共查询到20条相似文献，搜索用时 15 毫秒
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John H. Slater Jordan S. Miller Shann S. Yu Jennifer L. West 《Advanced functional materials》2011,21(15):2798-2798
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Rabea Link;Kai Weißenbruch;Motomu Tanaka;Martin Bastmeyer;Ulrich S. Schwarz; 《Advanced functional materials》2024,34(20):2302145
With the advent of mechanobiology, cell shape and forces have emerged as essential elements of cell behavior and fate, in addition to biochemical factors such as growth factors. Cell shape and forces are intrinsically linked to the physical properties of the environment. Extracellular stiffness guides migration of single cells and collectives as well as differentiation and developmental processes. In confined environments, cell division patterns are altered, cell death or extrusion might be initiated, and other modes of cell migration become possible. Tools from materials science such as adhesive micropatterning of soft elastic substrates or direct laser writing of 3D scaffolds have been established to control and quantify cell shape and forces in structured environments. Herein, a review is given on recent experimental and modeling advances in this field, which currently moves from single cells to cell collectives and tissue. A very exciting avenue is the combination of organoids with structured environments, because this will allow one to achieve organotypic function in a controlled setting well suited for long-term and high-throughput culture. 相似文献
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A novel method for the fabrication and the use of simple uniform poly(dimethylsiloxane) PDMS substrates for controlling cell motility by a mechanical gradient is reported. The substrate is fabricated in PDMS using soft lithography and consists of a soft membrane suspended on top of a patterned PDMS substrate. The difference in the gradient stiffness is related to the underlying pattern. It is shown experimentally that these uniform substrates can modulate the response of cell motility, thus enabling patterning on the surfaces with precise cell motility. Because of the uniformity of the substrate, cells can spread equally and a directional movement to stiffer regions is clearly observed. Varying the geometry underlying the membrane, cell patterning and movement can be quantitatively characterized. This procedure is capable of controlling cell motility with high fidelity over large substrate areas. The most significant advance embodied in this method is that it offers the use of mechanical features to control cell adhesion and not topographical or chemical variations, which has not been reported so far. This modulation of the response of cell motility will be useful for the design and fabrication of advanced planar and 3D biological assemblies suitable for applications in the field of biotechnology and for tissue‐engineering purposes. 相似文献
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Greg M. Harris Maria E. Piroli Ehsan Jabbarzadeh 《Advanced functional materials》2014,24(16):2396-2403
A wide variety of environmental factors including physical and biochemical signals are responsible for stem cell behavior and function. In particular, matrix elasticity and cell shape have been shown to determine stem cell function, yet little is known about the interplay between how these physical cues control cell differentiation. For the first time, by using ultraviolet (UV) lithography to pattern poly(ethylene) glycol (PEG) hydrogels, it is possible to manufacture microenvironments capable of parsing the effects of matrix elasticity, cell shape, and cell size in order to explore the relationship between matrix elasticity and cell shape in mesenchymal stem cell (MSC) lineage commitment. These data show that cells cultured on 1000 μm2 circles, squares, and rectangles are primarily adipogenic lineage regardless of matrix elasticity, while cells cultured on 2500 and 5000 μm2 shapes more heavily depend on shape and elasticity for lineage specification. It is further characterized how modifying the cell cytoskeleton through pharmacological inhibitors can modify cell behavior. By showing MSC lineage commitment relationships due to physical signals, this study highlights the importance of cell shape and matrix elasticity in further understanding stem cell behavior for future tissue engineering strategies. 相似文献
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Katrin A. Barth Géraldine Coullerez Lina M. Nilsson Riccardo Castelli Peter H. Seeberger Viola Vogel Marcus Textor 《Advanced functional materials》2008,18(9):1459-1469
The study of the adhesion mechanisms of pathogens to host tissues has gained increased interest as bacterial adhesion is involved in the early stages of surface colonization and infection. Here we describe a platform to study the specific binding of the bacterium Escherichia coli (E. coli) K‐12 strain to molecularly well‐defined surfaces mimicking cellular interfaces. This approach uses a poly(ethylene glycol) brush interface, which displays synthetic determinants of the high mannose N‐linked glycans in a range of densities (3.8 × 104–1.6 × 105 mannosides µm?2) for the investigation of multivalent interactions with bacteria. The bacterial attachment is mediated by specific interactions between the adhesive protein FimH located on the tip of the bacterial type 1 pili and the mannosylated surfaces. With synthetically engineered mannoses, it is found that the number of strongly adhering bacteria is co‐regulated by many structural physical parameters. Beyond the dependency on carbohydrate density, higher numbers of E. coli attach to the branched trimannose Man(α1–3)(Man(α1–6))Man compared to the monomannose, while larger oligomannoses exposing Man(α1–2) Man at their non reducing end show low binding capacity. The linker used between the mannose moiety and PEG is also affecting the binding efficacy of E. coli. The (hydrophobic) propyl linker results in higher bacteria numbers in comparison to the (hydrophilic) tri(EG), likely a consequence of additional stabilization of the binding complex by hydrophobic interactions. Furthermore, differences are observed in bacteria attachment between stagnant and flow conditions that depend on the type of mannose ligand. Finally, a photolithographic resist lift‐off combined with site‐selective assembly of the glycopolymers is used to produce micropatterns with bacteria colonies confined to defined areas and at controlled colony numbers. 相似文献
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无机激光热刻蚀材料的研究进展 总被引：1，自引：0，他引：1
激光热刻蚀技术是近年发展起来的利用激光热刻蚀薄膜的热变化阈值特性突破光学衍射极限进行纳米图形制备的新技术,在亚波长纳米结构器件和高密度光盘母盘制造等领域具有广阔的应用前景.阐述了激光热刻蚀技术的基本原理和特点,以及对激光热刻蚀材料性质的要求,综述了相变型激光热刻蚀薄膜材料(包括硫系相变薄膜材料、金属亚氧化物薄膜材料和陶... 相似文献
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本文针对无铬掩模方式，从计算机摸拟和实验两个方面，研究了ＫｒＦ准分子激光（λ＝２４８ｎｍ）接触式移相曝光。得到了０．１μｍ的正胶线条。得出了在接触式相干平行光照射下，移相器与基片间距对光刻线条宽窄有很大影响的结论，并通过将移相器直接做在基片上，给出了移相器紧贴基片的可靠方法。 相似文献
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本文综述了准分子激光光刻的发展,着重讨论准分子激光光刻的优点及其在提高分辨率方面的进展,并讨论了今后的动向。 相似文献
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Tobias Ekblad Lars Faxälv Olof Andersson Nanny Wallmark Andréas Larsson Tomas L. Lindahl Bo Liedberg 《Advanced functional materials》2010,20(15):2396-2403
This work describes the preparation and properties of hydrogel surface chemistries enabling controlled and well‐defined cell adhesion. The hydrogels may be prepared directly on plastic substrates, such as polystyrene slides or dishes, using a quick and experimentally simple photopolymerization process, compatible with photolithographic and microfluidic patterning methods. The intended application for these materials is as substrates for diagnostic cell adhesion assays, particularly for the analysis of human platelet function. The non‐specific adsorption of fibrinogen, a platelet adhesion promoting protein, is shown to be completely inhibited by the hydrogel, provided that the film thickness is sufficient (>5 nm). This allows the hydrogel to be used as a matrix for presenting selected bioactive ligands without risking interference from non‐specifically adsorbed platelet adhesion factors, even in undiluted whole blood and blood plasma. This concept is demonstrated by preparing patterns of proteins on hydrogel surfaces, resulting in highly controlled platelet adhesion. Further insights into the protein immobilization and platelet adhesion processes are provided by studies using imaging surface plasmon resonance. The hydrogel surfaces used in this work appear to provide an ideal platform for cell adhesion studies of platelets, and potentially also for other cell types. 相似文献
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声盘、视及光的亚微米圆弧线一般通过激光刻录机光刻制造，本文结合我国开发的用于磁盘伺服图形录写的激光刻录机，从机械结构、光路系统及计算机控制系统等方面，对其进行了研究分析。 相似文献
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随着激光扫描共聚焦显微镜技术的不断更新,功能不断拓展,已成为新兴学科纳米毒理学及其交叉学科科学研究的重要工具.激光扫描共聚焦显微镜能够动态检测纳米颗粒在活细胞内的定位及其与细胞骨架、细胞内离子、生物大分子的相互作用,有助于深入研究纳米颗粒对机体的毒理学效应及其机制. 相似文献
