Amperometric Cholesterol Biosensor Using Layer-by-Layer Adsorption Technique on Polyaniline-coated Polyester Films

An amperometric cholesterol biosensor was fabricated using polyaniline-coated polyester films. Polyaniline was dissolved in chloroform with camphorsulfonic acid, and polystyrene was added to this solution. Using this mixed solution, the coating was placed onto polyester films. Cholesterol oxidase was immobilized onto these films using an electrostatic layer-by-layer adsorption technique. Poly(diallyldimethylammonium chloride) was used as the counter ion source. The level of adsorption was examined and evidence of layer-by-layer adsorption was investigated using a quartz crystal microbalance (QCM). A cholesterol biosensor was fabricated from these films as a working electrode, and it was used to measure the cholesterol concentration.     

新型聚苯胺纳米复合材料研究进展

系统综述了聚苯胺纳米复合材料的制备方法,结合典型实例详细论述了近年来研制的新型聚苯胺纳米复合材料的制备及性能,指出了聚苯胺纳米复合材料的研究方向。     

Cholesterol biosensor based on electrochemically prepared polyaniline conducting polymer film in presence of a nonionic surfactant

Cholesterol biosensor has been fabricated by covalently coupling cholesterol oxidase (ChOx) via glutaraldehyde onto electrochemically prepared polyaniline film in presence of TritonX-100 [4-(1,1,3,3-tetramethylbutyl) phenyl polyethylene glycol], a non-ionic surfactant onto indium-tin-oxide (ITO) glass substrate. These ChOx/PANI-TX-100/ITO bioelectrodes have been characterized using Fourier transform infrared (FTIR) spectroscopy, cyclic voltammetry (CV) and scanning electron microscopy (SEM) techniques. The results of response measurements carried out on ChOx/PANI-TX-100/ITO bioelectrodes using amperometric and photometric techniques, reveal detection limit as 5 mg/dl, linearity from 5 to 400 mg/dl of cholesterol and sensitivity as 131 μA/(mg/dl cm⁻²). These biosensing electrodes are thermally stable up to 65 °C, can be used about 20 times and have a shelf-life of about 10 weeks when stored at 4 °C. Attempts have also been made to utilize the ChOx/PANI-TX-100/ITO bioelectrodes for estimation of free cholesterol concentration in serum samples.     

多功能性聚苯胺/聚合物纳米复合材料的制备及应用

基于国内外最新研究文献及本课题组的研究,综述了多功能性聚苯胺/聚合物纳米复合材料的制备方法、性能及应用前景。聚苯胺/聚合物纳米复合材料可以由机械共混法、涂布法和原位聚合法,如分散聚合法、模板诱导聚合法及电化学聚合法制备得到。聚苯胺/聚合物纳米复合材料在透明导电塑料薄膜、防静电涂料、导电纤维、电致发光器件、电磁屏蔽材料等领域有着广阔的应用前景。     

导电高分子纳米复合材料研究进展

介绍了导电高分子纳米复合材料的特点,综述了导电高分子纳米复合材料的最新研究进展,展望了导电高分子纳米复合材料的发展前景。     

橡胶纳米复合材料制备研究进展

目前，橡胶纳米复合材料的制备方法主要有插层复合法，溶胶－凝胶法，原位分散法和纳米微粒直接共混法4种，综述了各种方法的制备机理，并总结了各种方法的优缺点。     

聚合物基纳米复合材料的结构与性能

叙述了由插层复合法、溶胶-凝胶法和纳米微粒直接共混法制备的聚合物基纳米复合材料的结构和性能。     

导电聚苯胺/二次插层有机土纳米复合材料的制备与性能研究

以氨基磺酸(SA)为掺杂酸,用二次插层的CTAB_2-Ni_1-MMT为无机主体,制备了PANI/CTAB_2-Ni_1-MMT纳米复合材料。研究了CTAB_2-Ni_1-MMT量对产物PANI/CTAB_2-Ni_1-MMT电导率的影响,并与以一次插层的CTAB-MMT为无机主体制备的PANI/CTAB-MMT进行了比较,结果表明PANI/CTAB_2-Ni_1-MMT的电导率较好,并对其进行了FT-IR、XRD、TG分析。     

Enzymatic Electrode Obtained by Immobilizing of Urease into a Nanocomposite Film Based on Conducting Polymers and Different Additives

The nanocomposite film was obtained by electrochemical co-polymerization of the corresponding monomers in the presence of functionalized carbon nanotubes and different additives. By immobilizing urease on a modified electrode of type nanocomposite film/platinum substrate a novel amperometric biosensor for the detection of urea was prepared. The polymeric and nanocomposite films were utilized as conducting films for amperometric urea sensing after covalent immobilization of urease onto film-coated electrode. The electrochemical performance of the modified electrode was studied by cyclic voltammetry, chronoamperometry, and scanning electron microscopy. The immobilized urease on the nanocomposite film surface exhibited an excellent electrocatalytic response toward reduction urea. Amperometric response was measured as a function of urea concentration at a fixed potential, in this case the open circuit potential. The obtained sensor showed rapid response and good reversibility. The response time was less than 10 s, while the reversible time was about 5–15 s. The enzymatic nanostructured electrode exhibited high sensitivity, a wide linear range, and low detection limit for electrochemical detection of urea.     

原位聚合法制备无机纳米粒子/聚合物复合材料的研究进展

介绍了无机纳米粒子/聚合物复合材料的制备方法与原位聚合法制备工艺。重点介绍了原位聚合法制备蒙脱土、纳米SiO2、纳米TiO2和纳米CaCO3等纳米复合材料的研究进展。     

Development of a POA/DBS/GOx Biosensor for the Determination of Glucose

In the present work, a simple technique is described for constructing a poly(o-anisidine) (POA)-dodecylbenzene sulphonic acid (DBS)-glucose oxidase (GOx) (POA-DBS-GOx) electrode. The enzyme glucose oxidase (GOx) was immobilized by crosslinking via glutaraldehyde on the POA-DBS film. The POA-DBS films were synthesized electrochemically on platinum substrate. The synthesized films were characterized by using electrochemical technique, conductivity measurement, UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The conductivity of the polymer films was found to be about 7.61 × 10^?2 S/cm. The crosslinking of enzyme and the porous morphology of the polymer film lead to good stability and good response time of the enzyme electrode. The stability and lifetime of the POA-DBS-GOx electrode have been studied. It shows very good stability and response for 3–4 weeks at 4°C. The results of this study reveal that a phosphate buffer gives better response than acetate buffer in amperometric measurements.     

聚合物/蒙脱石纳米复合材料研究进展

综述了蒙脱石的结构以及聚合物/蒙脱石纳米复合材料的制备原理与方法,重点介绍了纳米复合材料的制备方法与应用前景.     

胆固醇合成抑止剂的研究开发

综述了胆固醇生物合成过程及生物合成过程中关键酶抑制的研究开发现状。     

金纳米颗粒增强一次性使用葡萄糖传感器的响应性能

为了提高一次性使用葡萄糖传感器响应灵敏度,降低工作电压,研究了加入金纳米颗粒的一次性使用电化学葡萄糖传感器的响应性能。采用水溶性高分子材料聚丙烯酰胺包埋葡萄糖氧化酶(GOD)制备酶电极,研究了聚丙烯酰胺溶液浓度、纳米颗粒加入以及加入量等对电极响应的影响。结果表明引入纳米粒子可显著增强葡萄糖传感器的响应灵敏度,明显缩短传感器的响应时间(<20s),降低传感器的工作电压(<0.4V)。     

不同方法制备聚氨酯/蒙脱土纳米复合材料的力学性能研究

采用3种不同制备方法获得了聚氢酯／蒙脱土（PU／MMT）纳米复合材料。通过对材料力学性能测试,发现制备方法对纳米复合材料的拉伸强度杀Ⅱ断裂伸长率有明显影响。蒙脱土和三羟基聚氧化丙烯醚（GP03）预研磨混合制备法所得的纳米复合材料呈现出较好的力学性能。当有机蒙脱土质量分数为2％时,其拉伸强度和断裂伸长率分别比纯聚氨酯材料提高丁30％-68％。     

生物传感器研究近况与前景

本文对构造生物传感器的主要元件及其方法的研究近况作一综述,并作前景展望。     

乳液法制备聚合物纳米复合材料研究进展

介绍了近年来国内外学者采用微乳液聚合、常规乳液聚合、种子乳液聚合 ,核壳乳液聚合、辐射乳液聚合、可聚合的乳化剂和聚合物乳化剂聚合等多种乳液聚合法制备聚合物纳米材料所作的研究工作。     

尼龙6/粘土纳米复合材料的性能研究进展

粘土是一种层状硅酸盐,以纳米尺寸分布在聚合物中能提高聚合物的各种性能,本文综述了尼龙6/粘土纳米复合材料在机械性能、热稳定性、阻隔性能、结晶性能和流变行为等与纯尼龙6性能相比的优越性,同时详细地论述了性能得到改善的机理。     

原位乳液插层法制备丁腈橡胶/蒙脱土纳米复合材料的研究

结合乳液插层蒙脱土的特点，利用原位插层制备了丁腈橡胶（NBR）／改性蒙脱土（HOMMT）纳米复合材料。通过X-射线衍射（XRD）及透射电镜（TEM）证实和分析了蒙脱土的纳米分散相结构。物理性能的研究表明，所制备的复合材料体现了纳米材料的一些特点，填充少量蒙脱土，复合材料的物理机械性能即达到一个较高的水平。     

乳液聚合法制备PMMA/石墨烯纳米复合材料

首先以甲基丙烯酸二甲氨基乙酯（DMAEMA）为分散助剂得到石墨烯/MMA分散液,然后采用乳液聚合法制备了PMMA/石墨烯纳米复合材料。通过傅里叶变换红外光谱仪、拉曼光谱仪、扫描电子显微镜、透射电子显微镜、差示扫描量热仪、热重分析仪以及电子万能试验机、冲击试验机、高阻计等仪器设备对PMMA/石墨烯纳米复合材料的结构与性能进行分析和测试。结果表明,通过DMAEMA的助分散作用,实现了PMMA对石墨烯的完全包覆,并且DMAEMA的胺基与石墨烯表层官能团间存在强相互作用;石墨烯的引入提高了PMMA/石墨烯纳米复合材料的热稳定性,玻璃化转变温度（Tg）增加约6.4 ℃、初始热分解温度增加约38.3 ℃;石墨烯的引入改善了PMMA/石墨烯纳米复合材料的抗静电性能及拉伸性能,但冲击性能略有下降。