Effects of low dose oxymatrine on mouse lymphocyte proliferation stimulated by Con A

Objective To investigate the effects of low dose Oxymatrine (OMT) on mouse lymphocyte proliferation stimulated by Con A making use of fluorescence dyestuff CFDA-SE. Methods CFDA-SE staining and flow cytometry were used to detect the fluorescence intensity of lymphocytes after stimulated by polyclonal stimulators Con A and OMT. Then, related software was used to analyze the effects of OMT on mouse lymphocyte proliferation.Results After cultured for 48 h, CFSE fluorescence could be detected by cytometer, filial generation peaks did not appear in control group, which indicated that lymphocytes did not proliferate. Three peaks were obviously detected in Con A group which indicated that Lymphocytes divided after 48 h stimulated by Con A compared with the halving of the fluorescence intensity of control group. In groups with Con A and OMT treated, Primary generation peaks are all lower while filial generation peaks are significantly higher than groups with Con A treated only. This indicated OMT obviously promote lymphocyte proliferation. After cultured for 72 h, the fluorescence intensity changes between all groups are consistent with those of cultured for 48 h. Analyzed with CELLQuest software, it is shown that OMT could promote lymphocyte proliferation in 16, 8, 4 and 2μg/mL respectively. Conclusions 1) CFDA-SEdyeing and flow cytometer were both reliable tools to analyze lymphocyte proliferation; 2) lower dosage of OMTcould promote the proliferation of lymphocyte as a immunopotentiator.     

The Difference of Sensitivity between BXPC-3 and K562 Cells by Treatments with Combination of Indole-3-acetic Acid and Horseradish Peroxidase

The difference of sensitivity to indole- 3-acetic acid （ IAA ） combined with horseradish peroxidase （HRP） in K562 and BXPC- 3 cells was investigated. The cell proliferation was determined by MTF assay. The cell cycle and apoptosis of K562 and BXPC-3 cells were examined by a fluorescence flow cytometer （FCM） and terminal deoxynacleotidyl transferase-mediated dUTP nick-end labeling （TUNEL） respectively. The experimental results show that IAA and HRP could inhibit BXPC- 3 cell proliferation greatly compared with K562 cell during the first 48 h . The cell cycle was arrested predominantly at G2/ M phase in K562 and BXPC- 3 cells. The cell apoptosis of K562 and BXPC- 3 was induced by IAA/ HRP. There was a significant difference between the two cell lines since BXPC-3 cells were more sensitive than K562 cells by treatments with combination of IAA and HRP.     

Effect of a bone graft substitute β tricalcium phosphate on osteoblastic genes mRNA exprssion

To investigate the molecular aspects of osteoblastic interactions with β tricalcium phosphate (β-TCP) particles, human osteoblast-like MG-63 cells were cultured with β-TCP particles at a density of 6 mg/mL culture medium for 48 h. Then, the mRNA expression of selected genes were quantified by real-time polymerase chain reaction (PCR), including the attachment-related genes (α integrin and actin), the proliferation-related gene (c-jun), and the osteoblastic markers genes (type I collagen, osteonectin, alkaline phosphatase, RUNX2 and osteoclain). The results showed that β-TCP particles (the average size 809 nm) significantly promote the attachment and the proliferation of MG-63 cells, and slightly enhance the osteoblastic differentiation based on the analyses of the related genes expression. This study provided scientific evidences to better reveal the underlines of functions of β-TCP in bone repair.     

Fluorescence spectroscopy characteristics of humic acid by inoculating white-rot fungus during different phases of agricultural waste composting

The white-rot fungus, Phanerochaete chrysosporium (P. chrysosporium), was inoculated during different phases of agricultural waste composting, and its effect on the fluorescence spectroscopy characteristics of humic acid (HA) was studied. The results show that the emission spectra have a sharp peak at 400 nm and a broad shoulder with the maximum centered at 460 nm. The excitation spectra have two peaks and exhibit red shift (shift to longer wavelengths) at 470 nm. The synchronous scan spectra present a number of peaks and shoulders, and the peaks at shorter wavelengths disappear gradually and form a shoulder. At the final stage of composting, the fluorescence spectra have similar shapes, but the fluorescence intensities decrease. P. chrysosporium increases the degree of aromatization and polymerization of HA when it is inoculated during the second fermentation phase, while it does not produce an obvious change on the humification degree of HA when it is inoculated during the first fermentation phase. Compared with the fluorescence spectroscopy characteristics of HA from soil, the structure of HA from compost is simpler and the activity is higher. Foundation item: Project(2005CB724203) supported by the Major State Basic Research and Development Program of China; Project(IRT0719) supported by the Program for Changjiang Scholars and Innovative Research Team in University of China; Projects(50608029, 50808073) supported by the National Natural Science Foundation of China; Project(2007185) supported by the Environmental Protection Technology Research Program of Hunan Province, China     

Domain structure and defects of highly ordered Bi<Subscript>4</Subscript>Si<Subscript>3</Subscript>O<Subscript>12</Subscript> micro-crystals

Highly ordered Bi₄Si₃O₁₂ micro-crystals were prepared at normal atmosphere. Phase identification of the prepared crystals was accomplished by X-ray diffractometer (XRD). Domain structure and defects were characterized by environmental scanning electron microscopy (ESEM). XRD shows that the obtained micro-crystals are of eulytite structure with chemical formulation of Bi₄Si₃O₁₂. A highly ordered growth pattern is confirmed due to the faster growth of the {124} faces than that of the {204} faces by ESEM. The growing process of the domain structure is of pollen parent and filial generation pattern. The filial generations of Bi₄Si₃O₁₂ crystals are generated from the pollen parent. Cracks generate from the defect areas and propagate along the {124} faces due to their lower binding energy under a proper temperature gradient, contributing to the total transcrystalline fracture. It is confirmed that the generation and development of the voids in the crystal grains can be developed when unmatched dimensions of the two opposite faces are formed. And the development of the voids is dependent on the dimensions and orientations of the two opposite faces. Supported by the Innovation Research Team Funds of Shaanxi University of Science & Technology (Grant No. SUST-A04)     

The effect of OH~- groups on the spectroscopic properties of erbium-doped tellurite glasses

With the rapid development of computer networks and other data-transmitting ser-vices, the demand for the increase of transmission capacity of the long distance trans-mission system is urgent. However, the conventional SiO2-based EDFA is limited for its small bandwidth. The Er3+-doped tellurite glass exhibits a larger stimulated-emission cross section and a broader emission bandwidth at the third communication window (1.55 μm) than that of silicate, phosphate, and germanate glasses, which c…     

四种黑色珍珠特征对比

通过显微镜、紫外荧光、阴极发光特征的观察和拉曼光谱分析,对塔希提黑珍珠、γ射线辐照淡水养殖珍珠、不同工艺染色的淡水养殖黑珍珠和染色加辐照黑珍珠这4种黑色珍珠的特征进行了对比研究.结果表明:塔希提黑珍珠在长波紫外灯下可发暗红棕色荧光,短波紫外灯和阴极射线下不发光,拉曼光谱可见文石和有机卟啉峰;辐照处理淡水养殖珍珠和染色加辐照处理淡水养殖珍珠在长波紫外灯下和阴极射线下均发强绿色光,且能观察到不同程度的表面破损和内部龟裂纹,拉曼光谱只可见文石的谱峰;染色淡水养殖黑珍珠在紫外灯下荧光不强,在阴极射线下发黄绿色光,可见表面破损.     

Effect of heparin on high glucose induced proliferation and expression of matrix metalloproteinases in normal human mesangial cells

Background The pathogenesis of diabetic nephropathy (DN) is a complex pathophysiological process. Its precise mechanism is not fully known. In recent years it has been recognized that synthesis of various extracelluar matrix (ECM) components may increase, and that degradation of ECM may decrease in DN. It was reported heparin could inhibit mesangial cells proliferation in vitro. The main aim of this study is to explore whether heparin inhibits proliferation of mesangial cells grown in high glucose concentration and to measure the effect of heparin on matrix metalloproteinases (MMPs) expression in mesangial cells. Methods The medium contained either low glucose (5 mmol/L) or high glucose (25 mmol/L). The concentrations of heparin in the culture medium were 0, 25, 50, 100, 200 or 400 μg/mL. A metabolic (WST-1) assay was used to measure mesangial cell proliferation and Western blot analysis was used to measure MMPs expression of mesangial cells. Results Normal human mesangial cell (NHMC) proliferation was higher in high glucose (HG) medium than in low glucose (LG) medium. They showed a 1.93 fold expansion after 72 h in high glucose in contrast to a 1.63 fold expansion in low glucose. In the presence of heparin, mesangial cells proliferation was inhibited, which was more obvious at high glucose concentrations than at low glucose concentrations. In high glucose, with heparin concentration of 50, 100, 200 and 400 μg/mL, the mesangial cells showed a 0.61 fold, 0.52 fold, 0.52 fold and 0.41 fold reductions in cell number compared to cells grown without heparin. In low glucose, only concentrations of 200 μg/mL and 400 μg/mL showed reduction in cell number, namely 0.54 fold and 0.45 fold, when compared to cells grown without heparin. In Western blot analysis, MMP₁, MMP₂, MMP₃ and MMP₉ was expressed by mesangial cells expressed in both high and low glucose concentrations, which was more prominent in high glucose medium. Incubation of heparin further increased expression of MMP₁, MMP₂, MMP₃ and MMP₉. Conclusions This study suggests that glucose can accelerate mesangial cell proliferation while heparin can reduce proliferation, being more obvious at high glucose concentrations. Higher glucose concentrations led to increased MMP expression, which may take part in the regulation of mesangial matrix synthesis and degradation. Addition of heparin resulted in a corresponding increase in MMP expression, most notably at high glucose concentrations, indicating a potentially renoprotective role in DN. Foundation item: Project (30370663) supported by the National Natural Science Foundation of China     

城市污水处理过程中的有机污染物三维荧光光谱表征

采用三维荧光考察了合肥市某污水处理厂各运行节点的光谱特征,研究了城市污水处理过程中溶解性有机物的三维荧光特性变化特征。结果表明,城市生活污水中荧光类溶解性有机物主要由类蛋白有机物、紫外腐殖质和可见腐殖质构成;通过对污水生物处理构筑物各运行节点水样的荧光检测,发现在整个运行过程中特征荧光峰中心位置和强度均发生了明显的改变,说明污水中有机物的相对组成和含量随生物处理过程而变化。     

丹参水浸提液的荧光光谱特性

釆用三维和二维荧光法对中药丹参水浸提液的荧光特性进行了研究。丹参水浸提液在三维荧光等高线光谱中出现2个荧光峰,其激发波长分别为2433、37 nm,发射波长为437 nm。丹参素、丹参酚酸A、丹参酚酸B及原儿茶醛与丹参水浸提液的二维荧光光谱比较研究表明,丹参水浸提液的荧光光谱是各种丹参酚酸化合物的荧光光谱的叠加,其中丹参酚酸B对丹参水浸提液荧光强度贡献最大,且荧光发射波长相同。在近中性条件下,丹参水浸提液荧光强度与浓度之间呈良好的线性关系,可以作为定量分析丹参中总酚酸的依据。丹参水浸提液的三维荧光光谱具有指纹图谱特征,可以用于丹参的定性鉴别。在pH 7.0~12.7丹参水浸提液荧光发射光强度随pH增大呈现递减甚至消失的变化,同时丹参素的380 nm荧光有所增强,丹参酚酸化合物在碱性环境下易于水解应该是其主要原因。     

The Bisphosphonate Clodronate Modifying Hydroxyapatite Bioceramics for Bone Scaffold

1Introduction Variousbiomaterialsareincreasinglyusedasbone substitutesinorthopedicsurgery,oralandmaxillo facial surgery.Toserveasabonesubstitute,thematerialmust bebiocompatible,osteoconductive,andosteointegrative.Hydroxyapatite(HA)hasfavorablebiocompatibi…     

Sensitization and intra-molecular energy transfer of Eu<Superscript>3+</Superscript> by Tb<Superscript>3+</Superscript> in Eu-Tb binuclear complexes/PMMA

A series of Eu0.5Tb0.5(TTA)3Phen/PMMA (TTA=thenoyltrifluoroacetone,Phen=phenanthroline) and Eu0.5Tb0.5(TTA)3Dipy/PMMA (Dipy=2,2’-dipyridyl) were prepared by in-situ polymerization.The structures of the composites were characterized by IR spectra and electron spectrum.Photoluminescence properties were investigated by UV-Vis spectra and fluorescence spectra.Meanwhile,the energy transfer models were set up.The results indicated that polymer parts were attached with the rare-earth molecular parts in the composi...     

Cytocompatibility of self-assembled hydrogel from IKVAV-containing peptide amphiphile with neural stem cells

Neural Stem Cells (NSCs) were incubated with self-assembled hydrogel from IKVAV-containing peptide amphiphile (IKVAV-PA) for one week. The cytocompatibility of hydrogel was evaluated. NSCs were seeded in three-dimensional (3D) hydrogels (Experimental Group, EG) or surface of coverslips (Control Group, CG), double-labeled with Calcein-AM and PI. A growth curve of cells was obtained according to CCK-8. TEM study of hydrogel revealed a network of nanofibers. NSCs began to proliferate after 24 h of incubation, and formed bigger neurospheres at 48 h in EG than in CG. Cell proliferation activity was higher in EG than in CG (P<0.05). The self-assembled Hydrogel had good cytocompatibility and promoted the proliferation of NSCs.     

牦牛乳中主要过敏原研究

为分析牦牛乳中主要的过敏原,腹腔注射牦牛乳蛋白质免疫Balb／C小鼠．未次免疫后,小鼠摘眼球取血,测定特异性抗体和组胺质量浓度．在无菌条件下收集并培养脾淋巴细胞．Western-Blotting方法分析牦牛脱脂乳中主要过敏原组分．结果显示,牦牛脱脂乳中主要的过敏原组分为β-乳球蛋白、α-酪蛋白和β-酪蛋白,这3种过敏原蛋白质致敏强度的大小为β-乳球蛋白>α-酪蛋白>β-酪蛋白．因为脾淋巴细胞产生的细胞素IL-4、IL-5质量浓度较高而IFN-γ较低,所以牦牛乳蛋白质为Th2应答．     

Citrate-stabilized CdSe/CdS quantum dots as fluorescence probe for protein determination

A rapid, ultrasensitive and convenient fluorescence measurement technology based on the enhancement of the fluorescence intensity resulting from the interaction of functionalized CdSe/CdS quantum dots (QDs) with bovine serum albumin (BSA) was proposed. The citrate-stabilized CdSe/CdS (QDs) were synthesized by using Se powder and Na₂S as precursors instead of any pyrophoric organometallic precursors. The modified CdSe/CdS QDs are brighter and more stable against photobleaching in comparison with organic fluorophores. At pH 7.0, the fluorescence signal of CdSe/CdS is enhanced by increasing the concentration of BSA in the range of 0.1–10 μg/mL, and the low detection limit is 0.06 μg/mL. A linear relationship between the enhanced fluorescence peak intensity (ΔF) and BSA concentration (c) is established using equation ΔF=50.7c+16.4 (R=0.996 36). Results of determination for BSA in three synthetic samples are identical with the true values, and the recovery (98.9%–102.4%) and relative standard deviation (RSD, 1.8%–2.5%) are satisfactory.     

Fluorescence detection for H<Subscript>2</Subscript>PO<Subscript>4</Subscript><Superscript>-</Superscript> based on carbon dots/Fe<Superscript>3+</Superscript> composite

A novel fluorescent probe for H₂PO₄ ^- was designed and fabricated based on the carbon dots/Fe³⁺ composite. The carbon dots were synthesized by an established one-pot hydrothermal method and characterized by transmission electron microscope, X-ray diffractometer, UV-Vis absorption spectrometer and fluorescence spectrophotometer. The carbon dots/Fe³⁺ composite was obtained by aqueous mixing of carbon dots and FeCl₃, and its fluorescence property was characterized by fluorescence spectrophotometer. The fluorescence of carbon dots was quenched by aqueous Fe³⁺ cations, resulting in the low fluorescence intensity of the carbon dots/Fe³⁺ composite. On the other hand, H₂PO₄ ^- reduced the concentration of Fe³⁺ by chemical reaction and enhanced the fluorescence of the carbon dots/Fe³⁺ composite. The Stern-Volmer equation was introduced to describe the relation between the relative fluorescence intensity of the carbon dots/Fe³⁺ composite and the concentration of H₂PO₄ ^-, and a fine linearity (R ²=0.997) was found in the range of H₂PO₄ ^- concentration of 0.4-12 mM.     

植物甾醇-共轭亚油酸酯对结肠癌细胞增殖的抑制作用

观察植物甾醇-共轭亚油酸酯对结肠癌HT-29细胞增殖的作用,探讨其可能的作用机制。采用体外培养结肠癌HT-29细胞的方法,施加不同浓度植物甾醇-共轭亚油酸酯(100、150、200、250、300μmol/L),观察对细胞增殖的影响。应用四唑盐比色法(MTT法)观察细胞增殖抑制情况,倒置荧光显微镜观察细胞形态学特征。结果显示,植物甾醇-共轭亚油酸酯各组较对照组增殖降低,且存在剂量-效应关系(100～300μmol/L)和时间-效应关系(24、48、72h),说明植物甾醇-共轭亚油酸酯抑制结肠癌HT-29细胞增殖。     

Gap-plasmon of Fe<Subscript>3</Subscript>O<Subscript>4</Subscript>@Ag core-shell nanostructures for highly enhanced fluorescence detection of Rhodamine B

A novel gap-plasmon of Fe₃O₄@Ag core-shell nanoparticles for surface enhanced fluorescence detection of Rhodamine B (RB) was developed. Fe₃O₄@Ag core-shell nanostructures with Ag shell and Fe₃O₄ core were synthetized by self-assembled method with the assistance of 3-mercaptopropyl trimethoxy silane (MPTS). To study the RB fluorescence enhanced by gap-plasmon, the fluorescence properties of RB on the substrates with different nanogap densities were systematically investigated, and the results showed that the fluorescence intensity of RB on Fe₃O₄@Ag core-shell NPs substrate was much stronger than that on bare glass substrate, and the fluorescence intensity was further improved by using multilayer Fe₃O₄@Ag core-shell NPs substrate which had higher nanogap density. Different from the mechanism that is based on the maximum overlap of the surface plasmon resonance (SPR) band and emission band, the mechanism of the fluorescence enhancement in our work is based on the localized surface plasmon (LSP) and the gap plasmon near-field coupling with the Fe₃O₄@Ag core-shell NPs. Besides, the detection limit obtained was as low as 1×10^-7 mol/L, and the Fe₃O₄@Ag core-shell NPs substrate had high selectivity for RB fluorophores. It was demonstrated that the Fe₃O₄@Ag core-shell NPs substrate had activity, good stability, and selectivity for fluorescence detection of RB. And the detection of RB by the surface plasmon enhanced fluorescence was more convenient and rapid than the traditional detection methods in previous works.     

Luminescent properties of BaO-La2O3-B2O3 glasses with dopant

The luminescent properties of glasses synthesized in air atmosphere by conventional high temperature process were stud{ed. The emissions spectra of Eu^2 and Eu^3 were observed in BaO-La2O3-B2O3-Eu2O3 glasses.The results show that the broad emission peaks at 430 nm correspond to 5d→4f emission transition of Eu^2 , the sharp emission peaks at 592, 616, 650 and 250 nm correspond to 5^D0→1Fj(j=1--4) emission transition of Eu^3 ,respectively, which indicates that the BaO-La2O3a-B2O3-Eu2O3 glass can convert ultraviolet and green omponents of sunlight into blue and red light so as to increase the intensity of blue and red light, respectively. The luminescent in--tensity of Eu^2 increases with increasing the molar ratio of Tb^3 in BaO-La2O3-B2O3-Eu2O3a-Tb4O3 glasses, whereas the luminescent intensity of Eua^3 decreases. So the luminescent intensity of Eu(Ⅲ,Ⅱ) is influenced by Tb^3 .These phenomena can be explained by electron transfer mechanism; Eu^3 (4f6) Tb^3 (4f^8)→Eu^2 (4f′) Tb^4 (4f′). Taking advantage of the luminescent properties of BaO-La2O3-B2O3-Eu2O3 glasses, light-conversion glass for agriculture can be produced.