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1.
Distillery effluent poses severe environmental pollution problem mainly due to its high organic content. During alcohol fermentation, most of the essential macro- and micro-nutrients get utilized. Therefore, supplementation of these nutrients becomes imperative for the improvement of biohydrogen production. In the present study, starch based distillery effluent was used for dark fermentative hydrogen production using Enterobacter cloacae IIT-BT 08. Hence, this study was undertaken to evaluate the effect of supplementation of yeast extract, malt extract, Fe++, Cu++ and Mg++ on biohydrogen production. The interaction among supplements and their mutual effect on the hydrogen production was studied using five factor–five level central composite design). Optimum hydrogen yield of 7.4 mol H2/kg CODreduced was predicted by the model, which showed an excellent correlation with experimental hydrogen yield of 7.38 ± 0.24 mol H2/kg CODreduced. An average hydrogen production rate of 80 mL/L h was achieved after supplementation, having 2.2 times higher hydrogen yield as compared to non-supplemented distillery effluent. 相似文献
2.
Enterobacter cloacae IIT-BT 08 was found to harbor multiple endogenous plasmids. The plasmids were successfully cured by the combined action of SDS and mitomycin C. The cured strain exhibited an altered pattern of antibiotic and metal resistance. The effect of plasmid curing on biohydrogen production was determined. LP model showed that hydrogen was a growth associated product for both the wild type and the cured strain. However, modified Gompertz equation showed that the productivity of the cured strain was comparable to the wild type. Further, comparative kinetic parameter analysis showed that the maximum specific growth rate (μmax) and saturation constant (Ks) were 1.26 and 2.2 times higher respectively, for the cured strain as compared to the wild type. Similarly, maintenance coefficient (m) was determined to be function of fermentation time and was lower for the cured strain. This was related to the decrease in plasmid load in the cured strain. 相似文献
3.
4.
Biological H2 production has an edge over its chemical counterpart mainly because it is environmentally benign. Despite having simpler technology, higher evolution rate of H2 and the wide spectrum of substrate utilization, the major deterrent of anaerobic dark fermentation process stems from its lower achievable yields. Theoretically, the maximum H2 yield is 4 mol H2/mol glucose when glucose is completely metabolized to acetate or acetone in the anaerobic process. But it is somewhat difficult to achieve the complete degradation of glucose to carbon dioxide and H2 through anaerobic dark fermentation. Moreover, this yield appears too low to be economically viable as an alternative to the existing chemical or electrochemical processes of hydrogen generation. Intensive research studies have already been carried out on the advancement of these processes, such as the development of genetically modified microorganism, improvement of the reactor designs, use of different solid matrices for the immobilization of whole cells, development of two-stage processes, and higher H2 production rates. Maximum H2 yield is found to be 5.1 mol H2/mol glucose. However, major bottlenecks for the commercialization of these processes are lower H2 yield and rate of H2 production. Competent microbial cultures are required to handle waste materials efficiently, which are usually complex in nature. This will serve dual purposes: clean energy generation and bioremediation. Scale-up studies on fermentative H2 production processes have been done successfully. Pilot plant trials of the photo-fermentation processes require more attention. Use of cheaper raw materials and efficient biological H2 production processes will surely make them more competitive with the conventional H2 generation processes in near future. 相似文献
5.
Namita KhannaChitralekha Nag Dasgupta Preeti MishraDebabrata Das 《International Journal of Hydrogen Energy》2011,36(24):15573-15582
The present study investigated the influence of increase in intracellular [FeFe] hydrogenase levels, in Enterobacter cloacae IIT-BT 08, on the formation of molecular hydrogen. The hydA gene from E. cloacae IIT-BT 08 was successfully amplified and cloned downstream of a tac promoter in an Escherichiacoli-Enterobacter reconstructed pGEX-Kan shuttle vector and introduced into E. cloacae. Finally E. cloacae strain carrying multiple copies of pGEX-Kan-hydA vector was developed. Homologous overexpression of the [FeFe] hydrogenase gene increased the hydrogenase activity by1.3-fold as compared to the wild type. SDS-PAGE confirmed the successful expression of the GST-tagged hydA protein. The hydrogen yield and rate of production in recombinant strain were found to be 1.2-fold and 1.6-fold higher, respectively, compared to the wild type strain. This was found to be concomitant with the shift in the metabolic pathway. In addition, feasibility of using cheese whey as a substrate for biohydrogen production and the effect of its supplementation with yeast extract as nitrogen source was studied for both the wild type and the recombinant strain. It was found that supplementation with 0.3% (w/v) yeast extract enhanced hydrogen production from whey. Further, the yield and rate of hydrogen production from the recombinant was found to be more promising as compared to the wild type. 相似文献
6.
Sundaresan Mohanraj Shanmugam Kodhaiyolii Mookan Rengasamy Velan Pugalenthi 《International Journal of Hydrogen Energy》2014
The effects of FeSO4 and synthesized iron oxide nanoparticles (0–250 mg/L) on fermentative hydrogen production from glucose and sucrose, using Enterobacter cloacae were investigated, to find out the enhancement of efficiency. The maximum hydrogen yields of 1.7 ± 0.017 mol H2/mol glucose and 5.19 ± 0.12 mol H2/mol sucrose were obtained with 25 mg/L of ferrous iron supplementation. In comparison, the maximum hydrogen yields of 2.07 ± 0.07 mol H2/mol glucose and 5.44 ± 0.27 mol H2/mol sucrose were achieved with 125 mg/L and 200 mg/L of iron oxide nanoparticles, respectively. These results indicate that the enhancement of hydrogen production on the supplementation of iron oxide nanoparticles was found to be considerably higher than that of ferrous iron supplementation. The activity of E. cloacae in a glucose and sucrose fed systems was increased by the addition of iron oxide nanoparticles, but the metabolic pathway was not changed. The results revealed that the glucose and sucrose fed systems conformed to the acetate/butyrate fermentation type. 相似文献
7.
Sanjukta Subudhi Tanmaya Nayak N. Ram KumarP. Vijayananth Banwari Lal 《International Journal of Hydrogen Energy》2013
A hydrogen producing facultative anaerobic alkaline tolerant novel bacterial strain was isolated from crude oil contaminated soil and identified as Enterobacter cloacae DT-1 based on 16S rRNA gene sequence analysis. DT-1 strain could utilize various carbon sources; glycerol, CMCellulose, glucose and xylose, which demonstrates that DT-1 has potential for hydrogen generation from renewable wastes. Batch fermentative studies were carried out for optimization of pH and Fe2+ concentration. DT-1 could generate hydrogen at wide range of pH (5–10) at 37 °C. Optimum pH was; 8, at which maximum hydrogen was obtained from glucose (32 mmol/L), when used as substrate in BSH medium containing 5 mg/L Fe2+ ion. Decrease in hydrogen partial pressure by lowering the total pressure in the fermenter head space, enhanced the hydrogen production performance of DT-1 from 32 mmol H2/L to 42 mmol H2/L from glucose and from 19 mmol H2/L to 33 mmol H2/L from xylose. Hydrogen yield efficiency (HY) of DT-1 from glucose and xylose was 1.4 mol H2/mol glucose and 2.2 mol H2/mol xylose, respectively. Scale up of batch fermentative hydrogen production in proto scale (20 L working volume) at regulated pH, enhanced the HY efficiency of DT-1 from 2.2 to 2.8 mol H2/mol xylose (1.27 fold increase in HY from laboratory scale). 84% of maximum theoretical possible HY efficiency from xylose was achieved by DT-1. Acetate and ethanol were the major metabolites generated during hydrogen production. 相似文献
8.
Alissara Reungsang Sureewan Sittijunda Sompong O-thong 《International Journal of Hydrogen Energy》2013
Bio-hydrogen production from glycerol by immobilized Enterobacter aerogenes ATCC 13048 on heat-treated upflow anaerobic sludge blanket (UASB) granules was examined in a UASB reactor. The organic loading rate (OLR) was optimized in order to maximize the hydrogen production rate (HPR). The maximum hydrogen content (37.1% and 24.2%) and HPR (9 and 6.2 mmol H2/L h) were achieved at the optimum OLR of 50 g/L d using pure and waste glycerol as the substrate, respectively. The major soluble metabolite products (SMPs) were ethanol, 1,3-propanediol (1,3-PD), formic acid, and acetic acid. The microbial community and microbial structure, analyzed by fluorescent in situ hybridization (FISH) and scanning electron microscopy (SEM), revealed that the predominant hydrogen producers were E. aerogenes ATCC 13048 and firmicutes bacteria including Clostridium, Bacillus, and Dialister sp. 相似文献
9.
Vanita Roshan NimjeChien-Yen Chen Chien-Cheng ChenJi-Yi Tsai Hau-Ren ChenYuh Ming Huang Jiin-Shuh JeanYoung-Fo Chang Ruey-Chyuan Shih 《International Journal of Hydrogen Energy》2011,36(17):11093-11101
The aim of this study was to evaluate the electrical generating characteristics of a double-chamber microbial fuel cell with pure culture of Enterobacter cloacae as a function of pH variation of its microenvironment. The performance was analyzed over 5 batch cycles (around 16-21 days) with community wastewater by adjusting the pH between 6.5 and 9.5. Operations under pH 6.5 (0.40 mA) and 7.4 (0.42 mA) showed highly effective performance with respect to maximum current generation and maximum power density corresponding to pH 8.5 (0.38 mA) and 9.5 (0.27 mA). This better performance could be attributed to the low internal resistance under the low pH microenvironment. Short experiments conducted for 60 min with different external resistances to calculate maximum current and internal resistance were remarkably shown to increase current and decrease internal resistance with respect to pH 6.5 and 7.4. Maximum power density obtained from the polarization curve was observed to follow the same behavior as current generation with a maximum of 0.0042 mW/cm2 for pH 7.4. COD removal efficiencies increased as a function of pH, and maximum amounted to pH 9.5 respectively, due to long operating time. Coulombic efficiency attained different trend with a maximum of 3.4% at pH 6.5. Low pH of 6.5 and 7.4 were associated with dominant electrochemical activity, which was proved by cyclic voltammetry. These results demonstrate the importance of pH environment in the biocurrent generation with wastewater containing E. cloacae. 相似文献
10.
Sergei A. Markov Jared AverittBarbara Waldron 《International Journal of Hydrogen Energy》2011,36(1):262-266
Glycerol was used as a substrate for H2 production by bacterium Enterobacter aerogenes in the test tubes and bioreactor. A BioFlo/CelliGen 115 bioreactor (10 L working volume) was utilized to conduct the experiments for conversion of glycerol into H2 by E. aerogenes cells. The highest H2 production rate was observed under 2% glycerol in the culture medium. The glycerol uptake efficiency by bacteria in the bioreactor was found to be 65% during the 6 day period, matching glycerol uptake efficiency observed in the test tubes experiment (65%).Hydrogen production from glycerol (2% glycerol, v/v) by E. aerogenes in the bioreactor and test tubes was measured over the 6 days, showing the maximal H2 rate at 650 mL g−1 dry weight h−1. The yield of H2 production from glycerol at 0.89 mol/mol in the bioreactor was high, corresponding to the theoretical yield of 1 mol of H2 per 1 mol of glycerol. 相似文献
11.
Tami Astie Ulhiza Noor Illi Mohamad Puad Azlin Suhaida Azmi 《International Journal of Hydrogen Energy》2018,43(49):22148-22158
Sago wastewater (SWW) causes pollution to the environment due to its high organic content. Annually, about 2.5 million tons of SWW is produced in Malaysia. In this study, the potential of SWW as a substrate for biohydrogen production by Enterobacter aerogenes (E. aerogenes) was evaluated. Response Surface Methodology (RSM) was employed to find the optimum conditions. From preliminary optimization, it was found that the most significant factors were yeast extract, temperature, and inoculum size. According to Face Centered Central Composite Design (FCCCD), the maximum hydrogen concentration and yield were 630.67 μmol/L and 7.42 mmol H2/mol glucose, respectively, which is obtained from the sample supplemented with 4.8 g/L yeast extract concentration, 5% inoculum, and incubated at the temperature of 31 °C. Cumulative hydrogen production curve fitted by the modified Gompertz equation suggested that Hmax, Rmax, and λ from this study were 15.10 mL, 2.18 mL/h, and 9.84 h, respectively. 相似文献
12.
A mesophilic high hydrogen producing strain DMHC-10 was isolated from a lab scale anaerobic reactor being operated on distillery wastewater for hydrogen production. DMHC-10 was identified as Clostridium sp. on the basis of 16S rRNA gene sequencing. Various medium components (carbon and nitrogen sources) and environmental factors (initial pH, temperature of incubation) were optimized for hydrogen production by Clostridium sp. DMHC-10. The strain, in late exponential growth phase, showed maximum hydrogen production (3.35 mol-H2 mol−1 glucose utilized) at 37 °C, pH 5.0 in a medium supplemented with organic nitrogen source. Butyric acid to acetic acid ratio was ca. 2.3. Hydrogen production declined when organic nitrogen was replaced with inorganic nitrogen. 相似文献
13.
Simultaneous production of hydrogen and ethanol from waste glycerol by Enterobacter aerogenes KKU-S1
Alissara Reungsang Sureewan Sittijunda Irini Angelidaki 《International Journal of Hydrogen Energy》2013
Factors affecting simultaneous hydrogen and ethanol production from waste glycerol by a newly isolated bacterium Enterobacter aerogenes KKU-S1 were investigated employing response surface methodology (RSM) with central composite design (CCD). The Plackett-Burman design was first used to screen the factors influencing simultaneous hydrogen and ethanol production, i.e., initial pH, temperature, amount of vitamin solution, yeast extract (YE) concentration and glycerol concentration. Results indicated that initial pH, temperature, YE concentration, and glycerol concentration had a statistically significant effect (p ≤ 0.05) on hydrogen production rate (HPR) and ethanol production. The significant factors were further optimized using CCD. Optimum conditions for simultaneously maximizing HPR and ethanol production were YE concentration of 1.00 g/L, glycerol concentration of 37 g/L, initial pH of 8.14, and temperature of 37 °C in which a maximum HPR and ethanol production of 0.24 mmol H2/L h and 120 mmol/L were achieved. 相似文献
14.
Jenni J. Seppälä Jaakko A. PuhakkaOlli Yli-Harja Matti T. KarpVille Santala 《International Journal of Hydrogen Energy》2011,36(17):10701-10708
The effect of coculture of Clostridium butyricum and Escherichia coli on hydrogen production was investigated. C. butyricum and E. coli were grown separately and together as batch cultures. Gas production, growth, volatile fatty acid production and glucose degradation were monitored. Whilst C. butyricum alone produced 2.09 mol-H2/mol-glucose the coculture produced 1.65 mol-H2/mol-glucose. However, the coculture utilized glucose more efficiently in the batch culture, i.e., it was able to produce more H2 (5.85 mmol H2) in the same cultivation setting than C. butyricum (4.62 mmol H2), before the growth limiting pH was reached. 相似文献
15.
Jong-Hwan Shin Jong Hyun Yoon Eun Kyoung Ahn Mi-Sun Kim Sang Jun Sim Tai Hyun Park 《International Journal of Hydrogen Energy》2007
A new fermentative hydrogen-producing bacterium was isolated from a domestic landfill and identified as Enterobacter asburiae using 16S rRNA gene sequencing and DNA–DNA hybridization methods. The isolated bacterium, designated as Enterobacter asburiae SNU-1, is a new species that has never been examined as a potential hydrogen-producing bacterium. This study examined the hydrogen-producing ability of Enterobacter asburiae SNU-1. During fermentation, the hydrogen was mainly produced in the stationary phase. The hydrogen yield based on the formate consumption was 0.43 mol hydrogen/mol formate. This strain was able to produce hydrogen over a wide range of pH (4–7.5), with the optimum pH being pH 7. The level of hydrogen production was also affected by the initial glucose concentration, and the optimum value was found to be 25 g glucose/l. The maximum and overall hydrogen productivities were 398 and 174 ml/l/hr, respectively, at pH 7 with an initial glucose concentration of 25 g/l. This strain could produce hydrogen from glucose and many other carbon sources such as fructose, sucrose, and sorbitol. 相似文献
16.
Experiments involving the addition of external nicotinamide adenine dinucleotide, reduced form (NADH) or nicotinamide adenine dinucleotide (NAD+) have been designed to examine how the hydrogen in Enterobacter aerogenes is liberated by NADH or NAD+. The addition of external NADH or NAD+ was found to regulate hydrogen production by E. aerogenes in resting cells, batch cultures, and chemostat cultures. Particularly in chemostat cultivation, with the external addition of NADH, hydrogen production via the NADH pathway was decreased, while that via the formate pathway was increased; in the end, the overall hydrogen p was decreased. The addition of NAD+, on the other hand, gave the opposite results. The membrane-bound hydrogenase was found to play a central role in regulating hydrogen production. The occurrence of NADH oxidation (NAD+ reduction) on the cell membrane resulted in an electron flow across the membrane; this changed the oxidation state and metabolic pattern of the cells, which eventually affected the hydrogen evolution. 相似文献
17.
Sanjay K.S. Patel Prasun Kumar Sanjeet Mehariya Hemant J. Purohit Jung-Kul Lee Vipin C. Kalia 《International Journal of Hydrogen Energy》2014
Defined co-cultures of hydrogen (H2) producers belonging to Citrobacter, Enterobacter, Klebsiella and Bacillus were used for enhancing the efficiency of biological H2 production. Out of 11 co-cultures consisting of 2–4 strains, two co-cultures composed of Bacillus cereus EGU43, Enterobacter cloacae HPC123, and Klebsiella sp. HPC793 resulted in H2 yield up to 3.0 mol mol−1 of glucose. Up-scaling of the reactor by 16-fold resulted in a corresponding increase in H2 production with an actual evolution of 7.44 L of H2. It constituted 58.2% of the total biogas. Continuous culture evolution of H2 by co-cultures (B. cereus EGU43 and E. cloacae HPC123) immobilized on ligno-cellulosic materials resulted in 6.4-fold improvement in H2 yield compared to free floating bacteria. This synergistic influence of B. cereus and E. cloacae can offer a better strategy for H2 production than undefined or mixed cultures. 相似文献
18.
Luis Manuel Rosales-Colunga Elías Razo-Flores Leandro G. Ordoñez Felipe Alatriste-Mondragón Antonio De León-Rodríguez 《International Journal of Hydrogen Energy》2010
This study reports a fermentative hydrogen production by Escherichia coli using cheese whey as substrate. To improve the biohydrogen production, an E. coli ΔhycA ΔlacI strain (WDHL) was constructed. The absence of hycA and lacI genes had a positive effect on the biohydrogen production. The strain produced 22% more biohydrogen in a shorter time than the wild-type (WT) strain. A Box-Behnken experimental design was used to optimize pH, temperature and substrate concentration. The optimal initial conditions for biohydrogen production by WDHL strain were pH 7.5, 37 °C and 20 g/L of cheese whey. The specific production rate was improved from 3.29 mL H2/optical density at 600 nm (OD600nm) unit-h produced by WDHL under non-optimal conditions to 5.88 mL H2/OD600nm unit-h under optimal conditions. Using optimal initial conditions, galactose can be metabolized by WDHL strain. The maximum yield obtained was 2.74 mol H2/mol lactose consumed, which is comparable with the yield reached in other hydrogen production processes with Clostridium sp. or mixed cultures. 相似文献
19.
Thermophilic dark fermentative hydrogen producing bacterial strain, TERI S7, isolated from an oil reservoir flow pipeline located in Mumbai, India, showed 98% identity with Thermoanaerobacterium thermosaccharolyticum by 16S rRNA gene analysis. It produced 1450–1900 ml/L hydrogen under both acidic and alkaline conditions; at a temperature range of 45–60 °C. The maximum hydrogen yield was 2.5 ± 0.2 mol H2/mol glucose, 2.2 ± 0.2 mol H2/mol xylose and 5.2 ± 0.2 mol H2/mol sucrose, when the respective sugars were used as carbon source. The cumulative hydrogen production, hydrogen production rate and specific hydrogen production rate by the strain TERI S7 with sucrose as carbon source was found to be 1704 ± 105 ml/L, 71 ± 6 ml/L/h and 142 ± 13 ml/g/h respectively. Major soluble metabolites produced during fermentation were acetic acid and butyric acid. The strain TERI S7 was also observed to produce hydrogen continuously up to 48 h at pH 3.9. 相似文献
20.
Siang Chen Wu Pi Fen Lu Yu Chien Lin Pei Chung Chen Chi Mei Lee 《International Journal of Hydrogen Energy》2012
Photobiological H2 production is a promising method for renewable energy development. An innovative system that co-cultivating Rhodopseudomonas palustris WP3-5 and Anabaena sp. CH3 was carried out to estimate the effect of co-cultivation on H2 production enhancement. H2 production prolongation and enhancement were observed due to the light and metabolic compatibility of these two strains. Co-culture system served by acetate and fructose as carbon source can accumulate H2 in 140.8 mL, almost double than the sum of individuals. Moreover, the enhancement of H2 production was significantly affected by the mixed ratio of two strains. The mixed ratio (WP3-5:CH3) of 1:2 showed a highest H2 production rate in 44.8 mL-H2/L-culture/h, and both 2:1 and 1:2 exhibited a relatively high substrate conversion efficiency during the latest period of cultivation, whereas the mixed ratio of 1:1 and 3:1 only revealed a prolongation in H2 production due to metabolic compatibility of two strains. 相似文献