BOD生物传感器微生物固定化技术研究进展

综述了微生物固定化技术在BOD微生物传感器上的研究应用进展，重点讨论了固定化微生物寿命和稳定性影响因素。     

生物传感器在水质分析监测中的应用

简要介绍了生物传感器的基本原理。重点介绍了生物传感器在水质分析监测中的应用,主要有生化需氧量的测定、细菌总数、硫化物、有机农药、酚的测定等。同时探讨了生物传感器存在的不足,并展望了生物传感技术的发展和应用前景。     

BOD微生物传感器的微生物膜性能研究

1 引言 1976年,Vernimmen提出了用氧电极接种活性污泥测定BOD的方法.其后Karube等将丝孢酵母菌用聚丙酰胺或骨胶原固定在多孔膜上,再将此微生物膜安装在氧电极的透气膜上构成BOD微生物传感器,这种方法能在15min左右测定污水的BOD值,结果和五天法一致.尽管国内外对BOD微生物传感器工作已有了不少报道,但其核心问题即生物膜的制备和菌种的选择与培养均未达到完善的阶段.为此,国际上商品化仪器仍然未能作为常规测定的工具而推广,大家都对这一核心问题,进行竞争性研究,直至开发出稳定优质的生物膜.以下就制生物膜常用的皮状丝孢酵母菌与活性污泥中的假单孢菌进行性能对比.     

BOD微生物传感器检测仪(BODs)研究进展

介绍了BOD微生物传感检测仪(BODs)的研究进展及应用前景。在分析BODs的工作原理及其在水质测定中的优点的基础上,重点介绍了其核心部分——微生物膜的研究现状以及制备过程中存在的问题。并根据BODs研究现状提出今后研究的重点:分离挑选广谱高效的微生物菌种、改进固定化技术及选择合适的固定化材料。     

微生物燃料电池传感器在水质毒性监测中的应用与发展

微生物燃料电池传感器是一种将微生物水处理与电化学传感相结合的新型分析检测技术,具有无需外加电源和信号转换装置即可实现自发连续监测的特点,在水质在线监测方面表现出巨大的应用前景。文中从微生物燃料电池传感器的工作原理入手,主要关注该技术在水体毒性检测领域的研究进展,重点解析了单/双室反应器构型和恒定外阻/恒定阳极电势运行模式的优势和不足,概述了微生物燃料电池传感器正常运行所需的最适溶解氧(DO)、温度以及溶液pH这些环境条件。进而着重介绍了响应时间、检出限、恢复速率以及信号重现性这些反映传感器灵敏度和稳定性的重要指标,探讨了优化传感器构型和运行模式、缩短水力停留时间、提升微生物活性对上述指标的积极影响,旨在获得提升微生物燃料电池传感器在毒性监测方面性能的有效方法,展望该技术在水质监测领域的前景及发展方向.     

高灵敏度生物传感器BOD快速测定仪的产业化进展

生化需氧量（biochemical oxygen demand,BOD）是评价水质有机物污染程度的综合指标,广泛应用于水体污染监测和污水处理的运行控制。目前BOD的测定在国内外均采用5天培养法,存在测定周期长、操作复杂等缺点。不能满足BOD在线快速监测的要求。高灵敏度生物传感器BOD快速测定仪利用固定化微生物颗粒作为BOD快速测定仪的全新生物识别元件,突破了采用生物膜作为生物识别元件的缺陷,     

生物传感器中酶的固定化方法

根据生物传感器及酶的特性,介绍了酶的固定化方法,比较了各种固定化方法的优劣.     

BOD快速测定仪使用综述

生化需氧量是目前国际上用来衡量水质污染程度的重要指标,但常规操作繁琐,并需五天时间。本文介绍一种以微生物电极为传感器的快速测定仪的原理、结构、注意事项和应用。与BOD5经典方法同步测定废水样品,结果相关性能良好,是一种具有实用价值的分析仪器。     

缩短BOD5常规测试时间的方法

从ＢＯＤ的特点和ＢＯＤ和ＢＯＤ５的相互关系,得到相关公式,通过实测数据和娄理统计方法进行了检验,表明该相关公式具有适用性。     

BOD生物探针仪的研究

采用海藻酸钙包埋异常汉逊酵母,将自行设计的简易流动注射式系统,结合溶氧电极,构成BOD 生物探针仪,对污水进行快速测定。电极响应与污水 BOD 之间有较明显的线性关系,对污水的响应可在30min 内完成,测量高达数千 mg/L BOD_5。固定化细胞的保存寿命在4周以上,测定误差在15%以内。     

Semi‐specific biosensors for measuring BOD in dairy wastewater

BACKGROUND: Semi‐specific microbial biochemical oxygen demand (BOD) biosensors were constructed using living cells of Escherichia coli R17.1.3 (E. coli) and Raoultella terrigena P74.3 (R. terrigena) immobilized in agarose gel matrix. The research involved comparison with the Pseudomonas fluorescens P75 (P. fluorescens) biosensor that had no specificity in decomposing lactose and milk derivates. The constructed BOD biosensors were calibrated with OECD synthetic wastewater and tested with different wastewater samples. RESULTS: The linear range of the calibration curve was 5–200 mg L^?1 BOD₇ for R. terrigena and P. fluorescens based biosensors and 150 mg L^?1 BOD₇ for the E. coli based biosensor. Service life was 60 days for E. coli and P. fluorescens based biosensors and 40 days for R. terrigena based biosensors. BOD values for dairy industry wastewater obtained with current semi‐specific biosensors considerably overestimate BOD₇, while universal biosensors underestimate BOD₇ obtained by the conventional 7‐day BOD test. CONCLUSION: In spite of extensive overestimation of BOD₇ the semi‐specific biosensors enabled better estimation of BOD in dairy industry wastewater than a reference P. fluorescens biosensor. The best result, in terms of service life, stability, sensitivity and reproducibility was accomplished with semi‐specific E.coli biosensor. Copyright © 2010 Society of Chemical Industry     

A novel microbial BOD biosensor developed by the immobilization of P. Syringae in micro‐cellular polymers

BACKGROUND: A biochemical oxygen demand (BOD) sensor, based on an immobilized Pseudomonas syringae in highly porous micro‐cellular polymer (MCP) in combination with a dissolved oxygen electrode, has been developed for the analysis of biodegradable organic compounds in aqueous samples. Microorganisms were immobilized in a molded MCP disk and a wastewater sample was injected into the biocomposite disk by a flow injection system. Dissolved oxygen (DO) changes as a measure of soluble BOD was read with a DO probe placed into a flow cell carrying biocatalytically activated disk. RESULTS: Optimal response of the MCP BOD sensor was obtained at pH 6.8 and 25 °C with a typical response time of 3–5 min for a 2 mm thick molded polymeric disk. The sensor showed detection linearity over the range 5–100 mg L^?1 BOD₅ (r² > 0.99) at a flow rate of 0.6 mL min^?1. The repeatability and reproducibility of the sensor response were found to be 3.08% and 7.77%, respectively. BOD values produced with this biosensor for various municipal and industrial wastewaters correlated well with those determined by the conventional 5‐day BOD test. CONCLUSION: This new biosensor was different from present amperometric BOD biosensor configurations in which the biocatalyst (microbial/enzymatic) is placed between cellulose and Teflon membranes installed on a DO probe. The use of a molded MCP disk coniainng microbial activity offers better stability and lifetime for commercial use in environmental monitoring. Copyright © 2008 Society of Chemical Industry     

微生物电极法BOD快速测定仪实验研究

对微生物电极法快速测定仪的应用进行实验研究,并与稀释接种法对水样中的生化需氧量的测定结果进行比较.     

冷却水系统中腐蚀和水质的现场监测

综述了冷却水系统中金属腐蚀的几种现场监测方法（试片法、试验管法、线性极化法和监测换热器法）、腐蚀控制的指标、与腐蚀控制密切相关的冷却水水质现场监测的内容及水质控制的指标。     

The effect of operating conditions on the response of a differential enzyme thermistor

The performance of a low cost differential enzyme thermistor system was studied. Initial results from physical characterisations showed the interdependence of operating parameters (flow rate and ambient temperature) on detector sensitivity. Subsequent work demonstrated the detection of urea using an immobilised urease column.     

贵阳地表水体中的TOC、BOD、COD相关性分析研究

通过对贵阳某地表水体TOC、COD和BOD进行检测经过数理统计回归分析,发现对于有机污染较严重的水体,TOC分别与CODcr、BOD5具有一定的相关性,可以根据测定的TOC值在一定范围内推算CODcr和BOD5的值。     

智能化监测换热设备在工业循环冷却水处理中的应用

结合工程实例,阐述了智能化监测换热设备的组成和工作原理,并根据使用状况分别作出效果评价.