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1.
牡蛎肉双酶复合水解工艺   总被引:2,自引:0,他引:2       下载免费PDF全文
以牡蛎为原料,利用多种蛋白酶对牡蛎蛋白质进行水解,挑选中性蛋白酶1398和Flavourzyme蛋白酶进行复合酶解,得出酶解的最佳工艺参数为:时间16h,中性蛋白酶1398与Flavourzyme作用的时间比为16∶15,中性蛋白酶1398酶与底物比为300U/g,Flavourzyme酶与底物比为1200U/g.水解后,氨基态氮质量分数为0.469%,总氮回收率为91%.  相似文献   

2.
以罗非鱼下脚料为原料,利用多种蛋白酶对罗非鱼下脚料中的蛋白质进行水解,挑选中性蛋白酶1398以及Flavourzyme两种蛋白酶进行复合酶解,得出酶解的最佳工艺参数为:中性蛋白酶1398的酶底比为300U/g、Flavourzyme的酶底比为1200 U/g、Flavourzyme的作用时间为15h、总时间为16h、底物浓度为2%.水解后,总氮回收率达到84.24%.  相似文献   

3.
以蟹肉为原料,利用多种蛋白酶对蟹肉中的蛋白质进行水解,挑选中性蛋白酶1398以及Flavourzyme两种蛋白酶进行复合酶解,得出酶解的最佳工艺参数为:中性蛋白酶1398的酶底比为300U/g、Flavourzyme的酶底比为1200U/g、Flavourzyme的作用时间为14.5h、总时间为16h、底物浓度为2%。水解后,总氮回收率达到81.82%。  相似文献   

4.
陈义勇  王伟  沈宗根  郁达 《食品科技》2006,31(12):70-73
以蟹肉为原料,利用多种蛋白酶对蟹肉中的蛋白质进行水解,挑选中性蛋白酶1398以及Flavourzyme两种蛋白酶进行复合酶解,得出酶解的最佳工艺参数为中性蛋白酶1398的酶底比为300U/g、Flavourzyme的酶底比为1200U/g、Flavourzyme的作用时间为14.5h、总时间为16h、底物浓度为2%。水解后,总氮回收率达到81.82%。  相似文献   

5.
本文对不同蛋白酶对脱脂油菜饼粕中蛋白质的水解作用及水解产物的风味作了初步研究。结果表明:在6种蛋白酶中,Alcalase的氮收率最高,其次是HAP碱性蛋白酶,1398中性蛋白酶,Flavourzyme,537酸性蛋白酶和木瓜白酶的较低;Flavourzyme的水解产物的水解度最高,而HAP碱性蛋白酶和1398中性蛋白酶水解产物的水解度较低。HAP碱性蛋白酶,Alcalase,1398中性蛋白酶的水解产物有较强的苦味,木瓜蛋白酶和537酸性蛋白酶水解产物口感平淡,Flavourzyme水解产物无苦味。  相似文献   

6.
本文对不同蛋白酶对脱脂油菜饼粕中蛋白质的水解作用及水解产物的风味作了初步研究.结果表明:在6种蛋白酶中,Alcalase的氮收率最高,其次是HAP碱性蛋白酶、1398中性蛋白酶、Flavourzvme,537酸性蛋白酶和木瓜蛋白酶的较低;Flavourzyme的水解产物的水解度最高,而HAP碱性蛋白酶和1398中性蛋白酶水解产物的水解度较低.HAP碱性蛋白酶、Alcalase、1398中性蛋白酶的水解产物有较强的苦味,木瓜蛋白酶和537酸性蛋白酶水解产物口感平淡,Flaavourzyme水解产物无苦味.  相似文献   

7.
为充分改善血红蛋白的感官质量并扩大其应用范围,本实验以猪血为原料,以猪血红蛋白为研究对象,采用6种食品级商业蛋白酶Alcalase 2.4L,胰蛋白酶,Flavourzyme,AS1398中性蛋白酶,木瓜蛋白酶及胃蛋白酶在各自最适反应条件下分别酶解猪血红蛋白480min,以产物色泽为评价指标,筛选出木瓜蛋白酶为最佳水解用酶。其酶解液接近无色,透明、清澈,感官质量得到了很大提升。其酶解条件为底物浓度5%,酶与底物比100U/g,温度37℃,pH值6.5。各蛋白酶水解液的色泽由浅至深依次为:木瓜蛋白酶,Flavourzyme,AS1398中性蛋白酶,胰蛋白酶,胃蛋白酶和Alcalase 2.4L。  相似文献   

8.
芝麻蛋白水解工艺的研究   总被引:1,自引:0,他引:1  
以芝麻为原料,利用多种蛋白酶对芝麻的蛋白质进行水解,挑选木瓜蛋白酶以及风味蛋白酶(Flavourzyme)两种蛋白酶进行复合酶解,得出酶解的最佳工艺参数为:木瓜蛋白酶的酶底比为600U/S,风味蛋白酶的酶底比为1200U/S,风味蛋白酶的作用时间为14.5h,总时间为16h,底物浓度为2%。水解后,总氮回收率达到55.73%。  相似文献   

9.
以豆粕为原料,利用多种蛋白酶对豆粕的蛋白质进行水解,挑选木瓜蛋白酶以及Flavourzyme两种蛋白酶进行复合酶解得出酶解的最佳工艺参数为:木瓜蛋白酶的酶底比为600U/g、Flavourzyme的酶底比为1200U/g、Flavourzyme的作用时间为14.5h、总时间为16h、底物浓度为2%。水解后,总氮回收率达到55.73%。  相似文献   

10.
酶解法综合制备海参多肽、多糖工艺研究   总被引:1,自引:0,他引:1  
付学军  朴亮均 《食品科技》2007,32(9):116-118
从综合利用海参各种营养成分的角度出发,研究了利用酶解法制备海参多肽的同时分离纯化海参多糖的工艺。通过正交实验得出利用A.S1398精制中性蛋白酶综合提取海参多肽和多糖的最优酶解条件:加水量为鲜海参质量的6倍、加酶量1.0%、温度30℃、水解时间6h。在该条件下多肽得率为12.0%,多糖得率为2.68%,水解度为30.35%。  相似文献   

11.
蛋白质水解度测定方法综述   总被引:27,自引:0,他引:27  
对目前国内外常用的蛋白质水解度测定方法进行了综述,其中pH—state方法是通过滴定水解过程中释放的质子测定DH;OPA、TNBS及国内常用的水合茚三酮和甲醛等测定方法是利用游离氨基的反应测定DH。  相似文献   

12.
SUMMARY: A partially purified immunoglobulin G (lgG) solution prepared from the serum of species to be tested was heated to the specifications for sausages. The resulting supernatant fluid was decanted and the precipitate washed with saline and used to immunize rabbits. The supernatant fluid was used to sensitize tanned sheep red blood cells. The immune serum was rendered monospecific by absorptions with heterologous, heated lgG precipitates. A sample of monospecific immune serum was absorbed with a washed homogenate of sausage. Aliquots of the monospecific immune serum, both untreated and sausage absorbed, were tested with cells sensitized with the homologous heated lgG supematant fluid. A significant reduction of titer by sausage absorption indicated that the sausages contained the meat homologous to the immune serum.  相似文献   

13.
研究了聚多元羧酸盐的合成方法及反应机理,将其应用于洗涤剂和PVC制品中分别代替三聚磷酸钠和邻苯二甲酸二丁酯,证明有良好效果。  相似文献   

14.
SUMMARY— The mechanism and generality of the known stabilization against autoxidation conferred on linoleic acid by certain basic amino acids, such as lysine and arginine, was investigated. Basic amino acids were the only class of compounds found to confer the effect. However, the smallest basic amino acid, 2,3-diaminopropionic acid, was not effective, nor was αβω-diaminc acid, 3,6-diaminohexanoic acid, although a simple isomer of lysine. The stabilization was observed only in the solid phase. Inclusion of sodium chloride in the solid matrix was deleterious to the effect. A large number of physical and chemical observations were made and correlated but it has not been possible to draw detailed conclusions about the mechanism of stabilization, nor can a detailed structure of the stabilized complex be suggested. The cause of the phenomenon appears to be closely associated with the physical arrangement of the ions in the crystal lattice.  相似文献   

15.
Hop oil emulsions prepared from different varieties of hops have been found to exhibit enhanced physical stability on the addition of blends of the emulsifiers Span 20/Tween 80 or Span 60/Tween 60. Examination of the particle size and volume distributions of an emulsion by use of a Coulter Counter was found to be an excellent method of monitoring its stability. An indication as to the relative efficiency of emulsifiers can be obtained from Coulter Counter measurements on hop oil emulsions after storage for 4 days. The use of an ultracentrifuge provies a rapid means of testing emulsion stability and hence the effectiveness of emulsion stabilizers.  相似文献   

16.
百年风尚     
《中国服装(北京)》2013,(19):130-133
一场流光溢彩、赏心悦目的展览,一段百年风尚演进的传奇旅程,一次东西方文化艺术的完美对话。2013年9月13日,“博萃臻艺一中西方珍宝艺术展”在辽宁省博物馆举行了隆重的开幕仪式,法兰西共和国驻华大使白林女士、辽宁省文物局局长丁辉先生、辽宁省博物馆馆长马宝杰先生、卡地亚全球总裁兼首席执行官邓阁仕先生、卡地亚区域行政总裁(北亚洲)陆慧全先生、卡地亚中国区首席执行官陆意斯先生、辽宁省文物店总经理张春鹰先生,以及众多文化界与文博界的贵宾齐聚一堂,共同见证了这场文化艺术盛事。  相似文献   

17.
The lengths of chains of cells of Saccharomyces cerevisiae were studied during fermentation. Pitching yeast generally contained about half of the total number of cells as two-celled chains. The chain lengths varied during the subsequent fermentation and the variations were characteristic of the strain. Electronic counting assessments of chain length were unreliable.  相似文献   

18.
The quantitative analysis of the reaction products of the water activity dependent nonenzymatic glycosylation of lysozyme was not straightforward. Difficulties arose in the determination of the number of bound glucose molecules because glycosylation leads to glucose mediated protein aggregation, and the likely presence of a mixture of relatively labile Schiff-base intermediates, and the more stable ketoamine products generated by Amadori rearrangement. Polyacrylamide gel electrophoresis was used to monitor protein aggregation; periodate oxidation, nuclear magnetic resonance (NMR), and oxalic acid hydrolysis combined with HPLC, emerged as the most promising methods to quantitate the degree of glycosylation. Possible interpretations are advanced to explain the apparent discrepancies in degree of glycosylation suggested by the different analytical methods evaluated.  相似文献   

19.
ABSTRACT— The effect of time, temperature and rpm of comminution of emulsions was determined on the dispersion of approximately 25% of beef fat, pork fat or cottonseed oil in frankfurters. The numbers of lipid particles 5 μ or less in diameter increased in frankfurters containing either beef or pork fat as comminution was continued to higher temperatures, with pork fat dispersed more thoroughly. Fat tended to separate from frankfurters containing beef fat in particles 200 μ or more in diameter. In contrast, no specific degree of dispersion of particles 5 μ or less in diameter consistently indicated emulsion stability, or its lack. Increased rpm during comminution produced an increased dispersion of beef or pork fat. Under the same conditions pork fat was dispersed more finely than beef fat. Dispersion of cottonseed oil produced finely dispersed particles beyond the resolution of light microscopy, as was confirmed by electron microscopy which showed a substantial number of particles to be less than 1 μ in diameter.  相似文献   

20.
矩阵乘积的行式,列式   总被引:1,自引:0,他引:1  
给出了m×m矩阵与m×n矩阵的行(列)式的表达式.若A=a11a12…a1ma21a22…a2m……am1am2…ammB=b11b12…b1nb21b22…b2n……bm1bm2…bmn分别是m×m,m×n矩阵,则|A||B|=|AB|+∑i1<i2<…<itj1<j2<…<jt1≤t≤mn-t≥mNBi1i2…itj1j2…jtNAB1…m(-1)st+1jt+1…(-1)snjn其中i1,i2,…,it是1,2,…,m中t个数码;j1,j2,…,jt,jt+1,…,jn是1,2,…,n的一个排列;sr=π(j1,j2,…,jt,jr)(r=1,2,…,n)是排列j1,j2,…,jt,jr的反序数.  相似文献   

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