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The increase in cytosolic free Ca2+ concentration ([Ca2+]i) seen in submandibular cells of early postnatal rats following exposure to acetylcholine (ACh) is larger than in cells of adult rats. To elucidate possible reasons for this difference, we compared Ca2+ movements through Ca2+ pumps in both types of cells using Ca(2+)-sensitive fluorescent probe fura-2 and the radiotracer 45Ca2+. Ca2+ release induced by endoplasmic reticulum (ER) Ca(2+)-pump inhibitor thapsigargin (TG) was significantly smaller in neonatal cells than in adult cells, whereas the inositol 1,4,5-trisphosphate (IP3)-elicited Ca2+ release was comparable in both cell types. This suggests that although the size of the IP3-sensitive Ca2+ pool is adequate in immature cells, the activity of TG-sensitive Ca2+ pump in this pool is lower. The activity of the plasma membrane (PM) Ca(2+)-pump, measured by extrusion of 45Ca2+, was also significantly lower in immature cells. These results indicate that both ER and PM Ca2+ pumps may be functionally underdeveloped in immature cells, and that the enhanced increase of [Ca2+]i seen in response to ACh in immature cells may be partially, if not completely, due to a reduced capacity for removal of Ca2+ from the cytosol by active mechanisms.  相似文献   

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Conducted a study with 57 Ss (mean age, 6.78 yrs) to assess their conservation and transitivity of number, length, liquid amount, and weight. The conservation deduction argues that given A = B, B is transformed to B-sup-1, and that Ss somehow know that B-sup-1 = B as well as the concept of transitivity of equality Ss; thus should conclude that A must also equal B-sup-1, which is the conservation deduction. Results show that most Ss gave correct transitivity judgments, but only half gave correct justifications. In contrast, all the conservers gave good reasons. Overall conservation and transitivity correlated, and conservers were more likely to give good transitivity reasons than nonconservers. Nearly all conservers and about half the nonconservers saw their conservation judgments as necessary; thus, the relationships between conservation and necessity for number, length, amount, and weight were significant. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   

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镀锡板ATC值与合金层之间的关系   总被引:12,自引:0,他引:12  
比较了6种国内外食品罐用镀锡板的锡-铁合金层厚度和合金-锡电偶值(ATC值)。指出ATC值不仅与合金层厚度相关,更重要的是与合金层的连续性和致性有关。  相似文献   

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