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白腐菌Trametes versicolor产漆酶发酵条件的优化 总被引:8,自引:0,他引:8
对白腐菌Trametesversicolor产漆酶的发酵条件进行了研究,结果表明摇瓶培养产漆酶的最佳培养基组成为:可溶性淀粉2g/L,氯化铵1.2g/L,KH2PO42g/L,MgSO4·7H2O0.5g/L,CaCl2·2H2O0.1g/L,VB10.001g/L,Tween802g/L,微量元素混合液7mL/L,愈创木酚0.015mmol/L,CuSO4·5H2O60μmol/L,pH3.5;最佳发酵条件为:在250mL三角瓶装50mL培养基,接种量(Φ8mm)2块,25℃,150r/min振荡培养10d时,漆酶活力达到862U/L,约是优化前的4倍。 相似文献
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研究了碳源、氮源及诱导剂添加方式对白腐菌P.conchatus液体培养产漆酶的影响.结果表明,当碳源为葡萄糖,氮源为酵母膏,C/N比为15∶1时,P.conchatus合成漆酶酶活达到8795U·L-1;在初始培养基中,添加1.5%麦麸与1 mmol/L Cu2+共同诱导漆酶合成,酶活可达到51004U·L-1;将Cu2的添加时间改在发酵后第4d,添加量为3mmol/L,其他条件不变,漆酶酶活达到101968U·L-1,比在初始培养基中添加1 mmol/L Cu2+所达到的酶活高了近两倍. 相似文献
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白腐菌处理漂白废水的研究 总被引:3,自引:0,他引:3
经驯化后的白腐菌处理苇浆CEH漂白废水比未驯化菌的处理效果好,在最适培养条件(加0.1%葡萄糖,0.02%酒石酸铵,0.05%HAc-NaAc,pH值4.3-4.5,32℃-42℃,培养五天)下间歇式连续处理,处理效果较稳定,CODcr去除率49.1-55.9%,TOCI去除率43.7%-49.0%,脱色率为63.1-67.9%。 相似文献
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白腐菌预处理对杨木化学制浆性能的影响 总被引:18,自引:4,他引:18
研究了杨木经白腐菌Phellinus Pini预处理前后化学组成的变化;木片腐朽前后扫描电镜分析;预处理对后续KP蒸煮及浆料性能的影响.结果表明,采用该白腐菌预处理半个月的结果优于1个月的.前者蒸煮浆料在与对照浆相近的卡伯值情况下能节省1个百分点的用碱量或缩短30min保温时间,而得率分别提高3.19和4.13个百分点,撕裂指数分别提高17.9%和27.1%;裂断长分别提高19.6%和24.6%;耐破指数分别提高23.5%和32.2%. 相似文献
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Gabriella Di Lena Elvira Patroni G. B. Quaglia 《International Journal of Food Science & Technology》1997,32(6):513-519
Summary This investigation was concerned with the solid-state cultivation of the white-rot fungus Lentinula edodes on wheat bran and its impact on the nutritional value of the substrate. A considerable reduction (60%) in the concentration of the phytic acid of wheat bran was detected after 7 days of incubation, but no further reduction was observed during the following weeks. Protein digestibility increased gradually during the first 3 weeks of incubation reaching a maximum (82%) at day 20. Hydrolytic and oxidative exoenzymes, such as phytase, endocellulase and polyphenol oxidase, were released by the mycelium during the colonization of the substrate. Total and insoluble dietary fibre and crude protein content in bran increased with fungal growth while the in vitro dry matter enzyme digestibility decreased. 相似文献
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以小麦麸皮为原材料,通过布拉迪酵母发酵处理小麦麸皮降低植酸含量,提高可溶性戊聚糖(WEAX)和总酚含量以改善小麦麸皮的营养价值。研究固态发酵过程中小麦麸皮的基本组分与活性成分的变化,结果表明,经过发酵处理小麦麸皮的总膳食纤维(TDF)与不可溶性膳食纤维(IDF)含量显著提高,可溶性膳食纤维(SDF)含量提高但不显著;WEAX和总酚含量均呈现先增加后降低的趋势,最大分别增加了12.95倍、1.45倍;植酸含量显著降低,最大降解率为74.67%。 相似文献
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Feng Kong Lan Wang Hongzhang Chen Xuan Zhao 《International Journal of Food Science & Technology》2021,56(1):287-292
Steam explosion (SE) was exploited for stabilizing wheat bran (WB), and its effects on nutrients retention of WB were investigated. Result showed that SE could effectively inactivate lipase and peroxidase of WB at 0.8 MPa (170 °C) for 5 min while lipid and protein were not loss. Total flavonoids, phenolics contents, soluble dietary fibre content and DPPH radical scavenging activity in WB after SE increased by 198%, 83%, 27% and 21% respectively compared with those after conventional thermal sterilization (0.1 MPa (121 °C) for 20 min), while fatty acid value and peroxide value and insoluble dietary fibre content deceased by 21%, 75% and 24%, respectively. SE inhibited hydrolysis rancidity of wheat bran and reconstituted whole wheat flour (wholemeal) in the accelerated storage test. Therefore, SE may be a new efficient technology that could stabilize cereal bran and further promote its products development. 相似文献
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对米糠进行固态发酵,研究其蛋白、脂肪、灰分和总糖含量的变化规律,以期找到一种有效深度开发米糠资源的方法。结果表明:蛋白和灰分含量相比于发酵前明显增加,脂肪和总糖含量随着发酵时间的延长逐渐降低。以真蛋白含量为响应值,对固态发酵条件进行响应面辅助优化,确定最佳发酵条件为温度37℃,时间102h,水分含量60%,初始pH 5.5,接种量20 mL/100 g,发酵米糠的真蛋白含量19.65%,比原米糠提高了41.88%。此外,对米糠的氨基酸组成进行分析,蛋氨酸和苏氨酸占总氨基酸含量分别提高了73.92%和22.99%。 相似文献
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Sen Ma Zhen Wang Ning Liu Peng Zhou Qingdan Bao Xiaoxi Wang 《International Journal of Food Science & Technology》2021,56(4):1623-1630
The effects of wheat bran dietary fibre (WBDF) on the rheological properties of dough during fermentation and quality of Chinese steamed bun (CSB) were investigated. The study revealed that with the increase of the content of WBDF, the dough extensibility and time at which gas starts to escape from the dough significantly decreased but the dough firmness significantly increased (P < 0.05). The elastic modulus and viscous modulus showed an upward trend, probably due to the increased molecular weight of the viscoelastic body resulting from the presence of WBDF. Additionally, by changing the quality of the gluten network, the specific volume and L* value significantly decreased from 2.52 to 1.31 mL g−1, and from 87 to 51, respectively, these adverse effects on CSBs could be moderated by the fermentation process. 相似文献
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目的 研究麦麸对酥性饼干颜色、物理性质以及淀粉消化性的影响。方法 将麦麸按0~50%比例添加到面粉中制作饼干,测定混合粉的溶剂保持力,测定饼干颜色、延展度、硬度以及淀粉消化性。结果 添加麦麸导致混合粉的水和碳酸钠溶剂保持力值增加,乳酸溶剂保持力值和面筋性能指数降低(4.71%~33.20%和25.73%~34.11%),饼干颜色变暗。当麦麸添加量大于20%时,饼干的延展度和硬度增大(53.54%~64.31%和135.98%~408.53%)。此外,麦麸的添加在一定程度上降低了饼干淀粉消化性。麦麸添加量与饼干物理性质呈强正相关性,与消化终点葡萄糖含量呈负相关。通过主成分分析,提取了2个主成分,累计方差贡献率为85.3%。结论 适量添加麦麸有效改善了饼干酥脆性,并在一定程度上抑制了饼干淀粉的消化,增加了饼干的功能性质,可以为麦麸产品的进一步开发提供参考。 相似文献