共查询到19条相似文献,搜索用时 63 毫秒
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目的优化猪源枯草芽孢杆菌喷雾干燥工艺,提高菌体喷雾干燥后的存活率。方法应用SAS V 8.0统计分析软件中的二次正交旋转组合设计方案进行试验设计,分析各因素对Y值的效应关系;应用优化的喷雾干燥工艺进行3次重复喷雾干燥试验,验证预测结果的准确性;并检测芽孢率对菌体喷雾干燥存活率的影响。结果优化的喷雾干燥工艺中各条件的最佳参数为:出口温度90℃,入口温度150℃,保护剂浓度20%,入料速度1 200 ml/h;应用优化的喷雾干燥工艺进行3次重复喷雾干燥试验,菌体平均存活率为75.7%,与预测值(77%)基本相符;芽孢率对菌体喷雾干燥存活率影响较大。结论优化了猪源枯草芽孢杆菌的喷雾干燥工艺,为其工业化生产奠定了基础。 相似文献
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《应用化工》2022,(12)
对一株适用于采油的枯草芽孢杆菌发酵培养基组成和发酵条件进行了优化。通过实验室内的摇床发酵,得到优化后培养基为:蔗糖50 g/L,NaNO_3 3.4 g/L,NH_4Cl 1.1 g, KH_2PO_4 2.5 g/L,12H_2O·Na_2HPO_4 30 g/L,7H_2O·MgSO_4 0.8 g/L,脂肽平均产量为4.2 g/L,表面张力可降低至25.15 mN/m;同时,因为在采油工业中,每吨NH_4Cl比每吨NaNO_3的成本低30%左右,所以通过NH_4Cl的加入,减少了NaNO_3的用量,大大降低了发酵成本;最佳培养条件为:温度37℃,装液量150 mL/250 mL,发酵时间100 h。 相似文献
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目的优化重组干酪乳杆菌喷雾干燥制剂的制备工艺。方法以乳清蛋白为悬浮基质与重组干酪乳杆菌混匀,进行喷雾干燥,通过单因素试验确定乳清蛋白的添加量(10%、20%、30%、40%、50%、60%、70%、80%、90%)及进风温度(60、80、100、120、140、160、180℃)、给气量(65%、70%、75%、80%、85%、90%、95%、100%)和进料速度(2.5、5.0、7.5、10.0、12.5、15.0 mL/min)等喷雾干燥工艺参数;以活菌数和存活率为指标,通过正交试验进一步优化工艺参数。同时检测最适工艺制备的重组干酪乳杆菌喷雾干燥制剂的质量指标和贮藏稳定性。结果最适乳清蛋白添加量为20%,最适工艺参数:进风温度为120℃,给气量为75%,进料速度为10 mL/min。喷雾干燥制剂相关的质量指标均符合要求,在4℃真空条件下贮藏稳定性最好。结论优化了重组干酪乳杆菌喷雾干燥制剂的制备工艺,为其产业化生产奠定了基础。 相似文献
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通过单因素实验和响应面实验对解淀粉芽孢杆菌(Bacillus amyloliquefaciens) BSK532的发酵条件进行了优化。确定最佳发酵条件为:碳源淀粉浓度41.36 g·L-1、氮源蛋白胨浓度26.13 g·L-1、pH值7.24、装液量75 mL/500 mL,在此条件下,芽孢数达到195.33×108 CFU·mL-1,较优化前提高了94.36%;优化后发酵液的粒径分布范围为0~10μm,D90为1.565μm,较优化前(粒径分布范围为0~200μm,D90为95.44μm)大幅减小,减小发酵液粒径可以防止大疆T20植保无人机喷头堵塞;采用平板对峙法测定了优化前、后解淀粉芽孢杆菌BSK532发酵液对6种常见农作物病原菌的抑菌带宽度,优化后的抑菌带宽度较优化前增加了50%~60%,表明提高芽孢数可以增强抑菌作用。为解淀粉芽孢杆菌制剂的开发应用提供了一定的理论基础。 相似文献
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采用正交实验设计L9(3^4)方法,利用摇瓶培养研究了产氨基酰化酶的米曲霉的培养温度、培养基pH和培养时间3个因素对菌体酶活的影响。结果表明,在培养温度29℃,培养基起始pH6.5以及培养时间50h。其酶活达595U/g以上。采用正交试验设计可以大大减小工作量,是一种高效率、快速、经济的实验设计方法。 相似文献
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地衣芽孢杆菌SY-8具有分泌β-甘露聚糖酶的能力.为提高地衣芽孢杆菌SY-8的产酶能力,必须先找到该菌培养的最佳环境条件,因此考查温度、摇床转速、传代浓度、培养pH、渗透压等因素对细胞生长状态的影响,发现当温度为30℃,摇床转速为100 rpm/min,传代浓度为1.25×106 cell/mL,细胞培养pH为6.5,渗透压为360 osmol/g时,能达到SY-8细胞培养的最佳条件,有利于满足大规模培养的需要. 相似文献
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采用液体培养法,将巨大芽孢杆菌、枯草芽孢杆菌和解淀粉芽孢杆菌应用于聚乳酸生物降解,探究降解过程中培养条件的改变,对蛋白酶活性以及薄膜降解过程的影响。结果表明:不同种类的诱导物能够提高芽孢杆菌的蛋白酶活性,巨大芽孢杆菌的最高蛋白酶活性约为23.79 U/mL,枯草芽孢杆菌的最高蛋白酶活性约为11.61 U/mL,解淀粉芽孢杆菌的最高蛋白酶活性约为21.23 U/mL。三种芽孢杆菌中,巨大芽孢杆菌对聚乳酸的生物降解影响最大。初始pH值为8.0,接种2%(V/V)种子液,1%酵母浸粉作为降解诱导物,可以有效加快巨大芽孢杆菌对聚乳酸的降解速率,5 d后降解率可达20.96%。薄膜表面存在菌生长,生物降解后出现轻微裂痕。 相似文献
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用正交试验法对磷酸二氢钾料液喷雾干燥的工艺进行优选,以磷酸二氢钾粉末中的水分和干燥损失率为指标,选用L9(34)正交试验表进行正交试验。结果表明:磷酸二氢钾料液喷雾干燥的最佳工艺条件为料液浓度18%、进风温度180℃、进料量120mL/h和压缩空气流量10L/min。在此条件下,磷酸二氢钾损失率最低。 相似文献
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Elham Assadpour 《Drying Technology》2017,35(9):1152-1160
Research over recent decades has shown that inadequate folate concentrations may contribute to some disorders. Cereal flours have been a primary candidate for fortification by folic acid as they are consumed by most of the people but the main obstacle is destruction of this vitamin by the process and environmental deteriorating factors. So, it can be nano-encapsulated for protection and efficient delivery. Our main goal was to find the optimum conditions in spray drying of pectin–whey protein concentrate (WPC) double emulsions containing folic acid. Five independent variables including pectin and WPC content, dispersed phase content, pH, and surfactant type of span and polyglycerol polyricinoleate (PGPR) were considered along with water activity (aw) and bulk density as the main responses. The experiment design was performed by Taguchi approach and 16 treatments were determined. The grand average of aw and bulk density for folic acid powders was approximately 0.371 and 343?kg/m3, respectively. The main effect analysis with Taguchi technique revealed that pectin content was the most important factor affecting aw (39.5%), and WPC had the minimum influence (6%). In terms of bulk density, it was found that the most important parameter was WPC (29.6%) and the minimum influence was related to surfactant system (8.1%). Finally, optimum conditions were determined as 0.5% pectin, 4.0% WPC, 10% dispersed phase, and pH 8, with a PGPR micro-emulsion, which resulted in aw?=?0.229, and 1.5% pectin, 8.0% WPC, 10% dispersed phase, and pH 9, with a PGPR nano-emulsion, which resulted in density?=?245.45?kg/m3 for folic acid powders. 相似文献
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Jorge Castro-Albarrán Blanca Rosa Aguilar-Uscanga Frédéric Calon Isabelle St-Amour Josué Solís-Pacheco Linda Saucier 《Drying Technology》2016,34(15):1801-1809
Several freeze-drying and spray-drying methods were investigated in relation to the retention of immunoglobulins (Ig) A, IgG, and IgM. Spray drying produced human milk powders with 2% humidity and a good retention of IgG (>88%) and IgM (~70%). However, only 38% of IgA remained after spray drying. For freeze drying, only the highest heating plate temperature used in this study (40°C) brought IgA content down to 55% in powder with 1.75% residual humidity, whereas milk samples undergoing lower temperatures had higher preservation rates (75% for IgA and 80% for IgG and IgM) and higher residual moisture contents. From these results, it can be concluded that IgA is the most sensitive Ig lost during drying processing of human milk. The best method to generate human milk powders without a significant loss of Ig was thus freeze drying at 30°C heating plate temperature, which accelerated the process compared to lower processing temperatures, but still had good overall Ig retention. 相似文献
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