A chiral dinuclear zinc complex can effectively catalyse the direct aldol reactions of pyruvic acid ester with various chiral sugar aldehydes, thus functionally mimicking the pyruvate‐dependent type II aldolases. Application of sterically hindered aryl esters allows for the elusive aldol reaction of the pyruvate donor with controlled anti‐selectivity en route to the short and efficient synthesis of 3‐deoxy‐2‐ulosonic acids. Pyruvic acid ester is here used as a chemical equivalent of phosphoenol pyruvate (PEP) in imitation of the synthetic principle used in nature. The presented biomimetic methodologies use enol formation for the highly efficient and flexible formation of various C6–C9 ulosonic acids. Particularly, efficient and concise syntheses of 3‐deoxy‐D ‐erythro‐hex‐2‐ulosonic acid (KDG, overall 50% yield), 3‐deoxy‐D ‐ribo‐hept‐2‐ulosonic acid (DRH, overall 53% yield) and 3‐deoxy‐D ‐glycero‐D ‐talo‐non‐2‐ulosonic acid (4‐epi‐KDN, overall 78% yield) are described. This direct efficient application of pyruvic esters does not require additional demasking steps and thus surpassess previously methodologies utilising masked pyruvic synthons such 2‐acetylthiazole and pyruvic aldehyde dimethyl acetal.
The mono- and bi-polar electrodialysis processes for pyruvate recovery and pyruvic acid generation, respectively, were examined in order to determine their feasibility for application in the pyruvate production process. Under optimum process conditions (constant current mode i = 39.7 A m−2, pyruvate model solution cp = 50 g dm−3 in the monopolar electrodialysis experiment pyruvate flux reached 367 g m−2 h−1 and specific energy consumption was 1.4 kWh kg−1. In the bi-polar electrodialysis experiment under optimum process conditions (constant current mode i = 9.6 A m−2, pyruvate model solution cp = 48 g dm−3, pyruvate flux reached 125 g m−2 h−1 and specific energy consumption was 1.5 kWh kg-1. Generally speaking, performances of the bi-polar electrodialysis were equal to the best process conditions observed with mono-polar electrodialysis. On the other hand, current densities investigated in the bi-polar electrodialysis experiments were four-fold lower than those applied in the mono-polar electrodialysis experiments. Additionally, a mathematical model to represent the ion and water transport behavior of an electrodialysis process for concentrating pyruvic acid under the influence of different current density was developed. Estimation of both the model parameters and model validation were demonstrated in the work. 相似文献
Slices of rat jejunum were incubated with [2-14C]pyruvate, [1-14C]acetate, or [3H]H2O to determine lipogenic activity. Under all conditions studied, pyruvate acted as a better precursor than acetate for fatty
acid synthesis but not for the synthesis of sterol. Exogenous glucose significantly (P≤0.05) increased the conversion of both
pyruvate and acetate to fatty acids. By contrast, fasting resulted in a decrease (p≤0.05) in lipogenic activity. The highest
levels of lipogenesis were observed when [3H]H2O + glucose at a concentration of 20 mM was used. From such experiments, the absolute rate of fatty acid synthesis in the
tissue preparation was calculated: 734±54 nmoles acetyl units incorporated into fatty acids/g tissue/hr. 相似文献
以二苯醚与丙酮酸乙酯为原料,经傅克酰基化一步合成恶唑菌酮关键中间体2-(4-苯氧基苯基)乳酸乙酯,考察了溶剂、投料比、AlCl3用量、缚酸剂、温度对收率的影响。-25℃,CH2Cl2为溶剂,NaHCO3为缚酸剂,n(二苯醚)∶n(丙酮酸乙酯)∶n(AlCl3)=1.4∶1.0∶1.2,2-(4-苯氧基苯基)乳酸乙酯收率86.2%,目标产物结构经IR、1 H NMR确证。 相似文献
1-[(2-Vinyloxy)ethoxy]ethyl acetate (1) was prepared by the addition reaction between ethylene glycol divinyl ether and acetic acid. 1 contains both a cationically polymerizable C=C double bond and a dormant initiating moiety for cationic polymerization. It
can, therefore, undergo self condensing cationic polymerization in the presence of a Lewis acid activator, such as zinc chloride.
Using this procedure, a novel dendritic polymer consisting of vinyl ether was prepared and its hyperbranched molecular structure
confirmed by FT-IR and 1H NMR spectra.
Received: 22 May 1997/Revised: 17 July 1997/Accepted: 6 August 1997 相似文献
A resting cell system was developed for bacteriocin Lac-B23 production from Lactobacillus paracasei J23. The resting cell medium contained (g/L): Glucose 20, Sodium acetate 5.0, MnSO4 0.25 MgSO4 0.5, Ammoniumhydrogencitrate 1.0, KH2PO4 1.0. The resting cell incubation time and temperature were 20 h and 37 °C and the effects of exogenous factors, including amino acids, glycerol, pyruvic acid, and α-ketoglutaric acid were investigated. Cys and Gly could stimulate the production of bacteriocin, while no stimulus effect was observed for Glu, Tyr and Ala. Glycerol and pyruvic acid increased bacteriocin production and the optimum concentrations were 1% and 30 g/L, respectively. Bacteriocin could act as an inducer of its own biosynthesis. These findings are of importance for the further study of bacteriocin biosynthesis regulation and for the improvement of bacteriocin production yields. 相似文献