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Julietta Jablasone Lubov Y Brovko Mansel W Griffiths 《Journal of the science of food and agriculture》2004,84(3):287-289
The transmission of Salmonella Enteritidis from soil to fruit by contaminated irrigated water was studied using 20 patio tomato plants. In order to track the presence of Salmonella in the soil and plants a luminescent strain transformed with the full luxCDABE gene cassette from Photorhabdus luminescens was used. The tomato plants were irrigated every other day by direct application of water containing Salmonella Enteritidis (105 CFU ml?1) to the soil. Samples of soil, stem, leaf and fruit were taken weekly and assayed for Salmonella by plating onto Luria Bertani agar containing 50 µg ml?1 ampicillin. There was a significant difference (P < 0.05) in Salmonella counts from soils sampled during the course of the study. No Salmonella were recovered from the leaf, stem, and fruit samples taken from the tomato plants. This indicates that, under these test conditions, watering with contaminated water directly into the soil does not result in the transmission of Salmonella, and possibly other pathogens, to tomatoes. Copyright © 2004 Society of Chemical Industry 相似文献
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The optimal conditions of washing iceberg lettuce with acidified warm water to improve the hygienic and sensory product quality were determined with the aid of a central composite design. To determine the criteria of product quality, in the course of storage for 13 days at 4 °C lettuce was subjected every 2 days to sensory evaluation. The sensory values were found to correlate with the physical measurements of color (a* value) (R2=0.83) and texture (specific energy of deformation) (R2=0.77), respectively. Color, texture and overall visual quality correlated with product acceptability (R2=0.96, 0.97, 0.96, respectively). In the experiments of series I, the effect of temperature at washing (45–50 °C), pH (4–5) and treatment time (5–10 min) were investigated and the results were used for the construction of a model to describe the effects on color and texture and to define an area of sensory product acceptance. In series II the parameters were changed to ranges of temperature (45–50 °C), pH (4.6–6) and treatment time (2–5 min), and the effect on the reduction of bacterial counts within the area of product acceptance was defined. In that area reduction rates of total counts by 0.9–2.9 log cfu/g and Enterobacteriaceae by 0.8–3.0 log cfu/g were achieved. The initial germ- reducing effect was sustained until the end of storage for 7 days at 4 °C. At 50 °C, washing for 5 min at pH 4.93, the total bacteria and Enterobacteriaceae were reduced by 2.9 and 3.7 log cfu/g, respectively. The removal of the two groups of bacteria exhibited characteristic differences. Two independent experiments confirmed the reliability of the models. 相似文献