the content of arachidonic acid in phospholipid of VLDL increased
with a concomitant decrease in cholesteryl ester. These changes in fatty acid composition suggest that the inhibitory effect
of Triton on lecithin-cholesterol acyl transferase activity affects the exchange of lipids between VLDL and high density lipoprotein.
It can be concluded from the data reported here that sex influences the concentration of plasma lipids in vivo and the output
and properties of the VLDL.
Presented in part at the 59th annual meeting of the Federation of American Societies for Experimental Biology, Atlantic City,
NJ, April 1975 (1).
Recipient of a Career Development Award from the U.S. Public Health Service, No. 1-K4-HL-70329. 相似文献
8.
Maryam S. Daneshpour Mehdi Hedayati Parisa Eshraghi Fereidoun Azizi 《European Journal of Lipid Science and Technology》2010,112(7):810-816
The prevalence of cardiovascular risk factors, especially low level of high density lipoprotein cholesterol (HDL‐C), is very high in Iran. The associations of apolipoprotein E (Apo E) polymorphism with lipid profile, especially high density lipoprotein (HDL) subfractions, were examined in Iranian population. A cross‐sectional study of 1030 subjects (452 men and 578 women) from the Tehran Lipid and Glucose Study (TLGS) was performed. Serum triacylglycerol, cholesterol, FBS, HDL‐C levels and its subfractions, Apo B and Apo A1 were determined and body mass index and blood pressure were measured. A segment of the mentioned gene was amplified by PCR and the polymorphisms were revealed by RFLP using HhaI restriction enzyme. Allele frequencies obtained for APOE*2, APOE*3, and APOE*4 were 5.77, 85.92, and 8.3%, respectively. The presence of the ε2 allele was significantly associated with increased serum HDL‐C levels and its subfractions both in men and women except HDL3 in men. The LDL/HDL ratio was significantly lower in female. The relations were significant even after adjustment for age, sex, and BMI but hypertension, smoking, and diabetes status decreased the effect of Apo E2 on HDL‐C and HDL subfractions. The observed genotype and allele frequencies were similar to those reported for other Caucasians samples. Apo E2 increased the level of HDL‐C, HDL subfractions, and decreased the LDL/HDL ratio. These findings highlight the important effect of variation in this gene on lipid levels. 相似文献
9.
Eicosapentaenoic acid is primarily responsible for hypotriglyceridemic effect of fish oil in humans 总被引:4,自引:0,他引:4
The aim of this study was to determine whether eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA), or both, were responsible
for the triglyceride (TG)-lowering effects of fish oil. EPA (91% pure) and DHA (83% pure), a fish oil concentrate (FOC; 41%
EPA and 23% DHA) and an olive oil (OO) placebo (all ethyl esters) were tested. A total of 49 normolipidemic subjects participated.
Each subject was given placebo for 2–3 wk and one of the n-3 supplements for 3 wk in randomized, blinded trials. The target
n-3 fatty acid (FA) intake was 3 g/day in all studies. Blood samples were drawn twice at the end of each supplementation phase
and analyzed for lipids, lipoproteins, and phospholipid FA composition. In all groups, the phospholipid FA composition changed
to reflect the n-3 FA given. On DHA supplementation, EPA levels increased to a small but significant extent, suggesting that
some retroconversion may have occurred. EPA supplementation did not raise DHA levels, however, FOC and EPA produced significant
decreases in both TG and very low density lipoprotein (VLDL) cholesterol (C) levels (P<0.01) and increases in low density lipoprotein (LDL) cholesterol levels (P<0.05). DHA supplementation did not affect cholesterol, triglyceride, VLDL, LDL, or high density lipoprotein (HDL) levels,
but it did cause a significant increase in the HDL2/HDL3 cholesterol ratio. We conclude that EPA appears to be primarily responsible for TG-lowering (and LDL-C raising) effects of
fish oil. 相似文献
10.
Vaijinath S. Kamanna Howard A. I. Newman Suman T. Patel Ahsok K. Tehim Donald T. Witiak Dennis R. Feller 《Lipids》1989,24(1):25-32
Influence of clofibrate and an aci-reductone, 4-(4-chlorophenyl)-2-hydroxytetronic acid (CHTA) on lipoproteins and apoproteins
was studied in cholesterol- plus cholic acid-fed rats. CHTA (0.4 mmol/kg body wt, twice daily) significantly lowered serum
total cholesterol and triglyceride concentrations at both 10 and 16 days, whereas clofibrate at the same dose did not alter
serum cholesterol levels, but elevated serum triglyceride concentrations at 16 days. The abnormal cholesterol-rich very low
density lipoproteins (VLDL), intermediate density lipoproteins (IDL) and low density lipoproteins (LDL) produced by cholesterol
plus cholic acid were significantly reduced in their cholesterol content by treatment with CHTA, a compound having an oxidation
reduction potential. Conversely, clofibrate administration increased VLDL-cholesterol with concomitant decreases in IDL- and
LDL-cholesterol concentrations. Administration of CHTA to cholesterol- plus cholic acid-fed rats significantly increased concentrations
of VLDL and IDL, but had no effect on HDL protein. Both CHTA and clofibrate administration to cholesterol- plus cholic acid-fed
rats significantly lowered IDL protein concentrations.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) studies of apoproteins revealed that clofibrate treatment
significantly reduced apoC-III and C-II in VLDL, C-II in IDL, and apoA-IV and A-I in HDL. Rats treated with CHTA significantly
raised apoC-II and C-III in HDL.
Isoelectric focusing (IEF) of VLDL apoproteins showed a significant decrease in apoC-II, C-III-0 and apoC-III-3 in clofibrate-treated
animals. Thus, the mechanism for antilipidemic action of the oxidation reduction compound, CHTA, which differs markedly from
the prototype drug, clofibrate, is independent from major apoprotein modification. 相似文献
11.
Willem F. Nieuwenhuizen Ilse Duivenvoorden Peter J. Voshol Patrick C. N. Rensen Wim van Duyvenvoorde Johannes A. Romijn Jef J. Emeis Louis M. Havekes 《European Journal of Lipid Science and Technology》2007,109(10):994-997
The prevalence of dyslipidemia and obesity resulting from excess energy intake and physical inactivity is increasing. The liver plays a pivotal role in the systemic lipid homeostasis. Effective, natural dietary interventions that lower plasma lipids and promote liver health are needed. APOE*3Leiden mice were fed a Western‐type diet, supplemented with different sphingolipids, to determine their effect on plasma lipids and liver steatosis. Hepatic lipid levels and lipid‐related gene expression were also determined. Dietary sphingolipids dose‐dependently lowered both plasma cholesterol (C) and triglycerides (TG) in APOE*3Leiden mice. 1% Phytosphingosine (PS) reduced C and TG by 57 and 58%, respectively. PS (a) decreased the absorption of dietary C and free fatty acid but did not affect the intestinal TG lipolysis, (b) increased hepatic VLDL‐TG production whereas plasma lipolysis was not affected; and (c) increased the hepatic uptake of VLDL remnants. Hepatic mRNA levels indicated enhanced hepatic lipid synthesis and VLDL and LDL uptake. Livers of PS (1%) fed mice were lighter (?22%), less pale, and contained less cholesteryl ester (?61%) and TG (?56%). Furthermore, markers for liver inflammation (SAA) and liver damage (ALAT) were decreased by 74% and 79%, respectively in PS‐fed mice. Sphingolipids lower plasma C and TG and protect the liver from fat‐ and cholesterol‐induced steatosis. In a preliminary small double‐blind cross‐over study with six middle‐aged slightly overweight male volunteers the daily supplementation of one gram of PS to the diet resulted in a ?9.8% (p = 0.0074) and – 13.2% (p = 0.0002) reduction of total C and LDL‐C, respectively. The C/HDL‐C ratio was not significantly affected (p = 0.0571). Due to the relatively low pre‐study levels of TG in the human volunteers, and the individual variability of TG levels, the TG lowering in humans was not significant in this first small study, but per individual there was a clear trend in TG lowering. 相似文献
12.
Pe¯teris Alberts Gunnel Klingstrm Vibeke Arrhenius‐Nyberg Catarina Larsson Kjell S. Sakariassen 《European Journal of Lipid Science and Technology》2006,108(6):453-458
The aim of the present study was to assess cholesterol‐containing lipoprotein profiles in minute serum samples. The lipoprotein profiles of KKAy and transgenic KKAy‐CETP mice and of other species were determined. The transgenic KKAy‐CETP mice express the simian enzyme cholesteryl ester transfer protein (CETP). The serum profile of the cholesterol‐containing high‐density (HDL), low‐density (LDL) and very‐low‐density lipoproteins (VLDL) was monitored on a Superose 6 column using fast protein liquid chromatography. Serum from several mouse and rat strains, rabbit, hamster, pig and man was included for comparative and method validation purposes. The chromatograms showed that the transgenic KKAy‐CETP mice had significantly decreased relative levels of HDL vs. VLDL and LDL cholesterol (p <0.001). Introduction of the CETP gene shifted the serum profile of the cholesterol‐containing lipoproteins of the KKAy‐CETP mice closer to the human profile in a dose‐dependent manner, thus making these mice an interesting model for man. The described lipoprotein separation technology offers promising and reliable opportunities for studies of blood lipoprotein profiles with minute serum samples, in both animals and man. 相似文献
13.
Arne T. Høstmark Øystein Spydevold Einar Lystad Eva Kristensen Ida Goffeng Bay 《Lipids》1982,17(7):489-499
The effect of varying the dietary sunflower oil/sucrose (SO/SU) ratio on rat plasma lipid concentration and lipoprotein distribution
was studied. Four groups of 10 rats were fed for 4 weeks diets with varying SO/SU ratios. Lipoprotein components were then
estimated in whole plasma and after cumulative density ultracentrifugation. Whole plasma triacylglycerol (TG), total cholesterol
(TC) and free cholesterol (FC) decreased with increasing SO/SU ratio; the CE/FC ratio increased, because CE remained virtually
unaltered. Plasma TG-lowering was due to a decrease in VLDL and LDL-TG. Protein, CE and FC in d=1.063–1.100 g/ml (HDL2b) and d=1.100–1.125 g/ml (HDL2a) lipoproteins decreased upon increasing the SO/SU ratio. In contrast, in d=1.125–1.200 g/ml (HDL3) lipoproteins, there was a concomitant increase in these components. Although increasing the SO/SU ratio effected more protein
and CE transportation in HDL3 and less in HDL2, the total amount of these components in high density lipoproteins (d=1.063–1.200 g/ml) remained constant. Apo A-I and apo
C-III decreased in HDL2 but increased in HDL3 upon increasing the SO/SU ratio. Also, HDL2 apo E, and the apo C-II/apo C-III and small apo B/large apo B ratios in VLDL and LDL were lowered by increasing the SO/SU
ratio. The hepatic VLDL-TG output during isolated liver perfusion was lowest in rats fed the diet with the highest SO/SU ratio.
In perfusate, like in plasma, the VLDL and LDL apo C-II/apo C-III ratio, as well as the small apo B/large apo B ratio, decreased
upon increasing the dietary SO/SU ratio. The results indicate that there can be appreciable diet-dependent variations in plasma
HDL subgroup distribution in spite of unchanged total HDL levels. 相似文献
14.
F. Abdullah M. Whiteford G. Mathiak P. Ovadia A. Rudolph L. F. Neville R. Rabinovici 《Lipids》1997,32(4):377-381
The present study investigated the effect of liposome-encapsulated hemoglobin (LEH), an experimental oxygen-carrying resuscitation
fluid, on triglyceride, total cholesterol, and low density lipoprotein (LDL), and high density lipoprotein (HDL) cholesterol
measurements. In vivo, the intravenous infusion of LEH (5.6 mL/kg, n=6) elevated serum triglycerides (+92% vs. baseline, P<.05), total cholesterol (+25% vs. baseline, P<.01), LDL cholesterol (+72% vs. baseline, P<.01) and had no effect on serum HDL cholesterol. In addition, LEH did not alter the elevation in serum triglycerides (+302%
vs. baseline, P<.01) and LDL cholesterol (+86% vs. baseline, P<.01) induced by lipopolysaccharide (3.6 mg/kg, i.v., n=6). Ex vivo, measurements of triglycerides and total cholesterol as well as LDL and HDL cholesterol in whole blood from naive rats were
not changed by the addition of LEH (0–50%, n=6). In vitro, the addition of a fixed concentration of LEH (50%, n=6) to varying concentrations of cholesterol solution (0–50%), or vice versa, had no effect on cholesterol determination. It is therefore concluded that LEH only minimally affects serum levels of triglycerides,
total cholesterol, LDL cholesterol, and HDL cholesterol and does not interfere with their measurement. 相似文献
15.
Native fish-eye disease plasma, which is deficient of both high density lipoproteins (HDL) and lecithin-cholesterol acyltransferase
activity (α-LCAT), processing the free cholesterol of these lipoproteins, has been supplemented with normal isolated HDL2 or HDL3 and incubated in vitro at 37 C. After incubation for 0,7.5 and 24 hr the very low density (VLDL) and low density (LDL) lipoproteins
as well as HDL were isolated, and their contents of triglycerides, phospholipids and free, esterified and total cholesterol
were quantified. The resulting net mass transfer of the different lipids revealed a functioning transfer of cholesteryl esters
and all other analyzed lipids between the lipoproteins, although no de novo esterification of the HDL cholesterol by LCAT
in this plasma occurred. In accordance with previous findings there was a functioning esterification process of the free cholesterol
of the combined VLDL and LDL of fish-eye disease plasma. The present results make it reasonable to conclude that the lack
of HDL cholesterol esterification in this disease is not a result of a deficiency of cholesteryl ester transfer or lipid transfer
activities. 相似文献
16.
17.
Soybean protein and casein supplemented with 1% Arg were compared for their ability to prevent fatty livers caused by excess
dietary Lys. The concentrations of serum lipids and lipoproteins of rats fed 5% Lys and having vatty livers were also compared
with those of rats fed the identical diet but lacking fatty livers when killed. The total liver lipids, triglycerides and
cholesterol of rats fed 15% casein +5% Lys were 3.9, 12.4 and 2 times control values, respectively. Rats fed 5% Lys +1% Arg
or 5% Lys with 15% soybean protein had liver lipid concentrations similar to controls fed no supplemental Lys. Serum total
lipids, triglycerides, cholesterol, phospholipids and free fatty acids also did not change, and serum ketone bodies were slightly
elevated with Lys feeding whether the rats had fatty livers or not. The concentrations of circulating HDL were slightly depressed
in all rats fed 5% Lys while LDL were significantly elevated, particularly in rats without fatty livers. Serum VLDL did not
change with 5% dietary Lys. Overall, excessive dietary Lys caused fatty livers which were prevented by varying the diet or
length of feeding. Excess Lys feeding altered lipoprotein metabolism shown by decreased serum HDL and a substantial elevation
in LDL. The latter was more apparent when the fat accumulation in liver was less severe or absent. The data suggest that the
fatty liver from Lys excess is probably unrelated to increased fat mobilization from storage, decreased fat oxidation or to
a major block in the transport of triglycerides from the liver to the circulation. 相似文献
18.
Composition and concentration of lipoproteins in the serum of normal rats and rats deficient in essential fatty acids 总被引:2,自引:0,他引:2
A comparison is made of the concentration and chemical composition of serum lipoproteins of normal rats and rats deficient
in essential fatty acids. The concentration of very low density lipoproteins (VLDL) and of low density lipoproteins (LDL)
in serum of deficient rats is about half that found in normal rats, but the concentration of high density lipoproteins (HDL)
is higher than normal and they contain an increased amount of cholesterol esters. The proportion of cholesterol that is esterified
is much greater than normal in the serum of deficient rats. The deficiency of essential fatty acids also appears to result
in compensating changes occurring in the composition of serum lipoproteins. In both VLDL and LDL of deficient rats the proportion
of protein is raised and that of phospholipid lowered compared to normal, while the proportions of trigly ceride and cholesterol
esters are unchanged. 相似文献
19.
Effect of chronic glucagon administration on lipoprotein composition in normally fed,fasted and cholesterol-fed rats 总被引:2,自引:0,他引:2
Catherine Guettet Najmuddin Rostaqui Denis Mathe Bernard Lecuyer Nicole Navarro Bernard Jacotot 《Lipids》1991,26(6):451-458
Male adult Wistar rats received daily (at 9 a.m. and 5 p.m.) 10 μg of zinc-protamine glucagon by subcutaneous injection for
8 days. Plasma cholesterol levels were decreased by 36% in fed rats, 33% in cholesterol-fed rats and by 55% in fasted rats.
Lipoproteins were separated into 22 fractions by ultracentrifugation using a density gradient. Glucagon administration decreased
the cholesterol content in all lipoproteins except low density lipoprotein (LDL1) (1.006–1.040) and very low density lipoprotein (VLDL) from cholesterol-fed rats. The main decrease (−57 to −81%) was observed
in 1.050–1.100 g/mL lipoproteins (LDL2 and HDL2), which contained a large amount of apo E, while HDL3 cholesterol was not affected. Triacylglycerol levels were decreased only in chylomicrons and VLDL (−70%) of fed and cholesterol-fed
rats, while plasma and lipoprotein triacylglycerol levels were not changed in fasted rats treated with glucagon. In normally
fed rats glucagon administration increased by 42% the fractional catabolic rate of [125I]HDL2 while the absolute catabolic rate appeared to be unchanged. Glucagon seems to be a potent hypolipidemic agent affecting mainly
the apo E-rich lipoproteins. Its chronic administration limits lipoprotein accumulation which occurs upon cholesterol feeding. 相似文献
20.
Changes in serum lipoprotein profiles were characterized in Donryu rats subcutaneously implanted with an ascites hepatoma
line of AH109A cells and the effect of a new hypolipidemic agent with a structure of 4-(4′-chlorobenzyloxy)benzyl nicotinate
(KCD-232) was estimated. With the growth of hepatoma for periods of up to three weeks, a striking decrease in the high density
lipoprotein (HDL) fraction and an enormous increase in the VLDL+LDL (very low density lipoprotein+low density lipoprotein)
fraction were found in hepatoma-bearing rats when either precipitation method or electrophoresis was used. These lipoprotein
profiles were not influenced by sex. Judging from the electrophoretogram, the increase in VLDL+LDL fraction was due mainly
to an increase in LDL fraction. The oral administration of KCD-232 significantly suppressed the hepatoma-induced increase
in VLDL+LDL with little or no influence on the hepatoma-induced decrease in HDL. There existed a positive correlation between
the hepatoma weight and (VLDL+LDL)-cholesterol concentration and a negative one between hepatoma and HDL-cholesterol. 相似文献