大豆醇溶性磷脂制备中浸提温度对产品指标的影响

考察了大豆醇溶性磷脂制备中,浸提温度对醇溶性磷脂产品酸值、过氧化值、磷含量及氮含量的影响。结果显示,采用95%乙醇作为浸提溶剂时,其浸提温度不宜太高,浸提温度最好控制在40℃,结合氮气驱氧,能较好地控制大豆醇溶性磷脂的品质指标。      

氧化铝吸附醇溶性磷脂中磷脂酰乙醇胺的动力学研究

研究氧化铝吸附醇溶性磷脂中磷脂酰乙醇胺的动力学行为.结果表明:氧化铝吸附磷脂酰乙醇胺的吸附速率曲线表现为一级动力学特征;用孔扩散方程对氧化铝吸附磷脂酰乙醇胺的吸附速率曲线进行拟合,各相关系数都大于0.99,表明孔扩散是该吸附过程的主要控制步骤,但由于其拟合直线都不经过原点,表明它不是唯一的控制因素.所以,认为膜表面扩散与孔扩散共同影响氧化铝对磷脂酰乙醇胺的吸附过程.     

氧化铝吸附醇溶性磷脂中磷脂酰乙醇胺的热力学研究

研究了氧化铝吸附醇溶性磷脂中磷脂酰乙醇胺的热力学行为,结果表明,三氧化二铝对磷脂酰乙醇胺的吸附符合Langmuir方程;三氧化二铝对磷脂酰乙醇胺的吸附为放热吸附,低温有利于吸附;三氧化二铝对磷脂酰乙醇胺的吸附为物理吸附过程,以上研究对磷脂单体的制备具有指导意义.     

氧化铝吸附醇溶性磷脂中磷脂酰乙醇胺的热力学研究

研究了氧化铝吸附醇溶性磷脂中磷脂酰乙醇胺的热力学行为,结果表明,三氧化二铝对磷脂酰乙醇胺的吸附符合Langmuir方程;三氧化二铝对磷脂酰乙醇胺的吸附为放热吸附,低温有利于吸附;三氧化二铝对磷脂酰乙醇胺的吸附为物理吸附过程,以上研究对磷脂单体的制备具有指导意义。      

分散剂对醇溶性磷脂浸出的动力学研究

针对含水醇类、较高温度和较小料液比时以粉末磷脂为原料浸出醇溶性磷脂,浸出过程中常有磷脂分散不好的情况,采用分散剂分散粉末磷脂来提高醇溶性磷脂的传质效率,考察了不经分散剂分散和经分散剂分散的磷脂浸出动力学行为,并分别得到了它们的动力学方程:-dq/dt=0.00719e -0.055t(不加分散剂),-dq/dt=0.00491e -0.0742t(砂-100为分散剂),-dq/dt=0.02208e-0.0522t(糊精为分散剂).研究结果表明,经分散剂分散的磷脂可大大提高醇溶性磷脂的浸出速率和有效浸出时间.     

磷脂产品的常用法规、术语、指标

介绍了有关组织对磷脂的法规、术语、指标及意义.     

磷脂产品的常用法规、术语、指标

介绍了有关组织对磷脂的法规、术语、指标及意义。     

醇浓度对醇法大豆浓缩蛋白浸提液中溶出蛋白的影响

利用不同浓度乙醇对低变性脱脂大豆粕进行醇法浸提，制取大豆浓缩蛋白。对浸提液采用SDS—PAGE电泳法测定蛋白质分子量，并对蛋白质溶出率进行测定，探索不同醇浓度对浸提液中蛋白溶出率及分子量的影响，为醇法制取大豆浓缩蛋白浸提液再加工、回收蛋白提供了一定的理论基础。     

醇法大豆浓缩蛋白浸提工艺研究

主要介绍了乙醇浸提法生产大豆浓缩蛋白的浸提工艺。探讨了主要工艺参数:乙醇浓度、浸提时间、浸提温度对整个浸提工艺的影响。通过正交试验确定了醇法生产大豆浓缩蛋白的最佳浸提工艺条件为:乙醇浓度70%,浸提时间90 m in,浸提温度50℃,在此条件下生产的大豆浓缩蛋白其蛋白含量可达到75.48%。     

大豆溶血磷脂的制备及其对面包品质的影响

利用液态磷脂酶A1,在水相中催化水解大豆粉末磷脂制备溶血磷脂,得出最佳反应条件为:磷脂与溶剂比为15%、加酶量6%、pH=5.0、反应温度50℃、反应时问8h,此条件下反应得磷脂酸值为79.46mg KOH/g.并研究了溶血磷脂对面包焙烤品质的影响,发现溶血磷脂的用量在0.60%～1.0%范围内能有效增大面包体积;硬度值测定结果表明,溶血磷脂对延缓面包老化有显著效果.     

花生磷脂制取工艺的研究

对花生磷脂的制取工艺进行研究,研制出高等级的花生磷脂产品.花生油的生产采用静电处理和冷冻过滤等工艺,得到质量较好的、不含水的毛磷脂胶质副产品,采用超临界萃取技术对毛磷脂进行提纯,利用二氧化碳在临界高压下对非极性的油类具有溶解性而对极性的磷脂难以溶解的特性,达到将磷脂从含油胶质中提取分离的目的.磷脂产品外观为淡黄色,磷脂质含量大于90%.     

Effect of low temperature upon vitality of Saccharomyces cerevisiae phospholipid mutants

The phospholipid metabolism of Saccharomyces cerevisiae plays a central role in its adaptation to low temperatures. In order to detect the key genes in this adaptation, various phospholipid mutants from the EUROSCARF collection of Saccharomyces cerevisiae BY4742 were tested to ascertain whether the suppression of some genes could improve the fermentation vitality of the cells at low temperature. The cell vitality and phospholipid composition of these mutants were analysed. Some knockouts improved (hmn1Δ) or impaired (cho2Δ and psd1Δ) their vitality at low temperature (13 °C) but were not affected at optimum temperature (25 °C). A common trait of the mutants that had some defect in vitality was a lower concentration of phosphatidylcholine and/or phosphatidylethanolamine. The supplementation with choline allowed them to recover viability, probably by synthesis through the Kennedy pathway. Hmn1Δ showed a lower concentration of phosphatidylcholine, which explains the dominant role of the de novo pathway in cellular phosphatidylethanolamine and phosphatidylcholine vs the Kennedy pathway. The absence of such genes as CRD1 or OPI3 produced important changes in phospholipid composition. Cardiolipin was not detected in crd1Δ but phosphatidylglycerol circumvents most of the functions assigned to CL. The considerable reduction in PC diminished the cell vitality of opi3Δ at both temperatures, although the decrease at 13 °C was more marked. Copyright © 2012 John Wiley & Sons, Ltd.     

温度和pH对儿茶素萃取平衡的影响

采用摇瓶-分光光度法测定不同温度和不同pH值下茶多酚在乙酸乙酯/水中的分配系数。结果表明,温度和pH对儿茶素在萃取体系中的分配系数有显著影响,温度为285.13K和pH4.6时茶多酚在乙酸乙酯中的分配系数为6.976,是313.15K和pH6.0时的2.23倍,因此低温和pH4.6环境有利于茶多酚的萃取。     

改性大豆磷脂在脂质体制备中的应用研究

以大豆磷脂和聚乙二醇单甲醚2000为原料,制备了大豆磷脂酰乙醇胺-聚乙二醇单甲醚2000(SPE-MPEG2000),产物结构经1H NMR和IR进行了表征。采用薄膜分散法制备了含SPEMPEG2000的空间稳定脂质体(SSL),同时制备不含SPE-MPEG2000的普通脂质体(CLs)。通过TEM考察了两种脂质体的形态及粒度分布,测定了粒径及Zeta电位变化,采用紫外分光光度法测定了脂质体加乙醇后的吸光度变化及包封钙黄绿素后的包封率。结果表明:SSL粒子呈类球形,粒径分布较CLs均一且分散性较好;放置前后SSL的粒径及吸光度变化较小,包封率高于CLs,从而说明SPE-MPEG2000对脂质体有良好的稳定作用。     

超声辅助提取技术在天然产物提取中的应用

简述超声辅助提取技术提高活性成分提取率的作用及原理,对超声辅助提取技术在天然产物有效成分(生物碱、黄酮、蒽醌、皂甙、植物油)提取方面,以及在农残分析样品提取方面的应用研究成果进行概述,并指出当前研究中存在的问题和今后的研究方向。     

鱼油提取物磷脂片制剂工艺研究

目的　研究鱼油及提取物片配方及制剂方法。方法　通过筛选辅料、填充剂、崩解剂、吸附剂等开展鱼油及提取物片成型工艺研究。结果　通过对比试验分析出优选磷酸氢钙、微晶纤维素、二氧化硅等为主要辅料,可提高产品可压性及崩解效果。经检测片子硬度可达到17~20 kg/cm2,检测产品的脆碎度＜0.5%。符合产品质量,生产工艺可操作性强。结论　本文优选的制剂方法稳定、可行为鱼油及提取物片制备工艺的确定及生产的控制提供了科学的实验依据。同时为油脂类物料制成片剂的制备工艺提供参考依据。     

Effect of the temperature of extraction on the composition of cell wall polysaccharides in olives

A comparative study of methods for isolating cell wall material from olives (variety Hojiblanca) was performed. Treatment with cold (T-1) or hot (T-2) ethyl alcohol was followed by extraction into cold or hot water (water-soluble fraction). The insoluble residues were delignified. Hemicelluloses A and B were isolated and the neutral sugar composition and protein content of the different fractions were determined. In the delignified fractions following treatments T-1 and T-2, hemicelluloses A were composed of 87.35% and 87.37% xylose, respectively, indicating the presence of a pure xylan. In the non-delignified fraction, the content of hemicelluloses B was higher than in the delignified fraction and its predominant sugar was glucose, although significant amounts of xylose, galactose and arabinose were also found. The study suggests that treatment T-1 gives better results than T-2 and that a higher degree of purification of hemicelluloses A is obtained from the delignified cell wall material, as well as a decrease in the proteins bound to the fractions.     

Effect of ultrasonication on the immuno-stimulatory activity of Angelica gigas Nakai in low temperature extraction

The biological activities of Angelica gigas Nakai can be improved using water extraction at 60°C with ultra-sonication. Conventional water extraction at 100°C can destroy heat labile active compounds in medicinal herbs. A high frequency (200 kHz) (actual intensity, 210 W/m²) minimally affected the extraction yield at 40°C. A low frequency (40 kHz) (50 W/m²) at 60°C produced better results. A. gigas Nakai extracts produced using a low frequency showed a 50% increase in human immune cell growth and IL-6 secretion, compared to conventional water extraction extracts. Extracts produced using 40 kHz and 60°C produced more immune B and T cell growth than 40°C extracts. These results were confirmed using similar HPLC chromatogram peak patterns at input intensities of 50 and 210 W/m². A better than 50% extraction yield and cytokine secretion increase, and better immune cell growth, was achieved in ultrasonication extracts produced at low temperatures.     

超声波技术在天然产物提取中的应用

简述了超声波作用的基本原理，综述了超声波技术在天然产物活性物质提取中的应用情况，得出了超声波技术提取天然产物活性物质有着提取率高、速度快且不改变有效成分等优点。讨论了超声波提取中应注意的问题。     

超声波技术在天然产物提取中的应用

简述了超声波作用的基本原理,综述了超声波技术在天然产物活性物质提取中的应用情况,得出了超声波技术提取天然产物活性物质有着提取率高、速度快且不改变有效成分等优点。讨论了超声波提取中应注意的问题。