京尼平苷的药代动力学研究进展

环烯醚萜类化合物京尼平苷具有降血糖、抗炎、神经保护、对大鼠心肌缺血再灌注损伤的保护等多种药理作用,具有广泛的药用价值。目前,对京尼平苷的药代动力学研究已取得一些成果,本文系统阐述了京尼平苷单体、栀子复方药物中及疾病状态下京尼平苷药代动力学方面的研究,为京尼平苷的进一步研究和开发应用提供参考。     

碱性溶液萃取大黄蒽醌类产品动力学的研究

以大黄酸为代表,利用恒界面池法对碱性溶液萃取分离大黄蒽醌类产品的动力学进行了初步研究,以确定大黄中蒽醌类物质的萃取类型。研究结果表明,KHCO3水溶液萃取大黄酸的萃取动力学模式为化学反应控制模型。     

氨茶碱在大鼠体内的药代动力学研究

用高效液相色谱法研究氨茶碱在大鼠体内的药代动力学.色谱柱为Hypersil BDS C18 (4.6 mm×200mm,5 μm),流动相为甲醇—水(24 ∶ 76),检测波长为274 nm.结果表明,大鼠灌胃给予氨茶碱后,其代谢符合二室模型;由统计矩参数表示的主要药代动力学参数如下:tmax=(1.083士0.195...     

超临界二氧化碳流体萃取大黄蒽醌动力学模型

通过对萃取固定床层进行质量衡算建立了超临界CO₂流体萃取动力学模型.然后采用BP神经网络拟合实验条件下的萃取动力学曲线,并将之与动力学模型试差法求解所得的萃出曲线进行对照确定模型参数k_La.结果表明,BP神经网络能很好地模拟大黄蒽醌的萃取动力学曲线,网络训练误差和预测误差分别为1.5%和3.5%.确定参数后的动力学模型可用于对萃取床层作较为精确的定量描述：模拟所得萃取穿透曲线与大黄蒽醌萃取实验结果相比AARD误差在10%左右.与前人模型相比,该模型具有精度好,获取参数时的实验条件相对宽松等优点.     

大黄中总蒽醌含量提取工艺研究

目的研究中药大黄总蒽醌的最佳醇提工艺。本文采用乙醇回流提取法,以大黄中总蒽醌含量为考察指标,通过温度、浓度、固液比梯度实验,采用单因素考察得出较适提取大黄中总蒽醌成分的工艺条件为:大黄中总蒽醌含量的提取工艺为:乙醇浓度70%,提取温度70℃,提取时间1 h,固液比1∶20。     

干扰素脂质体的药代动力学和生物利用度

目的研究干扰素α2b脂质体药代动力学和生物利用度。方法采用旋转逆相蒸发法制备干扰素α2b脂质体。将小鼠分成2组,分别尾静脉注射干扰素α2b脂质体(L-IFN)和干扰素α2b(IFN),给药后分8个时间点采血,并取肝脏组织检测干扰素α2b含量。计算药代动力学参数和生物利用度。结果L-IFN组小鼠血液及肝脏组织中干扰素α2b消除速度低于IFN组,血中相对生物利用度为237·47%,肝脏中相对生物利用度为191·73%。结论干扰素α2b脂质体可明显提高小鼠的吸收和利用。     

聚乙二醇化促红细胞生成素在猕猴体内的药代动力学分析

目的分析聚乙二醇(相对分子质量40000)化促红细胞生成素(PEG-EPO)在猕猴体内的药代动力学特征。方法建立检测PEG-EPO的双抗体夹心ELISA方法,并进行验证。用1、3和9mg/kg3个剂量的PEG-EPO经静脉注射猕猴,给药后各时间点检测猕猴体内的血药浓度,用DAS软件获取各剂量组药代动力学参数。结果PEG-EPO在猕猴体内的代谢过程符合二室开放模型,1、3和9mg/kg3个剂量的PEG-EPO的消除半衰期(t1/2β)分别为(51.89300±5.39558)、(42.48550±3.01606)和(56.46130±6.81932)h;AUC0～144分别为(109.32±9.82)、(236.23±36.16)和(722.55±84.31)mg/L·h。结论相对分子质量为40000的PEG修饰的EPO能显著延长其在猕猴体内的半衰期。     

HPLC-MS/MS法测定美金刚体内浓度及药代动力学研究

建立高效液相色谱-串联质谱(HPLC-MS/MS)方法测定大鼠血浆中美金刚,并用于美金刚双羟萘酸混悬剂的体内药动学研究.大鼠肌肉注射美金刚双羟萘酸混悬剂,在预设的时间点采集血浆样本,经沉淀蛋白后进行UPLC-MS/MS分析.用0.1％甲酸水溶液和甲醇梯度洗脱,Shim-pack GIST C18色谱柱分离,电喷雾离子源...     

高效液相色谱法测定胆清胶囊中大黄素含量的研究

采用高效液相色谱法，对制剂中大黄所含大黄素的含量测定进行了方法研究。实验结果表明，本法快速、简便、回收率高，结果准确可靠。     

Organ Specific Differences in Alteration of Aquaporin Expression in Rats Treated with Sennoside A,Senna Anthraquinones and Rhubarb Anthraquinones

Senna and rhubarb are often used as routine laxatives, but there are differences in mechanism of action and potential side effects. Here, we studied metabolites of senna anthraquinones (SAQ), rhubarb anthraquinones (RAQ) and their chemical marker, sennoside A (SA), in a rat diarrhea model. In in vitro biotransformation experiments, SAQ, RAQ and SA were incubated with rat fecal flora solution and the metabolites produced were analyzed using HPLC. In in vivo studies, the same compounds were investigated for purgation induction, with measurement of histopathology and Aqps gene expression in six organs. The results indicated that SAQ and RAQ had similar principal constituents but could be degraded into different metabolites. A similar profile of Aqps down-regulation for all compounds was seen in the colon, suggesting a similar mechanism of action for purgation. However, in the kidneys and livers of the diarrhea-rats, down-regulation of Aqps was found in the RAQ-rats whereas up-regulation of Aqps was seen in the SAQ-rats. Furthermore, the RAQ-rats showed lower Aqp2 protein expression in the kidneys, whilst the SA-rats and SAQ-rats had higher Aqp2 protein expression in the kidneys. This may have implications for side effects of SAQ or RAQ in patients with chronic kidney or liver diseases.     

马氏珠母贝壳对大黄水提液吸附效果的研究

基于光学吸收和散射原理,判断不同影响因素对吸附效果的影响。马氏珠母贝壳对大黄水提液吸附的最佳工艺条件：吸附温度30℃,吸附时间30min,贝壳用量为1g。马氏珠母贝壳对大黄水提液有较强的吸附效果。提高了大黄水提液的澄清度和药品质量,优化生产工艺。     

乌药化学成分研究进展

综述了近年来对我国传统中药乌药中的化学成分的研究进展.重点讨论了乌药根、茎、叶中倍半萜、生物碱、黄酮类、单宁类、甾体的化学成分。     

The Health Benefits of Emodin,a Natural Anthraquinone Derived from Rhubarb—A Summary Update

Emodin (6-methyl-1,3,8-trihydroxyanthraquinone) is a naturally occurring anthraquinone derivative found in roots and leaves of various plants, fungi and lichens. For a long time it has been used in traditional Chinese medicine as an active ingredient in herbs. Among other sources, it is isolated from the rhubarb Rheum palmatum or tuber fleece-flower Polygonam multiflorum. Emodin has a wide range of biological activities, including diuretic, antibacterial, antiulcer, anti-inflammatory, anticancer and antinociceptive. According to the most recent studies, emodin acts as an antimalarial and antiallergic agent, and can also reverse resistance to chemotherapy. In the present work the potential therapeutic role of emodin in treatment of inflammatory diseases, cancers and microbial infections is analysed.     

何首乌结合型蒽醌中大黄素成分提取工艺的研究

目的比较不同提取工艺对何首乌中大黄素含量的影响。方法采用HPLC法对提取物中大黄素进行定量分析,比较提取溶液浓度、温度和加酸处理对两种提取方法的影响,确定提取何首乌中结合型蒽醌中大黄素的最佳工艺条件。结果以75%乙醇为提取溶媒采用回流提取方法,提取90 min,在干燥后的提取物中加20%盐酸酸化提取,在此提取工艺下,提取物中大黄素的含量可达0.0730%。结论不同提取工艺提取何首乌中大黄素含量差异较大,此结果为进一步开发利用何首乌资源提供科学的理论依据。     

虎杖中抗氧化成分的提取分离及其活性研究

提取了虎杖中具有抗氧化活性的总蒽醌(TA),并从总蒽醌中依次分离出强酸成分(SAP)、中酸成分(MAP)和弱酸成分(WAP)。然后采用二苯代苦味酰自由基(DPPH)法对不同质量浓度的TA、SAP、MAP和WAP进行了抗氧化活性测定。TA、SAP、MAP和WAP质量浓度分别为0.2 g/L、0.5 g/L、0.8 g/L、1.2 g/L时,对自由基的最大清除率依次分别为:29.5%、67.1%、85.7%、87.5%;34.0%、74.2%、86.5%、95.4%;9.8%、24.0%、35.2%、47.3%;6.5%、11.1%、19.6%、19.9%。TA、SAP、MAP和WAP对自由基均有程度不同的清除作用,其中SAP的效果最佳,当其质量浓度为1.2 g/L时,最大清除率可高达95.4%。