酶联免疫法检测克伦特罗残留量的质量控制

对酶联免疫法检测克伦特罗残留量的质量控制方法进行了探讨.应用酶联免疫法检测克伦特罗残留量可从克伦特罗试剂盒的检测下限、校正曲线的线性检验、精密度验证、以及添加克伦特罗标准品做回收率试验等方面来进行质量控制.结果表明,本方法的样品检测下限(定性检出)为0.03ng/mL,定量检测下限为0.1ng/mL;克伦特罗标准校正曲线Y=-0.1884X 0.4159在0.1～8.1ng/mL范围内具有良好的线性相关关系,相关系数为-0.9913;克伦特罗标准液的浓度分别为0.1、0.3、0.9、2.7、8.1ng/mL时,变异系数分别为3.7%、5.3%、9.7%、9.2%、8.7%,在3.7%～9.7%的范围内;当克伦特罗标准品添加水平分别为0.5、1.0ng/mL时,平均回收率分别为84%、90%,在80%～100%的范围内.上述指标均符合残留分析质量控制的要求.     

对胶体金免疫层析法定量检测猪肉中克伦特罗的几点分析

克伦特罗是常见β-肾上腺素受体激动剂成分,在畜牧生产中常作为饲料添加剂使用,对动物体内代谢途径产生调节作用,具有加速肌肉生长的效果。但人长期使用含克伦特罗食品,可能出现乏力、头晕、心悸等中毒症状,严重时可能危及生命。目前,欧盟各国以及我国等国家地区已经明确禁止克伦特罗用于动物饲料添加剂,规定在动物类食品中不得检出克伦特罗残留。在有关动物组织样品克伦特罗添加检测方法中,胶体金免疫层析法具有检测速度快、操作简单的优势,且抗基质干扰能力强,在基层现场筛查中有较好的应用价值。本文即尝试应用胶体金免疫层析法对猪肉中克伦特罗进行定量检测,对检测方法以及具体结果进行分析,通过试验结果验证了胶体金免疫层析定量检测猪肉中克伦特罗残留的可行性。     

出口肉及制品中盐酸克伦特罗的ELISA检测方法的建立

介绍了酶联免疫吸附法测定出口肉及制品中的盐酸克伦特罗的方法。利用盐酸克伦特罗试剂盒对出口肉及制品中残留的盐酸克伦特罗用酶标仪进行测定分析,结果显示,盐酸克伦特罗的交叉反应为100%,猪肉罐头和牛肉中的检测限(LOD)为0.026(μg/kg)和0.028(μg/kg),样品的平均回收率在83.6%～91.3%之间,相对标准偏差为3.5%～5.6%,经HPLC确证假阳性率≤2.0%。表明酶联免疫分析定量法可快速、准确实现对食品中盐酸克伦特罗残留量的快速筛选。     

竞争酶联免疫吸附法测定猪肉中的瘦肉精

介绍了竞争酶联免疫吸附法测定猪肉中的盐酸克伦特罗的方法.利用盐酸克伦特罗试剂盒,对猪肉组织中残留的盐酸克伦特罗经抽提、竞争后,用酶标仪进行检测分析.此法较适用于现场检验,检测速度快、灵敏度高,是保证肉品卫生安全的较好监控方法.     

快速检测卡和酶联免疫试剂盒检测动物性食品中3种β-兴奋剂

目的评价快速检测卡和酶联免疫试剂盒检测动物性食品中3种β-兴奋剂的准确性。方法分别采用快速检测卡和酶联免疫吸附法检测动物性食品中盐酸克伦特罗(CLB)、莱克多巴胺(RAC)和沙丁胺醇(SAL)3种β-兴奋剂残留,并与液相色谱-串联质谱法(LC-MS/MS)进行比较评价。结果盐酸克伦特罗速测卡和克伦特罗-莱克多巴胺-沙丁胺醇三联速测卡的检测结果与LC-MS/MS的符合率分别为83.3%和87.5%(CLB)、12.5%(RAC)、100%(SAL),CLB、RAC、SAL和β-兴奋剂ELISA试剂盒的检测结果与液相色谱-串联质谱法的符合率分别为94%、94%、76.2%和75%。结论试剂盒的准确度相对较高,但部分试剂盒的假阳性和假阴性情况明显。速测卡检测限较高,准确度较差,不满足一般检测要求。     

竞争酶联免疫吸附法测定猪肉中的盐酸克伦特罗的研究

介绍了竞争酶联免疫吸附法测定猪肉中的盐酸克伦特罗的方法。利用盐酸克伦特罗试剂盒，对猪肉组织中的盐酸克伦特罗残留经抽提、竞争后，用酶标仪进行检测分析。此法较适用于现场检验，检测速度快、灵敏度高，是保证肉品卫生安全的较好监控方法。     

克伦特罗残留量的检测方法

研究了应用酶免疫反应(ELISA)方法检测肉品中克伦特罗(Clenbuterol)残留量的制样和运用德国R—Biopharm公司克伦特罗残留测定试剂盒测定的具体操作方法及注意事项，可将克伦特罗检测下限达到0．1μg/kg。     

北京赛恩思科贸有限公司食品检测试剂盒

β -兴奋剂 (盐酸克伦特罗 )ＥＬＩＳＡ检测试剂盒 ,测定简单 ,方便 ,快速 ,灵敏度可达到 0 1ｐｐｂ ,适合各级卫生防疫部门使用。德国拜发公司ｒ Ｂｉｏｐｈａｒｍ检测盒霉菌毒素ＥＬＩＳＡ检测试剂盒黄曲霉毒素Ｂ1 　ＡｆｌａｔｏｘｉｎＢ1黄曲霉毒素总量　Ａｆｌａｔｏｘｉ     

应用ELISE法测定盐酸克伦特罗的残留

介绍了酶联免疫吸附法测定猪肉中的盐酸克伦特罗的方法.利用试剂盒对猪肉中的盐酸克伦特罗残留处理后,用酶标仪进行检测分析.此法较适用于现场检验,检测速度快、灵敏度高,是保证肉品卫生安全的较好监控方法.     

多厂家商品化黄曲霉毒素M1检测试剂盒质量评价与分析

目的对市场上11个厂家商品化黄曲霉毒素M_1快速检测试剂盒进行质量评价。方法定性检测试剂盒验证其灵敏度、特异性、假阳性率、假阴性率、符合率;定量试剂盒验证其范围和线性、准确度、精密度,并与实验室参考方法比较,分别对定性、定量试剂盒进行质量评价。结果 9个厂家定性试剂盒灵敏度、特异性及符合率均为100%,假阳性率及假阴性率均为0;厂家编号10的定量试剂盒准确度和精密度较差,厂家编号11的定量试剂盒线性、准确度及精密度较好,可以满足检测需求。结论黄曲霉毒素M_1快速检测试剂盒在定性检测方面较成熟,能够参与市场监督;定量试剂盒产品不够成熟,使用前应严格验证其指标。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Chlorohydrins of bisphenol A diglycidyl ether (BADGE) and of bisphenol F diglycidyl ether (BFDGE) in canned foods and ready-to-drink coffees from the Japanese market

BADGE.2HCl and BFDGE.2HCl were determined in 28 samples of ready-to-drink canned coffee and 18 samples of canned vegetables (10 corn, 5 tomatoes and 3 others), all from the Japanese market. HPLC was used as the principal analytical method and GCMS for confirmation of relevant LC fractions. BADGE.2HCl was found to be present in one canned coffee and five samples of corn, BFDGE.2HCl in four samples of canned tomatoes and in one canned corn. No sample was found which exceeded the 1mg/kg limit of the EU for the BADGE chlorohydrins. However the highest concentration was found for the sum of BFDGE.2HCl and BFDGE.HCl.H₂O at a level of 1.5mg/kg. A Beilstein test confirmed that all cans containing foods contaminated with BADGE.2HCl or BFDGE.2HCl had at lest one part coated with a PVC organosol.     

European priorities for research to support legislation in the area of food contact materials and articles

A strong science base is required to underpin the planning and decision-making process involved in determining future European community legislation on materials and articles in contact with food. Significant progress has been made in the past 5 years in European funded work in this area, with many developments contributing to a much better understanding of the migration process, and better and simpler approaches to food control. In this paper this progress is reviewed against previously identified work-areas (identified in 1994) and conclusions are reached about future requirements for R&D to support legislation on food contact materials and articles over the next 5 or so years.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the