Studies on baking of high alpha-amylase flours: effect of pH,salt and L-cysteine . HCI in the dough

The effect of varying concentrations of sodium chloride (0 to 2.5%), pH (5.8 to 4.2) and KBrO₃ (75 ppm) with and without addition of L-cysteine ± HCI (25 to 75 ppm) on the baking of flours with high content of alphaamylase (108 to 540 SKB/100 g) was compared with a normal flour. Decreasing the pH of dough to 4.2 and increasing the salt concentration to 2.5% reduced the loaf volume and gluten recoveries considerably. Decreased pH also reduced the mixing tolerance of dough, which was counteracted by increased salt content of normal and high alpha-amylase flours. A combination of low pH, salt and L-cysteine ± HCI and 75 ppm of KBrO₃ without sugar in the dough of higher alpha-amylase flour produced a satisfactory loaf by eliminating bulk fermentation.     

Effect of Amino Acids and Peptides on Mixing and Frozen Dough Properties of Wheat Flour

Free amino acids, peptides, and vital wheat gluten were investigated to determine their effect on the mixing and frozen dough baking properties of wheat flour. Addition of 1% cysteine and aspartic acid decreased and glutamic acid, histidine, arginine, and lysine increased the mixing tolerance of flour. Cystine, methionine, tryptophan, and phenylalanine increased but isoleucine, histidine, glycine, arginine, glutamic acid, aspartic acid, and lysine decreased loaf volume of nonfrozen dough breads. However cystine, methionine, tryptophan, and phenylalanine did not increase loaf volume of bread prepared from frozen dough. Vital wheat gluten increased mixing tolerance and bread loaf volume only for the nonfrozen dough. However, wheat gluten hydrolysate, corn, and bonito peptides decreased mixing tolerance after optimum mixing time and were effective in increasing loaf volume for both frozen and nonfrozen dough. As the amount of corn and bonito peptide increased, specific loaf volumes also increased. Addition of 2.5% corn peptide was most effective in increasing loaf volume of frozen dough bread. Crust browning and crumb stickiness increased, whereas crumb softness decreased with addition of peptides. Addition of less than 1% peptide did not adversely affect the aftertaste and off‐flavor of bread. These results suggest that addition of peptides are effective for improving the baking quality of frozen dough, whereas amino acids and gluten have no effect.     

Short-Time Breadmaking Systems. Effect on Oxidation

A spread test was used to study the effects of fermentation time and various agents on dough. Short fermentation times (15–30 min) greatly improved spread ratio. Yeast also improved spread ratio, particularly in combination with time. A combination of 60 ppm KBrO₃ and 100 ppm ascorbic acid was the most effective oxidant system. There was an obvious interaction between yeast and oxidants. Loaf volume was not affected by the timing of addition of yeast or ascorbic acid to dough. Potassium bromate was more effective when added at the premix. A 60 min rest between premix and remix consistently gave higher loaf volume when yeast was added, but not when both yeast and oxidants were present.     

EFFECTS OF GLUCOSE OXIDASE, HEMICELLULASE AND ASCORBIC ACID ON DOUGH AND BREAD QUALITY

ABSTRACT

In this study, glucose oxidase alone or its combinations with hemicellulase or ascorbic acid were used in bread making. Glucose oxidase alone mainly decreased dough extensibility. It produced stiffer and less extensible dough. Combinations of glucose oxidase–hemicellulase presented lower extensibility and were more resistant to extension than glucose oxidase alone. When glucose oxidase–ascorbic acid combinations were used, the softening degree significantly decreased, regardless when added the lowest glucose oxidase in combination with ascorbic acid. Glucose oxidase–ascorbic acid combinations significantly modified dough resistance. The glucose oxidase alone significantly increased specific loaf volume. The Dallman value of loaves made with glucose oxidase alone was found higher than for control. The most dramatic effect of additives on specific loaf volume was observed when glucose oxidase–hemicellulase combinations were added. This effect has been ascribed to redistribution of water from the hemicellulose to gluten, which would render the gluten more extensible. Specific loaf volume showed a significant enhancement when glucose oxidase–ascorbic acid combinations were added, but this effect was not as good as glucose oxidase–hemicellulase. The effects of glucose oxidase and its combinations with ascorbic acid and hemicellulase on dough rheology and bread quality are highly dependent on the amount of enzyme and the original wheat flour quality.

PRACTICAL APPLICATION

In practice, appropriate combinations of glucose oxidase with hemicellulase can be used as improvers in bread making, depending on their combination levels. This study will show the way to new research about glucose oxidase, ascorbic acid and hemicellulose.

     

Short-time Breadmaking Systems. Effect of Formulation, Additives, Temperature, and Flour Quality

An optimum formula for short-time breadmaking included flour (100%), yeast (5%), sugar (3%), salt (1.5%), shortening (3%), oxidant (100 ppm ascorbic acid and 60 ppm KBrO₃), and optimum water. The minimum fermentation time for good volume was 15 min. Ambient temperature greatly affected baking performance, and formula changes would be required to produce optimum short-time bread at different temperatures. Baking the same flours by both short- and long-time baking methods gave poor correlations between loaf volumes and oxidant requirements.     

Use of Enzymes to Minimize Dough Freezing Damage

The purpose of this investigation was to study the effect of pentosanase (Pn), glucose oxidase (Gox), and transglutaminase (TG) on frozen dough (−18 °C) and their influence on minimizing the damage caused by frozen storage. Bread characteristics were analyzed on day 0; after 3 and 9 weeks of frozen storage, specific loaf volume, crust color, and crumb texture and structure were analyzed. Dough expansion capacity and dough stickiness, extensibility, and viscoelasticity were determined. Frozen dough with high levels of Gox developed a larger bread volume than control dough (without added enzyme). The damage percentage caused by frozen storage in Gox samples was lower than in control samples, indicating that Gox increased dough strength and counteracted the depolymerization effect of gluten produced by ice crystal formation and the release of reducing substances from dead yeast cells during freezing. Samples with Pn developed a large bread volume after 9 weeks of frozen storage because of the formation of smaller pentosans, which result from Pn enzyme action. These pentosans were located in protein–starch–CO₂ matrix interfaces and increased dough expansion capacity without gas loss, thus allowing a higher expansion during proofing. The intermediate level of TG was the only one to present a larger bread volume from frozen dough than control. The new isopeptidic bonds introduced by TG in the gluten proteins helped to mitigate the damage caused by dough freezing.     

Hearth bread baking quality of durum wheat varying in protein composition and physical dough properties

Thirty durum wheat genotypes from ten countries of origin were grown in field plots for two consecutive years. Three of the genotypes were γ‐gliadin 42 types and the remainder were γ‐gliadin 45 types. Among the γ‐gliadin 45 types, six high‐molecular‐weight glutenin subunit (HMW‐GS) patterns were identified: 6 + 8, 7 + 8, 7 + 16, 14 + 15, 20 and 2*, 20. All the γ‐gliadin 42 genotypes contained low amounts of unextractable polymeric protein (UPP) and exhibited low gluten index values and weak gluten properties. The γ‐gliadin 45 genotypes exhibited a wide range of UPP, gluten index and dough strength. HMW‐GS 20 genotypes were generally weak, whereas HMW‐GS 6 + 8 and 7 + 8 genotypes were generally strong. When baked by a lean formulation, long‐fermentation straight‐dough hearth bread process, the durum wheat genotypes exhibited a wide range of baking quality. Loaf volume and bread attributes were strongly correlated with UPP and gluten index. Some of the genotypes exhibited bread attributes and loaf volume equal or slightly superior to those of a high‐quality bread wheat flour. However, even the strongest durum wheat genotypes exhibited inferior fermentation tolerance to the bread wheat flour, as seen by a requirement for lower baking absorption during dough handling and more fragile dough properties when entering the oven. Among the HMW‐GS groups, HMW‐GS 7 + 8 and 6 + 8 exhibited the best and HMW‐GS 20 the poorest baking quality. Farinograph, alveograph and small‐scale extensigraph properties demonstrated that a combination of dough elasticity and extensibility was needed for superior durum wheat baking performance. Copyright © 2007 Society of Chemical Industry     

Improvers and functional ingredients in whole wheat bread: A review of their effects on dough properties and bread quality

BackgroundDespite the associated health benefits of whole grains, consumption of whole grain products remains far below recommended levels. Whole wheat bread is often associated with many distinctive attributes such as low loaf volume, firm and gritty texture, dark and rough crust and crumb appearance, bitter flavor, and reduced shelf-life. There is a need to improve its quality and sensory characteristics so as to increase consumer appeal and, ultimately, increase the intake of whole wheat bread. The inclusion of various ingredients improves dough and bread properties.Scope and approachThis review examines the effects of enzymes, emulsifiers, hydrocolloids, and oxidants on the properties of whole wheat bread and dough, with particular attention to effects on loaf volume and hardness. Wheat gluten and other plant materials are also discussed. Gaps in the research into whole wheat bread are identified, and future research needs are recommended.Key findings and conclusionsXylanase reduces the water absorption of whole wheat flour and increases loaf volume and crumb softness by hydrolyzing ararbinoxylans. α-amylase can be beneficial under certain conditions. Phytase may activate endogenous α-amylase. G4-amylase is promising but needs validation by further research on its effect on loaf volume, crumb hardness, and staling. Vital wheat gluten overcomes many of the challenges of whole wheat bread production and is found in the majority of commercial whole wheat breads. Emulsifiers DATEM and SSL can improve the volume, texture and staling profile of whole wheat bread. Several types of improvers are generally needed in combination to provide the greatest improvement to whole wheat dough and bread.     

Comparative studies on physicochemical and baking properties of newly harvested and stored indian varieties of wheat

Comparative studies on the progressive changes in distribution pattern of proteins, and in rheological and baking properties, were carried out with seven newly harvested and stored wheat varieties. Freshly harvested wheat contained a larger amount of low molecular weight gliadins, which were aggregated during storage in air. This resulted in improvement of the rheological and baking properties of stored grains. Thus, gluten content in wheat or, more precisely, the interchange reactions between thiol and disulphide during storage, governed the dough rheology. The same effects could be achieved by addition of an oxidising agent such as KBrO₃. Radiation treatment (up to 200 krad) also improved the baking quality of newly harvested wheat by modifying some of its rheological properties. A significant correlation between SS/SH ratio and loaf volume was observed. Lower loaf volume in newly harvested wheat was associated with its low SS/SH ratio, high maximum gelatinisation viscosity and low gas retention capacity.     

Effect of phenolic acids on the rheological properties and proteins of hard wheat flour dough and bread

BACKGROUND: The effects of different phenolic acids on the rheological properties and gluten proteins of hard wheat flour dough and bread were investigated. Caffeic, ferulic, syringic and gallic acids were each blended with hard wheat flour at a concentration of 4.44 µmol L^?1 g^?1 flour. RESULTS: Mixing time and tolerance were reduced with the addition of phenolic acids. The phenolic acids reduced the maximum resistance to extension (R_max) and increased the extensibility of dough, with effects in the following order: gallic < syringic < ferulic < caffeic acid. The effect on R_max was more pronounced in overmixed dough. Loaf volume was most significantly decreased with the addition of caffeic acid. Extraction of sodium dodecyl sulfate‐soluble high‐molecular‐weight proteins was increased in both mixed and fermented doughs by the addition of ferulic and caffeic acids. The order of influence of the phenolic acids on the rheological properties and protein structure of dough and bread was consistent with that of their antioxidant activity. CONCLUSION: The addition of caffeic and ferulic acids reduced R_max and increased the extensibility of hard wheat flour dough by modifying the high‐molecular‐weight gluten, which resulted in decreased bread volume. Copyright © 2011 Society of Chemical Industry     

Rheological properties of sheeted wheat flour dough measured with large deformations

Abstract

Large deformation rheological properties of a bread dough sheet were compared with baking quality of dough from the same sheet. Both rheological test and baking test could differentiate between dough made from a strong and a weak flour. Apparent viscosity at large deformation appeared to be the best predictor of the minimum sheeting requirement to obtain high loaf volume. This is similar to Mechanical Dough Development (MDD) mixing of dough where the mixing curve (apparent viscosity) is used to determine mixing requirement. Baking tests showed that loaf quality (volume and crumb texture) for bread made with sheeting was different from bread made with MDD mixing, particularly when dough was developed beyond the minimum sheeting requirement. These differences indicate that dough development (or over‐development) by sheeting may occur through a different mechanism than by MDD mixing.     

Fermentation Process and Grain Structure of Baked Breads from Frozen Dough Using Freeze-Tolerant Yeasts

The fermentation process for frozen doughs using freeze‐sensitive (Saccharomyces cerevisiae, Kyowa for sweet bread; S. cerevisiae, FC for white bread) and freeze‐tolerant (S. cerevisiae, YF for sweet bread) yeasts was traced by magnetic resonance imaging (MRI). Grain network structures of baked breads were also visualized by MRI. Prefermentation before freezing, punching and remolding, or resheeting and molding treatments increased loaf volume by 10 to 110% for the baked breads using freeze‐tolerant yeast, while these treatments decreased loaf volume by 70% using freeze‐sensitive yeast. The first fermentation before freezing and the second fermentation with punching or resheeting after thawing are useful for obtaining good quality breads from frozen dough using freeze‐tolerant yeast.     

Impact of Green Tea Extract and Fungal Alpha-Amylase on Dough Proofing and Steaming

Green tea extract (GTE) was fortified into steamed bread as a functional ingredient to enhance its nutritional values. However, GTE might inhibit α-amylase activity and interact with gluten proteins, causing adverse effects on dough development and final loaf volume. This research investigated the effects of GTE and fungal alpha-amylase (FAA) on rheofermentometer characteristics, dough inflation parameters, and the specific volume of both dough and steamed bread. Rheofermentograph showed that the fortification of GTE did not affect the gassing power of yeast while it slightly inhibited the activity of FAA. Fortification of GTE at the level of 1.0 % decreased the dough inflation parameters and the specific volume of steamed bread. On the other hand, fortification of 60 ppm FAA enhanced the dough inflation parameters and increased the specific volume of steamed bread. Addition of 60 ppm FAA was able to fully compensate for the reduction of specific volume caused by the addition of 1.0 % GTE. Fortification of 0.50 % GTE produced steamed bread whose specific volume was not significantly different from that without GTE.     

Combined effects of freezing rate, storage temperature and time on bread dough and baking properties

This study compares the effects of freezing temperature and rate as well as storage temperature and time on the quality of frozen dough. Yeasted bread dough was frozen using four freezing rates (19-69 °C/h), then stored at −10, −20, −30, or −35 °C for up to 180 days. Dough strength diminished with longer storage time and higher storage temperatures. Cryo-SEM showed that dough stored at −30 and −35 °C had the least damaged gluten network. NMR studies showed that more rapidly frozen dough, and that stored at lower temperatures had lower transverse relaxation (T₂) times (9-10 ms). However, dough stored at −20 °C displayed the highest yeast activity among samples. Bread loaf volume decreased with storage time, and bread made from dough stored at −20 °C showed the highest loaf volume. Breads produced from −30 and −35 °C stored dough displayed less change in the texture profile during storage as well as less change in T₂ values. Response surface analysis showed that optimal properties occurred at freezing rates of around 19-41 °C/h and storage temperatures of −15 to −20 °C.     

Cryoprotective Role of Exogenous Trehalose in Frozen Dough Products

Aim of this work is to study the cryoprotective role of extracellular trehalose in the production of bakery products from frozen dough. Therefore, different levels of trehalose (up to 200 ppm) were incorporated in dough/bread samples made from white and whole-wheat flour, and their quality (loaf volume, weight loss during baking, crust and crumb color) and texture characteristics (dough, crust and crumb firmness) were examined during frozen storage. To investigate the role of trehalose on dough behavior, the sugar content (glucose, fructose, and sucrose) of dough samples composed with or without trehalose was monitored, and dough microstructure was also analyzed with scanning electron microscopy. The cryoprotective effect of trehalose was confirmed, and it was found proportional to its level for both flour types. Trehalose can improve dough behavior under freezing conditions in terms of bread volume and texture characteristics.     

Evaluation of viscoelastic properties and air-bubble structure of bread containing gelatinized rice

The impact of addition of gelatinized rice porridge to bread has been investigated on loaf volume, viscoelastic properties and air-bubble structure. We prepared four variety of bread: bread containing rice porridge (rice porridge bread), bread containing gelatinized rice flour (gelatinized rice flour bread), and wheat flour and rice flour breads for references. Instrumental analyses the bread samples were carried out by volume measurement of loaf samples, creep test and digital image analysis of crumb samples. Rice porridge bread showed the maximum specific volume of 4.51 cm³/g, and even gelatinized rice flour bread showed 4.30 cm³/g, which was larger than the reference bread samples (wheat and rice flour breads). The values of viscoelastic moduli of gelatinized rice flour bread and rice porridge bread were significantly smaller (p < 0.05) than those of wheat flour and rice flour breads, which indicates addition of gelatinized rice flour or rice porridge to bread dough encouraged breads softer. Bubble parameters such as mean air- bubble area, number of air-bubble, air-bubble area ratio (ratio of bubble area to whole area) were not significantly different among the bread crumb samples. Therefore, the bubble structures of the bread samples seemed to similar, which implied that difference of viscoelasticity was attributed to air-bubble wall (solid phase of bread crumb) rather than air-bubble. This study showed that addition of gelatinized rice to bread dough makes the bread with larger loaf volume and soft texture without additional agents such as gluten.     

木聚糖酶对冷冻面团和馒头品质的影响

本实验研究了木聚糖酶对冻藏1d、7d、14d的冷冻面团和馒头品质特性的影响;并采用差示量热扫描仪(DSC)测定了冷冻面团的可冻结水(冰)含量,讨论了木聚糖酶改善冷冻面团品质的热力学机制。研究结果表明,随着冻藏时间的延长,面团的发酵力、酵母存活率、馒头比容、抗老化能力均呈现一定的下降趋势;在相同的冻藏时间下,木聚糖酶对面团的发酵特性和馒头品质改善作用明显;木聚糖酶含量为80mg.kg_-1时,可冻结水(冰)含量最低;添加木聚糖酶后,冰晶颗粒更加细小均匀。这说明适量的木聚糖酶能够有效地降解水不溶性阿拉伯木聚糖,使更多的水分保留在面筋网络结构中,从而抑制低温面筋网络结构和酵母细胞的破坏作用,改善馒头品质。     

Experiments on dough rheology to improve screening of bread wheat cultivars

The bread‐making potential of flour may be roughly estimated by dough rheology, especially its tolerance to over‐mixing as determined with the farinograph. The objective of this study was to identify the relative effects of experimental conditions likely to affect dough mixing stability: mixer speed, temperature, salt, yeast and bread additives such as ascorbic acid and preservatives. The addition of 1–2% salt or ascorbic acid (50 mg kg^?1 flour) improved dough mixing stability and counteracted the negative effect of bread preservatives. Mixing salted dough at slow speed (63 rpm) and 25 °C might be a more realistic bread‐making procedure for performing dough rheology assays with equipment such as the farinograph, compared to official methods (only flour and water, no salt; 30 °C). Amongst five bread wheat cultivars, differences existed in dough strengthening response to both salt and ascorbic acid, a property that may find application in wheat breeding and screening.     

Evaluation of baking procedures for incorporation of barley roller milling fractions containing high levels of dietary fibre into bread

BACKGROUND: Roller milling of hull‐less barley generates fibre‐rich fractions (FRF) enriched in non‐starch polysaccharides from the endosperm cell walls (β‐glucans and arabinoxylans). This investigation was initiated to compare the suitability of different baking processes and to determine the optimal conditions for incorporation of barley FRF into pan bread. RESULTS: Addition of FRF from waxy and high‐amylose starch hull‐less barley genotypes was evaluated in pan bread prepared from Canada Western Red Spring (CWRS) and Canada Western Extra Strong (CWES) wheat flour. Three bread processes were used: Canadian short process (CSP), remix‐to‐peak, and sponge‐and‐dough. Addition of 20% FRF (equivalent to enrichment with 4.0 g of arabinoxylans and β‐glucans per 100 g of flour) disrupted dough properties and depressed loaf volume. CSP was not suitable for making FRF‐enriched bread because dough could not be properly developed. FRF‐enriched remix‐to‐peak bread was better, especially for the stronger CWES flour. The better bread quality compared to CSP was probably due to redistribution of water from non‐starch polysaccharides to gluten during fermentation prior to remixing and final proof. The sponge‐and‐dough process produced the best FRF‐enriched bread because of the positive effect of sponge fermentation on gluten development and hydration. FRF was added at the dough stage to fully developed dough. CONCLUSION: The method of bread production strongly influences bread quality. Pre‐hydration of FRF improved bread quality. CWRS and CWES flour produced comparable FRF‐enriched sponge‐and‐dough bread. Addition of xylanase to the sponge‐and‐dough formula improved the loaf volume, appearance, crumb structure and firmness of FRF‐enriched bread. Copyright © 2007 Society of Chemical Industry     

D-Erythroascorbic Acid in Bakers'Yeast and Effects on Wheat Dough

Extraction of hydrated and freeze-dried bakers’yeast yielded ～ 50 μg erythroascorbic acid (EAA) and 5 μg ascorbic acid (AA)/g dry yeast as determined by HPLC with electrochemical detection. D-EAA (82 ppm based on flour) slightly increased the flow of dough as rest time increased. Gluten isolated from a flour-water dough containing 82 ppm D-EAA or 100 ppm Lcysteine stretched at a faster rate than control gluten. EAA like AA did not change dough development time. Unlike AA, EAA showed neither oxidizing effect on dough and gluten nor improving effects on bread.