Utilization of Pork Collagen Protein in Emulsified and Whole Muscle Meat Products

ABSTRACT: Effects of pork collagen in emulsified and whole muscle products were evaluated. Eight frankfurter treatments (0%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, and 3.5% pork collagen) and 4 ham treatments (0%, 1%, 2%, and 3% pork collagen) were formulated. Frankfurters and hams were evaluated for cooked yields, purge, color, texture, and sensory characteristics. Incorporation of pork collagen at 1% and above significantly ( P < 0.05) increased cooked and chilled yields in frankfurters but did not have any effect in hams. Purge was significantly ( P < 0.05) reduced in both frankfurters and hams after 4 wk of storage. Sensory difference testing showed no significant difference up to 2% usage level of pork collagen in both frankfurters and hams ( P > 0.05).     

Cottonseed Proteins in Frankfurters: Effect on pH, Cured Color, and Sensory and Physical Properties

Frankfurter-type sausages were made in which 5%, 10%, or 15% of the meat was replaced with the following cottonseed-derived protein products: genetically glandless cottonseed flour (CF); liquid cyclone processed deglanded cottonseed flour (LCPC); genetically glandless cottonseed storage protein isolate (CI). All-meat controls (0% cottonseed protein additive) were formulated at each replacement level with fat contents equivalent to those of the cottonseed protein-added frankfurters. Compared to all-meat controls, frankfurters made with increasing levels of cottonseed proteins generally had higher pH values, less cured color, less firmness of skin, softer texture, and were less desirable as judged by sensory panels. At the 10% and 15% replacement levels, frankfurters containing LCPC, had significantly (P < 0.05) lower scores for external and internal visual color, overall satisfaction and bioyield and rupture force values than did Cl-added frankfurters. Simple correlation coefficients suggest that significantly (P < 0.05) correlated responses exist within and across replacement levels between pH and sensory panel scores, Instron values and visual color scores.     

Sodium lactate and protective culture effects on quality characteristics and shelf-life of low-fat frankfurters produced with olive oil

Low-fat frankfurters (9% fat, 13% protein) were produced with olive oil and four levels of sodium lactate (0, 1, 2, 3%). The level of sodium lactate affected (p < 0.05) moisture and ash content, processing yield, skin strength and saltiness of low-fat frankfurters. Sodium lactate at 2% level appeared to be more beneficial for the production of low-fat frankfurters with olive oil. It had the highest score for flavour intensity and did not negatively affect skin strength, lextural and sensory properties or the colour of frankfurters. Low-fat frankfurters (9% fat, 13% protein) with olive oil, produced with 0% and 2% sodium lactate (SL) and protective culture (PC) and stored under vacuum at 4 °C, were compared with high-fat frankfurters (27% all animal fat, 11% protein). Low-fat frankfurters with olive oil had higher (p < 0.05) moisture and lower (p < 0.05) brine concentration than high-fat frankfurters. PC extended shelf-life of low-fat frankfurters with olive oil from 3 to 4 weeks. 2% SL kept the pH of frankfurters almost constant and extended shelf-life up to 6 weeks compared with 3 and 4 weeks shelf-life for low-fat and high-fat control frankfurters, respectively.     

COMPARATIVE STUDY OF CORN GERM AND SOY PROTEINS UTILIZATION IN COMMINUTED MEAT PRODUCTS1

Soy flour (SF), soy concentrate (SC) and corn germ protein flour (CGPF) at 3.5%, or 2% of soy isolate (SI) were incorporated in the formulations of frankfurters. There was no significant differences in proximate composition of frankfurters containing SF, SC. SI, and CGPF. Frankfurters formulated with high plant protein flour had lower cholesterol, and higher protein content than the all-meat control frankfurters. Control frankfurters had lower water holding capacity and higher cooking losses than those containing plant proteins. No significant differences (P < 0.05) were found in textural and color characteristics. Atypical aroma and flavor profiles increased in frankfurters with SF nd CGPF extension.     

Effect of glasswort (Salicornia herbacea L.) on the texture of frankfurters

This study was aimed at evaluation of the effect of glasswort levels (0, 0.5, 1, and 1.5%) on the textural properties of frankfurters formulated with 0.75% NaCl. The addition of glasswort improved protein solubility (P < 0.05) and apparent viscosity of frankfurters formulated with 0.75% NaCl, resulting in increased cooking yield and emulsion stability. This phenomenon might be mainly related to salts and dietary fiber within glasswort. In addition, the textural properties of frankfurter prepared with 0.75% NaCl and 1.5% glasswort showed similar properties (P > 0.05) compared to those of control (1.5% NaCl). Our result suggests that the use of glasswort can be an effective approach to manufacture reduced-salt meat products. Moreover, the addition of 1.5% glasswort in frankfurters formulated with 0.75% NaCl is the optimum level without adverse effect on the texture.     

Determination of sterigmatocystin in foods in Japan: method validation and occurrence data

ABSTRACT

A survey of the contamination of foods by sterigmatocystin (STC) was performed by an analytical method based on LC-MS/MS. STC was extracted from samples with acetonitrile/water (85/15, v/v) and then purified with immunoaffinity columns. The method was validated by a small-scale inter-laboratory study using spiked wheat samples. Mean recoveries of STC were 100.3% and 92.5% from two samples spiked at 0.5 and 5.0 µg/kg, respectively. A total of 583 samples were analysed between 2016 and 2018, and STC was detected in 19.9% of all samples at >0.05 μg/kg (limit of quantification). The foods that were contaminated by STC were wheat flour, Job’s tears products, rye flour, rice, buckwheat flour, white sorghum, barley products, azuki bean and corn flour. STC was not found in beer or wine. The occurrence of STC in domestic wheat flour (44.4%), Job’s tears products (41.7%) and rye flour (29.9%) accounted for the three highest values. The highest mean concentrations were obtained for Job’s tears products (0.3 μg/kg) and rye flour (0.3 μg/kg). The maximum contamination level was present in a sample of rye flour (7.1 μg/kg). Although the contamination levels were low, these results indicate that STC frequently contaminates Japanese retail foods. A continuous survey is required to assess exposure to STC in Japan.     

ADDITION OF DRY- AND WET-MILLED CORN GERM FLOURS TO MODEL SYSTEM FRANKFURTERS OF THREE FAT LEVELS1

Chemical, physical and sensory properties of cooked frankfurters of three fat levels with 0 and 3.5% dry-milled corn germ (DMG) flour and 0 and 3.5% wet-milled corn germ (WMG) flour added were investigated. As fat level increased, cooking losses increased and Instron compression values decreased, in cooked frankfurters with DMG and WMG flours. Addition of DMG flour decreased fat content and lightness (L) and increased yellowness (b-value) of cooked frankfurters. Sensory evaluation indicated that DMG flour in frankfurters decreased tenderness, juiciness and cured flavor intensity. The addition of DMG flour increased chewiness of frankfurters. WMG flour addition decreased fat and protein contents and lightness (L) and increased hue angle (due to higher b), Instron compression values and cooking loss. Cured flavor intensity decreased and off-flavor intensity increased with the addition of WMG flour to frankfurters.     

Functionality of Wheat Germ Protein in Comminuted Meat Products as Compared with Corn Germ and Soy Proteins

Wheat germ protein flour (WGPF), corn germ protein flour (CGPF), and soy flour (SF) were used as additives at a level of 3.5% in comminuted meat products (CMP). Frankfurters with protein additives showed increased water-holding capacity and batter stability and decreased cooking loss. Improved viscosity and adhesiveness were observed with protein additives when the level of added water was constant. Protein additives also influenced textural and sensory properties of frankfurters. WGPF at a level of 3.5% was found similar to effects of SF and CGPF. WGPF is a potential nonmeat protein additive that can be utilized as an extender in CMP such as frankfurters and bologna.     

以多糖为基质的脂肪模拟物替代动物脂肪在法兰克福香肠中的应用

将以多糖（魔芋粉、κ-卡拉胶和大麦β-葡聚糖）为基质的脂肪模拟物分别以20%、40%、60%和80%的比例替代猪脂肪用于法兰克福香肠生产中,探讨猪脂肪的不同替代比例对法兰克福香肠品质特性和感官特性的影响。结果表明,随着脂肪模拟物替代比例的增加,香肠的水分含量和碳水化合物含量显著增加（P<0.05）,而总脂质含量、蛋白含量、灰分含量、能量值和脂肪卡路里值显著降低（P<0.05）。而且,随着脂肪替代物替代量的增加,香肠的蒸煮损失率、乳化稳定性显著降低（P<0.05）,亮度值增加,硬度以及咀嚼性降低。另外,低场核磁结果表明替代脂肪能够显著缩短香肠的弛豫时间（P<0.05）,说明其能增强蛋白质网络对水分子的束缚能力。然而,较高的脂肪替代比例（60%和80%）显著降低肉糜在加热终点的储能模量（G′）和损失模量（G′′）（ P<0.05）,而且降低了法兰克福香肠的总体可接受性。上述研究结果表明,以多糖为基质的脂肪模拟物能够在法兰克福香肠中部分替代猪脂肪,且以40%的替代比例为最佳。     

EFFECT OF CARRAGEENAN ON PROCESSING AND QUALITY CHARACTERISTICS OF LOW-FAT FRANKFURTERS

Three types of carrageenan (K, I, K+I) × 4 levels (0, 0.25, 0.5 and 1.0%) were evaluated with a complete factorial design to characterize the effect of carrageenans on processing and quality characteristics of low-fat frankfurters (9% fat, 13% protein). l-Carrageenan at 0.5–1.0% level appeared to be more beneficial for the production of low-fat frankfurters. It reduced (P<0.05) the hardness of the skin and contributed to a softer (P<0.05) product and higher overall acceptability. Low-fat frankfurters (9% fat) manufactured with I-carrageenan (IC) or in combination with finely ground toasted bread (TB), isolated soy protein (ISP) and pork skin (PSK), were compared to high-fat (27% fat, 11% protein) and to low-fat (9% fat, 13% protein) control frankfurters prepared with 3.5% potato starch (PS), as well as with commercial frankfurters (25% fat, 11.6% protein, 4.5% starch). Frankfurters with IC had higher (P<0.05) moisture, softer (P<0.05) skin and the highest (P<0.05) separation of gelatin. However, textural properties and overall acceptability were similar (P>0.05) to commercial and high-fat control frankfurters. Addition of TB had no significant effect. Use of ISP and PSK improved (P<0.05) the water-binding capacity but negatively affected the overall acceptability of frankfurters.     

MEASURING CHARACTERISTICS OF FRANKFURTERS EXTENDED WITH SORGHUM FLOUR1

Inclusion of 2, 3.5 or 5% sorghum flour in frankfurters had no significant influence on pH, viscosity or thermal stability of batters. Water‐holding capacity was lower for batter containing 5% sorghum flour. The level of sorghum flour had no effect on proximate composition, mechanical, sensory or shelf‐life measurements. Leucine and isoleucine levels and saturation index decreased with the incorporation of 5% sorghum flour. Storage period at 6/pm 1C also had no effect on sensory measures of frankfurters. However, water activity and TBA values of frankfurters increased after 15 days of storage; no further increases were found after 30 days. Microbial colony forming units could be detected only after 30 days and did not increase further. Total volatile nitrogen did not increase until day 40 of storage. No interactions were found between level of sorghum flour and day of storage for any of the parameters tested. Up to 5% sorghum flour can be used in frankfurters without adverse effects.     

Heating rate effects on high-fat and low-fat frankfurters with a high content of added water

A study was made of the effect of heating rate (0.55, 1.10 and 1.90 °C min(-1)) on binding properties (cooking and purge losses) and texture (compression test) of high-fat (23%) and low-fat (9%) high added-water (19.8%) frankfurters during chilled storage (2 °C). Low-fat samples exhibited poorer (p < 0.05) binding properties; they were less hard and chewy (p < 0.05) but more cohesive and springy (p < 0.05) than high-fat frankfurters. Heating rate had little effect on binding properties. Hardness, cohesiveness, springiness and chewiness were greater (p < 0.05) at the slowest heating rate than at the other rates, behaviour being similar at the two higher rates.     

Potato Starch and Flour in Frankfurters: Effect on Chemical and Sensory Properties, and Total Plate Counts

Potato starch and flour were examined as possible constituents in frankfurter formulations. Frankfurters manufactured with 3% potato starch or 1.5% potato starch plus 1.5% potato flour in place of 3% wheat flour normally used as a binder, and stored at 4–5°C for 28 days did not differ significantly in percent shrink, water, protein, fat, ash or salt. Residual nitrite levels decreased during the storage period. Total plate counts reflect residual nitrite levels since the frankfurters (3% starch) with the lowest nitrite concentration also had the greatest number of bacteria (6.4 × 10⁶/g) on day 28. Frankfurters formulated with 1.5% potato starch plus 1.5% potato flour were rated more tender and more juicy than those made with 3% potato starch (P≤0.05 and P≤0.01) and 3% wheat flour (P≤0.01 and P≤0.01), respectively. Fresh potato starch and wheat flour franks did not differ from each other in either characteristic but after one week's storage the potato starch samples were rated more tender (P≤0.05) and more juicy (P≤0.01). Less force was required to shear the frankfurters formulated with 3% potato starch.     

Optimization of salt, olive oil and pectin level for low-fat frankfurters produced by replacing pork backfat with olive oil

Response surface methodology was used to determine the optimum salt level (1.3-2.1%) and pectin level (0.25-1.0%) when olive oil replaced pork backfat (0-100%) for the production of highly acceptable low-fat frankfurters (9% fat, 13% protein). The test ingredients significantly affected (P<0.05) jelly separation of the batter, skin strength, hardness, saltiness, odour and taste and the overall acceptability of the low-fat frankfurters. Batters with high pectin levels recorded the highest (P<0.05) jelly separation. Low-fat frankfurters with high salt levels tended to have very hard skins and increased (P<0.05) saltiness while those with a high pectin level were very soft, tasted like cream and had the lowest (P<0.05) score for odour and taste. The low-fat frankfurters with 1.8-2.1% salt, 0-35% olive oil and 0.25-0.45% pectin had the highest overall acceptability. However, low-fat frankfurters produced with 1.3% salt, 0.25-0.30% pectin and 80-100% olive oil were also acceptable. Such low-fat frankfurters, compared to commercial products, have a 48% lower salt content (from 2.5 to 1.3%) and 66.6% lower fat content (from 30 to 10%), in which 80-100% of the added fat is olive oil. Further research is needed to improve the acceptability of these frankfurters.     

Effect of dietary fish oil on fatty acid composition, lipid oxidation and sensory property of chicken frankfurters during storage

Broilers fed with three levels of fish oil (0, 2 and 4%) for 6 weeks were used in the manufacture of chicken frankfurters. The meat samples were vacuum-packed and stored at 0°C for 0, 10, 20 and 30 days. Cooking yield, proximate and fatty acid composition, lipid oxidation, microbial and sensory properties of the meat products were measured. Cooking yield, moisture, fat, protein, ash and cholesterol contents of frankfurters were not affected (P>0.05) by levels of dietary fish oil. Frankfurters processed from chickens fed diets enriched with 2 and 4% of supplemental fish oil had a higher (P<0.05) level of n-3 fatty acids (eicosapentaenoic acid and docosahexaenoic acid), but had a lower level of n-6 fatty acids (P<0.05) than the controls (0% fish oil). TBA values and pH values of meat samples were not affected (P>0.05) by levels of supplemental fish oil in the diets, however, pH values of the vacuum-packaged chicken frankfurters decreased (P<0.05) as the storage time increased. No differences (P>0.05) in total anaerobic plate counts were observed among the fish oil treatments during storage. Supplementation of 2 and 4% fish oil in the diets did not increase (P>0.05) the intensity of fishy flavor in chicken frankfurters.     

Total phenolic content, consumer acceptance, and instrumental analysis of bread made with grape seed flour

Grape seed flour (GSF) from grape pomace, a waste product generated during winemaking, was explored for use in bread production due to its potential health benefits. This study evaluated the consumer acceptance and physical properties of bread, including total phenolic content (TPC), made with varying levels of GSF. Dough and breads were prepared using different levels of replacement of hard red spring wheat flour (HRS) with GSF (0 to 10 g GSF/100 g HRS) and stored for 0, 2, or 6 wk at -20 °C. Replacement of 10 g GSF/100 g HRS increased the bread TPC from 0.064 mg tannic acid/g dry weight to 4.25 mg tannic acid/g dry weight. Consumer acceptance and instrumental analyses were used to investigate changes in sensory and texture properties due to GSF replacement. Replacement above 5 g GSF/100 g HRS decreased the loaf brightness and volume, with an increase in the bread hardness and porosity. Generally, breads containing ≥ 7.5 g GSF/100 g HRS were characterized by lower consumer acceptance. A reduction in overall and bitterness acceptance was observed in bread at 10 g GSF/100 g HRS, with decreased acceptance of astringency and sweetness at 7.5 and 10 g GSF/100 g HRS. Based on these results, the replacement of 5 g GSF/100 g HRS is recommended for the production of fortified breads with acceptable physical and sensory properties and high TPC activity compared to refined bread. PRACTICAL APPLICATIONS: This study shows that grape seed flour (GSF) can be used to replace hard red spring wheat flour (HRS) in bread production, with moderate impact on the physical and sensory properties of the bread. Replacement of up to 10 g GSF/100 g HRS significantly decreased overall consumer acceptance of the bread, with lower consumer acceptance of sweetness and astringency at 7.5 and 10 g GSF/100 g HRS. Thus, a replacement value of 5 g GSF/100 g HRS is recommended for the production of fortified breads.     

Influence of protein isolate from lupin seeds (Lupinus albus ssp. Graecus) on processing and quality characteristics of frankfurters

Lupin protein isolate (92% protein) from seeds of Lupinus albus ssp. Graecus (LSPI) was used as powder ingredient for the manufacture of frankfurters at levels 0, 1, 2 and 3% of the formulation weight. Additional 1% water was added during batter formulation to each 1% protein used. LSPI increased (P < 0.05) the pH and viscosity of batter and reduced the jelly separation. Increasing the LSPI level resulted in higher (P < 0.05) processing yield and lower (P < 0.05) purge accumulation, redness and visual colour scores and hardness of frankfurters. Significant differences in overall acceptability were not found among the control and frankfurters with 1% and 2% LSPI. Frankfurters with 3% LSPI were judged as unacceptable. Incorporation of LSPI at 1% level either in hydrated form or as stabilizer in pre-emulsified fat improved the processing characteristics and overall acceptability of frankfurters made with LSPI as powder ingredient and did not affect the color and texture.     

The effects of carrageenan and pectin on some quality characteristics of low-fat beef frankfurters

Effects of carrageenan (0.3, 0.5, or 0.7%) and carrageenan (0.3, 0.5, or 0.7%) with a pectin gel (20%) on some quality characteristics of low-fat beef frankfurters were evaluated in comparison to a high-fat control (HFC) and a low-fat control (LFC). While low-fat frankfurters had <3.0% fat, 73-76% moisture, 13-14% protein, HFC had 17% fat, 59% moisture, and 14% protein. A reduction of 50-59% in cholesterol was determined in low fat beef frankfurters as compared to HFC (P<0.05). Better process yield and emulsion stability, and less purge were observed with increasing carrageenan concentration. Treatment groups showed higher water holding capacity (WHC) than LFC, and lower WHC than HFC (P<0.05). With increasing carrageenan concentration, WHC increased and penetrometer value decreased in low-fat frankfurters.     

The effect of whey protein-enriched fractions on the physical and sensory properties of frankfurters

Four Beta-lactoglobulin (β-lg) enriched fractions containing different mineral contents were prepared and evaluated in frankfurters. Frankfurters were assessed for cook loss, water holding capacity (WHC), textural and sensory characteristics. The addition of the β-lg fractions reduced the cook loss (p<0.001) in comparison to the control (6.63% vs 3.98%). The fractions (β-lg 1 and 2) with the lowest calcium level significantly reduced WHC (p<0.01). The β-lg fractions had no detrimental effect on the sensory characteristics (p>0.05). All of the fractions increased the TPA value of hardness in comparison to the control (p<0.001) while the springiness decreased in the fractions (p<0.001) with the lowest mineral level. This study showed that the mineral composition of the β-lactoglobulin fractions affected cook loss, tenderness and hardness (TPA) of the frankfurters and the addition of the β-lactoglobulin enriched fraction did not affect the organoleptic quality of frankfurters in comparison to the control. This study shows the potential for next generation whey protein fractions and their application in meat products.     

Changes on Dough Rheological Characteristics and Bread Quality as a Result of the Addition of Germinated and Non-Germinated Soybean Flour

The increasing interest in functional and healthy food products has promoted the use of germinated soybean flour in the manufacture of foods for human consumption. Considering the beneficial effects of soy and its germination, farinograph and extensograph were used to study the effect of adding defatted flour of germinated (32 °C, 72 h) or non-germinated soybean—at different dry protein ratios (0, 0.5, 1.0, 1.5%)—to wheat flour on: water absorption (WA), maximum consistency time (MCT), dough stability (S), maximum resistance to extension (R _max), and dough extensibility (L). Baking tests (straight-dough procedure) were also performed to evaluate the effect of this addition on bread characteristics: loaf volume, texture (firmness, compression force, resilience), color (L*, a*, b*), crumb–grain structure (cell density, mean cell area, shape factor), and consumer acceptance (sensory analysis). Addition of both kinds of soybean flours increased the values of farinographic parameters (WA, MCT, S), although they did not have significant effects (p > 0.05) on extensographic properties (R _max, L). Loaf volume and crumb color were improved as soy flour addition was increased, whereas crust color was not affected (p > 0.05). Texture analysis showed that the addition of soy flour produced breads similar or better than the control, whereas the addition of GSF produced a coarser crumb grain. No detectable differences were found among samples during the sensorial analysis. Germinated soybean flour was better to improve dough breadmaking properties.