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1.
Water pollution by microorganisms of fecal origin is a current world-wide public health concern. Total coliforms, fecal coliforms (Escherichia coli) and enterococci are indicators commonly used to assess the microbiological safety of water resources. In this study, influent water samples and treated water were collected seasonally from a water treatment plant and two major water wells in a Black Belt county of Alabama and evaluated for water quality indicator bacteria. Influent river water samples serving the treatment plant were positive for total coliforms, fecal coliforms (E. coli), and enterococci. The highest number of total coliform most probable number (MPN) was observed in the winter (847.5 MPN/100 mL) and the lowest number in the summer (385.6 MPN/100 mL). Similarly E. coli MPN was substantially higher in the winter (62.25 MPN/100 mL). Seasonal variation of E. coli MPN in influent river water samples was strongly correlated with color (R2 = 0.998) and turbidity (R2 = 0.992). Neither E. coli nor other coliform type bacteria were detected in effluent potable water from the treatment plant. The MPN of enterococci was the highest in the fall and the lowest in the winter. Approximately 99.7 and 51.5 enterococci MPN/100 mL were recorded in fall and winter seasons respectively. One-way ANOVA tests revealed significant differences in seasonal variation of total coliforms (P < 0.05), fecal coliforms (P < 0.01) and enterococci (P < 0.01). Treated effluent river water samples and well water samples revealed no enterococci contamination. Representative coliform bacteria selected by differential screening on Coliscan Easygel were identified by 16S ribosomal RNA gene sequence analysis. E. coli isolates were sensitive to gentamicin, trimethoprim/sulfamethazole, ciprofloxacin, vancomycin, tetracycline, ampicillin, cefixime, and nitrofurantoin. Nonetheless, isolate BO-54 displayed decreased sensitivity compared to other E. coli isolates. Antibiotic sensitivity pattern can be employed in microbial source tracking. 相似文献
2.
Thomas R Deetz Eric M Smith Sagar M Goyal Charles P Gerba John J Vollet III Liane Tsai Herbert L DuPont Bruce H Keswick 《Water research》1984,18(5):567-571
Rotaviruses have been implicated as a major cause of childhood and traveler's diarrhea in developing countries. Since water is known to be a vehicle of transmission of other enteric viruses, we sought to determine if water could play a role in the transmission of rotavirus infections in a developing nation by applying recently developed techniques for the concentration of viruses from tapwater and environmental (lake, river, ocean and aqueduct) water in Mexico. In an initial survey during the rainy season in August 1978, rotavirus was detected in 10 of 10 drinking water samples and coxsackie B4 or B6 virus in 5 of 10. In a larger survey during the dry season in December 1979, rotavirus was recovered from 3 and enteric viruses from 8 of 21 drinking water samples. Water quality data, available for the 1979 survey, indicated that while many tapwater samples did not meet U.S. coliform standards, some samples containing infectious virus did. Our data suggest that current bacteriological water quality standards for potable water do not reflect viral contamination. 相似文献
3.
Within developing countries, a multitude of problems that affect the water supply process can result in the contamination of water taps. While machine learning applications have become popular for attaining efficient water quality predictions, acquiring the necessary data for modelling for developing countries is challenging. This study constructs water quality prediction models by machine learning with a pseudo-pipeline network to complement the missing data of the water supply process. Using both water source and water tap quality information measured by the Government of Nepal, we apply the three machine learning models: support vector machine (SVM), random forest (RF) and LightGBM. Furthermore, we also apply a traditional statistical method—logistic regression (LR)—to the prediction of the Escherichia coli (E. coli) contamination in water taps. With some input variables (such as the length from the nearest sources) obtained from the pseudo-pipeline network, the results show that SVM has stable and high accuracy for both the 26 cities (70%) and for the 25 cities except for Kathmandu (79%). LR performed a significantly lower accuracy for all cities (61%) than for 25 cities (79%). Additionally, we show that our method can be applied to other regions where a water quality survey has not yet been conducted. 相似文献
4.
Rainwater catchment systems (RWCS) in Micronesia were sampled to assess their bacteriological water quality and to determine which RWCS characteristics had a significant impact on water quality. Total and fecal coliform bacteria tests were used to evaluate 203 catchments on 10 islands. Fifty-seven percent of the RWCS had no fecal coliform bacteria and 61% had fewer than 10 total coliform bacteria per 100 ml. Catchment characteristics were found to have a statistically significant effect on total coliform bacteria levels but they did not affect faecal coliform bacteria concentrations. Rainwater catchment systems were found to provide acceptable water in most cases but disinfection prior to consumption is still highly recommended. 相似文献
5.
G. Wilkes J. Brassard T.A. Edge V. Gannon C.C. Jokinen T.H. Jones N. Neumann K.D.M. Pintar N. Ruecker P.J. Schmidt M. Sunohara E. Topp D.R. Lapen 《Water research》2013
Over 3500 individual water samples, for 131 sampling times, targeting waterborne pathogens/fecal indicator bacteria were collected during a 7-year period from 4 sites along an intermittent stream running through a small livestock pasture system with and without cattle access-to-stream restriction measures. The study assessed the impact of cattle pasturing/riparian zone protection on: pathogen (bacterial, viral, parasite) occurrence, concentrations of fecal indicators, and quantitative microbial risk assessments (QMRA) of the risk of Cryptosporidium, Giardia and Escherichia coli O157:H7 infection in humans. Methodologies were developed to compute QMRA mean risks on the basis of water samples exhibiting potentially human infectious Cryptosporidium and E. coli based on genotyping Crytosporidium, and E. coli O157:H7 presence/absence information paired with enumerated E. coli. All Giardia spp. were considered infectious. No significant pasturing treatment effects were observed among pathogens, with the exception of Campylobacter spp. and E. coli O157:H7. Campylobacter spp. prevalence significantly decreased downstream through pasture treatments and E. coli O157:H7 was observed in a few instances in the middle of the unrestricted pasture. Densities of total coliform, fecal coliform, and E. coli reduced significantly downstream in the restricted pasture system, but not in the unrestricted system. Seasonal and flow conditions were associated with greater indicator bacteria densities, especially in the summer. Norovirus GII was detected at rates of 7–22% of samples for all monitoring sites, and rotavirus in 0–7% of samples for all monitoring sites; pasture treatment trends were not evident, however. Seasonal and stream flow variables (and their interactions) were relatively more important than pasture treatments for initially stratifying pathogen occurrence and higher fecal indicator bacteria densities. Significant positive associations among fecal indicator bacteria and Campylobacter spp. detection were observed. For QMRA, adjusting for the proportion of Cryptosporidium spp. detected that are infectious for humans reduces downstream risk estimates by roughly one order of magnitude. Using QMRA in this manner provides a more refined estimate of beneficial management practice effects on pathogen exposure risks to humans. 相似文献
6.
7.
In-pipe water quality monitoring in water supply systems under steady and unsteady state flow conditions: a quantitative assessment 总被引:1,自引:0,他引:1
Monitoring the quality of drinking water from the treatment plant to the consumers tap is critical to ensure compliance with national standards and/or WHO guideline levels. There are a number of processes and factors affecting the water quality during transmission and distribution which are little understood. A significant obstacle for gaining a detailed knowledge of various physical and chemical processes and the effect of the hydraulic conditions on the water quality deterioration within water supply systems is the lack of reliable and low-cost (both capital and O & M) water quality sensors for continuous monitoring. This paper has two objectives. The first one is to present a detailed evaluation of the performance of a novel in-pipe multi-parameter sensor probe for reagent- and membrane-free continuous water quality monitoring in water supply systems. The second objective is to describe the results from experimental research which was conducted to acquire continuous water quality and high-frequency hydraulic data for the quantitative assessment of the water quality changes occurring under steady and unsteady-state flow conditions. The laboratory and field evaluation of the multi-parameter sensor probe showed that the sensors have a rapid dynamic response, average repeatability and unreliable accuracy. The uncertainties in the sensor data present significant challenges for the analysis and interpretation of the acquired data and their use for water quality modelling, decision support and control in operational systems. Notwithstanding these uncertainties, the unique data sets acquired from transmission and distribution systems demonstrated the deleterious effect of unsteady state flow conditions on various water quality parameters. These studies demonstrate: (i) the significant impact of the unsteady-state hydraulic conditions on the disinfectant residual, turbidity and colour caused by the re-suspension of sediments, scouring of biofilms and tubercles from the pipe and increased mixing, and the need for further experimental research to investigate these interactions; (ii) important advances in sensor technologies which provide unique opportunities to study both the dynamic hydraulic conditions and water quality changes in operational systems. The research in these two areas is critical to better understand and manage the water quality deterioration in ageing water transmission and distribution systems. 相似文献
8.
《Urban Water Journal》2013,10(3):240-251
Centralised water distribution systems in developing countries currently are operated intermittently to a very large extent. As a consequence of the numerous negative impacts associated with intermittent water distribution, functioning water supplies in the long run absolutely require the introduction of a continuous mode of water distribution. This article presents a conceptual approach to the successive introduction of a continuous water distribution. The approach is based on existing systems originally designed for continuous distribution but currently run in the intermittent mode. The focus is on the difficulties in planning and implementing the complex processes of database organisation, restructuring and changing the mode of operation, which are harmonised in a comprehensive process. The approach is explained for the case of the water distribution system in Béni Abbès, Algeria. 相似文献
9.
Monitoring marine recreational water quality using multiple microbial indicators in an urban tropical environment 总被引:1,自引:0,他引:1
The microbial water quality at two beaches, Hobie Beach and Crandon Beach, in Miami-Dade County, Florida, USA was measured using multiple microbial indicators for the purpose of evaluating correlations between microbes and for identifying possible sources of contamination. The indicator microbes chosen for this study (enterococci, Escherichia coli, fecal coliform, total coliform and C. perfringens) were evaluated through three different sampling efforts. These efforts included daily measurements at four locations during a wet season month and a dry season month, spatially intensive water sampling during low- and high-tide periods, and a sand sampling effort. Results indicated that concentrations did not vary in a consistent fashion between one indicator microbe and another. Daily water quality frequently exceeded guideline levels at Hobie Beach for all indicator microbes except for fecal coliform, which never exceeded the guideline. Except for total coliform, the concentrations of microbes did not change significantly between seasons in spite of the fact that the physical-chemical parameters (rainfall, temperature, pH, and salinity) changed significantly between the two monitoring periods. Spatially intense water sampling showed that the concentrations of microbes were significantly different with distance from the shoreline. The highest concentrations were observed at shoreline points and decreased at offshore points. Furthermore, the highest concentrations of indicator microbe concentrations were observed at high tide, when the wash zone area of the beach was submerged. Beach sands within the wash zone tested positive for all indicator microbes, thereby suggesting that this zone may serve as the source of indicator microbes. Ultimate sources of indicator microbes to this zone may include humans, animals, and possibly the survival and regrowth of indicator microbes due to the unique environmental conditions found within this zone. Overall, the results of this study indicated that the concentrations of indicator microbes do not necessarily correlate with one another. Exceedence of water quality guidelines, and thus the frequency of beach advisories, depends upon which indicator microbe is chosen. 相似文献
10.
Water lice, Asellus aquaticus (isopoda), frequently occur in drinking water distribution systems where they are a nuisance to consumers and water utilities. Whether they are solely an aesthetic problem or also affect the microbial water quality is a matter of interest. We studied the influence of A. aquaticus on microbial water quality in non-chlorinated drinking water in controlled laboratory experiments. Pure cultures of the indicator organisms Escherichia coli and Klebsiella pneumoniae and the pathogen Campylobacter jejuni as well as naturally occurring heterotrophic drinking water bacteria (measured as heterotrophic plate counts, HPC) were investigated in microcosms at 7 °C, containing non-sterilised drinking water, drinking water sediment and A. aquaticus collected from a non-chlorinated ground water based drinking water supply system. Concentrations of E. coli, K. pneumoniae and C. jejuni decreased over time, following a first order decay with half lives of 5.3, 18.4 and 1.3 days, respectively. A. aquaticus did not affect survival of indicators and pathogens substantially whereas HPC were influenced by presence of dead A. aquaticus. Growth rates increased with an average of 48% for bacteria grown on R-2A agar and an average of 83% for bacteria grown on yeast extract agar when dead A. aquaticus were present compared to no and living A. aquaticus present. A. aquaticus associated E. coli, K. pneumoniae and C. jejuni were measured (up to 25 per living and 500 per dead A. aquaticus) and so were A. aquaticus associated heterotrophic bacteria (>1.8*104 CFU per living and >6*104 CFU per dead A. aquaticus). A. aquaticus did not serve as an optimised habitat that increased survival of indicators and pathogens, since A. aquaticus associated E. coli, K. pneumoniae and C. jejuni were only measured as long as the bacteria were also present in the water and sediment. 相似文献
11.
A passive surveillance system captured information on 34,904 microbiological samples from 11,233 private drinking water supplies within England as well as the associated constructional, climatic and environmental variables. Escherichia coli was detected in 6588 (18.87%) of samples and at least one positive sample was detected from 3638 (32.39%) of sites. However, this estimate of supplies failing to meet the European drinking water E. coli standard was probably an underestimate as the more samples taken per supply, the more likely the supply was to fail. A multivariable model of private water supplies data showed a strong seasonal impact, with samples between January and May being significantly less contaminated with E. coli than samples between June and December. Samples from springs (OR 2.5, CI 2.0-3.1) or surface waters (OR 2.4, CI 0.8-7.0) were more likely to fail than groundwater sources, as were supplies with no effective treatment (OR1.8, CI 1.5-2.3). Commercial supplies were less likely to fail than domestic supplies (OR 0.63, CI 0.48-0.83) and the probability of failure was linearly associated with the density of sheep in the area and rainfall on the previous day. A Monte Carlo modelling approach was used to estimate that, had sufficient samples been taken, 54% (95% confidence intervals 49-59%) of all private water supplies in England were likely to be unsatisfactory. These findings will be able to inform risk assessments of private water supplies prior to microbiological results being available. 相似文献
12.
Intermittent water supply systems are characterised by schedules that subject water consumers to less than 24 hours a day or less than 7 days of water supply a week. Causes and problems of water supply intermittency and their management options are generally discussed in isolation. This obscures challenges involved in minimising intermittency problems. Through reviewing of the literature relevant to intermittency causal factors, problems and their solution options, this paper finds that the interplay between political, social, economic, natural and technical factors, as they contribute to the development and sustenance of water supply intermittency, calls for interdisciplinary approaches to resolving the problems. These approaches will facilitate understanding of the challenges and development of integrated sustainable interventions. Where water resources are abundant, these interventions should involve conversion from intermittent to continuous supply as the ultimate option to handling supply intermittency problems. For this, development of systematic conversion procedures is recommended. 相似文献
13.
A study was conducted to detect the source of fecal indicator bacteria and measure the resulting impairment of water quality of the Sturgeon River at Sturgeon Falls, Ontario, Canada. The bacteriological water quality of the river above the dam at Sturgeon Falls was fairly good, while below the dam the water quality was degraded. The principal input of fecal indicator bacteria was traced to the paper mill at Sturgeon Falls. The impairment of water quality was detected all the way to recreational areas near the mouth of the river. The impaired stretch of the river had diminished aesthetic appeal. Other sources of indicator bacteria could be masked by the large numbers of similar bacteria discharged from the paper mill. The principal fecal coliform was Klebsiella pneumoniae. In pulp mill wastes the fecal origin of this bacterium can be disputed, and so its presence in large numbers interfered with the interpretation of fecal coliform results. K. pneumoniae is also an opportunistic pathogen which causes infections in humans. Thermotolerant oxidase positive bacteria which were isolated from the paper mill wastes, register as false positives in the fecal coliform test causing problems in interpretation. Pseudomonas aeruginosa was detected in the pulp mill wastewater, and at a level of 82 PA 100 ml−1 in recreational areas of the river. This was considered to be of some hazard to users of the water. In addition, Escherichia coli was detected in the mill wastewater, at about 200 EC 100 ml−1, and this probably indicated some measure of fecal pollution though likely of animal origin. Finally the nutrient rich wastewater led to an approx. 50-fold increase in density of aerobic heterotrophic bacteria in the river water, as well as the production of a slimy filamentous growth on surfaces, stones and wooden pilings, in the river. The principal organism in this slime was the fungus Leptomitus. 相似文献
14.
Recent epidemiology studies examining U.S. recreational water exposure and illness relationships have focused primarily on coastal and Great Lakes beaches. Human-made lakes in the U.S. have received little attention in epidemiology studies despite contributing to more waterborne disease epidemics annually than coastal U.S. waters. In a comprehensive beach cohort study, we examined relationships between water quality indicators and reported adverse health outcomes among users of a beach at an inland U.S. lake. Human health data was collected over 26 swimming days during the 2009 swimming season in conjunction with water quality measurements. Adverse health outcomes were reported 8-9 days post-exposure via a phone survey. Wading, playing or swimming in the water was observed to be a significant risk factor for GI illness (adjusted odds ratio (AOR) of 3.2; CI 1.1, 9.0). Among water users, Escherichia coli density was significantly associated with elevated GI illness risk where the highest E. coli quartile was associated with an AOR of 7.0 (CI 1.5, 32). GI illness associations are consistent with previous freshwater epidemiology studies. Our findings are unique in that our observations of positive associations with GI illness risk are based upon a single daily E. coli measurement. Lastly, this study focused on an understudied issue, illness risk at inland reservoirs. Our results support the usefulness of E. coli as a health-relevant indicator of water quality for this inland U.S. beach. 相似文献
15.
Kate Ellis Claire Gowdy Natalie Jakomis Bernadette Ryan Claire Thom Catherine A. Biggs 《Urban Water Journal》2018,15(2):101-108
Bacteriological failure investigations are crucial in the provision of safe, clean drinking water as part of a process of quality assurance and continual improvement. However, the financial implications of investigating coliform and Escherichia coli failures during routine water quality monitoring are poorly understood in the industry. The investigations for 737 coliform and E. coli failures across five UK water companies were analysed in this paper. The principal components of investigation costs were staff hours worked, re-samples collected, transportation, and special investigatory activities related to the sample collection location. The average investigation costs ranged from £575 for a customer tap failure to £4,775 for a water treatment works finished water failure. These costs were compared to predictions for US utilities under the Revised Total Coliform Rule. Improved understanding of the financial and staffing implications of investigating bacteriological failures can be used to budget operational expenditures and justify increased funding for preventive strategies. 相似文献
16.
Water service interruptions often impose considerable costs on water users, and lead more households to invest in in-home water storage infrastructure. Studying the value households assign to continuous water supply may help design more effective policies. Using a unique primary dataset from Guatemala City (n = 567), we estimate hedonic models of rental prices to derive marginal values of fewer water service interruptions and in-home water storage infrastructure. Results indicate that, on average, rental prices decrease by 0.7% with each day of water service interruptions and increase by 10% for housing units with storage infrastructure relative to those without it. 相似文献
17.
The loading of microbial contaminants was examined within the Thomas Brook watershed, a 784 ha mixed land-use catchment located in the headwaters of the Cornwallis River drainage basin (Nova Scotia, Canada). The objectives were to: (i) examine spatial and temporal characteristics of fecal bacteria loading during the growing season from five subwatersheds, and (ii) develop areal fecal indicator organism export coefficients for rural landscapes. Fecal coliform, Escherichia coli, total suspended solids (TSS) concentrations and stream flow were monitored at five locations in the watershed over six consecutive growing seasons (May-Oct, 2001-2006). A nested watershed monitoring approach was used to determine bacterial loading from distinct source types (residential vs. agricultural) during both baseflow and stormflow periods. Areal bacterial loading rates increased in each nested watershed moving downstream through the watershed and were highest in the three subcatchments dominated by agricultural activities. Upper watershed bacterial loading throughout the growing season from an agricultural subcatchment (Growing Season Avg 8.92 × 1010 CFU ha−1) was consistently higher than a residential subcatchment (Growing Season Avg 8.43 × 109 CFU ha−1). As expected, annual average stormflow bacterial loads were higher than baseflow loads, however baseflow loads still comprised between 14 and 35% of the growing season bacterial loads in the five subwatersheds. Fecal bacteria loads were greater during years with higher annual precipitation. A positive linear relationship was observed between E. coli and TSS loading during the 2005 and 2006 growing seasons when both parameters were monitored, indicating that the processes of sediment transport and bacterial transport are linked. It is anticipated that computed areal microbial loading coefficients will be useful in developing watershed management plans. More intensive sampling during stormflow events is recommended for improving these coefficients. 相似文献
18.
Microbial quality assessment of household greywater 总被引:1,自引:0,他引:1
O'Toole J Sinclair M Malawaraarachchi M Hamilton A Barker SF Leder K 《Water research》2012,46(13):4301-4313
A monitoring program was undertaken to assess the microbial quality of greywater collected from 93 typical households in Melbourne, Australia. A total of 185 samples, comprising 75 washing machine wash, 74 washing machine rinse and 36 bathroom samples were analysed for the faecal indicator Escherichia coli. Of these, 104 were also analysed for genetic markers of pathogenic E coli and 111 for norovirus (genogroups GI and GII), enterovirus and rotavirus using RT-PCR. Enteric viruses were detected in 20 out of the 111 (18%) samples comprising 16 washing machine wash water and 4 bathroom samples. Eight (7%) samples were positive for enterovirus, twelve (11%) for norovirus genogroup GI, one (1%) for norovirus genogroup GII and another (1%) for rotavirus. Two washing machine samples contained more than one virus. Typical pathogenic E. coli were detected in 3 out of 104 (3%) samples and atypical enteropathogenic E. coli in 11 (11%) of samples. Levels of indicator E. coli were highly variable and the presence of E. coli was not associated with the presence of human enteric viruses in greywater. There was also little correlation between reported gastrointestinal illness in households and detection of pathogens in greywater. 相似文献
19.
A dearth in infrastructure and operations significantly reduces the expected benefits of safe drinking water provision. Intermittent water supplies are characterized by inefficient demand and supply management owing to operational inadequacies eventually causing physical deterioration of infrastructure and inconvenience to consumers, resulting in consumer dissatisfaction. Conversion from intermittent to continuous water supply was undertaken in a demonstration zone of Nagpur, India, with a population of about 150,000 people. Data related to the infrastructural, operational, managerial and financial capabilities were used to determine service level benchmarks (SLBs) — pre- and post-intervention — to quantify the improvement due to continuous water supply interventions. The post-intervention data analysis clearly indicated substantial improvement in post-intervention SLBs, consequently paving the way for the conversion of the water supply of the entire city to continuous mode. 相似文献
20.
Earlier results indicated that, for an average household, self-sufficiency in water supply can be achieved by following the Urban harvest Approach (UHA), in a combination of demand minimization, cascading and multi-sourcing. To achieve these results, it was assumed that all available local resources can be harvested. In reality, however, temporal, spatial and location-bound factors pose limitations to this harvest and, thus, to self-sufficiency. This article investigates potential spatial and temporal limitations to harvest local water resources at building level for the Netherlands, with a focus on indoor demand. Two building types were studied, a free standing house (one four-people household) and a mid-rise apartment flat (28 two-person households). To be able to model yearly water balances, daily patterns considering household occupancy and presence of water using appliances were defined per building type. Three strategies were defined. The strategies include demand minimization, light grey water (LGW) recycling, and rainwater harvesting (multi-sourcing). Recycling and multi-sourcing cater for toilet flushing and laundry machine. Results showed that water saving devices may reduce 30% of the conventional demand. Recycling of LGW can supply 100% of second quality water (DQ2) which represents 36% of the conventional demand or up to 20% of the minimized demand. Rainwater harvesting may supply approximately 80% of the minimized demand in case of the apartment flat and 60% in case of the free standing house. To harvest these potentials, different system specifications, related to the household type, are required. Two constraints to recycle and multi-source were identified, namely i) limitations in the grey water production and available rainfall; and ii) the potential to harvest water as determined by the temporal pattern in water availability, water use, and storage and treatment capacities. 相似文献