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Meyer C Meyer D Bickle TA Giese B 《Chembiochem : a European journal of chemical biology》2003,4(7):610-614
Cleavage of DNA single and double strands at an 8-oxoguanine-containing nucleotide occurs in 90 % yield if the modified oligonucleotide is treated with NH(3) and O(2) at 60 degrees C. The mechanism of this oxidative cleavage reaction was studied, and the reaction was applied to the generation of single-stranded overhangs on PCR-amplified DNA that can be ligated. As an example, the lac Z' gene was amplified by PCR with 8-oxoguanine modified primers, restricted by ammonia treatment, ligated into a plasmid vector, transformed in Escherischia coli cells, and screened for blue colonies. This method guarantees efficiencies comparable to the standard cloning procedure with restriction enzymes, and it allows the design of any 3'-overhang independent of the sequence of the cloned DNA. 相似文献
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Polymerase Synthesis and Restriction Enzyme Cleavage of DNA Containing 7‐Substituted 7‐Deazaguanine Nucleobases
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Michaela Mačková Soňa Boháčová Dr. Pavla Perlíková Dr. Lenka Poštová Slavětínská Prof. Dr. Michal Hocek 《Chembiochem : a European journal of chemical biology》2015,16(15):2225-2236
Previous studies of polymerase synthesis of base‐modified DNAs and their cleavage by restriction enzymes have mostly related only to 5‐substituted pyrimidine and 7‐substituted 7‐deazaadenine nucleotides. Here we report the synthesis of a series of 7‐substituted 7‐deazaguanine 2′‐deoxyribonucleoside 5′‐O‐triphosphates (dGRTPs), their use as substrates for polymerase synthesis of modified DNA and the influence of the modification on their cleavage by type II restriction endonucleases (REs). The dGRTPs were generally good substrates for polymerases but the PCR products could not be visualised on agarose gels by intercalator staining, due to fluorescence quenching. The presence of 7‐substituted 7‐deazaguanine residues in recognition sequences of REs in most cases completely blocked the cleavage. 相似文献
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Cover Picture: Fluorogenic Sequencing Using Halogen‐Fluorescein‐Labeled Nucleotides (ChemBioChem 8/2015)
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Zitian Chen Prof. Haifeng Duan Shuo Qiao Wenxiong Zhou Haiwei Qiu Li Kang Prof. X. Sunney Xie Prof. Yanyi Huang 《Chembiochem : a European journal of chemical biology》2015,16(8):1125-1125
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Cover Picture: One‐Pot Chemoenzymatic Cascade for Labeling of the Epigenetic Marker 5‐Hydroxymethylcytosine (ChemBioChem 13/2015)
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Gil Nifker Dr. Michal Levy‐Sakin Dr. Yifat Berkov‐Zrihen Dr. Tamar Shahal Tslil Gabrieli Dr. Micha Fridman Dr. Yuval Ebenstein 《Chembiochem : a European journal of chemical biology》2015,16(13):1825-1825
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Inside Cover: Traceless Cleavage of Protein–Biotin Conjugates under Biologically Compatible Conditions (ChemBioChem 17/2017)
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Dr. Joseph Cowell Dr. Matthew Buck Dr. Ali H. Essa Rebecca Clarke Prof. Waldemar Vollmer Dr. Daniela Vollmer Dr. Catharien M. Hilkens Prof. John D. Isaacs Dr. Michael J. Hall Dr. Joe Gray 《Chembiochem : a European journal of chemical biology》2017,18(17):1670-1670
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《Chembiochem : a European journal of chemical biology》2003,4(6):457-457
The cover picture shows a ligand‐targeted proteinase enzyme or “catalytic antagonist” acting as a molecular angler fish: By precisely positioning different binding ligands (L) around the active site “mouth” of a degradative proteinase enzyme, target proteins (TP) can be plucked from solution, locked in position adjacent to the catalytic triad “jaws”, and in this way readily and specifically degraded. The hunting strategy of the deep sea angler fish, which uses a lure above its mouth, illustrates this principle. Further details can be found in the article by B. Davis, R. R. Bott, J. B. Jones et. al. on pp. 533–537. 相似文献
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Cover Picture: DNA Antenna Tile‐Associated Deoxyribozyme Sensor with Improved Sensitivity (ChemBioChem 21/2016)
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Amanda J. Cox Hillary N. Bengtson Dr. Yulia V. Gerasimova Dr. Kyle H. Rohde Dr. Dmitry M. Kolpashchikov 《Chembiochem : a European journal of chemical biology》2016,17(21):1995-1995
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Prof. Dr. Emily R. Derbyshire Dr. Vanessa Zuzarte‐Luís Dr. Andreia D. Magalhães Dr. Nobutaka Kato Dr. Paul C. Sanschagrin Dr. Jinhua Wang Dr. Wenjun Zhou Dr. Chandrasekhar V. Miduturu Prof. Dr. Ralph Mazitschek Prof. Dr. Piotr Sliz Prof. Dr. Maria M. Mota Prof. Dr. Nathanael S. Gray Prof. Dr. Jon Clardy 《Chembiochem : a European journal of chemical biology》2014,15(13):1841-1841