影响消费者购买云南白药牙膏的因素分析

通过实地调研,应用Logistic回归模型,研究了影响有购买牙膏需求的消费者购买云南白药牙膏的因素,提出了如何估计有购买牙膏需求的消费者购买云南白药牙膏的概率.得到的结论是牙龈出血和牙龈肿痛是人们购买云南白药牙膏的两个最重要的原因,口腔异味使购买白药牙膏的人数显著减少,可能原因是云南白药牙膏价格太贵.建议云南白药牙膏市场定位应是需要治疗或者预防引起牙龈出血、牙龈肿痛的各种口腔疾病的患者.     

含锌生物活性微晶玻璃的制备及研究

本工作通过引入ZnO制得了一种对人体代谢机能有促进作用的ZnO-MgO-CaO-B_2O_3-SiO_2-P_2O_5系统新型的生物活性微晶玻璃人工骨材料,并对玻璃的组成范围与熔制条件,晶化特性与热处理工艺制度、晶相以及ZnO含量对材料性能的影响、材料的生物相容性和生物活性等方面进行了较系统的研究。结果表明:该材料以氧磷灰石和β-硅灰石为主晶相;具有较高的力学强度(抗折强度170Mpa、抗压强度500Mpa)和良好的化学稳定性,在模拟生理体液中,可有控制地释出促进人体伤口愈合的Zn~(2+)离子。动物实验证明该材料具有良好的生物相容性和生物活性,有着良好的应用前景。     

Anti-biofilm effect of glass ionomer cements incorporated with chlorhexidine and bioactive glass

The effect of glass ionomer cement and resin-modified glass ionomer cement incorporated with chlorhexidine and bioactive glass on antimicrobial activity and physicochemical properties were investigated.The experimental results showed that groups incorporated with 1% chlorhexidine exhibited a significant reduction of optical density values of the bacterial suspension and increased the degradation of Streptococcus mutans biofilm.However,groups incorporated with 10% bioactive glass did not affect the optical density values and the biofilm formation.The mechanical properties of the materials and the polymerization were not influenced by the addition of chlorhexidine.Nevertheless,the compressive strength was lower when the materials were incorporated with bioactive glass.It can be concluded that glass ionomer cements incorporated with chlorhexidine can maintain its mechanical properties as well as reduce early S mutans biofilm formation.Controlled release/sustained release technology may be required to optimize the antibacterial activity of glass ionomer cements incorporated with bioactive glass.     

大鼠5周高住低练减体重效果研究

将24只雄性SD大鼠随机分为常氧安静组（Ⅰ组）、低住低练组（Ⅱ组）、间歇低氧安静组（Ⅲ组）、高住低练组（Ⅳ组）,进行5周训练.采用称量法测定大鼠体重、食物摄入量,大鼠肾周、附睾脂肪垫及腓肠肌重量.5周末Ⅳ组大鼠体重低于Ⅰ、Ⅲ组（P〈0.05）和Ⅱ组（P〉0.05）,体重增幅低于其他各组（P〈0.05）.Ⅳ组大鼠脂肪垫总量低于Ⅲ组（P〈0.05）和Ⅰ组（P〉0.05）,腓肠肌重量高于Ⅲ组（P〈0.05）和Ⅰ组（P〉0.05）.试验中Ⅳ组大鼠食物摄入总量较其他各组大鼠低.5周高住低练具有良好的减体重效果,高住低练过程中食物摄入量减少是大鼠体重下降的一个重要因素.     

STUDY OF ARTIFICIAL EYES HAVING HIGH STRENGTH AND LOW DENSITY

The artificial eyes having high strength , law density and natural movement, which are made from hydroxyapatite and bioactive glass have been utilized effectively in clinic. The technology of compounding hydroxyapatite, melting bioactive glass and making the artificial eyes are stated in this paper. In animal test, the bioactivity and biocompatibility of the material making the artificial eyes are examined by histological observation, SEM XRD and electronic probe. At the same time, application of the artificial eyes in clinic are also introduced.     

富金花茯茶水提物对四氧嘧啶诱导糖尿病小鼠的降血糖功效研究

研究富金花茯茶水提物对四氧嘧啶诱导糖尿病小鼠的降血糖功效.采用四氧嘧啶诱发糖尿病小鼠,随机分为模型组、阳性药组、富金花茯茶低剂量组、富金花茯茶高剂量组、黑毛茶低剂量组、黑毛茶高剂量组6个组,另选10只健康小鼠作为空白组.每4 d记录小鼠体重及进食量,每6 d断尾检测空腹血糖值.末次灌胃后禁食16 h,断尾检测空腹血糖值;并摘眼球取血,离心取上清按照试剂盒方法测定血清中TC、TG、HDL、LDL含量.结果表明,富金花茯茶、黑毛茶水提取均能缓解糖尿病小鼠消瘦和多食多尿的症状.富金花茯茶与黑毛茶均能有效降低糖尿病小鼠血糖值（P〈0.05）,其中富金花茯茶高剂量组小鼠血糖值明显低于黑毛茶高剂量组（P〈0.05）;且两组提取物均能降低小鼠血清中TC、TG、HDL浓度,对LDL和肝体比基本无影响.综上所述,富金花茯茶的降糖功效要明显优于原料黑毛茶.     

“循环通络”乳膏剂制备及其抗类风湿性关节炎的考察

以综合评分为指标,通过正交设计试验优选“循环通络”乳膏剂处方,并采用单因素试验对其制备工艺参数进行了优化.研究表明,乳膏剂的最佳处方为浓缩药液200 g、凡士林90 g、羊毛脂10 g、液体石蜡80 g、单硬脂酸甘油酯50 g、硬脂酸65 g、丙三醇150 g、吐温80 40 g、月桂氮艹/卓酮10 g、尼泊金乙酯0.5 g,用水加至1 000 g.制备的最佳工艺为油、水相分别加入乳化,搅拌速度为250 r·min^-1,乳化温度为70 ℃,乳化时间为15 min.抗类风湿性关节炎实验     

离断不同空肠距离对2型糖尿病小型猪降糖分析

为明确Trietz韧带下离断不同空肠距离对2型糖尿病（Type 2 diabetes,T2DM）滇南小耳猪动物模型术后血糖的影响,将20只雌性滇南小耳猪随机分为正常对照组（NC组）、糖尿病对照组（DC组）、糖尿病手术1组（DO1组）和2组（DO2组）,每组5只.DC、DO1及DO2组采用高糖高脂饮食加小剂量链尿佐菌素诱导建立T2DM动物模型.建模成功后（第8周末）DO1组胃转流术（Roux-en-Y gastric bypass,RYGB）（Treiz韧带下3m离断空肠）,DO2组RYGB（Treiz韧带下6m离断空肠）,监测各组术前、术后2、4及8周空腹血糖、血清胰岛素、胰高血糖素样肽-1（Glp-1）、葡萄糖依赖性促胰岛素释放肽（GIP）,并比较抵抗指数（HOMA-IR）变化.与DC组相比,DO1、DO2组术后血清胰岛素和Glp-1浓度明显升高（P＜0.05）,胰岛素抵抗明显改善（P＜0.05）;DO1、DO2组空腹血糖和GIP浓度明显下降（P＜0.05）,DO2组血糖下降更明显（P＜0.05）.实验显示：Treiz韧带下更远距离离断空肠可获更好降糖效果.     

云南某铅锌矿全尾砂膏体室内实验研究

对云南某铅锌矿采用全尾砂膏体充填方案开展了室内实验研究,分析了全尾砂基本物理化学性质（粒级组成、化学成分）,并进行了全尾砂自然沉降试验、坍落度试验、全尾砂试块单轴强度试验,获得了全尾砂及全尾砂胶结料的自然沉降特性、流动性能、析水性能及强度特性.试验结果显示：全尾砂自然沉降2~3 h后,重量浓度达到72%~76%,需要添加絮凝剂加快沉降;全尾砂胶结料的重量浓度为78%~79%时,坍落度为24~17 cm,基本无析水（1%~0.6%）,充填料呈膏体状态,适合长距离输送;全尾砂膏体配合比在1∶4~1∶12时,28 d单轴抗压强度达到1~4 MPa.室内实验初步表明该矿山可以采用全尾砂膏体充填方案.     

花椒麻素对SD大鼠肥胖模型脂代谢的影响

旨在研究花椒麻素对肥胖症模型SD大鼠体内脂质代谢功能的影响.将48只SD雄性大鼠按体质量分为空白对照组、高脂模型组、花椒麻素低/中/高剂量组[4、8、12?mg/(kg·d)]、非诺贝特阳性对照组[40?mg/(kg·d)],经过灌胃6周,记录大鼠的初质量、末质量及采食量,计算实验动物饲料效率.解剖后分别测定SD大鼠血...     

纳米介孔生物活性玻璃的制备及研究

本文用模板法制备了一种纳米介孔生物活性玻璃,通过动态光散射和电镜对其进行了表征。同时研究了这种纳米介孔生物活性玻璃在模拟体液中的生物活性,实验结果用小角X射线衍射,傅里叶变换红外光谱和扫描电镜表征,确定这种纳米介孔生物活性玻璃在模拟体液48小时以后就能形成羟基磷灰石结晶的沉积,同时电镜观察纳米颗粒出现了团聚。用3-氨丙基-三乙氧基硅烷（APTES）将这种生物玻璃表面氨基化后,将胶原固定其上,通过细胞培养确定胶原的固定能够有效的提高细胞在这种生物活性玻璃表面的生长情况。     

生物玻璃强度性质的改进方法

生物玻璃作为生物材料具有金属材料和有机材料无法比拟的优点，但是同时又存在诸如脆性大、抗弯强度不足等严重缺陷。本文就生物玻璃存在的不足主要从调节玻璃组分、改善玻璃制备工艺以及采用多种材料复合等方面阐述了提高生物玻璃某些性能的途径。     

Preparation and bioactivity of sol-gel macroporous bioactive glass

Bioactive glass is well known for its ability of bone regeneration,and sol-gel hioactive glass has many advantages com- pared with melt-derived bioactive glass.3-D scaffold prepared by the sol-gel method is a promising substrate material for bone tissue engineering and large-scale bone repair.Porous sol-gel glass in the CaO-SiO_2-P_2O_5 system with macropores larger than 100μm was prepared by the addition of stearic acid as a pore former.The diameter of the pore created by the pore former varied from 100 to 300μm.The formation of a hydroxyapatite layer on the glass was analyzed by studying the surface of the porous glass by scanning elec- tron microscopy,energy dispersive spectroscopy,X-ray diffraction,and Raman spectra after they had been immersed in simulated body fluid(SBF)for some time,and the porous glass shows good bioactivity.     

Anti-Hyperglycemic Activity of Shan Tang Ping an Ethanol Extract from Yew Tree in Diabetic Rats

The extracts of yew tree have showed unique anti-tumor and anti-leakematic activities. Taxol extracted from it is effective against a variety of cancers, such as ovarian cancer and breast cancer. However the hypoglycemic efficacy of the extract has not been reported yet, so the effects of oral administration of yew tree extracts on streptozotocin-induced diabetic rats were examined. Administration of shah tang ping （STP）, a fraction from the column separation of extract （450 mg/kg body weight）, for 14 days reduced the serum glucose concentrations of fasted diabetic rats by 52.4%. In addition, it also decreased the concentrations of triglyceride （TG）, total cholesterol （TC）, and low density lipoprotein cholesterol （LDL-C）. An oral glucose tolerance test （OGTT） was performed, a significant improvement in glucose tolerance was observed in rats treated with STP. These results suggest that STP has hypoglycemic properties and hypolipidemic action on streptozotocin-induced diabetic rats.     

医用生物活性材料的研究

本文以CaO-Al_2O_3-P_2O_5系统为基础玻璃;加入一定量的羟基磷灰石、磷酸氢钙,混合、成型后,经热处理而制得医用生物活性复合材料。用差热分析、X射线衍射分析、扫描电镜和电子探针X射线衍射分析鉴定了材料中的晶相和显微结构,并对该材料的气孔率、化学性质和弹性模量等性能进行了测试和分析。动物试验结果表明,此种材料具有良好的生物相容性和生物活性,是一种具有实用价值的新型人工骨材料。     

玉米须对Wistar大鼠神经行为的毒性作用

研究了玉米须对Wistar大鼠认知、学习、记忆及神经兴奋度等神经行为的毒性作用。选用测试组合自主活动箱,Morris水迷宫和避暗程序自动控制仪对连续77天经口接受不同剂量玉米须的Wistar大鼠进行测试。自主活动试验结果表明:雄性动物5min平均自主活动量明显高于雌性动物,但各性别动物之间无显著性差异。水迷宫试验显示:雌性试验各组平均游泳距离明显低于雌性对照组,雄性高剂量组动物平均游泳速度提高显著性高于雄性其余各组,其他试验参数之间无显著性差异。避暗试验显示:玉米须各剂量组对Wistar大鼠记忆无毒副作用,相反具有一定促进作用,但无剂量效应关系。连续77天饲喂Wistar大鼠玉米须最高剂量达8.0%(相当于雄性大鼠玉米须日摄入量9.354g/kg体重,雌性大鼠为10.308g/kg体重),对大鼠神经行为无毒副作用,相反在一定程度上能促进动物的记忆能力。     

四氧嘧啶致小鼠、大鼠糖尿病模型研究

目的：研究四氧嘧啶尾静脉注射小鼠和大鼠诱导糖尿病模型,观察不同剂量四氧嘧啶诱导糖尿病模型的稳定性。方法l分别对小鼠尾静脉注射四氧嘧啶60、70、80mg/kg和对大鼠尾静脉注射四氧嘧啶30、40、50mg/kg,测定不同时点小鼠和大鼠的血糖值及体重。结果60、70和80mg/kg四氧嘧啶剂量均可导致小鼠糖尿病模型,但70和80mg/kg剂量组小鼠死亡率较大,而60mg/kg组小鼠糖尿病模型稳定;30mg/kg四氧嘧啶剂量不能造成大鼠糖尿病模型,40和50mg/kg四氧嘧啶剂量均可导致大鼠糖尿病模型,但四氧嘧啶50mg/kg剂量组大鼠死亡率较大,只有40mg/kg组糖尿病大鼠模型稳定。结论：造成小鼠和大鼠糖尿病模型最佳剂量分别为60mg/kg和40mg/kg。     

镧对大鼠回避学习能力的影响

为了探讨氯化镧(LaCl3)染毒对子代大鼠回避学习记忆能力的影响。采用健康成年雌性Wistar大鼠24只,随机分为对照组(饮用蒸馏水);低剂量染镧组(饮用含0.25%LaCl3的蒸馏水溶液)、中剂量染镧组(饮用含0.5%LaCl3的蒸馏水溶液)和高剂量染镧组(饮用含1.0%LaCl3的蒸馏水溶液),每组6只动物。自雌鼠受孕日至仔鼠断乳后1个月以自由饮水的方式摄入镧。通过穿梭箱实验判断仔鼠的主被动逃避学习能力。结果显示,中剂量组仔鼠主动逃避潜伏期和电击次数显著高于低剂量组和对照组(P0.05),电击时间显著高于对照组(P0.05);高剂量组仔鼠主动逃避潜伏时间、电击次数和电击时间均显著高于中剂量组、低剂量组和对照组(P0.05)。LaCl3染毒造成大鼠主动逃避能力下降,LaCl3染毒组仔鼠表现出剂量依赖的回避学习能力损伤(P0.05)。     

Tissue response of an osteoinductive bioceramic in bone defect rabbit model

HA/TCP and HA rods (?5 mm×10 mm) were made for implantation in New Zealand white rabbit with different condition. Sixty three rabbit were divided into three groups: group 1 (n=18), group 2 (n=27) and group 3 (n=18). In group 1, 10 mm radius was defected, and one HA/TCP rod was implanted in the muscle a distant away from the bone defect area. In group 2, also, 10 mm radius was defected, one HA rod was implanted in the muscle a distant away from the bone defect area. In group 3, two HA/TCP rods were implanted in the dorsal muscle of the rabbit with bone intact. Histological observation showed that in group 1, some new bone was found only two months after implantation (n=2), and obvious immature woven bone could be observed in these bioceramics from the 3^rd month on. However, in group 3, bone began to be found 6 months after implantation (n=2). In group 2, we could not find any bone tissue up to 9 month’s observation. These results suggest that, first, the bone defect model could significantly accelerate bone formation at non-osseous sites in rabbits; second,. HA/TCP bioceramics were confirmed with osteoinductive property while HA bioceramics without osteoinductive property nearly. Thus, bone defect might be a good animal model for further researches for osteoinductive bioceramics.